Treponema saccharophilum DSM 2985

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema saccharophilum

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H7EM01|H7EM01_9SPIO CdiI_4 domain-containing protein OS=Treponema saccharophilum DSM 2985 OX=907348 GN=TresaDRAFT_1301 PE=4 SV=1
MM1 pKa = 7.36FEE3 pKa = 4.9KK4 pKa = 10.29FTGADD9 pKa = 3.3KK10 pKa = 11.39NFLNGFEE17 pKa = 3.9QLIGFEE23 pKa = 4.55LPADD27 pKa = 3.7YY28 pKa = 11.47SNFLLNTNGGTFKK41 pKa = 10.67EE42 pKa = 4.52LEE44 pKa = 4.09DD45 pKa = 4.26SFLLKK50 pKa = 10.92NIGEE54 pKa = 4.33QIFIDD59 pKa = 3.9CLFGFRR65 pKa = 11.84EE66 pKa = 4.06EE67 pKa = 4.98RR68 pKa = 11.84SLNLCNWYY76 pKa = 10.17KK77 pKa = 10.52EE78 pKa = 4.3YY79 pKa = 10.81EE80 pKa = 4.18LEE82 pKa = 4.59LPQNTIIIGNTYY94 pKa = 10.17GAGLIILIWDD104 pKa = 3.6EE105 pKa = 4.04NAKK108 pKa = 10.18GVYY111 pKa = 9.63LWDD114 pKa = 5.06DD115 pKa = 3.67SLEE118 pKa = 4.39LEE120 pKa = 4.49QSTEE124 pKa = 3.9EE125 pKa = 4.17CCTYY129 pKa = 10.78KK130 pKa = 9.42ITEE133 pKa = 4.17TFEE136 pKa = 5.67DD137 pKa = 5.13FMNLLNINN145 pKa = 3.67

Molecular weight: 16.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H7EI11|H7EI11_9SPIO Uncharacterized protein OS=Treponema saccharophilum DSM 2985 OX=907348 GN=TresaDRAFT_2567 PE=4 SV=1
MM1 pKa = 7.08EE2 pKa = 5.4QILILVGTSTLQTLAMVFFSTIFAVIIGFPLGILLNATAEE42 pKa = 4.23RR43 pKa = 11.84GITPHH48 pKa = 6.82PMLNMVLSRR57 pKa = 11.84VIDD60 pKa = 3.54VFRR63 pKa = 11.84SFPFVILMIVMLPFTRR79 pKa = 11.84LVLKK83 pKa = 9.13TVVGTAPTIIPLSFAAAPFIARR105 pKa = 11.84VTEE108 pKa = 4.07SALNEE113 pKa = 4.19VPEE116 pKa = 4.5GMVQAARR123 pKa = 11.84AMGSTNWQIIRR134 pKa = 11.84KK135 pKa = 8.79VLVPEE140 pKa = 4.61ALPSIVSGLTLTVINLVGCSAMAGTLGGGGLGDD173 pKa = 4.21LAIQYY178 pKa = 10.22GYY180 pKa = 11.2LRR182 pKa = 11.84FRR184 pKa = 11.84TDD186 pKa = 2.57VMIAAVVAIIIMVALIQFAGTSISNRR212 pKa = 11.84MMRR215 pKa = 11.84KK216 pKa = 8.97RR217 pKa = 3.41

Molecular weight: 23.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2828

0

2828

1013328

31

4175

358.3

39.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.529 ± 0.058

1.398 ± 0.021

5.967 ± 0.038

7.135 ± 0.048

5.049 ± 0.038

7.398 ± 0.049

1.396 ± 0.018

6.726 ± 0.037

6.721 ± 0.042

8.293 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.023

4.244 ± 0.037

3.411 ± 0.027

2.485 ± 0.027

4.849 ± 0.047

7.719 ± 0.057

5.156 ± 0.051

6.876 ± 0.045

0.973 ± 0.016

3.205 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski