Amino acid dipepetide frequency for Corynebacterium phage Juicebox

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.982AlaAla: 19.982 ± 2.231
0.912AlaCys: 0.912 ± 0.299
8.205AlaAsp: 8.205 ± 0.715
9.801AlaGlu: 9.801 ± 0.872
3.191AlaPhe: 3.191 ± 0.352
11.548AlaGly: 11.548 ± 1.511
2.431AlaHis: 2.431 ± 0.446
4.635AlaIle: 4.635 ± 0.632
4.787AlaLys: 4.787 ± 0.543
9.345AlaLeu: 9.345 ± 0.825
3.951AlaMet: 3.951 ± 0.578
3.571AlaAsn: 3.571 ± 0.598
6.838AlaPro: 6.838 ± 0.817
3.799AlaGln: 3.799 ± 0.816
10.105AlaArg: 10.105 ± 1.066
5.622AlaSer: 5.622 ± 0.739
8.585AlaThr: 8.585 ± 0.911
7.674AlaVal: 7.674 ± 0.934
2.431AlaTrp: 2.431 ± 0.382
2.127AlaTyr: 2.127 ± 0.34
0.0AlaXaa: 0.0 ± 0.0
Cys
0.836CysAla: 0.836 ± 0.325
0.228CysCys: 0.228 ± 0.133
0.38CysAsp: 0.38 ± 0.179
0.456CysGlu: 0.456 ± 0.197
0.152CysPhe: 0.152 ± 0.1
0.836CysGly: 0.836 ± 0.278
0.076CysHis: 0.076 ± 0.095
0.228CysIle: 0.228 ± 0.121
0.076CysLys: 0.076 ± 0.077
0.38CysLeu: 0.38 ± 0.184
0.228CysMet: 0.228 ± 0.23
0.228CysAsn: 0.228 ± 0.129
0.38CysPro: 0.38 ± 0.176
0.152CysGln: 0.152 ± 0.118
0.912CysArg: 0.912 ± 0.296
0.532CysSer: 0.532 ± 0.179
0.38CysThr: 0.38 ± 0.215
0.228CysVal: 0.228 ± 0.137
0.076CysTrp: 0.076 ± 0.084
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
9.041AspAla: 9.041 ± 0.65
0.76AspCys: 0.76 ± 0.253
5.166AspAsp: 5.166 ± 0.629
5.166AspGlu: 5.166 ± 0.827
1.823AspPhe: 1.823 ± 0.384
7.218AspGly: 7.218 ± 0.612
1.52AspHis: 1.52 ± 0.402
1.747AspIle: 1.747 ± 0.321
1.671AspLys: 1.671 ± 0.355
5.318AspLeu: 5.318 ± 0.611
1.292AspMet: 1.292 ± 0.372
1.596AspAsn: 1.596 ± 0.374
5.014AspPro: 5.014 ± 0.714
1.368AspGln: 1.368 ± 0.276
4.255AspArg: 4.255 ± 0.552
3.419AspSer: 3.419 ± 0.566
3.723AspThr: 3.723 ± 0.384
4.559AspVal: 4.559 ± 0.588
1.52AspTrp: 1.52 ± 0.306
1.671AspTyr: 1.671 ± 0.382
0.0AspXaa: 0.0 ± 0.0
Glu
7.902GluAla: 7.902 ± 0.729
0.228GluCys: 0.228 ± 0.123
2.963GluAsp: 2.963 ± 0.487
4.559GluGlu: 4.559 ± 0.708
1.899GluPhe: 1.899 ± 0.4
3.875GluGly: 3.875 ± 0.456
1.823GluHis: 1.823 ± 0.397
2.811GluIle: 2.811 ± 0.506
2.659GluLys: 2.659 ± 0.73
6.306GluLeu: 6.306 ± 0.794
1.747GluMet: 1.747 ± 0.38
2.203GluAsn: 2.203 ± 0.395
3.343GluPro: 3.343 ± 0.59
2.811GluGln: 2.811 ± 0.373
3.951GluArg: 3.951 ± 0.651
3.799GluSer: 3.799 ± 0.429
4.711GluThr: 4.711 ± 0.507
4.938GluVal: 4.938 ± 0.68
1.292GluTrp: 1.292 ± 0.394
1.52GluTyr: 1.52 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.507PheAla: 2.507 ± 0.445
0.076PheCys: 0.076 ± 0.081
2.583PheAsp: 2.583 ± 0.383
1.216PheGlu: 1.216 ± 0.404
0.836PhePhe: 0.836 ± 0.197
2.963PheGly: 2.963 ± 0.455
0.608PheHis: 0.608 ± 0.193
1.292PheIle: 1.292 ± 0.34
0.532PheLys: 0.532 ± 0.165
1.368PheLeu: 1.368 ± 0.285
0.532PheMet: 0.532 ± 0.184
0.608PheAsn: 0.608 ± 0.195
1.52PhePro: 1.52 ± 0.387
0.608PheGln: 0.608 ± 0.311
1.596PheArg: 1.596 ± 0.378
1.14PheSer: 1.14 ± 0.298
1.899PheThr: 1.899 ± 0.404
1.671PheVal: 1.671 ± 0.385
0.152PheTrp: 0.152 ± 0.092
0.836PheTyr: 0.836 ± 0.217
0.0PheXaa: 0.0 ± 0.0
Gly
8.509GlyAla: 8.509 ± 1.221
0.912GlyCys: 0.912 ± 0.304
5.242GlyAsp: 5.242 ± 0.679
5.85GlyGlu: 5.85 ± 0.518
1.899GlyPhe: 1.899 ± 0.462
6.002GlyGly: 6.002 ± 0.874
2.127GlyHis: 2.127 ± 0.484
4.255GlyIle: 4.255 ± 0.545
2.887GlyLys: 2.887 ± 0.584
6.382GlyLeu: 6.382 ± 0.693
1.899GlyMet: 1.899 ± 0.326
2.127GlyAsn: 2.127 ± 0.354
4.711GlyPro: 4.711 ± 0.639
3.647GlyGln: 3.647 ± 0.64
6.306GlyArg: 6.306 ± 0.741
4.027GlySer: 4.027 ± 0.768
5.47GlyThr: 5.47 ± 0.669
6.99GlyVal: 6.99 ± 0.711
2.279GlyTrp: 2.279 ± 0.377
1.292GlyTyr: 1.292 ± 0.309
0.0GlyXaa: 0.0 ± 0.0
His
2.051HisAla: 2.051 ± 0.413
0.076HisCys: 0.076 ± 0.083
1.747HisAsp: 1.747 ± 0.389
1.14HisGlu: 1.14 ± 0.381
0.912HisPhe: 0.912 ± 0.232
1.671HisGly: 1.671 ± 0.378
0.304HisHis: 0.304 ± 0.144
1.064HisIle: 1.064 ± 0.298
0.152HisLys: 0.152 ± 0.124
1.823HisLeu: 1.823 ± 0.425
0.456HisMet: 0.456 ± 0.193
0.228HisAsn: 0.228 ± 0.13
1.52HisPro: 1.52 ± 0.344
0.152HisGln: 0.152 ± 0.114
2.583HisArg: 2.583 ± 0.443
0.608HisSer: 0.608 ± 0.241
1.216HisThr: 1.216 ± 0.325
2.051HisVal: 2.051 ± 0.439
0.38HisTrp: 0.38 ± 0.184
0.38HisTyr: 0.38 ± 0.248
0.0HisXaa: 0.0 ± 0.0
Ile
5.85IleAla: 5.85 ± 0.637
0.152IleCys: 0.152 ± 0.098
5.242IleAsp: 5.242 ± 0.708
2.735IleGlu: 2.735 ± 0.431
0.684IlePhe: 0.684 ± 0.215
3.343IleGly: 3.343 ± 0.489
0.76IleHis: 0.76 ± 0.222
2.051IleIle: 2.051 ± 0.477
1.368IleLys: 1.368 ± 0.296
2.659IleLeu: 2.659 ± 0.487
0.38IleMet: 0.38 ± 0.187
0.608IleAsn: 0.608 ± 0.196
3.191IlePro: 3.191 ± 0.472
0.912IleGln: 0.912 ± 0.233
4.862IleArg: 4.862 ± 0.781
1.747IleSer: 1.747 ± 0.367
3.115IleThr: 3.115 ± 0.488
1.747IleVal: 1.747 ± 0.367
0.76IleTrp: 0.76 ± 0.234
0.608IleTyr: 0.608 ± 0.168
0.0IleXaa: 0.0 ± 0.0
Lys
4.255LysAla: 4.255 ± 0.638
0.076LysCys: 0.076 ± 0.074
1.52LysAsp: 1.52 ± 0.374
0.912LysGlu: 0.912 ± 0.219
0.76LysPhe: 0.76 ± 0.207
1.596LysGly: 1.596 ± 0.422
0.608LysHis: 0.608 ± 0.209
1.899LysIle: 1.899 ± 0.414
1.52LysLys: 1.52 ± 0.526
3.571LysLeu: 3.571 ± 0.555
0.304LysMet: 0.304 ± 0.143
0.684LysAsn: 0.684 ± 0.21
2.279LysPro: 2.279 ± 0.467
1.368LysGln: 1.368 ± 0.31
2.963LysArg: 2.963 ± 0.635
1.368LysSer: 1.368 ± 0.303
2.127LysThr: 2.127 ± 0.414
1.671LysVal: 1.671 ± 0.299
0.608LysTrp: 0.608 ± 0.23
1.216LysTyr: 1.216 ± 0.336
0.0LysXaa: 0.0 ± 0.0
Leu
11.017LeuAla: 11.017 ± 1.17
0.228LeuCys: 0.228 ± 0.119
6.838LeuAsp: 6.838 ± 0.906
6.23LeuGlu: 6.23 ± 0.655
1.444LeuPhe: 1.444 ± 0.345
7.446LeuGly: 7.446 ± 0.783
1.975LeuHis: 1.975 ± 0.5
3.799LeuIle: 3.799 ± 0.621
2.051LeuLys: 2.051 ± 0.419
6.002LeuLeu: 6.002 ± 0.829
1.823LeuMet: 1.823 ± 0.365
2.051LeuAsn: 2.051 ± 0.54
4.103LeuPro: 4.103 ± 0.668
2.659LeuGln: 2.659 ± 0.499
6.23LeuArg: 6.23 ± 0.673
3.951LeuSer: 3.951 ± 0.472
4.483LeuThr: 4.483 ± 0.646
5.166LeuVal: 5.166 ± 0.585
1.216LeuTrp: 1.216 ± 0.324
0.608LeuTyr: 0.608 ± 0.235
0.0LeuXaa: 0.0 ± 0.0
Met
3.191MetAla: 3.191 ± 0.499
0.152MetCys: 0.152 ± 0.109
1.064MetAsp: 1.064 ± 0.239
0.836MetGlu: 0.836 ± 0.269
0.836MetPhe: 0.836 ± 0.278
1.747MetGly: 1.747 ± 0.351
0.228MetHis: 0.228 ± 0.128
0.912MetIle: 0.912 ± 0.286
0.912MetLys: 0.912 ± 0.233
1.975MetLeu: 1.975 ± 0.325
1.216MetMet: 1.216 ± 0.281
1.064MetAsn: 1.064 ± 0.288
1.671MetPro: 1.671 ± 0.354
0.456MetGln: 0.456 ± 0.213
2.127MetArg: 2.127 ± 0.402
1.671MetSer: 1.671 ± 0.297
2.583MetThr: 2.583 ± 0.422
1.899MetVal: 1.899 ± 0.497
0.228MetTrp: 0.228 ± 0.146
0.304MetTyr: 0.304 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
3.495AsnAla: 3.495 ± 0.795
0.152AsnCys: 0.152 ± 0.126
1.899AsnAsp: 1.899 ± 0.322
1.368AsnGlu: 1.368 ± 0.337
0.608AsnPhe: 0.608 ± 0.182
2.203AsnGly: 2.203 ± 0.396
0.304AsnHis: 0.304 ± 0.124
0.988AsnIle: 0.988 ± 0.267
0.76AsnLys: 0.76 ± 0.256
2.355AsnLeu: 2.355 ± 0.379
0.532AsnMet: 0.532 ± 0.197
0.608AsnAsn: 0.608 ± 0.198
2.051AsnPro: 2.051 ± 0.413
0.76AsnGln: 0.76 ± 0.282
2.203AsnArg: 2.203 ± 0.446
0.608AsnSer: 0.608 ± 0.202
1.368AsnThr: 1.368 ± 0.32
1.52AsnVal: 1.52 ± 0.345
0.608AsnTrp: 0.608 ± 0.201
0.532AsnTyr: 0.532 ± 0.196
0.0AsnXaa: 0.0 ± 0.0
Pro
8.205ProAla: 8.205 ± 0.929
0.228ProCys: 0.228 ± 0.126
4.787ProAsp: 4.787 ± 0.669
4.103ProGlu: 4.103 ± 0.711
1.368ProPhe: 1.368 ± 0.343
5.09ProGly: 5.09 ± 0.643
1.52ProHis: 1.52 ± 0.416
3.191ProIle: 3.191 ± 0.365
2.127ProLys: 2.127 ± 0.43
4.027ProLeu: 4.027 ± 0.648
1.14ProMet: 1.14 ± 0.365
1.14ProAsn: 1.14 ± 0.352
4.711ProPro: 4.711 ± 1.047
2.583ProGln: 2.583 ± 0.453
4.787ProArg: 4.787 ± 0.734
3.723ProSer: 3.723 ± 0.58
3.571ProThr: 3.571 ± 0.569
4.483ProVal: 4.483 ± 0.839
1.368ProTrp: 1.368 ± 0.314
1.14ProTyr: 1.14 ± 0.329
0.0ProXaa: 0.0 ± 0.0
Gln
3.875GlnAla: 3.875 ± 0.593
0.152GlnCys: 0.152 ± 0.124
1.52GlnAsp: 1.52 ± 0.449
1.975GlnGlu: 1.975 ± 0.363
0.684GlnPhe: 0.684 ± 0.228
2.127GlnGly: 2.127 ± 0.434
0.836GlnHis: 0.836 ± 0.296
1.52GlnIle: 1.52 ± 0.353
1.14GlnLys: 1.14 ± 0.304
4.787GlnLeu: 4.787 ± 0.49
1.216GlnMet: 1.216 ± 0.284
0.304GlnAsn: 0.304 ± 0.158
2.127GlnPro: 2.127 ± 0.523
1.292GlnGln: 1.292 ± 0.382
2.887GlnArg: 2.887 ± 0.37
1.444GlnSer: 1.444 ± 0.296
1.975GlnThr: 1.975 ± 0.423
1.444GlnVal: 1.444 ± 0.298
0.988GlnTrp: 0.988 ± 0.218
0.684GlnTyr: 0.684 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
9.725ArgAla: 9.725 ± 0.815
0.76ArgCys: 0.76 ± 0.329
4.787ArgAsp: 4.787 ± 0.618
5.242ArgGlu: 5.242 ± 0.654
1.596ArgPhe: 1.596 ± 0.342
5.318ArgGly: 5.318 ± 0.695
1.899ArgHis: 1.899 ± 0.413
3.495ArgIle: 3.495 ± 0.654
3.115ArgLys: 3.115 ± 0.609
6.306ArgLeu: 6.306 ± 0.685
2.887ArgMet: 2.887 ± 0.462
2.355ArgAsn: 2.355 ± 0.477
4.938ArgPro: 4.938 ± 0.681
3.571ArgGln: 3.571 ± 0.506
9.649ArgArg: 9.649 ± 1.443
3.419ArgSer: 3.419 ± 0.572
3.495ArgThr: 3.495 ± 0.638
5.318ArgVal: 5.318 ± 0.512
2.279ArgTrp: 2.279 ± 0.435
2.127ArgTyr: 2.127 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
5.318SerAla: 5.318 ± 0.746
0.228SerCys: 0.228 ± 0.146
2.887SerAsp: 2.887 ± 0.452
2.279SerGlu: 2.279 ± 0.463
1.292SerPhe: 1.292 ± 0.345
5.926SerGly: 5.926 ± 0.83
0.304SerHis: 0.304 ± 0.118
1.975SerIle: 1.975 ± 0.435
1.368SerLys: 1.368 ± 0.414
3.191SerLeu: 3.191 ± 0.521
1.899SerMet: 1.899 ± 0.405
1.444SerAsn: 1.444 ± 0.321
3.875SerPro: 3.875 ± 0.599
1.747SerGln: 1.747 ± 0.34
3.647SerArg: 3.647 ± 0.653
3.039SerSer: 3.039 ± 0.576
3.191SerThr: 3.191 ± 0.489
3.191SerVal: 3.191 ± 0.359
0.76SerTrp: 0.76 ± 0.244
1.064SerTyr: 1.064 ± 0.271
0.0SerXaa: 0.0 ± 0.0
Thr
8.813ThrAla: 8.813 ± 0.895
0.304ThrCys: 0.304 ± 0.203
4.027ThrAsp: 4.027 ± 0.471
4.331ThrGlu: 4.331 ± 0.531
1.671ThrPhe: 1.671 ± 0.298
4.938ThrGly: 4.938 ± 0.967
0.988ThrHis: 0.988 ± 0.291
2.811ThrIle: 2.811 ± 0.42
1.596ThrLys: 1.596 ± 0.316
5.242ThrLeu: 5.242 ± 0.776
1.292ThrMet: 1.292 ± 0.291
1.596ThrAsn: 1.596 ± 0.308
4.331ThrPro: 4.331 ± 0.652
1.444ThrGln: 1.444 ± 0.357
4.787ThrArg: 4.787 ± 0.641
3.115ThrSer: 3.115 ± 0.492
4.635ThrThr: 4.635 ± 0.828
5.242ThrVal: 5.242 ± 0.826
1.368ThrTrp: 1.368 ± 0.338
1.14ThrTyr: 1.14 ± 0.247
0.0ThrXaa: 0.0 ± 0.0
Val
9.269ValAla: 9.269 ± 0.794
0.608ValCys: 0.608 ± 0.224
5.09ValAsp: 5.09 ± 0.661
3.875ValGlu: 3.875 ± 0.555
1.747ValPhe: 1.747 ± 0.4
5.546ValGly: 5.546 ± 0.626
1.064ValHis: 1.064 ± 0.231
2.963ValIle: 2.963 ± 0.609
1.899ValLys: 1.899 ± 0.383
5.622ValLeu: 5.622 ± 0.646
1.596ValMet: 1.596 ± 0.319
1.671ValAsn: 1.671 ± 0.333
4.027ValPro: 4.027 ± 0.494
1.747ValGln: 1.747 ± 0.362
5.09ValArg: 5.09 ± 0.589
3.267ValSer: 3.267 ± 0.686
4.862ValThr: 4.862 ± 0.739
5.698ValVal: 5.698 ± 0.734
0.684ValTrp: 0.684 ± 0.238
1.975ValTyr: 1.975 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
2.735TrpAla: 2.735 ± 0.468
0.38TrpCys: 0.38 ± 0.153
0.836TrpAsp: 0.836 ± 0.212
1.596TrpGlu: 1.596 ± 0.407
0.532TrpPhe: 0.532 ± 0.207
1.14TrpGly: 1.14 ± 0.334
0.532TrpHis: 0.532 ± 0.209
0.988TrpIle: 0.988 ± 0.315
0.152TrpLys: 0.152 ± 0.098
1.368TrpLeu: 1.368 ± 0.271
0.304TrpMet: 0.304 ± 0.166
0.608TrpAsn: 0.608 ± 0.189
1.444TrpPro: 1.444 ± 0.305
0.912TrpGln: 0.912 ± 0.237
1.975TrpArg: 1.975 ± 0.413
0.988TrpSer: 0.988 ± 0.279
1.064TrpThr: 1.064 ± 0.311
1.292TrpVal: 1.292 ± 0.418
0.532TrpTrp: 0.532 ± 0.191
0.532TrpTyr: 0.532 ± 0.258
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.963TyrAla: 2.963 ± 0.399
0.152TyrCys: 0.152 ± 0.1
1.064TyrAsp: 1.064 ± 0.297
1.671TyrGlu: 1.671 ± 0.292
0.608TyrPhe: 0.608 ± 0.216
2.279TyrGly: 2.279 ± 0.411
0.608TyrHis: 0.608 ± 0.219
0.152TyrIle: 0.152 ± 0.097
0.532TyrLys: 0.532 ± 0.168
1.292TyrLeu: 1.292 ± 0.358
0.304TyrMet: 0.304 ± 0.149
0.228TyrAsn: 0.228 ± 0.129
1.216TyrPro: 1.216 ± 0.305
0.988TyrGln: 0.988 ± 0.271
1.368TyrArg: 1.368 ± 0.35
1.216TyrSer: 1.216 ± 0.322
1.216TyrThr: 1.216 ± 0.298
1.52TyrVal: 1.52 ± 0.325
0.38TyrTrp: 0.38 ± 0.142
0.456TyrTyr: 0.456 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13163 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski