Tardiphaga robiniae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Tardiphaga

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A164ART6|A0A164ART6_9BRAD Corrinoid adenosyltransferase OS=Tardiphaga robiniae OX=943830 GN=A4A58_01690 PE=3 SV=1
MM1 pKa = 7.35SLSGALSSAISALNAQSSALAMISDD26 pKa = 5.05NIANSSTVGYY36 pKa = 7.67KK37 pKa = 7.36TTSASFEE44 pKa = 4.42SLVTSSSSASSYY56 pKa = 11.11SSGGVSTLSKK66 pKa = 11.28SNITTQGLLTATSTSTNIAIQGNGFFPVTTSLTGGSTLYY105 pKa = 9.85TRR107 pKa = 11.84NGAFTTDD114 pKa = 2.73SDD116 pKa = 4.61GYY118 pKa = 10.73LVNNGAYY125 pKa = 10.56LLGWRR130 pKa = 11.84TDD132 pKa = 2.87ASGNVIGSASSGNLTAIDD150 pKa = 3.53TSVAQTSGSATTTTTFSANLPADD173 pKa = 3.68AAVGDD178 pKa = 4.15TFTTSMSAYY187 pKa = 9.86DD188 pKa = 3.61SLGTSSSIQVTWSKK202 pKa = 9.2TADD205 pKa = 3.79NTWSASFGQPTLSSDD220 pKa = 3.73STVTTAATPTSTVAVTFNTDD240 pKa = 2.38GTLASTTPDD249 pKa = 3.67PPVLALTGSTTGAADD264 pKa = 3.37NTITLNLGTVGGTDD278 pKa = 3.53GLTQYY283 pKa = 11.65ASGLSTPAVNITRR296 pKa = 11.84IEE298 pKa = 4.41SNGLAFGKK306 pKa = 10.25LSNISVGDD314 pKa = 3.65NGVVNATYY322 pKa = 11.24SNGQTIAIYY331 pKa = 9.49KK332 pKa = 10.05IPVATFAAADD342 pKa = 3.57QLEE345 pKa = 4.34AQSNGLYY352 pKa = 9.21STTAEE357 pKa = 4.3SGTAALQEE365 pKa = 4.55SGTNGAGTIYY375 pKa = 10.5GSEE378 pKa = 4.49LEE380 pKa = 4.75SSTTDD385 pKa = 3.06TNEE388 pKa = 3.87QFSSMISAQQAYY400 pKa = 9.56SAASQVITAVNKK412 pKa = 9.85MFDD415 pKa = 3.61TLISSMRR422 pKa = 3.49

Molecular weight: 42.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A163ZA08|A0A163ZA08_9BRAD Class II aldolase OS=Tardiphaga robiniae OX=943830 GN=A4A58_07385 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5721

0

5721

1807327

26

5746

315.9

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.217 ± 0.038

0.78 ± 0.011

5.666 ± 0.032

4.989 ± 0.035

3.8 ± 0.021

8.467 ± 0.049

1.924 ± 0.019

5.658 ± 0.023

3.872 ± 0.03

9.766 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.018

2.933 ± 0.026

5.073 ± 0.026

3.185 ± 0.018

6.401 ± 0.037

5.79 ± 0.028

5.705 ± 0.037

7.611 ± 0.027

1.269 ± 0.012

2.289 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski