Halorubrum sp. CBA1125

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; unclassified Halorubrum

Average proteome isoelectric point is 4.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A9T8R6|A0A6A9T8R6_9EURY Uncharacterized protein OS=Halorubrum sp. CBA1125 OX=2668072 GN=GJ633_08520 PE=4 SV=1
MM1 pKa = 7.6GLTDD5 pKa = 3.52TLAARR10 pKa = 11.84FDD12 pKa = 4.07VAEE15 pKa = 4.73HH16 pKa = 6.37GSDD19 pKa = 3.11VRR21 pKa = 11.84TEE23 pKa = 4.09VVAGITTFLAMSYY36 pKa = 9.92IIVVNPTILSNAIQIEE52 pKa = 5.14GYY54 pKa = 9.23SQGEE58 pKa = 4.73VIQMIAIATVLSSAVATLVMAFYY81 pKa = 11.22ANRR84 pKa = 11.84PFGLAPGLGLNAFFAFTVVLGLGIPWEE111 pKa = 4.16TALAAVFVEE120 pKa = 4.94GVLFMVLTAIGARR133 pKa = 11.84EE134 pKa = 3.97YY135 pKa = 10.26IIQLFPEE142 pKa = 4.3PVKK145 pKa = 10.61RR146 pKa = 11.84SVGAGIGLFLLFIGFQEE163 pKa = 4.06LQIIVNDD170 pKa = 3.38EE171 pKa = 4.05ATFVTLGQIFANPWAILGVLGLVLTFVLWARR202 pKa = 11.84DD203 pKa = 3.22ITGAIVIGILTTAAAGWALTLGGVFDD229 pKa = 5.35RR230 pKa = 11.84GTITPEE236 pKa = 3.79SLPAAQYY243 pKa = 10.51DD244 pKa = 3.53ISPLVGAFVEE254 pKa = 4.82GLADD258 pKa = 3.7VDD260 pKa = 4.21PLTFTLVVFTFFFVDD275 pKa = 4.8FFDD278 pKa = 3.96TAGTLIGVSQFGDD291 pKa = 3.54FLDD294 pKa = 5.43DD295 pKa = 4.54EE296 pKa = 5.5GDD298 pKa = 3.73LPEE301 pKa = 4.25MDD303 pKa = 4.4RR304 pKa = 11.84PLMADD309 pKa = 2.98AVGTTFGAIVGTSTVTTYY327 pKa = 10.34IEE329 pKa = 4.18SSTGVEE335 pKa = 3.77EE336 pKa = 4.63GGRR339 pKa = 11.84TGLTALVVAIAFLASLALIPVVAAIPAYY367 pKa = 10.36ASFIALIVVGVIMLQGLVEE386 pKa = 4.34VDD388 pKa = 2.88WTDD391 pKa = 3.42PAWAVSAGLTVTVMPLAYY409 pKa = 10.38SIADD413 pKa = 3.53GLAAGIVAYY422 pKa = 8.68PIIKK426 pKa = 9.77VAIGEE431 pKa = 4.24YY432 pKa = 10.53DD433 pKa = 3.63DD434 pKa = 4.58VAAGQYY440 pKa = 10.29VIAAVLAAYY449 pKa = 9.77YY450 pKa = 10.07VFQTSGLILL459 pKa = 3.89

Molecular weight: 48.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A9T5C4|A0A6A9T5C4_9EURY Phosphoesterase OS=Halorubrum sp. CBA1125 OX=2668072 GN=GJ633_06600 PE=3 SV=1
MM1 pKa = 7.22TVRR4 pKa = 11.84KK5 pKa = 9.12AAFSPAVPSAVVAVALAAGVVVSLDD30 pKa = 3.11ARR32 pKa = 11.84EE33 pKa = 4.18AVLATLAVQGGGLAVVGIGVASRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84GHH60 pKa = 6.99RR61 pKa = 11.84ILGTALAVVGAGVIVGAFDD80 pKa = 4.52LFVDD84 pKa = 3.89EE85 pKa = 5.12RR86 pKa = 11.84HH87 pKa = 5.87ALSYY91 pKa = 9.26TIRR94 pKa = 11.84FLPGLVGIPLLAGTLLPVWASGSRR118 pKa = 11.84LVCKK122 pKa = 10.43LGTGGVFLTVVFSGLFSAVGEE143 pKa = 4.05LHH145 pKa = 6.88LLLAAVATVVAWDD158 pKa = 3.61AADD161 pKa = 3.48NAVGIGEE168 pKa = 4.3HH169 pKa = 6.75LGRR172 pKa = 11.84AATSWRR178 pKa = 11.84LQVTHH183 pKa = 5.7TTGSAAVGLAGVAGIHH199 pKa = 6.01LVRR202 pKa = 11.84HH203 pKa = 5.33VAVSDD208 pKa = 3.94LSLAAFALLFVALLLLLAAIRR229 pKa = 11.84GG230 pKa = 3.82

Molecular weight: 23.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2859

0

2859

754458

15

3720

263.9

28.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.074 ± 0.074

0.71 ± 0.016

8.769 ± 0.063

8.419 ± 0.064

3.283 ± 0.03

8.534 ± 0.058

1.949 ± 0.026

4.181 ± 0.035

1.787 ± 0.032

8.613 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.658 ± 0.025

2.372 ± 0.04

4.729 ± 0.034

2.354 ± 0.028

6.716 ± 0.06

5.395 ± 0.048

6.445 ± 0.069

9.216 ± 0.055

1.115 ± 0.018

2.681 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski