Tissierellia bacterium S5-A11

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; unclassified Tissierellia

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2755 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095XP77|A0A095XP77_9FIRM Uncharacterized protein OS=Tissierellia bacterium S5-A11 OX=1230730 GN=HMPREF1633_05780 PE=4 SV=1
MM1 pKa = 7.45ANIAIVYY8 pKa = 6.07WTGSGNTEE16 pKa = 3.59AMANAIDD23 pKa = 4.34EE24 pKa = 4.46GAKK27 pKa = 10.34AAGAEE32 pKa = 4.3TQLSFVTDD40 pKa = 3.28VTADD44 pKa = 4.06DD45 pKa = 4.07IAAFDD50 pKa = 4.69YY51 pKa = 10.9IALGCPAMGSEE62 pKa = 4.19QLEE65 pKa = 4.42EE66 pKa = 4.17YY67 pKa = 10.61DD68 pKa = 4.47FEE70 pKa = 4.53PFYY73 pKa = 11.48EE74 pKa = 4.23EE75 pKa = 4.98LAGHH79 pKa = 7.09LAGKK83 pKa = 9.78KK84 pKa = 8.81VALFGSYY91 pKa = 8.88AWNDD95 pKa = 4.11GQWMDD100 pKa = 3.46DD101 pKa = 2.95WAGRR105 pKa = 11.84VAEE108 pKa = 4.34TGADD112 pKa = 3.1IVADD116 pKa = 3.8PVKK119 pKa = 10.57AYY121 pKa = 10.11AYY123 pKa = 9.44PDD125 pKa = 4.03DD126 pKa = 5.49DD127 pKa = 4.49ALEE130 pKa = 4.36ACRR133 pKa = 11.84QLGAALANAA142 pKa = 4.74

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095XRS0|A0A095XRS0_9FIRM 5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Tissierellia bacterium S5-A11 OX=1230730 GN=mtaD PE=3 SV=1
MM1 pKa = 7.61INYY4 pKa = 7.56LLCVMGAYY12 pKa = 10.7LLGSIPSGLWLGKK25 pKa = 9.37LLCGTDD31 pKa = 3.6LRR33 pKa = 11.84RR34 pKa = 11.84HH35 pKa = 5.72GSRR38 pKa = 11.84NIGATNAYY46 pKa = 6.86RR47 pKa = 11.84TLGARR52 pKa = 11.84MGIAVFAADD61 pKa = 3.81FLKK64 pKa = 10.9GVAATSLGGNDD75 pKa = 3.45PVLLAFCAAAAIVGHH90 pKa = 6.35CFSVFLGFRR99 pKa = 11.84GGRR102 pKa = 11.84GVATGFGVMCCLSLPVAVAAAVVWAVLVFATRR134 pKa = 11.84LVSLASIVAALTVPILLWWWQAPSAFLIFATLGVIAIIFQHH175 pKa = 6.44RR176 pKa = 11.84ANIGRR181 pKa = 11.84LLRR184 pKa = 11.84GEE186 pKa = 4.16EE187 pKa = 4.01KK188 pKa = 10.52QIEE191 pKa = 4.16RR192 pKa = 11.84AKK194 pKa = 10.48LKK196 pKa = 10.38RR197 pKa = 11.84RR198 pKa = 11.84RR199 pKa = 11.84KK200 pKa = 7.78TKK202 pKa = 10.44

Molecular weight: 21.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2755

0

2755

864364

35

2592

313.7

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.942 ± 0.082

0.927 ± 0.014

5.948 ± 0.039

7.026 ± 0.051

4.041 ± 0.037

7.138 ± 0.043

1.869 ± 0.02

7.073 ± 0.043

6.924 ± 0.074

10.285 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.025

3.911 ± 0.041

3.631 ± 0.03

3.859 ± 0.035

4.81 ± 0.044

5.413 ± 0.039

5.1 ± 0.039

6.847 ± 0.042

0.863 ± 0.015

3.727 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski