Amino acid dipepetide frequency for Mycobacterium phage Jabbawokkie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.669AlaAla: 14.669 ± 1.868
1.241AlaCys: 1.241 ± 0.304
5.924AlaAsp: 5.924 ± 0.536
8.294AlaGlu: 8.294 ± 0.83
3.103AlaPhe: 3.103 ± 0.384
8.745AlaGly: 8.745 ± 1.23
2.313AlaHis: 2.313 ± 0.36
4.852AlaIle: 4.852 ± 0.613
4.344AlaLys: 4.344 ± 0.472
9.986AlaLeu: 9.986 ± 0.874
3.16AlaMet: 3.16 ± 0.45
3.498AlaAsn: 3.498 ± 0.521
4.457AlaPro: 4.457 ± 0.528
4.119AlaGln: 4.119 ± 0.544
6.658AlaArg: 6.658 ± 0.871
4.57AlaSer: 4.57 ± 0.529
5.36AlaThr: 5.36 ± 0.585
8.181AlaVal: 8.181 ± 0.689
2.595AlaTrp: 2.595 ± 0.471
1.975AlaTyr: 1.975 ± 0.333
0.0AlaXaa: 0.0 ± 0.0
Cys
1.411CysAla: 1.411 ± 0.342
0.169CysCys: 0.169 ± 0.104
1.016CysAsp: 1.016 ± 0.323
1.072CysGlu: 1.072 ± 0.273
0.226CysPhe: 0.226 ± 0.121
1.805CysGly: 1.805 ± 0.382
0.339CysHis: 0.339 ± 0.139
0.339CysIle: 0.339 ± 0.152
0.395CysLys: 0.395 ± 0.142
0.677CysLeu: 0.677 ± 0.241
0.113CysMet: 0.113 ± 0.067
0.508CysAsn: 0.508 ± 0.178
0.959CysPro: 0.959 ± 0.228
0.451CysGln: 0.451 ± 0.194
1.128CysArg: 1.128 ± 0.326
1.185CysSer: 1.185 ± 0.287
0.621CysThr: 0.621 ± 0.202
0.677CysVal: 0.677 ± 0.193
0.339CysTrp: 0.339 ± 0.158
0.282CysTyr: 0.282 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
7.053AspAla: 7.053 ± 0.621
0.733AspCys: 0.733 ± 0.21
4.457AspAsp: 4.457 ± 0.582
4.626AspGlu: 4.626 ± 0.636
2.257AspPhe: 2.257 ± 0.371
5.473AspGly: 5.473 ± 0.563
1.411AspHis: 1.411 ± 0.295
3.442AspIle: 3.442 ± 0.497
1.862AspLys: 1.862 ± 0.363
4.57AspLeu: 4.57 ± 0.518
1.411AspMet: 1.411 ± 0.322
1.749AspAsn: 1.749 ± 0.386
4.119AspPro: 4.119 ± 0.526
2.2AspGln: 2.2 ± 0.358
4.457AspArg: 4.457 ± 0.521
3.103AspSer: 3.103 ± 0.416
3.329AspThr: 3.329 ± 0.447
4.401AspVal: 4.401 ± 0.474
1.523AspTrp: 1.523 ± 0.285
1.298AspTyr: 1.298 ± 0.323
0.0AspXaa: 0.0 ± 0.0
Glu
6.488GluAla: 6.488 ± 0.681
1.467GluCys: 1.467 ± 0.343
4.232GluAsp: 4.232 ± 0.598
3.498GluGlu: 3.498 ± 0.64
2.37GluPhe: 2.37 ± 0.342
3.837GluGly: 3.837 ± 0.643
1.467GluHis: 1.467 ± 0.308
2.257GluIle: 2.257 ± 0.387
2.144GluLys: 2.144 ± 0.365
5.698GluLeu: 5.698 ± 0.494
1.241GluMet: 1.241 ± 0.235
1.749GluAsn: 1.749 ± 0.314
3.555GluPro: 3.555 ± 0.486
2.539GluGln: 2.539 ± 0.335
3.893GluArg: 3.893 ± 0.677
3.611GluSer: 3.611 ± 0.477
3.272GluThr: 3.272 ± 0.493
4.909GluVal: 4.909 ± 0.542
1.354GluTrp: 1.354 ± 0.22
1.523GluTyr: 1.523 ± 0.352
0.0GluXaa: 0.0 ± 0.0
Phe
2.877PheAla: 2.877 ± 0.358
0.451PheCys: 0.451 ± 0.152
2.426PheAsp: 2.426 ± 0.405
1.918PheGlu: 1.918 ± 0.385
0.508PhePhe: 0.508 ± 0.137
2.652PheGly: 2.652 ± 0.376
0.564PheHis: 0.564 ± 0.165
1.298PheIle: 1.298 ± 0.272
1.185PheLys: 1.185 ± 0.302
2.313PheLeu: 2.313 ± 0.404
0.903PheMet: 0.903 ± 0.202
0.733PheAsn: 0.733 ± 0.183
1.411PhePro: 1.411 ± 0.227
0.733PheGln: 0.733 ± 0.215
1.523PheArg: 1.523 ± 0.299
2.031PheSer: 2.031 ± 0.305
2.144PheThr: 2.144 ± 0.386
1.918PheVal: 1.918 ± 0.309
0.621PheTrp: 0.621 ± 0.165
0.508PheTyr: 0.508 ± 0.158
0.0PheXaa: 0.0 ± 0.0
Gly
9.084GlyAla: 9.084 ± 1.529
0.846GlyCys: 0.846 ± 0.247
4.909GlyAsp: 4.909 ± 0.566
4.062GlyGlu: 4.062 ± 0.438
2.144GlyPhe: 2.144 ± 0.361
11.002GlyGly: 11.002 ± 2.695
1.749GlyHis: 1.749 ± 0.313
3.329GlyIle: 3.329 ± 0.498
3.329GlyLys: 3.329 ± 0.48
5.868GlyLeu: 5.868 ± 0.753
1.58GlyMet: 1.58 ± 0.256
2.539GlyAsn: 2.539 ± 0.394
3.837GlyPro: 3.837 ± 0.475
3.78GlyGln: 3.78 ± 0.584
5.304GlyArg: 5.304 ± 0.496
5.755GlySer: 5.755 ± 1.002
6.545GlyThr: 6.545 ± 0.645
6.714GlyVal: 6.714 ± 0.62
2.426GlyTrp: 2.426 ± 0.365
2.2GlyTyr: 2.2 ± 0.333
0.0GlyXaa: 0.0 ± 0.0
His
1.411HisAla: 1.411 ± 0.27
0.395HisCys: 0.395 ± 0.136
1.354HisAsp: 1.354 ± 0.291
0.903HisGlu: 0.903 ± 0.206
0.282HisPhe: 0.282 ± 0.128
1.636HisGly: 1.636 ± 0.254
0.959HisHis: 0.959 ± 0.242
1.128HisIle: 1.128 ± 0.262
0.959HisLys: 0.959 ± 0.229
1.862HisLeu: 1.862 ± 0.416
0.508HisMet: 0.508 ± 0.141
0.733HisAsn: 0.733 ± 0.19
1.523HisPro: 1.523 ± 0.244
0.564HisGln: 0.564 ± 0.197
2.088HisArg: 2.088 ± 0.421
1.072HisSer: 1.072 ± 0.191
1.128HisThr: 1.128 ± 0.283
1.467HisVal: 1.467 ± 0.34
0.621HisTrp: 0.621 ± 0.174
0.677HisTyr: 0.677 ± 0.245
0.0HisXaa: 0.0 ± 0.0
Ile
5.304IleAla: 5.304 ± 0.621
0.621IleCys: 0.621 ± 0.206
3.837IleAsp: 3.837 ± 0.495
3.385IleGlu: 3.385 ± 0.418
0.79IlePhe: 0.79 ± 0.201
4.232IleGly: 4.232 ± 0.575
1.298IleHis: 1.298 ± 0.276
1.805IleIle: 1.805 ± 0.337
1.298IleLys: 1.298 ± 0.278
1.805IleLeu: 1.805 ± 0.405
0.677IleMet: 0.677 ± 0.224
1.805IleAsn: 1.805 ± 0.369
2.483IlePro: 2.483 ± 0.4
1.467IleGln: 1.467 ± 0.336
3.442IleArg: 3.442 ± 0.414
2.144IleSer: 2.144 ± 0.473
4.006IleThr: 4.006 ± 0.543
2.652IleVal: 2.652 ± 0.312
0.79IleTrp: 0.79 ± 0.218
0.959IleTyr: 0.959 ± 0.211
0.0IleXaa: 0.0 ± 0.0
Lys
4.232LysAla: 4.232 ± 0.635
0.282LysCys: 0.282 ± 0.119
2.2LysAsp: 2.2 ± 0.35
1.58LysGlu: 1.58 ± 0.374
0.846LysPhe: 0.846 ± 0.22
2.37LysGly: 2.37 ± 0.391
1.128LysHis: 1.128 ± 0.29
1.975LysIle: 1.975 ± 0.295
1.298LysLys: 1.298 ± 0.334
2.821LysLeu: 2.821 ± 0.355
0.564LysMet: 0.564 ± 0.143
0.903LysAsn: 0.903 ± 0.307
2.821LysPro: 2.821 ± 0.353
1.805LysGln: 1.805 ± 0.399
3.16LysArg: 3.16 ± 0.444
2.144LysSer: 2.144 ± 0.384
2.088LysThr: 2.088 ± 0.379
2.088LysVal: 2.088 ± 0.319
1.016LysTrp: 1.016 ± 0.21
0.903LysTyr: 0.903 ± 0.246
0.0LysXaa: 0.0 ± 0.0
Leu
9.253LeuAla: 9.253 ± 0.95
0.959LeuCys: 0.959 ± 0.298
4.909LeuAsp: 4.909 ± 0.587
3.272LeuGlu: 3.272 ± 0.351
2.088LeuPhe: 2.088 ± 0.295
6.206LeuGly: 6.206 ± 0.629
1.128LeuHis: 1.128 ± 0.277
4.006LeuIle: 4.006 ± 0.515
3.329LeuLys: 3.329 ± 0.378
5.811LeuLeu: 5.811 ± 0.79
1.298LeuMet: 1.298 ± 0.282
2.37LeuAsn: 2.37 ± 0.365
5.416LeuPro: 5.416 ± 0.516
2.934LeuGln: 2.934 ± 0.478
4.909LeuArg: 4.909 ± 0.579
5.868LeuSer: 5.868 ± 0.639
5.473LeuThr: 5.473 ± 0.586
4.909LeuVal: 4.909 ± 0.619
1.354LeuTrp: 1.354 ± 0.344
1.862LeuTyr: 1.862 ± 0.277
0.0LeuXaa: 0.0 ± 0.0
Met
2.652MetAla: 2.652 ± 0.408
0.395MetCys: 0.395 ± 0.226
1.185MetAsp: 1.185 ± 0.252
0.903MetGlu: 0.903 ± 0.171
0.677MetPhe: 0.677 ± 0.286
1.523MetGly: 1.523 ± 0.352
0.282MetHis: 0.282 ± 0.128
1.241MetIle: 1.241 ± 0.267
0.846MetLys: 0.846 ± 0.273
1.749MetLeu: 1.749 ± 0.276
0.451MetMet: 0.451 ± 0.152
0.846MetAsn: 0.846 ± 0.214
1.241MetPro: 1.241 ± 0.215
0.169MetGln: 0.169 ± 0.097
1.241MetArg: 1.241 ± 0.205
2.539MetSer: 2.539 ± 0.38
1.862MetThr: 1.862 ± 0.319
1.185MetVal: 1.185 ± 0.214
0.395MetTrp: 0.395 ± 0.177
0.226MetTyr: 0.226 ± 0.106
0.0MetXaa: 0.0 ± 0.0
Asn
3.047AsnAla: 3.047 ± 0.479
0.508AsnCys: 0.508 ± 0.172
1.467AsnAsp: 1.467 ± 0.238
1.749AsnGlu: 1.749 ± 0.295
0.677AsnPhe: 0.677 ± 0.188
3.893AsnGly: 3.893 ± 0.542
0.564AsnHis: 0.564 ± 0.206
1.016AsnIle: 1.016 ± 0.241
0.903AsnLys: 0.903 ± 0.181
2.821AsnLeu: 2.821 ± 0.382
0.621AsnMet: 0.621 ± 0.173
0.959AsnAsn: 0.959 ± 0.198
2.483AsnPro: 2.483 ± 0.41
0.564AsnGln: 0.564 ± 0.208
1.749AsnArg: 1.749 ± 0.36
1.862AsnSer: 1.862 ± 0.335
2.313AsnThr: 2.313 ± 0.36
1.918AsnVal: 1.918 ± 0.312
0.677AsnTrp: 0.677 ± 0.157
0.903AsnTyr: 0.903 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
5.473ProAla: 5.473 ± 0.55
1.016ProCys: 1.016 ± 0.272
4.739ProAsp: 4.739 ± 0.581
4.739ProGlu: 4.739 ± 0.525
2.257ProPhe: 2.257 ± 0.307
5.811ProGly: 5.811 ± 0.69
1.016ProHis: 1.016 ± 0.21
1.749ProIle: 1.749 ± 0.322
2.144ProLys: 2.144 ± 0.334
3.498ProLeu: 3.498 ± 0.439
1.241ProMet: 1.241 ± 0.292
2.2ProAsn: 2.2 ± 0.409
3.555ProPro: 3.555 ± 0.774
1.636ProGln: 1.636 ± 0.249
3.78ProArg: 3.78 ± 0.46
2.652ProSer: 2.652 ± 0.464
4.175ProThr: 4.175 ± 0.56
3.837ProVal: 3.837 ± 0.484
1.128ProTrp: 1.128 ± 0.269
1.467ProTyr: 1.467 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
4.175GlnAla: 4.175 ± 0.572
0.395GlnCys: 0.395 ± 0.194
1.636GlnAsp: 1.636 ± 0.301
1.693GlnGlu: 1.693 ± 0.279
1.016GlnPhe: 1.016 ± 0.224
2.539GlnGly: 2.539 ± 0.452
1.016GlnHis: 1.016 ± 0.212
1.862GlnIle: 1.862 ± 0.307
2.031GlnLys: 2.031 ± 0.435
2.99GlnLeu: 2.99 ± 0.388
1.016GlnMet: 1.016 ± 0.279
1.016GlnAsn: 1.016 ± 0.278
2.313GlnPro: 2.313 ± 0.445
1.636GlnGln: 1.636 ± 0.366
2.595GlnArg: 2.595 ± 0.425
2.257GlnSer: 2.257 ± 0.328
1.805GlnThr: 1.805 ± 0.325
2.031GlnVal: 2.031 ± 0.355
0.677GlnTrp: 0.677 ± 0.21
1.128GlnTyr: 1.128 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
6.432ArgAla: 6.432 ± 0.669
1.128ArgCys: 1.128 ± 0.332
4.062ArgAsp: 4.062 ± 0.457
4.401ArgGlu: 4.401 ± 0.554
2.426ArgPhe: 2.426 ± 0.411
4.175ArgGly: 4.175 ± 0.333
1.354ArgHis: 1.354 ± 0.302
3.103ArgIle: 3.103 ± 0.49
2.821ArgLys: 2.821 ± 0.495
6.037ArgLeu: 6.037 ± 0.747
1.918ArgMet: 1.918 ± 0.361
1.636ArgAsn: 1.636 ± 0.271
3.78ArgPro: 3.78 ± 0.507
3.16ArgGln: 3.16 ± 0.542
6.545ArgArg: 6.545 ± 1.002
3.949ArgSer: 3.949 ± 0.472
3.16ArgThr: 3.16 ± 0.464
5.191ArgVal: 5.191 ± 0.64
2.313ArgTrp: 2.313 ± 0.305
1.298ArgTyr: 1.298 ± 0.243
0.0ArgXaa: 0.0 ± 0.0
Ser
6.376SerAla: 6.376 ± 0.772
0.564SerCys: 0.564 ± 0.224
3.837SerAsp: 3.837 ± 0.417
3.216SerGlu: 3.216 ± 0.497
1.805SerPhe: 1.805 ± 0.366
6.601SerGly: 6.601 ± 0.833
1.128SerHis: 1.128 ± 0.251
2.595SerIle: 2.595 ± 0.423
2.031SerLys: 2.031 ± 0.383
4.514SerLeu: 4.514 ± 0.518
1.749SerMet: 1.749 ± 0.286
2.257SerAsn: 2.257 ± 0.371
3.329SerPro: 3.329 ± 0.379
2.088SerGln: 2.088 ± 0.382
4.062SerArg: 4.062 ± 0.53
3.329SerSer: 3.329 ± 0.538
3.949SerThr: 3.949 ± 0.413
5.021SerVal: 5.021 ± 0.74
1.185SerTrp: 1.185 ± 0.302
1.241SerTyr: 1.241 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
6.376ThrAla: 6.376 ± 0.763
0.733ThrCys: 0.733 ± 0.201
4.006ThrAsp: 4.006 ± 0.472
3.78ThrGlu: 3.78 ± 0.391
1.467ThrPhe: 1.467 ± 0.319
5.755ThrGly: 5.755 ± 0.785
0.959ThrHis: 0.959 ± 0.217
3.216ThrIle: 3.216 ± 0.526
1.411ThrLys: 1.411 ± 0.259
5.191ThrLeu: 5.191 ± 0.59
0.903ThrMet: 0.903 ± 0.219
2.144ThrAsn: 2.144 ± 0.347
5.529ThrPro: 5.529 ± 0.744
1.749ThrGln: 1.749 ± 0.344
4.175ThrArg: 4.175 ± 0.566
4.232ThrSer: 4.232 ± 0.479
4.232ThrThr: 4.232 ± 0.571
4.796ThrVal: 4.796 ± 0.668
1.693ThrTrp: 1.693 ± 0.351
1.128ThrTyr: 1.128 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
7.899ValAla: 7.899 ± 0.629
0.903ValCys: 0.903 ± 0.217
3.78ValAsp: 3.78 ± 0.544
5.247ValGlu: 5.247 ± 0.536
2.031ValPhe: 2.031 ± 0.332
4.909ValGly: 4.909 ± 0.549
1.805ValHis: 1.805 ± 0.364
3.555ValIle: 3.555 ± 0.518
2.595ValLys: 2.595 ± 0.391
5.134ValLeu: 5.134 ± 0.675
1.072ValMet: 1.072 ± 0.286
1.805ValAsn: 1.805 ± 0.282
3.442ValPro: 3.442 ± 0.404
2.539ValGln: 2.539 ± 0.359
5.021ValArg: 5.021 ± 0.668
5.642ValSer: 5.642 ± 0.869
4.57ValThr: 4.57 ± 0.652
6.94ValVal: 6.94 ± 0.809
1.862ValTrp: 1.862 ± 0.329
1.749ValTyr: 1.749 ± 0.356
0.0ValXaa: 0.0 ± 0.0
Trp
1.805TrpAla: 1.805 ± 0.303
0.508TrpCys: 0.508 ± 0.198
1.693TrpAsp: 1.693 ± 0.306
1.016TrpGlu: 1.016 ± 0.293
0.79TrpPhe: 0.79 ± 0.216
1.467TrpGly: 1.467 ± 0.344
0.339TrpHis: 0.339 ± 0.127
1.411TrpIle: 1.411 ± 0.311
0.959TrpLys: 0.959 ± 0.214
2.426TrpLeu: 2.426 ± 0.353
0.733TrpMet: 0.733 ± 0.2
0.564TrpAsn: 0.564 ± 0.155
0.846TrpPro: 0.846 ± 0.211
0.846TrpGln: 0.846 ± 0.216
1.241TrpArg: 1.241 ± 0.252
2.031TrpSer: 2.031 ± 0.399
1.693TrpThr: 1.693 ± 0.366
1.693TrpVal: 1.693 ± 0.326
0.733TrpTrp: 0.733 ± 0.218
0.79TrpTyr: 0.79 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.031TyrAla: 2.031 ± 0.285
0.395TyrCys: 0.395 ± 0.209
1.805TyrAsp: 1.805 ± 0.312
1.749TyrGlu: 1.749 ± 0.306
0.959TyrPhe: 0.959 ± 0.278
2.37TyrGly: 2.37 ± 0.478
0.451TyrHis: 0.451 ± 0.151
0.621TyrIle: 0.621 ± 0.173
0.339TyrLys: 0.339 ± 0.153
1.58TyrLeu: 1.58 ± 0.271
0.395TyrMet: 0.395 ± 0.131
0.677TyrAsn: 0.677 ± 0.199
1.016TyrPro: 1.016 ± 0.267
0.846TyrGln: 0.846 ± 0.248
1.975TyrArg: 1.975 ± 0.413
0.903TyrSer: 0.903 ± 0.208
1.749TyrThr: 1.749 ± 0.307
1.975TyrVal: 1.975 ± 0.275
0.282TyrTrp: 0.282 ± 0.113
0.621TyrTyr: 0.621 ± 0.151
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 106 proteins (17725 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski