Beihai Nido-like virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Ronidovirineae; Euroniviridae; Ceronivirinae; Charybnivirus; Cradenivirus; Charybnivirus 1

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ32|A0A1L3KJ32_9NIDO Putative glycoprotein 2 OS=Beihai Nido-like virus 2 OX=1922351 PE=4 SV=1
MM1 pKa = 7.56ALHH4 pKa = 7.13AKK6 pKa = 9.9FLLLVLILLCDD17 pKa = 3.56YY18 pKa = 10.68ARR20 pKa = 11.84SIEE23 pKa = 4.24IVGEE27 pKa = 4.01LRR29 pKa = 11.84NLDD32 pKa = 3.78PGTVKK37 pKa = 10.65GLAARR42 pKa = 11.84GSQLTRR48 pKa = 11.84YY49 pKa = 7.63TNFVQVSRR57 pKa = 11.84PTQGTVRR64 pKa = 11.84EE65 pKa = 4.21DD66 pKa = 3.5HH67 pKa = 6.29YY68 pKa = 11.39VPHH71 pKa = 6.41YY72 pKa = 11.09VDD74 pKa = 4.45IPLDD78 pKa = 3.5TDD80 pKa = 3.65LDD82 pKa = 4.3YY83 pKa = 11.66VVTGTSSLGTDD94 pKa = 4.02FGFRR98 pKa = 11.84VKK100 pKa = 10.65VDD102 pKa = 3.63DD103 pKa = 4.31VNVEE107 pKa = 4.04VEE109 pKa = 4.05NEE111 pKa = 4.38LIYY114 pKa = 9.65TAPFYY119 pKa = 10.71RR120 pKa = 11.84DD121 pKa = 3.06SHH123 pKa = 6.7EE124 pKa = 5.47DD125 pKa = 3.37NLHH128 pKa = 4.98QMVWNWDD135 pKa = 3.58GSSLTHH141 pKa = 6.22GVHH144 pKa = 6.45IPTVCAGLSNVDD156 pKa = 3.46YY157 pKa = 11.53SFFEE161 pKa = 4.41CLRR164 pKa = 11.84TRR166 pKa = 11.84DD167 pKa = 3.48EE168 pKa = 4.3VAQLSQKK175 pKa = 10.54DD176 pKa = 3.82RR177 pKa = 11.84EE178 pKa = 4.39CRR180 pKa = 11.84YY181 pKa = 10.47APLGNEE187 pKa = 3.93YY188 pKa = 10.64LSVGNYY194 pKa = 6.53TVKK197 pKa = 10.63NPRR200 pKa = 11.84GTCLNAEE207 pKa = 4.26SQSWWGRR214 pKa = 11.84PFFTGLYY221 pKa = 6.94KK222 pKa = 10.47TGMPWIRR229 pKa = 11.84AIDD232 pKa = 3.55QWNLADD238 pKa = 5.4CGTGSCTPSGQIRR251 pKa = 11.84VLQRR255 pKa = 11.84KK256 pKa = 7.99WISGVNTAMFFKK268 pKa = 10.31STGKK272 pKa = 10.54AIANYY277 pKa = 8.12TISVSPEE284 pKa = 3.66YY285 pKa = 11.12GCDD288 pKa = 3.6FDD290 pKa = 5.31RR291 pKa = 11.84CEE293 pKa = 4.07LHH295 pKa = 7.43GPGDD299 pKa = 3.67KK300 pKa = 10.0CTLSVAGDD308 pKa = 3.61TMSITGNDD316 pKa = 3.6LEE318 pKa = 4.86TTTRR322 pKa = 11.84MNIMVIAACGDD333 pKa = 3.51YY334 pKa = 10.9SGCQPVAVYY343 pKa = 9.69RR344 pKa = 11.84WPDD347 pKa = 3.43DD348 pKa = 4.35LYY350 pKa = 10.66LTNAFPYY357 pKa = 9.88LKK359 pKa = 9.13WRR361 pKa = 11.84SVPGPVYY368 pKa = 10.77ADD370 pKa = 3.16EE371 pKa = 5.2GKK373 pKa = 8.7TKK375 pKa = 10.36FQADD379 pKa = 3.27AKK381 pKa = 9.95FFPDD385 pKa = 2.81VHH387 pKa = 7.88INEE390 pKa = 4.59EE391 pKa = 4.0FCLDD395 pKa = 3.74LGYY398 pKa = 10.76RR399 pKa = 11.84QDD401 pKa = 3.78EE402 pKa = 5.05GIAQYY407 pKa = 8.32THH409 pKa = 6.75SSNKK413 pKa = 10.55DD414 pKa = 2.59GDD416 pKa = 4.23ANKK419 pKa = 9.45PACRR423 pKa = 11.84KK424 pKa = 8.39KK425 pKa = 10.93APIISQSQRR434 pKa = 11.84EE435 pKa = 4.1FEE437 pKa = 4.13EE438 pKa = 4.62RR439 pKa = 11.84GLSIDD444 pKa = 3.06RR445 pKa = 11.84CGDD448 pKa = 2.75IRR450 pKa = 11.84LEE452 pKa = 4.07YY453 pKa = 10.88VGDD456 pKa = 3.53IDD458 pKa = 5.53MVTLKK463 pKa = 10.67YY464 pKa = 10.94GKK466 pKa = 10.54GYY468 pKa = 10.34DD469 pKa = 3.93GKK471 pKa = 10.66DD472 pKa = 3.31CEE474 pKa = 4.83DD475 pKa = 3.41LKK477 pKa = 11.19KK478 pKa = 11.04VNPHH482 pKa = 6.91PYY484 pKa = 9.66RR485 pKa = 11.84YY486 pKa = 9.24PLSAVKK492 pKa = 9.97AYY494 pKa = 11.25YY495 pKa = 9.83NGCTGGVARR504 pKa = 11.84AYY506 pKa = 10.69FDD508 pKa = 3.32IKK510 pKa = 10.2STEE513 pKa = 4.02IVIDD517 pKa = 4.1PPDD520 pKa = 3.98GVDD523 pKa = 5.49LSVCQIKK530 pKa = 9.38MEE532 pKa = 4.67GYY534 pKa = 9.38YY535 pKa = 11.05GLDD538 pKa = 2.95NGITLTIGGCTVSGPVRR555 pKa = 11.84LQLPQLDD562 pKa = 3.83AYY564 pKa = 10.74FYY566 pKa = 11.15GDD568 pKa = 3.82SKK570 pKa = 10.27TLTTVLGASEE580 pKa = 4.17SRR582 pKa = 11.84ITITMLSYY590 pKa = 11.54YY591 pKa = 10.36DD592 pKa = 3.53VLLIAEE598 pKa = 4.72VFSSTITEE606 pKa = 4.4VPVTALNADD615 pKa = 3.83DD616 pKa = 5.97AYY618 pKa = 9.41TPDD621 pKa = 4.45VIPPPSNDD629 pKa = 2.79GGGFFDD635 pKa = 5.14FLNNMFGNTFSIILIISAVILVGFILVSCCASARR669 pKa = 11.84GGVKK673 pKa = 10.04KK674 pKa = 10.69EE675 pKa = 4.07SAA677 pKa = 3.51

Molecular weight: 74.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIW8|A0A1L3KIW8_9NIDO Putative glycoprotein 1 OS=Beihai Nido-like virus 2 OX=1922351 PE=4 SV=1
MM1 pKa = 7.81PRR3 pKa = 11.84PQPRR7 pKa = 11.84PPSHH11 pKa = 6.59RR12 pKa = 11.84GSRR15 pKa = 11.84EE16 pKa = 3.37VAQAANQTCSPSPTDD31 pKa = 3.19HH32 pKa = 6.99PQPQRR37 pKa = 11.84GIAGVTTEE45 pKa = 4.69AGTTATVAARR55 pKa = 11.84TLITRR60 pKa = 11.84EE61 pKa = 3.92GYY63 pKa = 10.27YY64 pKa = 10.48LLKK67 pKa = 10.97AGDD70 pKa = 4.15LVISRR75 pKa = 11.84QKK77 pKa = 10.98GPPEE81 pKa = 3.87VLLSVPATSRR91 pKa = 11.84VLSGHH96 pKa = 6.25TIGSPP101 pKa = 3.06

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

8003

101

5487

889.2

100.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.373 ± 0.345

2.512 ± 0.273

5.261 ± 0.394

4.223 ± 0.274

4.286 ± 0.348

5.71 ± 0.727

3.249 ± 0.314

5.948 ± 0.226

5.061 ± 0.274

9.072 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.351

4.686 ± 0.233

4.798 ± 0.485

3.811 ± 0.429

4.448 ± 0.69

7.06 ± 0.523

6.722 ± 0.391

7.747 ± 0.586

1.025 ± 0.234

5.91 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski