Neolamprologus brichardi (Fairy cichlid) (Lamprologus brichardi)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; <

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31886 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q4HUB6|A0A3Q4HUB6_NEOBR Ras and Rab interactor 2-like OS=Neolamprologus brichardi OX=32507 PE=3 SV=1
MM1 pKa = 7.25VLLNNLHH8 pKa = 6.89PPTEE12 pKa = 4.57GVRR15 pKa = 11.84HH16 pKa = 5.28EE17 pKa = 4.28QAEE20 pKa = 4.55TTAVMDD26 pKa = 4.39GQRR29 pKa = 11.84LGCGTLYY36 pKa = 10.64VAEE39 pKa = 4.69TRR41 pKa = 11.84LSWFDD46 pKa = 3.33SSGLGFSLEE55 pKa = 4.09YY56 pKa = 10.32PSIGLHH62 pKa = 6.16AISRR66 pKa = 11.84DD67 pKa = 3.08VSTYY71 pKa = 8.83PQEE74 pKa = 4.1HH75 pKa = 7.01LYY77 pKa = 11.61VMVNADD83 pKa = 4.5DD84 pKa = 5.95DD85 pKa = 5.32DD86 pKa = 5.99DD87 pKa = 5.3GSSDD91 pKa = 4.88GGDD94 pKa = 3.45DD95 pKa = 5.71DD96 pKa = 5.69EE97 pKa = 7.17DD98 pKa = 3.62EE99 pKa = 4.78GPITEE104 pKa = 4.09IRR106 pKa = 11.84FVPSDD111 pKa = 3.39KK112 pKa = 11.03AALEE116 pKa = 4.44SMFSAMCEE124 pKa = 4.24CQALHH129 pKa = 7.42PDD131 pKa = 4.21PEE133 pKa = 6.72DD134 pKa = 4.81DD135 pKa = 5.87DD136 pKa = 4.92SDD138 pKa = 3.91NDD140 pKa = 4.08FEE142 pKa = 4.91GEE144 pKa = 4.04EE145 pKa = 4.32YY146 pKa = 10.54DD147 pKa = 4.57VEE149 pKa = 4.43EE150 pKa = 5.21HH151 pKa = 5.7GHH153 pKa = 6.51ADD155 pKa = 2.67IPTFYY160 pKa = 10.26TCDD163 pKa = 3.46EE164 pKa = 4.43GLSALTQEE172 pKa = 4.67GQATLEE178 pKa = 4.09RR179 pKa = 11.84LEE181 pKa = 4.74GMLAQSVAQQYY192 pKa = 10.68HH193 pKa = 4.53MAGVRR198 pKa = 11.84TEE200 pKa = 3.81EE201 pKa = 4.05TKK203 pKa = 11.15AEE205 pKa = 4.17FEE207 pKa = 5.12DD208 pKa = 4.44GMEE211 pKa = 3.84VDD213 pKa = 4.04AAAMEE218 pKa = 4.63AGQFEE223 pKa = 4.69DD224 pKa = 4.94ADD226 pKa = 3.94VEE228 pKa = 4.44HH229 pKa = 7.03WW230 pKa = 3.45

Molecular weight: 25.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q4HWT0|A0A3Q4HWT0_NEOBR Mitochondrial import receptor subunit TOM20 homolog OS=Neolamprologus brichardi OX=32507 PE=3 SV=1
RR1 pKa = 6.85GAKK4 pKa = 9.76GRR6 pKa = 11.84GRR8 pKa = 11.84GGRR11 pKa = 11.84MSRR14 pKa = 11.84GGMRR18 pKa = 11.84GGRR21 pKa = 11.84GMMKK25 pKa = 10.3GFGPPGPGRR34 pKa = 11.84GRR36 pKa = 11.84VKK38 pKa = 10.62DD39 pKa = 3.39GAMNGFGPMRR49 pKa = 11.84QPLLSISGWTHH60 pKa = 3.98GHH62 pKa = 6.27GPSSSPSPTSSYY74 pKa = 10.75APQRR78 pKa = 11.84HH79 pKa = 5.74GPAPPPPPGHH89 pKa = 6.49PGFRR93 pKa = 11.84GRR95 pKa = 11.84PPHH98 pKa = 5.54PRR100 pKa = 11.84GRR102 pKa = 11.84GMPPPGPPRR111 pKa = 11.84PFRR114 pKa = 11.84PYY116 pKa = 10.33HH117 pKa = 6.06NGPVSPPPHH126 pKa = 6.87PPPGRR131 pKa = 11.84GQRR134 pKa = 11.84WPGPPGGRR142 pKa = 11.84RR143 pKa = 11.84FF144 pKa = 3.46

Molecular weight: 15.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23550

8336

31886

14967379

17

6198

469.4

52.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.357 ± 0.011

2.455 ± 0.011

5.047 ± 0.01

6.566 ± 0.017

3.96 ± 0.01

6.173 ± 0.016

2.682 ± 0.007

4.688 ± 0.011

5.724 ± 0.016

9.705 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.006

3.961 ± 0.009

5.366 ± 0.016

4.543 ± 0.013

5.451 ± 0.011

8.495 ± 0.02

5.653 ± 0.009

6.444 ± 0.012

1.215 ± 0.005

2.937 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski