Maize rough dwarf virus (MRDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Fijivirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077X598|A0A077X598_MRDV Uncharacterized protein (Fragment) OS=Maize rough dwarf virus OX=10989 GN=P5-1 PE=4 SV=1
SS1 pKa = 7.07YY2 pKa = 11.01EE3 pKa = 4.43DD4 pKa = 3.91FLTNVKK10 pKa = 10.15LVEE13 pKa = 3.88FAKK16 pKa = 10.22RR17 pKa = 11.84YY18 pKa = 9.88SSEE21 pKa = 3.73TDD23 pKa = 3.23DD24 pKa = 3.57LHH26 pKa = 7.22RR27 pKa = 11.84ATIEE31 pKa = 3.76LARR34 pKa = 11.84MSEE37 pKa = 4.2EE38 pKa = 3.82NGRR41 pKa = 11.84LLVTLKK47 pKa = 8.98QTQEE51 pKa = 3.9RR52 pKa = 11.84IANFEE57 pKa = 4.24AEE59 pKa = 4.56VMDD62 pKa = 5.04LEE64 pKa = 4.3KK65 pKa = 10.97QRR67 pKa = 11.84DD68 pKa = 4.06CFDD71 pKa = 3.84LQLTEE76 pKa = 5.97LNDD79 pKa = 4.15AYY81 pKa = 11.13CSLIKK86 pKa = 10.64EE87 pKa = 3.95KK88 pKa = 11.32DD89 pKa = 3.43EE90 pKa = 4.12IKK92 pKa = 10.15TEE94 pKa = 3.85NEE96 pKa = 3.7MLKK99 pKa = 10.29EE100 pKa = 3.84QLKK103 pKa = 9.18EE104 pKa = 3.87ANNVVVEE111 pKa = 4.51RR112 pKa = 11.84TARR115 pKa = 11.84LNEE118 pKa = 4.86AIDD121 pKa = 3.41EE122 pKa = 4.34LEE124 pKa = 4.09NVKK127 pKa = 8.76EE128 pKa = 4.15TGNAVTEE135 pKa = 4.17EE136 pKa = 4.1SDD138 pKa = 3.87EE139 pKa = 4.33VEE141 pKa = 4.25HH142 pKa = 6.73EE143 pKa = 4.16SDD145 pKa = 3.31GFRR148 pKa = 11.84LFGEE152 pKa = 5.13CIDD155 pKa = 3.72KK156 pKa = 10.78VRR158 pKa = 11.84ALKK161 pKa = 10.24EE162 pKa = 4.25KK163 pKa = 10.63YY164 pKa = 7.54PHH166 pKa = 7.04LYY168 pKa = 10.56SGVDD172 pKa = 3.31ANFGDD177 pKa = 3.81ITSLLKK183 pKa = 10.29MIMIKK188 pKa = 9.6IDD190 pKa = 4.22SIMSLCDD197 pKa = 3.25ADD199 pKa = 3.92VTVSEE204 pKa = 4.62NQWNAYY210 pKa = 7.43GLCSSVSTSTDD221 pKa = 3.29DD222 pKa = 3.93NSFPMLSVRR231 pKa = 11.84QVGLMNVQIANFTNVIYY248 pKa = 10.58DD249 pKa = 3.4AFFYY253 pKa = 11.42NMFIKK258 pKa = 10.04FLWQLSSEE266 pKa = 4.32

Molecular weight: 30.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077X4P5|A0A077X4P5_MRDV Isoform of A0A077X598 Protein P5-1 (Fragment) OS=Maize rough dwarf virus OX=10989 GN=P5-1 PE=4 SV=1
AA1 pKa = 7.86LLRR4 pKa = 11.84SSEE7 pKa = 4.1AFPVNAYY14 pKa = 9.3KK15 pKa = 10.91VSVGWNGNSFAPEE28 pKa = 3.63ITDD31 pKa = 3.19PTMIGDD37 pKa = 3.58VSLIKK42 pKa = 10.35NVSYY46 pKa = 11.24VGVHH50 pKa = 6.25SSDD53 pKa = 3.24NYY55 pKa = 10.58KK56 pKa = 10.28DD57 pKa = 3.51RR58 pKa = 11.84KK59 pKa = 8.82MFSRR63 pKa = 11.84LKK65 pKa = 9.84TFLEE69 pKa = 4.18IAANCNLVLKK79 pKa = 10.32GCRR82 pKa = 11.84TKK84 pKa = 10.61PLSIEE89 pKa = 3.98YY90 pKa = 10.6DD91 pKa = 3.12NTRR94 pKa = 11.84ISKK97 pKa = 10.25KK98 pKa = 10.38LAGKK102 pKa = 9.95KK103 pKa = 9.41ILYY106 pKa = 9.45NDD108 pKa = 3.6RR109 pKa = 11.84AHH111 pKa = 7.35IIFNGIYY118 pKa = 9.91HH119 pKa = 7.29AVTPFMNKK127 pKa = 9.16VNDD130 pKa = 3.62MLMDD134 pKa = 4.87GNTEE138 pKa = 3.56IDD140 pKa = 3.09FDD142 pKa = 4.48KK143 pKa = 10.48IYY145 pKa = 11.05KK146 pKa = 10.01FGRR149 pKa = 11.84DD150 pKa = 2.97AEE152 pKa = 4.35KK153 pKa = 10.64RR154 pKa = 11.84INTYY158 pKa = 11.66DD159 pKa = 3.75MICRR163 pKa = 11.84GYY165 pKa = 11.34GNMDD169 pKa = 3.2QFSSYY174 pKa = 10.64LVNLSTQRR182 pKa = 11.84ICDD185 pKa = 3.32NRR187 pKa = 11.84MTANHH192 pKa = 6.62YY193 pKa = 7.27KK194 pKa = 9.72TLMAANYY201 pKa = 6.8PNRR204 pKa = 11.84VSMHH208 pKa = 5.86VFDD211 pKa = 5.39TMIDD215 pKa = 3.58DD216 pKa = 5.03LLFVYY221 pKa = 10.58CPTT224 pKa = 3.95

Molecular weight: 25.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

4054

224

778

368.5

42.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.169 ± 0.368

1.727 ± 0.309

6.438 ± 0.302

5.945 ± 0.673

5.895 ± 0.406

3.823 ± 0.336

2.96 ± 0.323

6.931 ± 0.522

6.438 ± 0.294

10.927 ± 0.455

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.316

6.29 ± 0.266

3.256 ± 0.304

2.664 ± 0.209

4.243 ± 0.254

8.781 ± 0.776

5.673 ± 0.509

6.438 ± 0.444

0.641 ± 0.165

4.243 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski