Bacillus phage Deep-Purple

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Deurplevirus; Bacillus virus DeepPurple

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z1LZM9|A0A1Z1LZM9_9CAUD Major capsid protein OS=Bacillus phage Deep-Purple OX=1873341 GN=DeepPurple_gp024 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.43NLEE5 pKa = 4.17TAIKK9 pKa = 10.19DD10 pKa = 3.44IISQKK15 pKa = 10.42LEE17 pKa = 4.07SGVVEE22 pKa = 4.22TLVEE26 pKa = 4.04EE27 pKa = 4.07QLQVSINHH35 pKa = 6.3ALKK38 pKa = 10.68DD39 pKa = 3.47IFGSYY44 pKa = 11.24GDD46 pKa = 3.65VTKK49 pKa = 10.42MLEE52 pKa = 4.31GKK54 pKa = 9.67LKK56 pKa = 10.67EE57 pKa = 4.53SIAPFLEE64 pKa = 4.54SYY66 pKa = 10.43DD67 pKa = 3.54YY68 pKa = 11.53SRR70 pKa = 11.84FMVKK74 pKa = 10.01VEE76 pKa = 4.16SVLDD80 pKa = 3.54NVLRR84 pKa = 11.84EE85 pKa = 3.94TALPDD90 pKa = 2.98KK91 pKa = 10.6TLIEE95 pKa = 4.24NVGKK99 pKa = 10.32LLEE102 pKa = 4.58NKK104 pKa = 9.65SVPKK108 pKa = 9.64LVKK111 pKa = 10.06MEE113 pKa = 4.74DD114 pKa = 5.26LFTAWCKK121 pKa = 9.72MLAEE125 pKa = 4.24NVEE128 pKa = 4.31TLGLDD133 pKa = 3.01IDD135 pKa = 4.54YY136 pKa = 11.06EE137 pKa = 4.5DD138 pKa = 5.45GPTFDD143 pKa = 5.24AVPVLCEE150 pKa = 3.45IHH152 pKa = 6.58EE153 pKa = 4.66DD154 pKa = 3.91EE155 pKa = 4.59RR156 pKa = 11.84SSWSSIEE163 pKa = 3.91SLTIEE168 pKa = 5.06FSCEE172 pKa = 3.02HH173 pKa = 7.1DD174 pKa = 3.36PRR176 pKa = 11.84HH177 pKa = 5.54NVSFSASRR185 pKa = 11.84WKK187 pKa = 10.83SSSNKK192 pKa = 9.58NWDD195 pKa = 3.85LEE197 pKa = 4.75VKK199 pKa = 10.62LSTDD203 pKa = 2.88IKK205 pKa = 11.01DD206 pKa = 3.33IANMNEE212 pKa = 3.68FEE214 pKa = 4.31IFLMRR219 pKa = 11.84LQRR222 pKa = 11.84NFADD226 pKa = 3.7VSIEE230 pKa = 3.74RR231 pKa = 11.84DD232 pKa = 3.19DD233 pKa = 4.6YY234 pKa = 11.37YY235 pKa = 11.44VEE237 pKa = 5.06EE238 pKa = 4.77YY239 pKa = 8.83VTPTAEE245 pKa = 4.17PEE247 pKa = 3.85ATYY250 pKa = 11.19SS251 pKa = 3.32

Molecular weight: 28.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z1LZR2|A0A1Z1LZR2_9CAUD Uncharacterized protein OS=Bacillus phage Deep-Purple OX=1873341 GN=DeepPurple_gp031 PE=4 SV=1
MM1 pKa = 7.73ALRR4 pKa = 11.84YY5 pKa = 9.99VGGDD9 pKa = 3.18IVKK12 pKa = 10.24SPYY15 pKa = 10.6DD16 pKa = 3.58FYY18 pKa = 11.05ISPEE22 pKa = 3.98EE23 pKa = 3.92YY24 pKa = 10.36KK25 pKa = 10.31IAEE28 pKa = 4.24SNGICKK34 pKa = 8.68KK35 pKa = 7.91TLEE38 pKa = 3.82YY39 pKa = 10.39RR40 pKa = 11.84IRR42 pKa = 11.84SGGWDD47 pKa = 3.45KK48 pKa = 11.08QRR50 pKa = 11.84AITDD54 pKa = 4.82PIQYY58 pKa = 10.06KK59 pKa = 9.31GEE61 pKa = 3.71EE62 pKa = 4.26WISVKK67 pKa = 10.44EE68 pKa = 3.78IALKK72 pKa = 10.88NGISRR77 pKa = 11.84KK78 pKa = 7.82TFTQRR83 pKa = 11.84RR84 pKa = 11.84KK85 pKa = 10.26RR86 pKa = 11.84GWDD89 pKa = 3.14LVEE92 pKa = 4.82SYY94 pKa = 9.19TRR96 pKa = 11.84PPLTRR101 pKa = 11.84KK102 pKa = 9.09EE103 pKa = 3.95ALQRR107 pKa = 11.84ATASSKK113 pKa = 9.76RR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84VFTDD120 pKa = 3.25EE121 pKa = 3.68EE122 pKa = 4.39LKK124 pKa = 10.88RR125 pKa = 11.84ADD127 pKa = 3.46NIGIKK132 pKa = 10.24RR133 pKa = 11.84STLYY137 pKa = 10.53HH138 pKa = 6.72RR139 pKa = 11.84NNALKK144 pKa = 9.73WPKK147 pKa = 10.01SRR149 pKa = 11.84CLSRR153 pKa = 11.84EE154 pKa = 3.5TLTRR158 pKa = 11.84SEE160 pKa = 4.53CGRR163 pKa = 11.84AGAISYY169 pKa = 7.57MYY171 pKa = 9.86QIRR174 pKa = 11.84NLPISGLAKK183 pKa = 10.16AVQTII188 pKa = 3.59

Molecular weight: 21.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

10557

41

2156

263.9

29.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.436 ± 0.383

0.578 ± 0.118

5.911 ± 0.303

7.19 ± 0.544

3.969 ± 0.247

5.854 ± 0.486

1.601 ± 0.151

7.18 ± 0.37

8.203 ± 0.327

8.175 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.334 ± 0.413

5.826 ± 0.384

2.861 ± 0.174

3.931 ± 0.343

4.329 ± 0.252

5.769 ± 0.247

6.716 ± 0.515

6.413 ± 0.242

1.099 ± 0.155

3.628 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski