Amino acid dipepetide frequency for Bacillus phage Deep-Purple

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.474AlaCys: 0.474 ± 0.27
4.074AlaAsp: 4.074 ± 0.371
6.252AlaGlu: 6.252 ± 0.761
3.41AlaPhe: 3.41 ± 0.508
4.452AlaGly: 4.452 ± 0.541
0.474AlaHis: 0.474 ± 0.195
6.537AlaIle: 6.537 ± 0.79
7.389AlaLys: 7.389 ± 1.135
7.579AlaLeu: 7.579 ± 1.172
2.747AlaMet: 2.747 ± 0.928
4.074AlaAsn: 4.074 ± 0.694
2.842AlaPro: 2.842 ± 0.464
2.842AlaGln: 2.842 ± 0.546
3.221AlaArg: 3.221 ± 0.579
4.737AlaSer: 4.737 ± 0.831
5.873AlaThr: 5.873 ± 0.685
3.789AlaVal: 3.789 ± 0.599
1.042AlaTrp: 1.042 ± 0.332
2.558AlaTyr: 2.558 ± 0.496
0.0AlaXaa: 0.0 ± 0.0
Cys
0.284CysAla: 0.284 ± 0.176
0.0CysCys: 0.0 ± 0.0
0.284CysAsp: 0.284 ± 0.211
0.379CysGlu: 0.379 ± 0.24
0.379CysPhe: 0.379 ± 0.195
0.284CysGly: 0.284 ± 0.191
0.284CysHis: 0.284 ± 0.187
0.189CysIle: 0.189 ± 0.152
0.663CysLys: 0.663 ± 0.301
0.663CysLeu: 0.663 ± 0.364
0.189CysMet: 0.189 ± 0.172
0.284CysAsn: 0.284 ± 0.219
0.284CysPro: 0.284 ± 0.167
0.189CysGln: 0.189 ± 0.154
0.379CysArg: 0.379 ± 0.18
0.284CysSer: 0.284 ± 0.181
0.284CysThr: 0.284 ± 0.18
0.189CysVal: 0.189 ± 0.114
0.0CysTrp: 0.0 ± 0.0
0.284CysTyr: 0.284 ± 0.248
0.0CysXaa: 0.0 ± 0.0
Asp
4.074AspAla: 4.074 ± 0.525
0.189AspCys: 0.189 ± 0.113
2.747AspAsp: 2.747 ± 0.611
3.884AspGlu: 3.884 ± 0.871
3.316AspPhe: 3.316 ± 0.591
4.452AspGly: 4.452 ± 0.54
0.568AspHis: 0.568 ± 0.247
5.21AspIle: 5.21 ± 0.98
5.4AspLys: 5.4 ± 0.602
3.789AspLeu: 3.789 ± 0.738
2.179AspMet: 2.179 ± 0.305
3.41AspAsn: 3.41 ± 0.52
1.516AspPro: 1.516 ± 0.615
1.137AspGln: 1.137 ± 0.217
2.747AspArg: 2.747 ± 0.453
2.842AspSer: 2.842 ± 0.387
3.505AspThr: 3.505 ± 0.704
5.021AspVal: 5.021 ± 0.877
0.758AspTrp: 0.758 ± 0.275
2.368AspTyr: 2.368 ± 0.518
0.0AspXaa: 0.0 ± 0.0
Glu
6.252GluAla: 6.252 ± 0.689
0.189GluCys: 0.189 ± 0.135
4.358GluAsp: 4.358 ± 0.643
5.021GluGlu: 5.021 ± 1.028
2.653GluPhe: 2.653 ± 0.501
4.168GluGly: 4.168 ± 0.63
1.232GluHis: 1.232 ± 0.321
5.021GluIle: 5.021 ± 0.741
4.547GluLys: 4.547 ± 0.79
6.821GluLeu: 6.821 ± 0.88
2.463GluMet: 2.463 ± 0.716
3.031GluAsn: 3.031 ± 0.617
1.705GluPro: 1.705 ± 0.381
4.547GluGln: 4.547 ± 0.887
3.41GluArg: 3.41 ± 0.903
3.41GluSer: 3.41 ± 0.593
3.41GluThr: 3.41 ± 0.49
6.063GluVal: 6.063 ± 0.828
1.326GluTrp: 1.326 ± 0.406
2.274GluTyr: 2.274 ± 0.501
0.0GluXaa: 0.0 ± 0.0
Phe
2.084PheAla: 2.084 ± 0.438
0.663PheCys: 0.663 ± 0.276
2.937PheAsp: 2.937 ± 0.714
2.463PheGlu: 2.463 ± 0.532
1.421PhePhe: 1.421 ± 0.297
1.895PheGly: 1.895 ± 0.332
0.663PheHis: 0.663 ± 0.258
2.653PheIle: 2.653 ± 0.47
3.979PheLys: 3.979 ± 0.604
3.126PheLeu: 3.126 ± 0.549
1.516PheMet: 1.516 ± 0.437
2.747PheAsn: 2.747 ± 0.545
0.947PhePro: 0.947 ± 0.313
0.947PheGln: 0.947 ± 0.3
1.232PheArg: 1.232 ± 0.401
2.558PheSer: 2.558 ± 0.459
2.558PheThr: 2.558 ± 0.402
2.842PheVal: 2.842 ± 0.621
0.379PheTrp: 0.379 ± 0.246
2.084PheTyr: 2.084 ± 0.462
0.0PheXaa: 0.0 ± 0.0
Gly
3.6GlyAla: 3.6 ± 0.636
0.095GlyCys: 0.095 ± 0.126
3.884GlyAsp: 3.884 ± 0.529
3.6GlyGlu: 3.6 ± 0.616
2.084GlyPhe: 2.084 ± 0.48
3.126GlyGly: 3.126 ± 0.625
0.853GlyHis: 0.853 ± 0.277
5.495GlyIle: 5.495 ± 0.978
5.4GlyLys: 5.4 ± 0.568
5.305GlyLeu: 5.305 ± 0.924
1.705GlyMet: 1.705 ± 0.447
3.316GlyAsn: 3.316 ± 0.616
0.853GlyPro: 0.853 ± 0.254
1.989GlyGln: 1.989 ± 0.532
2.368GlyArg: 2.368 ± 0.525
3.126GlySer: 3.126 ± 0.597
4.642GlyThr: 4.642 ± 1.121
3.6GlyVal: 3.6 ± 0.612
0.947GlyTrp: 0.947 ± 0.263
2.558GlyTyr: 2.558 ± 0.418
0.0GlyXaa: 0.0 ± 0.0
His
1.326HisAla: 1.326 ± 0.428
0.095HisCys: 0.095 ± 0.12
0.947HisAsp: 0.947 ± 0.266
1.232HisGlu: 1.232 ± 0.386
0.568HisPhe: 0.568 ± 0.278
0.853HisGly: 0.853 ± 0.212
0.758HisHis: 0.758 ± 0.301
0.758HisIle: 0.758 ± 0.296
1.232HisLys: 1.232 ± 0.433
1.705HisLeu: 1.705 ± 0.395
0.663HisMet: 0.663 ± 0.246
0.853HisAsn: 0.853 ± 0.25
0.379HisPro: 0.379 ± 0.176
0.095HisGln: 0.095 ± 0.12
0.379HisArg: 0.379 ± 0.181
0.474HisSer: 0.474 ± 0.223
1.61HisThr: 1.61 ± 0.364
1.042HisVal: 1.042 ± 0.3
0.095HisTrp: 0.095 ± 0.118
0.947HisTyr: 0.947 ± 0.399
0.0HisXaa: 0.0 ± 0.0
Ile
5.779IleAla: 5.779 ± 0.781
0.663IleCys: 0.663 ± 0.316
4.547IleAsp: 4.547 ± 0.539
6.252IleGlu: 6.252 ± 0.834
2.084IlePhe: 2.084 ± 0.483
4.168IleGly: 4.168 ± 1.121
1.042IleHis: 1.042 ± 0.257
4.926IleIle: 4.926 ± 0.799
7.294IleLys: 7.294 ± 0.766
4.074IleLeu: 4.074 ± 0.888
1.705IleMet: 1.705 ± 0.339
5.21IleAsn: 5.21 ± 0.663
1.705IlePro: 1.705 ± 0.375
2.558IleGln: 2.558 ± 0.564
3.6IleArg: 3.6 ± 0.5
4.831IleSer: 4.831 ± 0.819
4.547IleThr: 4.547 ± 0.656
4.642IleVal: 4.642 ± 0.764
0.568IleTrp: 0.568 ± 0.28
1.61IleTyr: 1.61 ± 0.356
0.0IleXaa: 0.0 ± 0.0
Lys
7.2LysAla: 7.2 ± 1.092
0.284LysCys: 0.284 ± 0.149
4.831LysAsp: 4.831 ± 0.577
6.252LysGlu: 6.252 ± 1.051
3.126LysPhe: 3.126 ± 0.584
4.074LysGly: 4.074 ± 0.565
1.516LysHis: 1.516 ± 0.301
3.979LysIle: 3.979 ± 0.922
5.589LysLys: 5.589 ± 0.892
7.768LysLeu: 7.768 ± 1.175
3.41LysMet: 3.41 ± 0.693
3.505LysAsn: 3.505 ± 0.445
2.084LysPro: 2.084 ± 0.321
4.168LysGln: 4.168 ± 0.697
3.221LysArg: 3.221 ± 0.896
5.116LysSer: 5.116 ± 0.722
6.347LysThr: 6.347 ± 0.992
5.4LysVal: 5.4 ± 0.81
1.137LysTrp: 1.137 ± 0.32
3.031LysTyr: 3.031 ± 0.477
0.0LysXaa: 0.0 ± 0.0
Leu
4.926LeuAla: 4.926 ± 0.724
0.947LeuCys: 0.947 ± 0.403
5.305LeuAsp: 5.305 ± 0.635
5.968LeuGlu: 5.968 ± 0.815
2.179LeuPhe: 2.179 ± 0.379
5.4LeuGly: 5.4 ± 0.82
1.516LeuHis: 1.516 ± 0.331
6.158LeuIle: 6.158 ± 0.781
6.158LeuLys: 6.158 ± 0.734
6.631LeuLeu: 6.631 ± 0.915
2.274LeuMet: 2.274 ± 0.464
5.305LeuAsn: 5.305 ± 0.763
3.695LeuPro: 3.695 ± 1.135
3.6LeuGln: 3.6 ± 0.46
2.463LeuArg: 2.463 ± 0.46
4.358LeuSer: 4.358 ± 1.128
5.589LeuThr: 5.589 ± 0.49
5.116LeuVal: 5.116 ± 0.645
1.137LeuTrp: 1.137 ± 0.371
2.937LeuTyr: 2.937 ± 0.547
0.0LeuXaa: 0.0 ± 0.0
Met
3.221MetAla: 3.221 ± 0.982
0.189MetCys: 0.189 ± 0.166
1.516MetAsp: 1.516 ± 0.476
1.705MetGlu: 1.705 ± 0.403
1.516MetPhe: 1.516 ± 0.44
1.989MetGly: 1.989 ± 0.709
0.758MetHis: 0.758 ± 0.254
1.895MetIle: 1.895 ± 0.572
2.937MetLys: 2.937 ± 0.346
2.463MetLeu: 2.463 ± 0.605
1.042MetMet: 1.042 ± 0.564
1.705MetAsn: 1.705 ± 0.551
1.042MetPro: 1.042 ± 0.282
1.61MetGln: 1.61 ± 0.373
2.179MetArg: 2.179 ± 0.424
2.842MetSer: 2.842 ± 0.542
1.8MetThr: 1.8 ± 0.444
1.137MetVal: 1.137 ± 0.311
0.189MetTrp: 0.189 ± 0.147
1.61MetTyr: 1.61 ± 0.302
0.0MetXaa: 0.0 ± 0.0
Asn
4.737AsnAla: 4.737 ± 0.593
0.284AsnCys: 0.284 ± 0.128
3.221AsnAsp: 3.221 ± 0.597
4.074AsnGlu: 4.074 ± 0.49
2.463AsnPhe: 2.463 ± 0.527
4.547AsnGly: 4.547 ± 0.947
0.947AsnHis: 0.947 ± 0.338
3.789AsnIle: 3.789 ± 1.068
3.695AsnLys: 3.695 ± 0.626
4.547AsnLeu: 4.547 ± 0.864
2.179AsnMet: 2.179 ± 0.591
4.074AsnAsn: 4.074 ± 0.86
1.705AsnPro: 1.705 ± 0.355
1.895AsnGln: 1.895 ± 0.634
2.463AsnArg: 2.463 ± 0.574
3.221AsnSer: 3.221 ± 0.639
4.737AsnThr: 4.737 ± 0.801
3.789AsnVal: 3.789 ± 0.772
0.663AsnTrp: 0.663 ± 0.233
1.232AsnTyr: 1.232 ± 0.354
0.0AsnXaa: 0.0 ± 0.0
Pro
3.316ProAla: 3.316 ± 0.522
0.095ProCys: 0.095 ± 0.093
2.179ProAsp: 2.179 ± 0.419
2.084ProGlu: 2.084 ± 0.383
1.326ProPhe: 1.326 ± 0.325
0.189ProGly: 0.189 ± 0.119
0.474ProHis: 0.474 ± 0.183
1.705ProIle: 1.705 ± 0.522
2.179ProLys: 2.179 ± 0.436
1.516ProLeu: 1.516 ± 0.357
0.853ProMet: 0.853 ± 0.28
1.232ProAsn: 1.232 ± 0.306
0.663ProPro: 0.663 ± 0.266
1.137ProGln: 1.137 ± 0.409
1.042ProArg: 1.042 ± 0.333
2.368ProSer: 2.368 ± 0.556
2.558ProThr: 2.558 ± 0.506
1.989ProVal: 1.989 ± 0.436
0.284ProTrp: 0.284 ± 0.234
1.421ProTyr: 1.421 ± 0.42
0.0ProXaa: 0.0 ± 0.0
Gln
3.789GlnAla: 3.789 ± 0.485
0.095GlnCys: 0.095 ± 0.078
1.989GlnAsp: 1.989 ± 0.339
2.842GlnGlu: 2.842 ± 0.719
1.8GlnPhe: 1.8 ± 0.662
2.842GlnGly: 2.842 ± 0.566
0.379GlnHis: 0.379 ± 0.21
3.884GlnIle: 3.884 ± 0.693
2.084GlnLys: 2.084 ± 0.485
3.41GlnLeu: 3.41 ± 0.638
1.421GlnMet: 1.421 ± 0.386
2.274GlnAsn: 2.274 ± 0.55
0.379GlnPro: 0.379 ± 0.2
1.705GlnGln: 1.705 ± 0.646
2.463GlnArg: 2.463 ± 0.42
1.8GlnSer: 1.8 ± 0.364
2.084GlnThr: 2.084 ± 0.389
2.274GlnVal: 2.274 ± 0.458
0.284GlnTrp: 0.284 ± 0.124
1.516GlnTyr: 1.516 ± 0.31
0.0GlnXaa: 0.0 ± 0.0
Arg
2.842ArgAla: 2.842 ± 0.745
0.189ArgCys: 0.189 ± 0.144
2.179ArgAsp: 2.179 ± 0.434
3.695ArgGlu: 3.695 ± 0.602
2.179ArgPhe: 2.179 ± 0.384
2.842ArgGly: 2.842 ± 0.568
0.568ArgHis: 0.568 ± 0.306
2.747ArgIle: 2.747 ± 0.507
3.789ArgLys: 3.789 ± 1.039
4.168ArgLeu: 4.168 ± 0.708
0.853ArgMet: 0.853 ± 0.33
2.747ArgAsn: 2.747 ± 0.598
1.042ArgPro: 1.042 ± 0.304
1.042ArgGln: 1.042 ± 0.228
2.747ArgArg: 2.747 ± 0.514
1.895ArgSer: 1.895 ± 0.513
2.274ArgThr: 2.274 ± 0.372
3.221ArgVal: 3.221 ± 0.605
0.663ArgTrp: 0.663 ± 0.191
1.61ArgTyr: 1.61 ± 0.294
0.0ArgXaa: 0.0 ± 0.0
Ser
4.642SerAla: 4.642 ± 0.647
0.379SerCys: 0.379 ± 0.248
3.221SerAsp: 3.221 ± 0.47
2.653SerGlu: 2.653 ± 0.517
2.179SerPhe: 2.179 ± 0.469
3.221SerGly: 3.221 ± 0.671
1.232SerHis: 1.232 ± 0.356
4.358SerIle: 4.358 ± 0.702
4.831SerLys: 4.831 ± 0.696
4.358SerLeu: 4.358 ± 0.769
1.989SerMet: 1.989 ± 0.377
3.41SerAsn: 3.41 ± 0.63
2.558SerPro: 2.558 ± 0.463
2.274SerGln: 2.274 ± 0.33
2.463SerArg: 2.463 ± 0.496
4.168SerSer: 4.168 ± 0.823
3.6SerThr: 3.6 ± 0.469
3.695SerVal: 3.695 ± 0.603
0.379SerTrp: 0.379 ± 0.176
2.084SerTyr: 2.084 ± 0.506
0.0SerXaa: 0.0 ± 0.0
Thr
7.105ThrAla: 7.105 ± 1.212
0.379ThrCys: 0.379 ± 0.178
3.221ThrAsp: 3.221 ± 0.729
4.642ThrGlu: 4.642 ± 0.842
3.316ThrPhe: 3.316 ± 0.592
4.168ThrGly: 4.168 ± 0.792
1.137ThrHis: 1.137 ± 0.353
5.116ThrIle: 5.116 ± 0.508
4.642ThrLys: 4.642 ± 0.682
6.347ThrLeu: 6.347 ± 0.741
1.895ThrMet: 1.895 ± 0.378
4.642ThrAsn: 4.642 ± 0.629
2.368ThrPro: 2.368 ± 0.685
2.558ThrGln: 2.558 ± 0.727
2.179ThrArg: 2.179 ± 0.397
2.937ThrSer: 2.937 ± 0.44
4.168ThrThr: 4.168 ± 0.563
3.41ThrVal: 3.41 ± 0.883
0.379ThrTrp: 0.379 ± 0.166
2.558ThrTyr: 2.558 ± 0.647
0.0ThrXaa: 0.0 ± 0.0
Val
5.4ValAla: 5.4 ± 0.805
0.284ValCys: 0.284 ± 0.125
4.358ValAsp: 4.358 ± 0.568
6.347ValGlu: 6.347 ± 0.786
1.705ValPhe: 1.705 ± 0.534
3.316ValGly: 3.316 ± 0.574
0.568ValHis: 0.568 ± 0.219
4.642ValIle: 4.642 ± 0.548
6.347ValLys: 6.347 ± 0.713
3.979ValLeu: 3.979 ± 0.599
2.179ValMet: 2.179 ± 0.66
3.126ValAsn: 3.126 ± 0.487
1.895ValPro: 1.895 ± 0.468
3.031ValGln: 3.031 ± 0.575
3.031ValArg: 3.031 ± 0.59
4.168ValSer: 4.168 ± 0.624
3.789ValThr: 3.789 ± 0.811
3.316ValVal: 3.316 ± 0.498
0.474ValTrp: 0.474 ± 0.225
2.179ValTyr: 2.179 ± 0.542
0.0ValXaa: 0.0 ± 0.0
Trp
0.758TrpAla: 0.758 ± 0.281
0.189TrpCys: 0.189 ± 0.14
1.042TrpAsp: 1.042 ± 0.337
0.663TrpGlu: 0.663 ± 0.257
0.568TrpPhe: 0.568 ± 0.208
0.568TrpGly: 0.568 ± 0.265
0.284TrpHis: 0.284 ± 0.211
1.042TrpIle: 1.042 ± 0.248
0.758TrpLys: 0.758 ± 0.257
0.663TrpLeu: 0.663 ± 0.177
0.0TrpMet: 0.0 ± 0.0
0.568TrpAsn: 0.568 ± 0.307
0.189TrpPro: 0.189 ± 0.155
0.568TrpGln: 0.568 ± 0.249
0.284TrpArg: 0.284 ± 0.134
0.758TrpSer: 0.758 ± 0.257
0.853TrpThr: 0.853 ± 0.389
1.042TrpVal: 1.042 ± 0.3
0.0TrpTrp: 0.0 ± 0.0
0.189TrpTyr: 0.189 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.031TyrAla: 3.031 ± 0.537
0.095TyrCys: 0.095 ± 0.115
2.274TyrAsp: 2.274 ± 0.604
1.895TyrGlu: 1.895 ± 0.52
1.421TyrPhe: 1.421 ± 0.519
1.989TyrGly: 1.989 ± 0.347
0.758TyrHis: 0.758 ± 0.275
1.705TyrIle: 1.705 ± 0.323
3.126TyrLys: 3.126 ± 0.793
2.842TyrLeu: 2.842 ± 0.48
1.989TyrMet: 1.989 ± 0.388
2.747TyrAsn: 2.747 ± 0.694
0.758TyrPro: 0.758 ± 0.253
1.421TyrGln: 1.421 ± 0.322
1.421TyrArg: 1.421 ± 0.356
1.8TyrSer: 1.8 ± 0.457
2.937TyrThr: 2.937 ± 0.588
2.558TyrVal: 2.558 ± 0.469
0.284TyrTrp: 0.284 ± 0.216
1.232TyrTyr: 1.232 ± 0.47
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 40 proteins (10557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski