Amino acid dipepetide frequency for Lentibacillus sp. JNUCC-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.371AlaAla: 6.371 ± 0.1
0.628AlaCys: 0.628 ± 0.028
4.207AlaAsp: 4.207 ± 0.071
5.129AlaGlu: 5.129 ± 0.079
3.686AlaPhe: 3.686 ± 0.07
5.75AlaGly: 5.75 ± 0.092
1.662AlaHis: 1.662 ± 0.045
5.734AlaIle: 5.734 ± 0.079
4.281AlaLys: 4.281 ± 0.068
7.913AlaLeu: 7.913 ± 0.097
2.298AlaMet: 2.298 ± 0.047
2.61AlaAsn: 2.61 ± 0.052
2.3AlaPro: 2.3 ± 0.048
2.559AlaGln: 2.559 ± 0.054
3.067AlaArg: 3.067 ± 0.058
4.117AlaSer: 4.117 ± 0.068
3.576AlaThr: 3.576 ± 0.061
5.864AlaVal: 5.864 ± 0.078
0.754AlaTrp: 0.754 ± 0.03
2.662AlaTyr: 2.662 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.436CysAla: 0.436 ± 0.023
0.086CysCys: 0.086 ± 0.009
0.412CysAsp: 0.412 ± 0.022
0.415CysGlu: 0.415 ± 0.022
0.271CysPhe: 0.271 ± 0.017
0.641CysGly: 0.641 ± 0.028
0.22CysHis: 0.22 ± 0.014
0.408CysIle: 0.408 ± 0.023
0.31CysLys: 0.31 ± 0.02
0.541CysLeu: 0.541 ± 0.022
0.185CysMet: 0.185 ± 0.015
0.223CysAsn: 0.223 ± 0.017
0.356CysPro: 0.356 ± 0.02
0.223CysGln: 0.223 ± 0.015
0.298CysArg: 0.298 ± 0.016
0.449CysSer: 0.449 ± 0.023
0.365CysThr: 0.365 ± 0.019
0.408CysVal: 0.408 ± 0.022
0.056CysTrp: 0.056 ± 0.008
0.2CysTyr: 0.2 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
4.01AspAla: 4.01 ± 0.065
0.305AspCys: 0.305 ± 0.019
3.267AspAsp: 3.267 ± 0.068
4.795AspGlu: 4.795 ± 0.085
2.433AspPhe: 2.433 ± 0.056
3.654AspGly: 3.654 ± 0.076
1.66AspHis: 1.66 ± 0.044
4.593AspIle: 4.593 ± 0.075
3.289AspLys: 3.289 ± 0.069
5.38AspLeu: 5.38 ± 0.079
1.894AspMet: 1.894 ± 0.038
2.015AspAsn: 2.015 ± 0.043
2.368AspPro: 2.368 ± 0.056
2.654AspGln: 2.654 ± 0.057
2.537AspArg: 2.537 ± 0.054
2.589AspSer: 2.589 ± 0.051
2.962AspThr: 2.962 ± 0.056
4.566AspVal: 4.566 ± 0.076
0.695AspTrp: 0.695 ± 0.03
2.313AspTyr: 2.313 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
6.192GluAla: 6.192 ± 0.1
0.292GluCys: 0.292 ± 0.017
4.281GluAsp: 4.281 ± 0.087
6.332GluGlu: 6.332 ± 0.12
2.209GluPhe: 2.209 ± 0.048
4.36GluGly: 4.36 ± 0.069
1.793GluHis: 1.793 ± 0.047
4.615GluIle: 4.615 ± 0.076
5.739GluLys: 5.739 ± 0.093
6.469GluLeu: 6.469 ± 0.085
2.179GluMet: 2.179 ± 0.048
3.293GluAsn: 3.293 ± 0.067
2.134GluPro: 2.134 ± 0.047
3.604GluGln: 3.604 ± 0.074
3.693GluArg: 3.693 ± 0.065
3.494GluSer: 3.494 ± 0.065
4.474GluThr: 4.474 ± 0.079
4.577GluVal: 4.577 ± 0.083
0.812GluTrp: 0.812 ± 0.032
1.979GluTyr: 1.979 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
3.046PheAla: 3.046 ± 0.062
0.323PheCys: 0.323 ± 0.018
2.475PheAsp: 2.475 ± 0.05
2.603PheGlu: 2.603 ± 0.046
2.139PhePhe: 2.139 ± 0.061
3.199PheGly: 3.199 ± 0.073
0.95PheHis: 0.95 ± 0.034
3.521PheIle: 3.521 ± 0.069
2.504PheLys: 2.504 ± 0.046
4.179PheLeu: 4.179 ± 0.085
1.287PheMet: 1.287 ± 0.031
1.956PheAsn: 1.956 ± 0.041
1.654PhePro: 1.654 ± 0.043
1.502PheGln: 1.502 ± 0.04
1.555PheArg: 1.555 ± 0.042
3.007PheSer: 3.007 ± 0.05
2.602PheThr: 2.602 ± 0.059
3.065PheVal: 3.065 ± 0.068
0.497PheTrp: 0.497 ± 0.025
1.613PheTyr: 1.613 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
5.133GlyAla: 5.133 ± 0.088
0.563GlyCys: 0.563 ± 0.023
3.689GlyAsp: 3.689 ± 0.063
4.435GlyGlu: 4.435 ± 0.082
3.354GlyPhe: 3.354 ± 0.067
4.852GlyGly: 4.852 ± 0.084
1.621GlyHis: 1.621 ± 0.041
5.563GlyIle: 5.563 ± 0.084
4.474GlyLys: 4.474 ± 0.068
6.605GlyLeu: 6.605 ± 0.099
2.306GlyMet: 2.306 ± 0.047
2.581GlyAsn: 2.581 ± 0.056
1.875GlyPro: 1.875 ± 0.042
2.255GlyGln: 2.255 ± 0.048
2.782GlyArg: 2.782 ± 0.055
3.867GlySer: 3.867 ± 0.065
4.066GlyThr: 4.066 ± 0.069
5.337GlyVal: 5.337 ± 0.08
0.813GlyTrp: 0.813 ± 0.033
2.894GlyTyr: 2.894 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.681HisAla: 1.681 ± 0.042
0.19HisCys: 0.19 ± 0.012
1.345HisAsp: 1.345 ± 0.033
1.623HisGlu: 1.623 ± 0.041
1.11HisPhe: 1.11 ± 0.03
1.554HisGly: 1.554 ± 0.043
0.807HisHis: 0.807 ± 0.03
1.909HisIle: 1.909 ± 0.045
1.209HisLys: 1.209 ± 0.036
2.315HisLeu: 2.315 ± 0.047
0.742HisMet: 0.742 ± 0.03
0.903HisAsn: 0.903 ± 0.03
1.164HisPro: 1.164 ± 0.034
0.981HisGln: 0.981 ± 0.03
0.978HisArg: 0.978 ± 0.03
1.171HisSer: 1.171 ± 0.036
1.314HisThr: 1.314 ± 0.036
1.817HisVal: 1.817 ± 0.045
0.274HisTrp: 0.274 ± 0.016
0.947HisTyr: 0.947 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.928IleAla: 5.928 ± 0.083
0.53IleCys: 0.53 ± 0.025
4.57IleAsp: 4.57 ± 0.077
5.245IleGlu: 5.245 ± 0.089
2.985IlePhe: 2.985 ± 0.066
5.596IleGly: 5.596 ± 0.1
1.776IleHis: 1.776 ± 0.049
5.099IleIle: 5.099 ± 0.093
4.239IleLys: 4.239 ± 0.06
6.527IleLeu: 6.527 ± 0.104
2.041IleMet: 2.041 ± 0.046
3.064IleAsn: 3.064 ± 0.064
3.119IlePro: 3.119 ± 0.06
2.823IleGln: 2.823 ± 0.057
2.881IleArg: 2.881 ± 0.055
4.408IleSer: 4.408 ± 0.073
4.163IleThr: 4.163 ± 0.069
5.283IleVal: 5.283 ± 0.077
0.608IleTrp: 0.608 ± 0.025
2.278IleTyr: 2.278 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
4.74LysAla: 4.74 ± 0.072
0.301LysCys: 0.301 ± 0.023
3.819LysAsp: 3.819 ± 0.07
5.476LysGlu: 5.476 ± 0.099
1.527LysPhe: 1.527 ± 0.038
4.061LysGly: 4.061 ± 0.068
1.623LysHis: 1.623 ± 0.04
3.539LysIle: 3.539 ± 0.059
4.921LysLys: 4.921 ± 0.087
5.147LysLeu: 5.147 ± 0.073
1.943LysMet: 1.943 ± 0.046
2.76LysAsn: 2.76 ± 0.057
2.321LysPro: 2.321 ± 0.048
3.446LysGln: 3.446 ± 0.063
3.473LysArg: 3.473 ± 0.06
3.225LysSer: 3.225 ± 0.05
3.77LysThr: 3.77 ± 0.051
3.914LysVal: 3.914 ± 0.072
0.797LysTrp: 0.797 ± 0.028
1.773LysTyr: 1.773 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
7.244LeuAla: 7.244 ± 0.099
0.514LeuCys: 0.514 ± 0.025
5.275LeuAsp: 5.275 ± 0.076
6.385LeuGlu: 6.385 ± 0.098
4.685LeuPhe: 4.685 ± 0.096
6.402LeuGly: 6.402 ± 0.088
1.92LeuHis: 1.92 ± 0.047
7.011LeuIle: 7.011 ± 0.107
6.144LeuLys: 6.144 ± 0.084
9.362LeuLeu: 9.362 ± 0.136
2.696LeuMet: 2.696 ± 0.057
4.278LeuAsn: 4.278 ± 0.07
3.775LeuPro: 3.775 ± 0.06
3.335LeuGln: 3.335 ± 0.065
3.623LeuArg: 3.623 ± 0.064
6.299LeuSer: 6.299 ± 0.094
5.684LeuThr: 5.684 ± 0.072
6.125LeuVal: 6.125 ± 0.086
0.788LeuTrp: 0.788 ± 0.027
3.065LeuTyr: 3.065 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.371MetAla: 2.371 ± 0.05
0.163MetCys: 0.163 ± 0.013
1.749MetAsp: 1.749 ± 0.041
1.934MetGlu: 1.934 ± 0.044
1.164MetPhe: 1.164 ± 0.04
1.913MetGly: 1.913 ± 0.039
0.6MetHis: 0.6 ± 0.024
2.348MetIle: 2.348 ± 0.05
2.266MetLys: 2.266 ± 0.048
2.93MetLeu: 2.93 ± 0.059
0.961MetMet: 0.961 ± 0.031
1.622MetAsn: 1.622 ± 0.044
1.215MetPro: 1.215 ± 0.035
1.085MetGln: 1.085 ± 0.031
1.312MetArg: 1.312 ± 0.037
1.864MetSer: 1.864 ± 0.041
2.053MetThr: 2.053 ± 0.049
1.881MetVal: 1.881 ± 0.051
0.22MetTrp: 0.22 ± 0.016
0.839MetTyr: 0.839 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.963AsnAla: 2.963 ± 0.06
0.271AsnCys: 0.271 ± 0.018
2.577AsnAsp: 2.577 ± 0.062
3.181AsnGlu: 3.181 ± 0.061
1.41AsnPhe: 1.41 ± 0.041
3.038AsnGly: 3.038 ± 0.066
1.109AsnHis: 1.109 ± 0.035
3.034AsnIle: 3.034 ± 0.053
2.559AsnLys: 2.559 ± 0.043
3.325AsnLeu: 3.325 ± 0.049
1.336AsnMet: 1.336 ± 0.033
1.893AsnAsn: 1.893 ± 0.055
1.918AsnPro: 1.918 ± 0.047
1.89AsnGln: 1.89 ± 0.046
1.994AsnArg: 1.994 ± 0.044
1.917AsnSer: 1.917 ± 0.053
2.315AsnThr: 2.315 ± 0.052
2.917AsnVal: 2.917 ± 0.046
0.508AsnTrp: 0.508 ± 0.023
1.335AsnTyr: 1.335 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
2.54ProAla: 2.54 ± 0.054
0.199ProCys: 0.199 ± 0.013
2.554ProAsp: 2.554 ± 0.062
3.352ProGlu: 3.352 ± 0.065
1.938ProPhe: 1.938 ± 0.038
2.504ProGly: 2.504 ± 0.054
0.889ProHis: 0.889 ± 0.028
2.602ProIle: 2.602 ± 0.049
2.009ProLys: 2.009 ± 0.046
3.437ProLeu: 3.437 ± 0.064
0.93ProMet: 0.93 ± 0.03
1.572ProAsn: 1.572 ± 0.031
1.088ProPro: 1.088 ± 0.031
1.131ProGln: 1.131 ± 0.037
1.165ProArg: 1.165 ± 0.031
2.226ProSer: 2.226 ± 0.046
1.876ProThr: 1.876 ± 0.041
3.081ProVal: 3.081 ± 0.061
0.386ProTrp: 0.386 ± 0.021
1.462ProTyr: 1.462 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
3.283GlnAla: 3.283 ± 0.067
0.199GlnCys: 0.199 ± 0.014
2.093GlnAsp: 2.093 ± 0.045
2.995GlnGlu: 2.995 ± 0.063
1.621GlnPhe: 1.621 ± 0.042
2.171GlnGly: 2.171 ± 0.048
0.967GlnHis: 0.967 ± 0.035
2.547GlnIle: 2.547 ± 0.053
2.775GlnLys: 2.775 ± 0.055
4.018GlnLeu: 4.018 ± 0.076
1.299GlnMet: 1.299 ± 0.037
1.71GlnAsn: 1.71 ± 0.048
1.37GlnPro: 1.37 ± 0.034
1.87GlnGln: 1.87 ± 0.055
1.614GlnArg: 1.614 ± 0.044
2.244GlnSer: 2.244 ± 0.051
2.58GlnThr: 2.58 ± 0.052
2.429GlnVal: 2.429 ± 0.046
0.393GlnTrp: 0.393 ± 0.02
1.369GlnTyr: 1.369 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.674ArgAla: 2.674 ± 0.057
0.292ArgCys: 0.292 ± 0.017
2.317ArgAsp: 2.317 ± 0.053
3.187ArgGlu: 3.187 ± 0.062
2.005ArgPhe: 2.005 ± 0.044
2.455ArgGly: 2.455 ± 0.047
1.129ArgHis: 1.129 ± 0.034
3.003ArgIle: 3.003 ± 0.057
3.018ArgLys: 3.018 ± 0.06
4.097ArgLeu: 4.097 ± 0.066
1.526ArgMet: 1.526 ± 0.04
1.76ArgAsn: 1.76 ± 0.045
1.479ArgPro: 1.479 ± 0.042
1.853ArgGln: 1.853 ± 0.043
2.152ArgArg: 2.152 ± 0.054
2.305ArgSer: 2.305 ± 0.051
2.315ArgThr: 2.315 ± 0.046
2.857ArgVal: 2.857 ± 0.056
0.501ArgTrp: 0.501 ± 0.023
1.592ArgTyr: 1.592 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
3.971SerAla: 3.971 ± 0.063
0.359SerCys: 0.359 ± 0.019
3.247SerAsp: 3.247 ± 0.056
3.766SerGlu: 3.766 ± 0.063
2.797SerPhe: 2.797 ± 0.059
4.764SerGly: 4.764 ± 0.069
1.366SerHis: 1.366 ± 0.038
4.213SerIle: 4.213 ± 0.077
3.226SerLys: 3.226 ± 0.057
5.418SerLeu: 5.418 ± 0.081
1.828SerMet: 1.828 ± 0.041
2.186SerAsn: 2.186 ± 0.048
1.989SerPro: 1.989 ± 0.045
2.025SerGln: 2.025 ± 0.044
2.456SerArg: 2.456 ± 0.042
3.459SerSer: 3.459 ± 0.068
2.783SerThr: 2.783 ± 0.054
4.065SerVal: 4.065 ± 0.059
0.643SerTrp: 0.643 ± 0.027
2.147SerTyr: 2.147 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.434ThrAla: 4.434 ± 0.074
0.398ThrCys: 0.398 ± 0.02
3.268ThrAsp: 3.268 ± 0.062
3.776ThrGlu: 3.776 ± 0.068
2.794ThrPhe: 2.794 ± 0.057
4.434ThrGly: 4.434 ± 0.071
1.312ThrHis: 1.312 ± 0.038
4.552ThrIle: 4.552 ± 0.07
2.864ThrLys: 2.864 ± 0.052
5.469ThrLeu: 5.469 ± 0.079
1.514ThrMet: 1.514 ± 0.039
2.286ThrAsn: 2.286 ± 0.054
2.335ThrPro: 2.335 ± 0.048
1.792ThrGln: 1.792 ± 0.038
2.125ThrArg: 2.125 ± 0.05
3.289ThrSer: 3.289 ± 0.048
3.182ThrThr: 3.182 ± 0.071
4.331ThrVal: 4.331 ± 0.076
0.577ThrTrp: 0.577 ± 0.024
2.157ThrTyr: 2.157 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
5.113ValAla: 5.113 ± 0.072
0.542ValCys: 0.542 ± 0.022
4.064ValAsp: 4.064 ± 0.071
4.614ValGlu: 4.614 ± 0.08
3.299ValPhe: 3.299 ± 0.068
4.559ValGly: 4.559 ± 0.072
1.545ValHis: 1.545 ± 0.041
5.662ValIle: 5.662 ± 0.089
4.226ValLys: 4.226 ± 0.069
7.153ValLeu: 7.153 ± 0.096
2.15ValMet: 2.15 ± 0.055
2.936ValAsn: 2.936 ± 0.06
2.814ValPro: 2.814 ± 0.052
2.516ValGln: 2.516 ± 0.052
2.688ValArg: 2.688 ± 0.048
4.45ValSer: 4.45 ± 0.064
4.161ValThr: 4.161 ± 0.071
5.31ValVal: 5.31 ± 0.081
0.65ValTrp: 0.65 ± 0.026
2.389ValTyr: 2.389 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.669TrpAla: 0.669 ± 0.029
0.081TrpCys: 0.081 ± 0.008
0.564TrpAsp: 0.564 ± 0.026
0.681TrpGlu: 0.681 ± 0.025
0.544TrpPhe: 0.544 ± 0.025
0.664TrpGly: 0.664 ± 0.028
0.207TrpHis: 0.207 ± 0.015
0.785TrpIle: 0.785 ± 0.031
0.646TrpLys: 0.646 ± 0.03
1.195TrpLeu: 1.195 ± 0.037
0.378TrpMet: 0.378 ± 0.02
0.474TrpAsn: 0.474 ± 0.02
0.275TrpPro: 0.275 ± 0.016
0.443TrpGln: 0.443 ± 0.024
0.466TrpArg: 0.466 ± 0.023
0.595TrpSer: 0.595 ± 0.023
0.594TrpThr: 0.594 ± 0.027
0.708TrpVal: 0.708 ± 0.027
0.14TrpTrp: 0.14 ± 0.012
0.389TrpTyr: 0.389 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.371TyrAla: 2.371 ± 0.052
0.265TyrCys: 0.265 ± 0.019
2.207TyrAsp: 2.207 ± 0.055
2.598TyrGlu: 2.598 ± 0.053
1.706TyrPhe: 1.706 ± 0.043
2.495TyrGly: 2.495 ± 0.053
0.879TyrHis: 0.879 ± 0.032
2.425TyrIle: 2.425 ± 0.052
1.878TyrLys: 1.878 ± 0.047
3.133TyrLeu: 3.133 ± 0.058
0.994TyrMet: 0.994 ± 0.031
1.371TyrAsn: 1.371 ± 0.04
1.39TyrPro: 1.39 ± 0.037
1.557TyrGln: 1.557 ± 0.034
1.66TyrArg: 1.66 ± 0.046
1.733TyrSer: 1.733 ± 0.044
2.052TyrThr: 2.052 ± 0.053
2.305TyrVal: 2.305 ± 0.043
0.386TyrTrp: 0.386 ± 0.024
1.382TyrTyr: 1.382 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3784 proteins (1022484 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski