Amino acid dipepetide frequency for Streptococcus phage phiJH1301-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.273AlaAla: 1.273 ± 0.532
0.764AlaCys: 0.764 ± 0.52
5.601AlaAsp: 5.601 ± 1.25
4.328AlaGlu: 4.328 ± 1.159
1.273AlaPhe: 1.273 ± 0.476
3.564AlaGly: 3.564 ± 0.716
0.0AlaHis: 0.0 ± 0.0
6.619AlaIle: 6.619 ± 1.105
3.819AlaLys: 3.819 ± 0.943
6.365AlaLeu: 6.365 ± 0.749
2.291AlaMet: 2.291 ± 0.971
2.8AlaAsn: 2.8 ± 0.74
1.782AlaPro: 1.782 ± 0.632
2.8AlaGln: 2.8 ± 0.836
3.31AlaArg: 3.31 ± 0.635
4.328AlaSer: 4.328 ± 1.07
5.346AlaThr: 5.346 ± 1.859
3.055AlaVal: 3.055 ± 0.817
0.509AlaTrp: 0.509 ± 0.288
3.31AlaTyr: 3.31 ± 0.783
0.0AlaXaa: 0.0 ± 0.0
Cys
0.509CysAla: 0.509 ± 0.309
0.0CysCys: 0.0 ± 0.0
0.764CysAsp: 0.764 ± 0.409
0.255CysGlu: 0.255 ± 0.235
0.255CysPhe: 0.255 ± 0.235
0.509CysGly: 0.509 ± 0.316
0.0CysHis: 0.0 ± 0.0
1.018CysIle: 1.018 ± 0.408
0.0CysLys: 0.0 ± 0.0
0.255CysLeu: 0.255 ± 0.235
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.509CysPro: 0.509 ± 0.469
0.509CysGln: 0.509 ± 0.298
0.509CysArg: 0.509 ± 0.363
0.0CysSer: 0.0 ± 0.0
0.255CysThr: 0.255 ± 0.235
0.764CysVal: 0.764 ± 0.396
0.255CysTrp: 0.255 ± 0.282
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
0.764AspAla: 0.764 ± 0.367
0.509AspCys: 0.509 ± 0.347
4.073AspAsp: 4.073 ± 1.086
5.092AspGlu: 5.092 ± 1.184
3.31AspPhe: 3.31 ± 0.767
1.527AspGly: 1.527 ± 0.549
1.527AspHis: 1.527 ± 0.568
4.837AspIle: 4.837 ± 1.045
5.092AspLys: 5.092 ± 0.982
5.092AspLeu: 5.092 ± 1.168
2.037AspMet: 2.037 ± 0.676
2.291AspAsn: 2.291 ± 0.63
2.037AspPro: 2.037 ± 0.949
1.273AspGln: 1.273 ± 0.457
2.291AspArg: 2.291 ± 0.54
2.8AspSer: 2.8 ± 0.848
3.819AspThr: 3.819 ± 1.081
2.546AspVal: 2.546 ± 1.005
0.764AspTrp: 0.764 ± 0.391
4.837AspTyr: 4.837 ± 1.12
0.0AspXaa: 0.0 ± 0.0
Glu
6.619GluAla: 6.619 ± 1.082
0.255GluCys: 0.255 ± 0.263
6.11GluAsp: 6.11 ± 1.079
4.582GluGlu: 4.582 ± 1.443
2.8GluPhe: 2.8 ± 0.955
3.31GluGly: 3.31 ± 0.764
2.037GluHis: 2.037 ± 0.773
6.11GluIle: 6.11 ± 1.063
7.637GluLys: 7.637 ± 1.335
9.42GluLeu: 9.42 ± 2.003
2.291GluMet: 2.291 ± 0.964
3.055GluAsn: 3.055 ± 0.794
1.018GluPro: 1.018 ± 0.481
5.092GluGln: 5.092 ± 1.442
4.328GluArg: 4.328 ± 1.109
3.31GluSer: 3.31 ± 0.977
3.564GluThr: 3.564 ± 0.674
4.328GluVal: 4.328 ± 1.071
1.018GluTrp: 1.018 ± 0.623
4.073GluTyr: 4.073 ± 1.135
0.0GluXaa: 0.0 ± 0.0
Phe
1.527PheAla: 1.527 ± 0.57
0.255PheCys: 0.255 ± 0.259
3.055PheAsp: 3.055 ± 0.741
3.31PheGlu: 3.31 ± 0.825
0.509PhePhe: 0.509 ± 0.351
2.037PheGly: 2.037 ± 0.427
1.018PheHis: 1.018 ± 0.346
2.546PheIle: 2.546 ± 0.942
2.8PheLys: 2.8 ± 1.119
5.092PheLeu: 5.092 ± 0.641
1.273PheMet: 1.273 ± 0.54
2.546PheAsn: 2.546 ± 0.576
0.764PhePro: 0.764 ± 0.357
2.037PheGln: 2.037 ± 0.678
1.273PheArg: 1.273 ± 0.538
2.546PheSer: 2.546 ± 0.735
3.31PheThr: 3.31 ± 0.731
1.782PheVal: 1.782 ± 0.712
0.255PheTrp: 0.255 ± 0.213
3.31PheTyr: 3.31 ± 0.819
0.0PheXaa: 0.0 ± 0.0
Gly
4.073GlyAla: 4.073 ± 1.175
0.255GlyCys: 0.255 ± 0.243
2.8GlyAsp: 2.8 ± 0.998
2.291GlyGlu: 2.291 ± 0.663
3.819GlyPhe: 3.819 ± 0.743
1.527GlyGly: 1.527 ± 0.653
1.273GlyHis: 1.273 ± 0.553
3.31GlyIle: 3.31 ± 0.661
4.073GlyLys: 4.073 ± 0.926
6.365GlyLeu: 6.365 ± 1.092
2.037GlyMet: 2.037 ± 0.503
2.8GlyAsn: 2.8 ± 0.591
0.0GlyPro: 0.0 ± 0.0
2.546GlyGln: 2.546 ± 1.208
3.31GlyArg: 3.31 ± 0.865
1.018GlySer: 1.018 ± 0.418
2.546GlyThr: 2.546 ± 0.559
3.31GlyVal: 3.31 ± 0.663
2.037GlyTrp: 2.037 ± 0.746
2.546GlyTyr: 2.546 ± 0.69
0.0GlyXaa: 0.0 ± 0.0
His
2.291HisAla: 2.291 ± 0.83
0.255HisCys: 0.255 ± 0.213
1.273HisAsp: 1.273 ± 0.538
0.764HisGlu: 0.764 ± 0.394
2.291HisPhe: 2.291 ± 0.62
0.764HisGly: 0.764 ± 0.377
0.764HisHis: 0.764 ± 0.573
1.527HisIle: 1.527 ± 0.827
2.037HisLys: 2.037 ± 0.831
1.527HisLeu: 1.527 ± 0.685
0.255HisMet: 0.255 ± 0.224
0.764HisAsn: 0.764 ± 0.396
0.255HisPro: 0.255 ± 0.264
1.273HisGln: 1.273 ± 0.7
0.764HisArg: 0.764 ± 0.325
1.527HisSer: 1.527 ± 0.636
1.018HisThr: 1.018 ± 0.547
1.527HisVal: 1.527 ± 0.703
0.255HisTrp: 0.255 ± 0.243
1.018HisTyr: 1.018 ± 0.492
0.0HisXaa: 0.0 ± 0.0
Ile
5.092IleAla: 5.092 ± 1.1
0.509IleCys: 0.509 ± 0.309
5.092IleAsp: 5.092 ± 1.064
6.11IleGlu: 6.11 ± 1.257
4.328IlePhe: 4.328 ± 1.175
2.291IleGly: 2.291 ± 0.567
1.782IleHis: 1.782 ± 0.586
1.782IleIle: 1.782 ± 0.481
7.892IleLys: 7.892 ± 1.593
5.855IleLeu: 5.855 ± 1.138
1.018IleMet: 1.018 ± 0.447
3.055IleAsn: 3.055 ± 0.993
2.8IlePro: 2.8 ± 0.727
2.546IleGln: 2.546 ± 0.562
4.328IleArg: 4.328 ± 0.789
3.055IleSer: 3.055 ± 0.788
5.346IleThr: 5.346 ± 1.115
2.037IleVal: 2.037 ± 0.94
0.509IleTrp: 0.509 ± 0.308
2.291IleTyr: 2.291 ± 0.564
0.0IleXaa: 0.0 ± 0.0
Lys
4.837LysAla: 4.837 ± 1.007
0.509LysCys: 0.509 ± 0.368
4.073LysAsp: 4.073 ± 1.141
8.401LysGlu: 8.401 ± 1.449
1.782LysPhe: 1.782 ± 0.682
4.837LysGly: 4.837 ± 1.285
2.546LysHis: 2.546 ± 0.851
5.855LysIle: 5.855 ± 0.897
8.147LysLys: 8.147 ± 1.697
7.892LysLeu: 7.892 ± 1.345
1.273LysMet: 1.273 ± 0.521
4.582LysAsn: 4.582 ± 1.048
5.092LysPro: 5.092 ± 1.046
4.073LysGln: 4.073 ± 0.811
5.601LysArg: 5.601 ± 0.997
4.328LysSer: 4.328 ± 1.11
5.855LysThr: 5.855 ± 1.389
3.31LysVal: 3.31 ± 0.672
0.0LysTrp: 0.0 ± 0.0
2.037LysTyr: 2.037 ± 0.772
0.0LysXaa: 0.0 ± 0.0
Leu
8.401LeuAla: 8.401 ± 1.408
0.509LeuCys: 0.509 ± 0.469
6.11LeuAsp: 6.11 ± 0.972
10.183LeuGlu: 10.183 ± 1.717
4.073LeuPhe: 4.073 ± 0.883
5.346LeuGly: 5.346 ± 1.129
2.546LeuHis: 2.546 ± 0.645
5.346LeuIle: 5.346 ± 0.935
8.401LeuLys: 8.401 ± 1.424
7.637LeuLeu: 7.637 ± 1.337
1.782LeuMet: 1.782 ± 0.725
3.055LeuAsn: 3.055 ± 0.965
5.346LeuPro: 5.346 ± 1.003
6.11LeuGln: 6.11 ± 1.325
2.546LeuArg: 2.546 ± 0.781
3.564LeuSer: 3.564 ± 0.973
7.383LeuThr: 7.383 ± 0.943
4.073LeuVal: 4.073 ± 0.985
1.527LeuTrp: 1.527 ± 0.677
3.564LeuTyr: 3.564 ± 0.869
0.0LeuXaa: 0.0 ± 0.0
Met
2.291MetAla: 2.291 ± 0.687
0.0MetCys: 0.0 ± 0.0
1.782MetAsp: 1.782 ± 0.893
2.037MetGlu: 2.037 ± 0.513
0.255MetPhe: 0.255 ± 0.213
0.764MetGly: 0.764 ± 0.335
0.0MetHis: 0.0 ± 0.0
1.018MetIle: 1.018 ± 0.468
2.8MetLys: 2.8 ± 0.593
2.8MetLeu: 2.8 ± 0.798
0.509MetMet: 0.509 ± 0.396
1.527MetAsn: 1.527 ± 0.553
0.255MetPro: 0.255 ± 0.283
1.018MetGln: 1.018 ± 0.58
1.018MetArg: 1.018 ± 0.421
1.273MetSer: 1.273 ± 0.497
3.055MetThr: 3.055 ± 1.009
1.273MetVal: 1.273 ± 0.555
0.0MetTrp: 0.0 ± 0.0
0.255MetTyr: 0.255 ± 0.246
0.0MetXaa: 0.0 ± 0.0
Asn
2.8AsnAla: 2.8 ± 0.93
0.255AsnCys: 0.255 ± 0.235
1.018AsnAsp: 1.018 ± 0.559
2.037AsnGlu: 2.037 ± 0.764
1.273AsnPhe: 1.273 ± 0.58
4.837AsnGly: 4.837 ± 0.927
0.509AsnHis: 0.509 ± 0.296
2.291AsnIle: 2.291 ± 0.816
1.782AsnLys: 1.782 ± 0.884
4.328AsnLeu: 4.328 ± 0.889
2.291AsnMet: 2.291 ± 0.676
3.564AsnAsn: 3.564 ± 0.767
1.527AsnPro: 1.527 ± 0.659
2.8AsnGln: 2.8 ± 1.059
4.073AsnArg: 4.073 ± 0.672
1.782AsnSer: 1.782 ± 0.754
2.8AsnThr: 2.8 ± 1.176
2.291AsnVal: 2.291 ± 0.706
0.255AsnTrp: 0.255 ± 0.253
1.782AsnTyr: 1.782 ± 0.563
0.0AsnXaa: 0.0 ± 0.0
Pro
2.546ProAla: 2.546 ± 0.505
0.0ProCys: 0.0 ± 0.0
2.8ProAsp: 2.8 ± 0.757
3.819ProGlu: 3.819 ± 0.941
1.782ProPhe: 1.782 ± 0.786
0.764ProGly: 0.764 ± 0.376
0.509ProHis: 0.509 ± 0.296
0.764ProIle: 0.764 ± 0.343
2.037ProLys: 2.037 ± 0.442
4.073ProLeu: 4.073 ± 0.9
0.0ProMet: 0.0 ± 0.0
1.273ProAsn: 1.273 ± 0.719
0.509ProPro: 0.509 ± 0.306
0.764ProGln: 0.764 ± 0.343
2.546ProArg: 2.546 ± 0.73
2.037ProSer: 2.037 ± 0.673
2.291ProThr: 2.291 ± 0.647
2.546ProVal: 2.546 ± 0.999
0.0ProTrp: 0.0 ± 0.0
1.527ProTyr: 1.527 ± 0.487
0.0ProXaa: 0.0 ± 0.0
Gln
4.328GlnAla: 4.328 ± 0.809
0.509GlnCys: 0.509 ± 0.469
1.273GlnAsp: 1.273 ± 0.416
5.346GlnGlu: 5.346 ± 1.045
2.037GlnPhe: 2.037 ± 0.539
1.527GlnGly: 1.527 ± 0.563
1.782GlnHis: 1.782 ± 0.585
2.037GlnIle: 2.037 ± 0.861
3.819GlnLys: 3.819 ± 1.455
5.092GlnLeu: 5.092 ± 0.955
0.764GlnMet: 0.764 ± 0.354
1.018GlnAsn: 1.018 ± 0.446
0.509GlnPro: 0.509 ± 0.341
1.782GlnGln: 1.782 ± 0.611
2.037GlnArg: 2.037 ± 0.682
3.564GlnSer: 3.564 ± 0.811
1.527GlnThr: 1.527 ± 0.367
5.346GlnVal: 5.346 ± 0.989
0.0GlnTrp: 0.0 ± 0.0
2.037GlnTyr: 2.037 ± 0.733
0.0GlnXaa: 0.0 ± 0.0
Arg
1.782ArgAla: 1.782 ± 0.495
0.0ArgCys: 0.0 ± 0.0
1.018ArgAsp: 1.018 ± 0.588
4.328ArgGlu: 4.328 ± 1.049
2.037ArgPhe: 2.037 ± 0.536
3.31ArgGly: 3.31 ± 0.716
1.273ArgHis: 1.273 ± 0.481
2.8ArgIle: 2.8 ± 0.816
5.855ArgLys: 5.855 ± 1.082
6.619ArgLeu: 6.619 ± 1.02
1.273ArgMet: 1.273 ± 0.45
2.291ArgAsn: 2.291 ± 0.669
2.546ArgPro: 2.546 ± 0.961
2.8ArgGln: 2.8 ± 0.638
3.31ArgArg: 3.31 ± 0.821
1.527ArgSer: 1.527 ± 0.412
4.073ArgThr: 4.073 ± 1.446
3.819ArgVal: 3.819 ± 1.289
1.018ArgTrp: 1.018 ± 0.461
2.8ArgTyr: 2.8 ± 0.793
0.0ArgXaa: 0.0 ± 0.0
Ser
2.291SerAla: 2.291 ± 0.516
0.255SerCys: 0.255 ± 0.282
3.819SerAsp: 3.819 ± 0.797
5.855SerGlu: 5.855 ± 1.164
0.509SerPhe: 0.509 ± 0.327
3.564SerGly: 3.564 ± 0.979
1.527SerHis: 1.527 ± 0.514
5.601SerIle: 5.601 ± 1.025
4.328SerLys: 4.328 ± 0.774
4.582SerLeu: 4.582 ± 0.881
1.018SerMet: 1.018 ± 0.433
2.546SerAsn: 2.546 ± 0.6
0.764SerPro: 0.764 ± 0.339
3.564SerGln: 3.564 ± 0.747
1.527SerArg: 1.527 ± 0.486
2.037SerSer: 2.037 ± 0.603
2.037SerThr: 2.037 ± 0.576
3.31SerVal: 3.31 ± 0.8
0.255SerTrp: 0.255 ± 0.213
2.291SerTyr: 2.291 ± 0.76
0.0SerXaa: 0.0 ± 0.0
Thr
5.601ThrAla: 5.601 ± 1.284
0.255ThrCys: 0.255 ± 0.213
1.527ThrAsp: 1.527 ± 0.385
5.346ThrGlu: 5.346 ± 1.175
3.819ThrPhe: 3.819 ± 1.392
6.11ThrGly: 6.11 ± 1.43
1.018ThrHis: 1.018 ± 0.54
5.346ThrIle: 5.346 ± 1.037
2.8ThrLys: 2.8 ± 0.653
5.855ThrLeu: 5.855 ± 1.01
1.273ThrMet: 1.273 ± 0.583
1.018ThrAsn: 1.018 ± 0.495
3.055ThrPro: 3.055 ± 0.565
1.527ThrGln: 1.527 ± 0.54
4.582ThrArg: 4.582 ± 1.109
4.328ThrSer: 4.328 ± 1.081
5.855ThrThr: 5.855 ± 1.449
4.073ThrVal: 4.073 ± 1.15
0.509ThrTrp: 0.509 ± 0.372
2.291ThrTyr: 2.291 ± 0.759
0.0ThrXaa: 0.0 ± 0.0
Val
5.092ValAla: 5.092 ± 0.947
0.764ValCys: 0.764 ± 0.373
2.546ValAsp: 2.546 ± 0.869
4.582ValGlu: 4.582 ± 1.238
2.037ValPhe: 2.037 ± 0.635
2.037ValGly: 2.037 ± 0.625
0.509ValHis: 0.509 ± 0.344
5.346ValIle: 5.346 ± 1.002
4.582ValLys: 4.582 ± 1.22
3.564ValLeu: 3.564 ± 0.772
1.782ValMet: 1.782 ± 0.454
2.8ValAsn: 2.8 ± 0.779
1.273ValPro: 1.273 ± 0.723
1.527ValGln: 1.527 ± 0.563
1.527ValArg: 1.527 ± 0.613
3.819ValSer: 3.819 ± 1.193
3.564ValThr: 3.564 ± 0.783
2.037ValVal: 2.037 ± 0.468
0.764ValTrp: 0.764 ± 0.379
2.8ValTyr: 2.8 ± 0.881
0.0ValXaa: 0.0 ± 0.0
Trp
0.509TrpAla: 0.509 ± 0.296
0.0TrpCys: 0.0 ± 0.0
0.255TrpAsp: 0.255 ± 0.244
0.764TrpGlu: 0.764 ± 0.412
1.018TrpPhe: 1.018 ± 0.706
0.255TrpGly: 0.255 ± 0.282
0.255TrpHis: 0.255 ± 0.263
0.764TrpIle: 0.764 ± 0.452
1.018TrpLys: 1.018 ± 0.42
1.782TrpLeu: 1.782 ± 0.651
0.255TrpMet: 0.255 ± 0.263
0.509TrpAsn: 0.509 ± 0.333
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
1.018TrpArg: 1.018 ± 0.463
1.273TrpSer: 1.273 ± 0.585
0.509TrpThr: 0.509 ± 0.363
0.509TrpVal: 0.509 ± 0.361
0.255TrpTrp: 0.255 ± 0.243
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
0.509TyrAla: 0.509 ± 0.319
0.509TyrCys: 0.509 ± 0.375
1.527TyrAsp: 1.527 ± 0.511
1.527TyrGlu: 1.527 ± 0.633
2.037TyrPhe: 2.037 ± 0.611
3.055TyrGly: 3.055 ± 0.899
1.273TyrHis: 1.273 ± 0.689
2.8TyrIle: 2.8 ± 0.803
6.11TyrLys: 6.11 ± 1.363
3.31TyrLeu: 3.31 ± 0.815
0.255TyrMet: 0.255 ± 0.258
2.8TyrAsn: 2.8 ± 0.735
2.546TyrPro: 2.546 ± 0.732
2.291TyrGln: 2.291 ± 0.739
4.582TyrArg: 4.582 ± 0.81
3.564TyrSer: 3.564 ± 0.805
2.291TyrThr: 2.291 ± 0.745
1.018TyrVal: 1.018 ± 0.4
0.764TyrTrp: 0.764 ± 0.475
1.273TyrTyr: 1.273 ± 0.549
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 24 proteins (3929 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski