Lactococcus phage Tuc2009 (Lactococcus lactis bacteriophage Tuc2009)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9XJF2|Q9XJF2_BPTU2 YopX domain-containing protein OS=Lactococcus phage Tuc2009 OX=35241 PE=4 SV=1
MM1 pKa = 7.4IPKK4 pKa = 9.81LRR6 pKa = 11.84AWDD9 pKa = 3.75KK10 pKa = 9.78QDD12 pKa = 3.13EE13 pKa = 4.32RR14 pKa = 11.84MSYY17 pKa = 11.42GEE19 pKa = 4.23VEE21 pKa = 4.38YY22 pKa = 10.8FDD24 pKa = 6.18DD25 pKa = 4.71SINYY29 pKa = 9.6RR30 pKa = 11.84FDD32 pKa = 3.61HH33 pKa = 6.6FCTGADD39 pKa = 3.25EE40 pKa = 5.49DD41 pKa = 4.65VEE43 pKa = 4.95FMQSTGIKK51 pKa = 9.7DD52 pKa = 3.45KK53 pKa = 11.46NGVEE57 pKa = 4.03IYY59 pKa = 10.59EE60 pKa = 4.08GDD62 pKa = 3.51ILKK65 pKa = 10.44LHH67 pKa = 7.29AIFLAPDD74 pKa = 3.51DD75 pKa = 4.59KK76 pKa = 10.89IGYY79 pKa = 9.3LEE81 pKa = 4.08YY82 pKa = 10.51SPKK85 pKa = 10.14YY86 pKa = 9.23GYY88 pKa = 10.75SIICEE93 pKa = 4.12GNRR96 pKa = 11.84LYY98 pKa = 10.63RR99 pKa = 11.84QEE101 pKa = 3.99YY102 pKa = 6.99WASTNKK108 pKa = 10.17LNYY111 pKa = 9.39EE112 pKa = 4.38VIGNIYY118 pKa = 9.74EE119 pKa = 4.13NPEE122 pKa = 4.19LLRR125 pKa = 11.84EE126 pKa = 4.13DD127 pKa = 4.2TKK129 pKa = 11.77NDD131 pKa = 3.17

Molecular weight: 15.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AYW4|Q9AYW4_BPTU2 Major structural protein 4 (Fragment) OS=Lactococcus phage Tuc2009 OX=35241 PE=4 SV=1
MM1 pKa = 7.25GRR3 pKa = 11.84LLSRR7 pKa = 11.84HH8 pKa = 4.0LHH10 pKa = 5.66KK11 pKa = 10.97YY12 pKa = 10.19KK13 pKa = 10.77NINATKK19 pKa = 9.75QVKK22 pKa = 9.96NDD24 pKa = 3.37EE25 pKa = 4.4LTTLTVNQLKK35 pKa = 10.27EE36 pKa = 3.84LLEE39 pKa = 4.42TKK41 pKa = 10.5GIEE44 pKa = 3.87YY45 pKa = 8.84TKK47 pKa = 10.91NDD49 pKa = 3.69KK50 pKa = 11.17KK51 pKa = 11.1EE52 pKa = 3.93DD53 pKa = 4.38LISKK57 pKa = 10.27LGVAYY62 pKa = 9.83GYY64 pKa = 10.8HH65 pKa = 6.47LL66 pKa = 4.36

Molecular weight: 7.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11748

32

1025

209.8

23.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.767 ± 0.45

0.604 ± 0.125

6.078 ± 0.199

7.159 ± 0.551

4.265 ± 0.216

6.597 ± 0.409

1.175 ± 0.149

6.801 ± 0.25

9.176 ± 0.44

7.823 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.135

6.171 ± 0.214

2.996 ± 0.199

4.256 ± 0.229

3.43 ± 0.289

6.682 ± 0.35

6.12 ± 0.36

5.899 ± 0.283

1.498 ± 0.204

3.933 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski