Amino acid dipepetide frequency for Lactococcus phage Tuc2009 (Lactococcus lactis bacteriophage Tuc2009)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.682AlaAla: 4.682 ± 1.183
0.341AlaCys: 0.341 ± 0.166
3.831AlaAsp: 3.831 ± 0.646
4.427AlaGlu: 4.427 ± 0.736
2.809AlaPhe: 2.809 ± 0.472
3.065AlaGly: 3.065 ± 0.493
0.851AlaHis: 0.851 ± 0.251
4.852AlaIle: 4.852 ± 0.931
6.64AlaLys: 6.64 ± 0.801
5.704AlaLeu: 5.704 ± 0.743
2.043AlaMet: 2.043 ± 0.359
5.363AlaAsn: 5.363 ± 0.738
1.873AlaPro: 1.873 ± 0.399
2.639AlaGln: 2.639 ± 0.424
1.788AlaArg: 1.788 ± 0.476
4.937AlaSer: 4.937 ± 0.704
4.512AlaThr: 4.512 ± 0.691
3.49AlaVal: 3.49 ± 0.594
1.362AlaTrp: 1.362 ± 0.322
2.469AlaTyr: 2.469 ± 0.49
0.0AlaXaa: 0.0 ± 0.0
Cys
0.426CysAla: 0.426 ± 0.19
0.085CysCys: 0.085 ± 0.076
0.511CysAsp: 0.511 ± 0.218
0.596CysGlu: 0.596 ± 0.223
0.17CysPhe: 0.17 ± 0.136
0.596CysGly: 0.596 ± 0.325
0.17CysHis: 0.17 ± 0.109
0.255CysIle: 0.255 ± 0.149
0.255CysLys: 0.255 ± 0.118
0.426CysLeu: 0.426 ± 0.191
0.17CysMet: 0.17 ± 0.134
0.255CysAsn: 0.255 ± 0.151
0.341CysPro: 0.341 ± 0.193
0.17CysGln: 0.17 ± 0.121
0.426CysArg: 0.426 ± 0.195
0.426CysSer: 0.426 ± 0.18
0.255CysThr: 0.255 ± 0.154
0.255CysVal: 0.255 ± 0.134
0.0CysTrp: 0.0 ± 0.0
0.255CysTyr: 0.255 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
2.979AspAla: 2.979 ± 0.604
0.511AspCys: 0.511 ± 0.237
4.171AspAsp: 4.171 ± 0.702
5.193AspGlu: 5.193 ± 0.76
3.405AspPhe: 3.405 ± 0.515
4.937AspGly: 4.937 ± 0.665
0.596AspHis: 0.596 ± 0.222
4.086AspIle: 4.086 ± 0.595
5.959AspLys: 5.959 ± 0.773
4.682AspLeu: 4.682 ± 0.555
1.022AspMet: 1.022 ± 0.326
3.916AspAsn: 3.916 ± 0.656
1.447AspPro: 1.447 ± 0.514
1.022AspGln: 1.022 ± 0.316
1.617AspArg: 1.617 ± 0.355
3.746AspSer: 3.746 ± 0.525
3.661AspThr: 3.661 ± 0.474
4.001AspVal: 4.001 ± 0.531
1.447AspTrp: 1.447 ± 0.306
2.298AspTyr: 2.298 ± 0.42
0.0AspXaa: 0.0 ± 0.0
Glu
3.661GluAla: 3.661 ± 0.488
0.511GluCys: 0.511 ± 0.215
3.065GluAsp: 3.065 ± 0.477
5.363GluGlu: 5.363 ± 1.119
3.575GluPhe: 3.575 ± 0.551
3.235GluGly: 3.235 ± 0.542
0.766GluHis: 0.766 ± 0.314
5.108GluIle: 5.108 ± 0.836
5.959GluLys: 5.959 ± 1.275
7.832GluLeu: 7.832 ± 1.016
2.554GluMet: 2.554 ± 0.448
3.916GluAsn: 3.916 ± 0.469
1.958GluPro: 1.958 ± 0.387
3.831GluGln: 3.831 ± 0.587
2.639GluArg: 2.639 ± 0.555
4.256GluSer: 4.256 ± 0.471
3.49GluThr: 3.49 ± 0.653
5.533GluVal: 5.533 ± 0.639
0.766GluTrp: 0.766 ± 0.271
3.575GluTyr: 3.575 ± 0.56
0.0GluXaa: 0.0 ± 0.0
Phe
2.809PheAla: 2.809 ± 0.451
0.511PheCys: 0.511 ± 0.249
4.001PheAsp: 4.001 ± 0.53
2.469PheGlu: 2.469 ± 0.473
1.703PhePhe: 1.703 ± 0.373
2.894PheGly: 2.894 ± 0.51
0.596PheHis: 0.596 ± 0.201
2.724PheIle: 2.724 ± 0.548
4.342PheLys: 4.342 ± 0.793
2.043PheLeu: 2.043 ± 0.371
1.192PheMet: 1.192 ± 0.324
2.298PheAsn: 2.298 ± 0.401
0.766PhePro: 0.766 ± 0.261
2.043PheGln: 2.043 ± 0.441
1.107PheArg: 1.107 ± 0.25
3.15PheSer: 3.15 ± 0.485
3.065PheThr: 3.065 ± 0.431
2.979PheVal: 2.979 ± 0.536
0.426PheTrp: 0.426 ± 0.211
1.532PheTyr: 1.532 ± 0.472
0.0PheXaa: 0.0 ± 0.0
Gly
4.001GlyAla: 4.001 ± 0.639
0.341GlyCys: 0.341 ± 0.184
3.575GlyAsp: 3.575 ± 0.653
4.427GlyGlu: 4.427 ± 0.677
3.405GlyPhe: 3.405 ± 0.579
4.682GlyGly: 4.682 ± 0.723
1.022GlyHis: 1.022 ± 0.368
5.108GlyIle: 5.108 ± 0.756
6.044GlyLys: 6.044 ± 0.838
5.618GlyLeu: 5.618 ± 1.071
1.617GlyMet: 1.617 ± 0.552
3.661GlyAsn: 3.661 ± 0.686
0.936GlyPro: 0.936 ± 0.373
2.724GlyGln: 2.724 ± 0.651
2.639GlyArg: 2.639 ± 0.58
4.086GlySer: 4.086 ± 0.729
5.023GlyThr: 5.023 ± 0.755
2.724GlyVal: 2.724 ± 0.492
0.936GlyTrp: 0.936 ± 0.354
3.405GlyTyr: 3.405 ± 0.618
0.0GlyXaa: 0.0 ± 0.0
His
1.447HisAla: 1.447 ± 0.418
0.085HisCys: 0.085 ± 0.083
0.936HisAsp: 0.936 ± 0.254
1.022HisGlu: 1.022 ± 0.343
0.936HisPhe: 0.936 ± 0.35
0.766HisGly: 0.766 ± 0.223
0.0HisHis: 0.0 ± 0.0
0.511HisIle: 0.511 ± 0.219
0.766HisLys: 0.766 ± 0.24
0.681HisLeu: 0.681 ± 0.282
0.0HisMet: 0.0 ± 0.0
0.511HisAsn: 0.511 ± 0.226
0.596HisPro: 0.596 ± 0.209
0.766HisGln: 0.766 ± 0.262
0.255HisArg: 0.255 ± 0.136
0.596HisSer: 0.596 ± 0.224
0.766HisThr: 0.766 ± 0.297
0.511HisVal: 0.511 ± 0.169
0.085HisTrp: 0.085 ± 0.086
0.511HisTyr: 0.511 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
4.682IleAla: 4.682 ± 0.625
0.511IleCys: 0.511 ± 0.222
3.916IleAsp: 3.916 ± 0.514
5.789IleGlu: 5.789 ± 0.835
1.788IlePhe: 1.788 ± 0.361
5.363IleGly: 5.363 ± 0.806
0.681IleHis: 0.681 ± 0.222
3.575IleIle: 3.575 ± 0.607
6.129IleLys: 6.129 ± 0.711
3.661IleLeu: 3.661 ± 0.569
1.958IleMet: 1.958 ± 0.277
4.767IleAsn: 4.767 ± 0.722
2.554IlePro: 2.554 ± 0.544
3.065IleGln: 3.065 ± 0.496
2.724IleArg: 2.724 ± 0.546
5.704IleSer: 5.704 ± 0.778
4.256IleThr: 4.256 ± 0.668
4.427IleVal: 4.427 ± 0.902
0.851IleTrp: 0.851 ± 0.207
1.617IleTyr: 1.617 ± 0.463
0.0IleXaa: 0.0 ± 0.0
Lys
7.321LysAla: 7.321 ± 1.072
0.426LysCys: 0.426 ± 0.191
6.044LysAsp: 6.044 ± 0.626
7.406LysGlu: 7.406 ± 1.137
3.916LysPhe: 3.916 ± 0.651
6.044LysGly: 6.044 ± 0.72
1.703LysHis: 1.703 ± 0.546
6.044LysIle: 6.044 ± 0.814
7.832LysLys: 7.832 ± 1.213
7.406LysLeu: 7.406 ± 1.008
1.958LysMet: 1.958 ± 0.411
5.789LysAsn: 5.789 ± 0.848
3.32LysPro: 3.32 ± 0.53
4.427LysGln: 4.427 ± 0.801
4.001LysArg: 4.001 ± 0.641
4.171LysSer: 4.171 ± 0.54
4.597LysThr: 4.597 ± 0.661
4.767LysVal: 4.767 ± 0.651
0.851LysTrp: 0.851 ± 0.254
3.746LysTyr: 3.746 ± 0.614
0.0LysXaa: 0.0 ± 0.0
Leu
5.193LeuAla: 5.193 ± 0.695
0.596LeuCys: 0.596 ± 0.256
5.278LeuAsp: 5.278 ± 0.549
5.533LeuGlu: 5.533 ± 0.768
2.128LeuPhe: 2.128 ± 0.516
4.937LeuGly: 4.937 ± 0.592
0.596LeuHis: 0.596 ± 0.213
5.704LeuIle: 5.704 ± 0.638
7.491LeuLys: 7.491 ± 1.071
7.151LeuLeu: 7.151 ± 0.795
2.128LeuMet: 2.128 ± 0.481
4.512LeuAsn: 4.512 ± 0.602
2.894LeuPro: 2.894 ± 0.404
3.235LeuGln: 3.235 ± 0.618
2.639LeuArg: 2.639 ± 0.508
6.81LeuSer: 6.81 ± 0.608
5.023LeuThr: 5.023 ± 0.697
2.639LeuVal: 2.639 ± 0.414
1.277LeuTrp: 1.277 ± 0.602
2.469LeuTyr: 2.469 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
2.384MetAla: 2.384 ± 0.415
0.085MetCys: 0.085 ± 0.075
1.107MetAsp: 1.107 ± 0.296
1.617MetGlu: 1.617 ± 0.396
0.851MetPhe: 0.851 ± 0.254
1.362MetGly: 1.362 ± 0.406
0.255MetHis: 0.255 ± 0.157
1.277MetIle: 1.277 ± 0.322
2.554MetLys: 2.554 ± 0.561
1.362MetLeu: 1.362 ± 0.336
0.936MetMet: 0.936 ± 0.234
1.788MetAsn: 1.788 ± 0.295
1.192MetPro: 1.192 ± 0.298
1.192MetGln: 1.192 ± 0.285
1.022MetArg: 1.022 ± 0.256
1.788MetSer: 1.788 ± 0.31
2.469MetThr: 2.469 ± 0.472
1.362MetVal: 1.362 ± 0.354
0.255MetTrp: 0.255 ± 0.125
0.851MetTyr: 0.851 ± 0.324
0.0MetXaa: 0.0 ± 0.0
Asn
5.108AsnAla: 5.108 ± 0.71
0.255AsnCys: 0.255 ± 0.133
3.235AsnAsp: 3.235 ± 0.553
2.894AsnGlu: 2.894 ± 0.612
2.384AsnPhe: 2.384 ± 0.599
6.555AsnGly: 6.555 ± 0.968
0.511AsnHis: 0.511 ± 0.294
3.49AsnIle: 3.49 ± 0.499
5.789AsnLys: 5.789 ± 0.744
5.789AsnLeu: 5.789 ± 0.898
1.703AsnMet: 1.703 ± 0.39
3.065AsnAsn: 3.065 ± 0.554
2.128AsnPro: 2.128 ± 0.425
2.639AsnGln: 2.639 ± 0.482
2.043AsnArg: 2.043 ± 0.417
4.597AsnSer: 4.597 ± 0.557
2.384AsnThr: 2.384 ± 0.369
3.32AsnVal: 3.32 ± 0.485
1.107AsnTrp: 1.107 ± 0.288
2.724AsnTyr: 2.724 ± 0.539
0.0AsnXaa: 0.0 ± 0.0
Pro
1.532ProAla: 1.532 ± 0.373
0.085ProCys: 0.085 ± 0.092
1.788ProAsp: 1.788 ± 0.426
2.213ProGlu: 2.213 ± 0.383
1.532ProPhe: 1.532 ± 0.404
0.766ProGly: 0.766 ± 0.248
0.426ProHis: 0.426 ± 0.172
2.384ProIle: 2.384 ± 0.452
3.575ProLys: 3.575 ± 0.549
2.384ProLeu: 2.384 ± 0.401
0.681ProMet: 0.681 ± 0.2
2.043ProAsn: 2.043 ± 0.503
0.681ProPro: 0.681 ± 0.193
1.788ProGln: 1.788 ± 0.499
0.766ProArg: 0.766 ± 0.311
1.958ProSer: 1.958 ± 0.547
1.703ProThr: 1.703 ± 0.382
1.958ProVal: 1.958 ± 0.429
0.17ProTrp: 0.17 ± 0.138
1.532ProTyr: 1.532 ± 0.343
0.0ProXaa: 0.0 ± 0.0
Gln
5.023GlnAla: 5.023 ± 0.566
0.085GlnCys: 0.085 ± 0.086
1.277GlnAsp: 1.277 ± 0.335
3.575GlnGlu: 3.575 ± 0.487
1.532GlnPhe: 1.532 ± 0.371
3.065GlnGly: 3.065 ± 0.496
0.255GlnHis: 0.255 ± 0.14
2.384GlnIle: 2.384 ± 0.38
3.831GlnLys: 3.831 ± 0.566
3.065GlnLeu: 3.065 ± 0.502
1.192GlnMet: 1.192 ± 0.278
2.469GlnAsn: 2.469 ± 0.454
1.532GlnPro: 1.532 ± 0.466
2.213GlnGln: 2.213 ± 0.479
1.107GlnArg: 1.107 ± 0.237
2.128GlnSer: 2.128 ± 0.49
2.894GlnThr: 2.894 ± 0.409
2.639GlnVal: 2.639 ± 0.4
1.192GlnTrp: 1.192 ± 0.459
1.107GlnTyr: 1.107 ± 0.243
0.0GlnXaa: 0.0 ± 0.0
Arg
2.213ArgAla: 2.213 ± 0.433
0.341ArgCys: 0.341 ± 0.166
1.958ArgAsp: 1.958 ± 0.287
2.384ArgGlu: 2.384 ± 0.45
2.043ArgPhe: 2.043 ± 0.38
1.277ArgGly: 1.277 ± 0.334
0.426ArgHis: 0.426 ± 0.198
2.809ArgIle: 2.809 ± 0.576
3.746ArgLys: 3.746 ± 0.726
3.49ArgLeu: 3.49 ± 0.635
0.936ArgMet: 0.936 ± 0.282
2.213ArgAsn: 2.213 ± 0.48
0.851ArgPro: 0.851 ± 0.428
1.362ArgGln: 1.362 ± 0.325
1.277ArgArg: 1.277 ± 0.363
1.703ArgSer: 1.703 ± 0.366
1.617ArgThr: 1.617 ± 0.39
1.788ArgVal: 1.788 ± 0.349
0.255ArgTrp: 0.255 ± 0.171
1.617ArgTyr: 1.617 ± 0.454
0.0ArgXaa: 0.0 ± 0.0
Ser
4.256SerAla: 4.256 ± 0.99
0.426SerCys: 0.426 ± 0.213
5.108SerAsp: 5.108 ± 0.619
4.767SerGlu: 4.767 ± 0.637
3.661SerPhe: 3.661 ± 0.779
5.533SerGly: 5.533 ± 1.02
0.596SerHis: 0.596 ± 0.235
4.767SerIle: 4.767 ± 0.534
4.256SerLys: 4.256 ± 0.588
4.171SerLeu: 4.171 ± 0.726
1.447SerMet: 1.447 ± 0.323
4.937SerAsn: 4.937 ± 0.955
1.192SerPro: 1.192 ± 0.309
2.724SerGln: 2.724 ± 0.481
1.873SerArg: 1.873 ± 0.321
4.767SerSer: 4.767 ± 0.768
3.831SerThr: 3.831 ± 0.453
4.767SerVal: 4.767 ± 0.468
0.766SerTrp: 0.766 ± 0.249
2.979SerTyr: 2.979 ± 0.452
0.0SerXaa: 0.0 ± 0.0
Thr
4.171ThrAla: 4.171 ± 0.637
0.085ThrCys: 0.085 ± 0.079
3.575ThrAsp: 3.575 ± 0.64
3.916ThrGlu: 3.916 ± 0.727
2.894ThrPhe: 2.894 ± 0.461
4.342ThrGly: 4.342 ± 0.686
0.426ThrHis: 0.426 ± 0.228
5.533ThrIle: 5.533 ± 0.794
5.193ThrLys: 5.193 ± 0.734
4.086ThrLeu: 4.086 ± 0.462
1.362ThrMet: 1.362 ± 0.377
2.639ThrAsn: 2.639 ± 0.437
2.213ThrPro: 2.213 ± 0.457
1.788ThrGln: 1.788 ± 0.316
2.128ThrArg: 2.128 ± 0.433
3.49ThrSer: 3.49 ± 0.47
4.597ThrThr: 4.597 ± 0.554
4.767ThrVal: 4.767 ± 0.768
1.277ThrTrp: 1.277 ± 0.423
2.724ThrTyr: 2.724 ± 0.559
0.0ThrXaa: 0.0 ± 0.0
Val
2.724ValAla: 2.724 ± 0.545
0.341ValCys: 0.341 ± 0.136
4.171ValAsp: 4.171 ± 0.808
4.597ValGlu: 4.597 ± 0.707
2.213ValPhe: 2.213 ± 0.432
3.065ValGly: 3.065 ± 0.511
0.851ValHis: 0.851 ± 0.286
3.575ValIle: 3.575 ± 0.432
6.725ValLys: 6.725 ± 0.865
4.767ValLeu: 4.767 ± 0.544
1.192ValMet: 1.192 ± 0.305
4.256ValAsn: 4.256 ± 0.616
1.617ValPro: 1.617 ± 0.397
2.043ValGln: 2.043 ± 0.54
1.617ValArg: 1.617 ± 0.355
5.023ValSer: 5.023 ± 0.591
3.831ValThr: 3.831 ± 0.487
3.831ValVal: 3.831 ± 0.603
0.681ValTrp: 0.681 ± 0.215
1.873ValTyr: 1.873 ± 0.481
0.0ValXaa: 0.0 ± 0.0
Trp
1.192TrpAla: 1.192 ± 0.3
0.0TrpCys: 0.0 ± 0.0
1.107TrpAsp: 1.107 ± 0.271
0.681TrpGlu: 0.681 ± 0.259
0.511TrpPhe: 0.511 ± 0.167
1.192TrpGly: 1.192 ± 0.27
0.255TrpHis: 0.255 ± 0.137
1.192TrpIle: 1.192 ± 0.24
1.022TrpLys: 1.022 ± 0.325
0.851TrpLeu: 0.851 ± 0.255
0.341TrpMet: 0.341 ± 0.157
1.107TrpAsn: 1.107 ± 0.558
0.255TrpPro: 0.255 ± 0.127
1.192TrpGln: 1.192 ± 0.341
0.596TrpArg: 0.596 ± 0.207
0.426TrpSer: 0.426 ± 0.199
0.766TrpThr: 0.766 ± 0.259
1.362TrpVal: 1.362 ± 0.478
0.341TrpTrp: 0.341 ± 0.185
0.596TrpTyr: 0.596 ± 0.255
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.873TyrAla: 1.873 ± 0.457
0.426TyrCys: 0.426 ± 0.185
2.128TyrAsp: 2.128 ± 0.373
2.724TyrGlu: 2.724 ± 0.564
1.192TyrPhe: 1.192 ± 0.298
2.298TyrGly: 2.298 ± 0.603
0.766TyrHis: 0.766 ± 0.29
2.639TyrIle: 2.639 ± 0.493
3.661TyrLys: 3.661 ± 0.534
3.065TyrLeu: 3.065 ± 0.711
1.192TyrMet: 1.192 ± 0.275
2.213TyrAsn: 2.213 ± 0.578
1.617TyrPro: 1.617 ± 0.314
1.703TyrGln: 1.703 ± 0.422
2.043TyrArg: 2.043 ± 0.423
3.065TyrSer: 3.065 ± 0.466
2.469TyrThr: 2.469 ± 0.546
1.873TyrVal: 1.873 ± 0.336
0.936TyrTrp: 0.936 ± 0.264
1.447TyrTyr: 1.447 ± 0.33
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11748 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski