Dishui Lake virophage 4

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XNE5|A0A6G6XNE5_9VIRU RING-type domain-containing protein OS=Dishui Lake virophage 4 OX=2704065 PE=4 SV=1
MM1 pKa = 7.75SFGTFWGTDD10 pKa = 3.42PTDD13 pKa = 2.87VATYY17 pKa = 10.29QKK19 pKa = 10.78ILGKK23 pKa = 10.0YY24 pKa = 9.65LEE26 pKa = 4.93GGAAAVPPLNSVLGVGNSAANQSATDD52 pKa = 4.42FNTLGCIEE60 pKa = 4.42IEE62 pKa = 4.17TGKK65 pKa = 10.69VYY67 pKa = 10.6QGNNLDD73 pKa = 4.03LEE75 pKa = 4.51IGEE78 pKa = 4.64VGDD81 pKa = 3.58TLKK84 pKa = 10.84ILGAITKK91 pKa = 10.39GSILVGNGVEE101 pKa = 4.47TKK103 pKa = 9.82EE104 pKa = 4.51LPVGANGLVLKK115 pKa = 10.26ANSGAAYY122 pKa = 9.32GVEE125 pKa = 3.98WGTDD129 pKa = 3.23ASGGTVMAVNAGTNISVSGTIAQPIVNFATPTTSNIVLGVGTEE172 pKa = 3.98IEE174 pKa = 4.31ATNGATTMTLDD185 pKa = 3.33ATGLNDD191 pKa = 3.69TYY193 pKa = 11.67SSGGVVNQEE202 pKa = 4.28DD203 pKa = 4.02IEE205 pKa = 4.63VNATSVVQLLSATDD219 pKa = 3.37GGSYY223 pKa = 10.98LNTDD227 pKa = 3.5TTNVSNTGIVEE238 pKa = 4.11NLSATNLSTNNIGNVSLTCFSNSAGIACGCSAPTTPPYY276 pKa = 10.56PEE278 pKa = 4.09VSATAGLSATTTNAQLTISQSAPFAISYY306 pKa = 7.55STTLDD311 pKa = 3.18INGITQNNTGGVAGFTINTNTQPLALTTGDD341 pKa = 4.78HH342 pKa = 5.95ITFSADD348 pKa = 4.38NIDD351 pKa = 3.92LDD353 pKa = 3.74ATGRR357 pKa = 11.84LVLPSLASGDD367 pKa = 3.84YY368 pKa = 10.74LDD370 pKa = 5.15YY371 pKa = 11.34NAGSLKK377 pKa = 10.15IVNDD381 pKa = 3.89SVGGSANPLLVLQNNNNTAGATTFEE406 pKa = 4.78TYY408 pKa = 10.85KK409 pKa = 10.65NDD411 pKa = 3.34QPTSTGGDD419 pKa = 3.72NIASWSATCNTNVGKK434 pKa = 8.64TEE436 pKa = 3.72ISRR439 pKa = 11.84VNHH442 pKa = 5.78IAYY445 pKa = 7.64GTGASNNDD453 pKa = 3.12GGISLACKK461 pKa = 9.9VNSSITNFLICNGGANNAIGSGEE484 pKa = 4.09VQVFKK489 pKa = 10.54PITSTSPNNLNLICPTAGGTAKK511 pKa = 9.97IQSVSNIEE519 pKa = 4.38LEE521 pKa = 4.19ATGDD525 pKa = 3.61NLTTISGANTTISTTGVGSQIEE547 pKa = 4.52FKK549 pKa = 11.0PEE551 pKa = 3.31TTAGKK556 pKa = 9.98IVFTGASLQSNTSSGNSGEE575 pKa = 4.2HH576 pKa = 5.67LVIYY580 pKa = 10.46LNGVKK585 pKa = 9.75YY586 pKa = 10.24HH587 pKa = 7.04IALQTPP593 pKa = 4.14

Molecular weight: 60.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XMQ5|A0A6G6XMQ5_9VIRU Uncharacterized protein OS=Dishui Lake virophage 4 OX=2704065 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 4.68RR3 pKa = 11.84RR4 pKa = 11.84FIFRR8 pKa = 11.84NYY10 pKa = 8.17KK11 pKa = 6.84TAKK14 pKa = 10.0KK15 pKa = 9.37YY16 pKa = 11.2GEE18 pKa = 4.52TIVPIPKK25 pKa = 9.47EE26 pKa = 3.76LHH28 pKa = 7.16DD29 pKa = 3.67ILAVYY34 pKa = 9.42FEE36 pKa = 4.68KK37 pKa = 10.72KK38 pKa = 10.39GILRR42 pKa = 11.84RR43 pKa = 11.84LQAPVKK49 pKa = 8.73KK50 pKa = 8.29TKK52 pKa = 10.36KK53 pKa = 8.32EE54 pKa = 3.52ASIFIEE60 pKa = 4.44PFLTLWNDD68 pKa = 3.03KK69 pKa = 10.34PFLINTITRR78 pKa = 11.84ILNRR82 pKa = 11.84VFGKK86 pKa = 10.66KK87 pKa = 9.32IGSSMLRR94 pKa = 11.84HH95 pKa = 6.69IYY97 pKa = 6.2TTKK100 pKa = 10.92KK101 pKa = 10.15FGKK104 pKa = 9.61QLAEE108 pKa = 3.92QKK110 pKa = 8.5EE111 pKa = 4.28TAEE114 pKa = 4.06QMGHH118 pKa = 5.79SVAEE122 pKa = 4.14MNQTYY127 pKa = 10.18IKK129 pKa = 10.52EE130 pKa = 4.17DD131 pKa = 3.15

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

4887

114

963

375.9

41.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.469 ± 0.48

1.166 ± 0.188

5.463 ± 0.342

6.384 ± 0.814

3.458 ± 0.298

6.834 ± 0.735

1.228 ± 0.135

6.528 ± 0.547

7.285 ± 1.034

8.615 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.304

6.384 ± 0.568

4.931 ± 0.572

3.397 ± 0.22

4.87 ± 0.72

6.65 ± 0.924

6.077 ± 0.955

5.77 ± 0.241

1.187 ± 0.201

3.888 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski