Natranaerobius trueperi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Natranaerobiales; Natranaerobiaceae; Natranaerobius

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2464 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A226BXL2|A0A226BXL2_9FIRM Group II intron reverse transcriptase/maturase (Fragment) OS=Natranaerobius trueperi OX=759412 GN=CDO51_11720 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 9.17RR3 pKa = 11.84TSLYY7 pKa = 10.76LVLSLFLVIAGAMAVTGCDD26 pKa = 2.98EE27 pKa = 4.24SEE29 pKa = 4.43RR30 pKa = 11.84YY31 pKa = 9.77AIATGGSGGVYY42 pKa = 9.95YY43 pKa = 10.12PLGTAMSNILNDD55 pKa = 3.51EE56 pKa = 4.33LEE58 pKa = 4.46GVDD61 pKa = 4.69ISAEE65 pKa = 4.08STGASVEE72 pKa = 4.26NVNLTANEE80 pKa = 4.17DD81 pKa = 3.4VHH83 pKa = 7.51LALVQNDD90 pKa = 3.01ISYY93 pKa = 10.26YY94 pKa = 8.7AYY96 pKa = 10.23EE97 pKa = 4.82GIEE100 pKa = 4.06MFDD103 pKa = 3.91DD104 pKa = 4.14EE105 pKa = 5.3EE106 pKa = 4.99PMEE109 pKa = 4.43EE110 pKa = 3.9LRR112 pKa = 11.84GMGTLYY118 pKa = 10.39PEE120 pKa = 5.25TIQIVADD127 pKa = 3.67AGEE130 pKa = 4.8DD131 pKa = 3.45IEE133 pKa = 4.86TVEE136 pKa = 4.7DD137 pKa = 3.97LDD139 pKa = 4.06GKK141 pKa = 10.23RR142 pKa = 11.84VAVGAPGSGTEE153 pKa = 3.86ANARR157 pKa = 11.84QLLNAHH163 pKa = 7.48GITYY167 pKa = 10.75DD168 pKa = 5.16DD169 pKa = 4.3IEE171 pKa = 6.07ADD173 pKa = 3.51YY174 pKa = 11.4LSFSEE179 pKa = 5.51ASDD182 pKa = 3.6NLRR185 pKa = 11.84DD186 pKa = 3.7GHH188 pKa = 7.99LDD190 pKa = 3.32AAFVTAGTPTASITDD205 pKa = 4.14LSTTHH210 pKa = 6.72NIDD213 pKa = 3.19LVSISEE219 pKa = 4.11EE220 pKa = 3.68MSEE223 pKa = 4.89EE224 pKa = 3.87IQEE227 pKa = 4.33DD228 pKa = 4.32YY229 pKa = 10.76PYY231 pKa = 10.11YY232 pKa = 11.07AEE234 pKa = 4.06VTIDD238 pKa = 3.13EE239 pKa = 4.66GTYY242 pKa = 10.61SDD244 pKa = 3.99DD245 pKa = 4.03QEE247 pKa = 4.39DD248 pKa = 3.91VRR250 pKa = 11.84ALAVMAMIIVHH261 pKa = 6.95EE262 pKa = 5.05DD263 pKa = 3.18LPEE266 pKa = 5.94DD267 pKa = 3.32IVYY270 pKa = 10.71EE271 pKa = 4.03MTKK274 pKa = 10.78GIFTSTDD281 pKa = 3.17EE282 pKa = 4.57LGEE285 pKa = 3.92AHH287 pKa = 6.17EE288 pKa = 4.93RR289 pKa = 11.84GYY291 pKa = 11.24EE292 pKa = 4.07VTAEE296 pKa = 4.0DD297 pKa = 4.15ALEE300 pKa = 4.32GMSLEE305 pKa = 4.12LHH307 pKa = 6.66LGAEE311 pKa = 4.32RR312 pKa = 11.84FFDD315 pKa = 3.84EE316 pKa = 4.59QQ317 pKa = 3.43

Molecular weight: 34.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A226BWZ8|A0A226BWZ8_9FIRM Peptidase_M23 domain-containing protein OS=Natranaerobius trueperi OX=759412 GN=CDO51_08275 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.69KK9 pKa = 7.88RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.23TKK25 pKa = 10.17AGRR28 pKa = 11.84NILRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.15GRR39 pKa = 11.84KK40 pKa = 9.03SLTAA44 pKa = 4.07

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2464

0

2464

713096

26

2179

289.4

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.588 ± 0.05

0.882 ± 0.018

5.861 ± 0.053

8.2 ± 0.068

4.225 ± 0.042

6.742 ± 0.053

1.73 ± 0.02

8.411 ± 0.045

7.688 ± 0.057

9.906 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.023

5.217 ± 0.038

3.452 ± 0.027

3.203 ± 0.031

3.84 ± 0.036

6.234 ± 0.038

5.292 ± 0.033

6.864 ± 0.042

0.742 ± 0.017

3.592 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski