Halonotius sp. F13-13

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halonotius; unclassified Halonotius

Average proteome isoelectric point is 4.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2460 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A6PSN4|A0A3A6PSN4_9EURY Uncharacterized protein OS=Halonotius sp. F13-13 OX=2216978 GN=DM826_12615 PE=4 SV=1
MM1 pKa = 7.46TISDD5 pKa = 3.67RR6 pKa = 11.84VVFDD10 pKa = 4.21AEE12 pKa = 4.24PLVAHH17 pKa = 7.24ADD19 pKa = 3.81DD20 pKa = 4.57EE21 pKa = 5.0PGSEE25 pKa = 4.22VVEE28 pKa = 5.02DD29 pKa = 3.94YY30 pKa = 11.47LDD32 pKa = 3.96AVAADD37 pKa = 3.91DD38 pKa = 3.71TTGYY42 pKa = 10.45VSYY45 pKa = 9.83VTLTEE50 pKa = 3.51VRR52 pKa = 11.84YY53 pKa = 9.89ILSQKK58 pKa = 9.24YY59 pKa = 10.12DD60 pKa = 3.1RR61 pKa = 11.84GTADD65 pKa = 5.12EE66 pKa = 4.7YY67 pKa = 11.07IDD69 pKa = 3.58WLTDD73 pKa = 3.49LGIEE77 pKa = 4.14PVGVADD83 pKa = 4.54CWQGTADD90 pKa = 4.44HH91 pKa = 6.37VLSHH95 pKa = 6.42NPALGDD101 pKa = 3.66AFALATADD109 pKa = 4.28HH110 pKa = 7.21LDD112 pKa = 3.28ATLLVGADD120 pKa = 3.96DD121 pKa = 6.2DD122 pKa = 4.64YY123 pKa = 12.06DD124 pKa = 4.16AVDD127 pKa = 4.23DD128 pKa = 4.23VSITRR133 pKa = 11.84FRR135 pKa = 11.84NEE137 pKa = 3.42AGG139 pKa = 3.28

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A6PUN0|A0A3A6PUN0_9EURY Uncharacterized protein OS=Halonotius sp. F13-13 OX=2216978 GN=DM826_10195 PE=4 SV=1
MM1 pKa = 7.22VVGLVVTAFWAMLPAYY17 pKa = 9.57VPNNAAVLAGGGRR30 pKa = 11.84PIDD33 pKa = 4.31GGRR36 pKa = 11.84TWGDD40 pKa = 2.84RR41 pKa = 11.84RR42 pKa = 11.84LLGDD46 pKa = 3.85GKK48 pKa = 7.53TWRR51 pKa = 11.84GTAVGTLVGITLAAGLNVAAAPLGSAIGFSLPTFPPAGALGLAAGAMAGDD101 pKa = 4.12IAASFVKK108 pKa = 10.14RR109 pKa = 11.84RR110 pKa = 11.84SGRR113 pKa = 11.84ARR115 pKa = 11.84GAAFPGLDD123 pKa = 3.01QLDD126 pKa = 4.07FVVGAILFAFVLAPGWAQATFTLGVLAVIVVVTPVLHH163 pKa = 6.23VGTNIIAYY171 pKa = 10.09LIGAKK176 pKa = 10.04NEE178 pKa = 3.87PWW180 pKa = 3.37

Molecular weight: 18.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2460

0

2460

717023

10

1850

291.5

31.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.184 ± 0.077

0.7 ± 0.015

8.847 ± 0.07

8.205 ± 0.07

3.177 ± 0.033

8.082 ± 0.053

1.956 ± 0.025

4.901 ± 0.043

1.937 ± 0.033

8.84 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.703 ± 0.022

2.472 ± 0.028

4.611 ± 0.033

2.921 ± 0.03

5.834 ± 0.039

5.549 ± 0.036

7.094 ± 0.042

8.352 ± 0.054

1.041 ± 0.018

2.595 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski