Candidatus Syntrophosphaera thermopropionivorans

Taxonomy: cellular organisms; Bacteria; FCB group; Candidatus Cloacimonetes; Candidatus Syntrophosphaera

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5JQB6|A0A4R5JQB6_9BACT Glucose 1-dehydrogenase OS=Candidatus Syntrophosphaera thermopropionivorans OX=2593015 GN=E0946_04425 PE=4 SV=1
MM1 pKa = 7.42NKK3 pKa = 9.96YY4 pKa = 10.63LLLLLLLPVMIFAQPWQQDD23 pKa = 3.21NAIFNPSGIPSLPFSQPRR41 pKa = 11.84FVDD44 pKa = 3.94LDD46 pKa = 3.66ADD48 pKa = 3.59GDD50 pKa = 3.82MDD52 pKa = 5.0FFLGNTNSAPLYY64 pKa = 8.76ICNIGTATSPHH75 pKa = 5.94FVIGDD80 pKa = 3.59NLLANISFIDD90 pKa = 4.03AEE92 pKa = 4.41LAVCADD98 pKa = 3.62MNADD102 pKa = 3.69GKK104 pKa = 11.28LDD106 pKa = 5.62LITGGYY112 pKa = 7.81TGLHH116 pKa = 6.26LFLNTGTIQSPVYY129 pKa = 9.67TEE131 pKa = 3.57QLGYY135 pKa = 10.69FSTLEE140 pKa = 3.94VGNNPVPDD148 pKa = 3.73VADD151 pKa = 3.33VDD153 pKa = 4.14GDD155 pKa = 3.86GDD157 pKa = 4.37LDD159 pKa = 4.07LVVGLSEE166 pKa = 4.68DD167 pKa = 3.76GAVRR171 pKa = 11.84VYY173 pKa = 10.89FNTGTASNGIFQEE186 pKa = 4.43EE187 pKa = 4.69SMQLIGDD194 pKa = 4.04VGLYY198 pKa = 9.81AYY200 pKa = 9.47PVFCDD205 pKa = 4.05LDD207 pKa = 3.71NDD209 pKa = 3.99GDD211 pKa = 3.9QDD213 pKa = 3.88ILCGRR218 pKa = 11.84DD219 pKa = 2.93AHH221 pKa = 6.7GFVYY225 pKa = 10.2YY226 pKa = 10.43QNNGTISSPLWEE238 pKa = 4.57NNSALFSGLGNSTYY252 pKa = 10.19WNSPDD257 pKa = 5.67LVDD260 pKa = 5.92LDD262 pKa = 4.19GDD264 pKa = 4.04GDD266 pKa = 3.93FDD268 pKa = 6.91LIFGTADD275 pKa = 3.43GPLKK279 pKa = 10.82YY280 pKa = 10.07YY281 pKa = 10.73VNNGTPSSPQWQEE294 pKa = 3.39NTNLFGGVIDD304 pKa = 4.77AGGASSPVLFDD315 pKa = 3.97FDD317 pKa = 4.9GDD319 pKa = 3.68GDD321 pKa = 4.64LDD323 pKa = 5.05LICGTQLGDD332 pKa = 2.84IKK334 pKa = 10.66FYY336 pKa = 11.43KK337 pKa = 10.72NIGNPYY343 pKa = 8.97APAWQEE349 pKa = 3.65DD350 pKa = 3.39SSYY353 pKa = 10.82FSSIDD358 pKa = 3.18HH359 pKa = 6.81SIYY362 pKa = 10.79SAITLGDD369 pKa = 3.21VDD371 pKa = 6.04ADD373 pKa = 3.97GLPDD377 pKa = 5.54AIVGDD382 pKa = 4.2LSGNLYY388 pKa = 9.45FHH390 pKa = 7.47KK391 pKa = 10.41NTGTGFIQVNNVLPPISVGGWSVPRR416 pKa = 11.84LIDD419 pKa = 3.4MDD421 pKa = 4.43SDD423 pKa = 3.91GDD425 pKa = 3.78LDD427 pKa = 4.07LAVGNEE433 pKa = 3.88AGNIYY438 pKa = 10.35YY439 pKa = 9.27YY440 pKa = 10.76QNNGSPEE447 pKa = 4.1LPQWTLVSGFFGNIDD462 pKa = 3.58VGSDD466 pKa = 3.41CSPTFGDD473 pKa = 3.42LDD475 pKa = 3.97EE476 pKa = 5.03NGRR479 pKa = 11.84LDD481 pKa = 5.15LLAGDD486 pKa = 5.05LFGSLHH492 pKa = 6.79CYY494 pKa = 10.34LYY496 pKa = 10.82QNHH499 pKa = 5.7NWVNNTEE506 pKa = 4.07LFAGIEE512 pKa = 4.42TNQNAAPALADD523 pKa = 4.2LDD525 pKa = 4.19HH526 pKa = 7.53DD527 pKa = 4.84GDD529 pKa = 4.47LDD531 pKa = 4.81LVLGDD536 pKa = 3.76YY537 pKa = 11.23DD538 pKa = 4.0GTFKK542 pKa = 10.72FYY544 pKa = 11.17RR545 pKa = 11.84NLKK548 pKa = 9.51YY549 pKa = 10.79SPAVLNPPLNLLANTDD565 pKa = 3.84EE566 pKa = 4.64PVTISWEE573 pKa = 4.19EE574 pKa = 4.02PQGSTSPLEE583 pKa = 4.25HH584 pKa = 6.55YY585 pKa = 10.63NIYY588 pKa = 10.52LDD590 pKa = 3.96NEE592 pKa = 4.58FVDD595 pKa = 3.83STTFTFYY602 pKa = 11.46SFFNWDD608 pKa = 3.13FTVAHH613 pKa = 6.32IVKK616 pKa = 8.4VTAQYY621 pKa = 10.91VAGEE625 pKa = 4.27SVPAIIEE632 pKa = 4.1IPATPLNDD640 pKa = 3.47EE641 pKa = 4.28YY642 pKa = 11.56QSIGIISIYY651 pKa = 9.32PNPVTDD657 pKa = 3.27ITNINLTLKK666 pKa = 10.63SEE668 pKa = 4.56DD669 pKa = 3.45KK670 pKa = 10.64ATVSIYY676 pKa = 10.15NLKK679 pKa = 9.41GQQIIGWEE687 pKa = 4.31VVSKK691 pKa = 11.08GNHH694 pKa = 4.33NLIWNGKK701 pKa = 8.15DD702 pKa = 3.32KK703 pKa = 10.96QGKK706 pKa = 7.67PVANGIYY713 pKa = 9.89LLNVKK718 pKa = 8.72TPQNTITRR726 pKa = 11.84KK727 pKa = 9.58ISLIKK732 pKa = 10.61

Molecular weight: 79.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5JRB7|A0A4R5JRB7_9BACT Uncharacterized protein OS=Candidatus Syntrophosphaera thermopropionivorans OX=2593015 GN=E0946_02790 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.3QPSNRR10 pKa = 11.84SRR12 pKa = 11.84KK13 pKa = 7.31STHH16 pKa = 5.08GFRR19 pKa = 11.84SRR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 5.15TLCVV44 pKa = 3.33

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1369

0

1369

524344

38

2085

383.0

43.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.423 ± 0.053

1.065 ± 0.021

5.272 ± 0.041

6.7 ± 0.078

4.392 ± 0.038

6.096 ± 0.057

1.844 ± 0.026

8.503 ± 0.056

6.338 ± 0.076

10.125 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.03

5.613 ± 0.07

4.467 ± 0.038

3.845 ± 0.037

3.996 ± 0.047

6.31 ± 0.059

5.38 ± 0.072

5.643 ± 0.051

1.164 ± 0.028

4.556 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski