Amino acid dipepetide frequency for Eubacterium plexicaudatum ASF492

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.458AlaAla: 7.458 ± 0.089	1.177AlaCys: 1.177 ± 0.029	5.016AlaAsp: 5.016 ± 0.051	5.477AlaGlu: 5.477 ± 0.068	3.085AlaPhe: 3.085 ± 0.042	5.84AlaGly: 5.84 ± 0.066	1.182AlaHis: 1.182 ± 0.029	4.25AlaIle: 4.25 ± 0.055	4.727AlaLys: 4.727 ± 0.059	6.401AlaLeu: 6.401 ± 0.061	2.221AlaMet: 2.221 ± 0.039	2.533AlaAsn: 2.533 ± 0.036	1.791AlaPro: 1.791 ± 0.042	2.78AlaGln: 2.78 ± 0.043	2.923AlaArg: 2.923 ± 0.044	3.853AlaSer: 3.853 ± 0.045	2.681AlaThr: 2.681 ± 0.05	6.386AlaVal: 6.386 ± 0.068	0.72AlaTrp: 0.72 ± 0.021	3.014AlaTyr: 3.014 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.132CysAla: 1.132 ± 0.026	0.324CysCys: 0.324 ± 0.014	0.864CysAsp: 0.864 ± 0.023	0.99CysGlu: 0.99 ± 0.022	0.755CysPhe: 0.755 ± 0.019	1.541CysGly: 1.541 ± 0.034	0.322CysHis: 0.322 ± 0.015	1.263CysIle: 1.263 ± 0.026	0.967CysLys: 0.967 ± 0.027	1.314CysLeu: 1.314 ± 0.03	0.636CysMet: 0.636 ± 0.021	0.63CysAsn: 0.63 ± 0.019	0.656CysPro: 0.656 ± 0.023	0.48CysGln: 0.48 ± 0.017	0.984CysArg: 0.984 ± 0.024	0.978CysSer: 0.978 ± 0.025	0.801CysThr: 0.801 ± 0.02	1.037CysVal: 1.037 ± 0.025	0.139CysTrp: 0.139 ± 0.009	0.674CysTyr: 0.674 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.33AspAla: 4.33 ± 0.062	0.948AspCys: 0.948 ± 0.024	3.041AspAsp: 3.041 ± 0.043	4.269AspGlu: 4.269 ± 0.057	2.768AspPhe: 2.768 ± 0.038	4.348AspGly: 4.348 ± 0.058	0.973AspHis: 0.973 ± 0.023	4.591AspIle: 4.591 ± 0.057	3.46AspLys: 3.46 ± 0.044	4.428AspLeu: 4.428 ± 0.051	1.86AspMet: 1.86 ± 0.027	2.242AspAsn: 2.242 ± 0.039	1.525AspPro: 1.525 ± 0.033	1.474AspGln: 1.474 ± 0.03	2.869AspArg: 2.869 ± 0.042	3.201AspSer: 3.201 ± 0.04	3.267AspThr: 3.267 ± 0.047	3.506AspVal: 3.506 ± 0.048	0.624AspTrp: 0.624 ± 0.019	2.895AspTyr: 2.895 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	5.029GluAla: 5.029 ± 0.056	0.954GluCys: 0.954 ± 0.024	3.975GluAsp: 3.975 ± 0.046	6.318GluGlu: 6.318 ± 0.069	2.557GluPhe: 2.557 ± 0.04	3.772GluGly: 3.772 ± 0.051	1.561GluHis: 1.561 ± 0.033	5.528GluIle: 5.528 ± 0.063	6.864GluLys: 6.864 ± 0.077	6.844GluLeu: 6.844 ± 0.074	2.355GluMet: 2.355 ± 0.043	4.174GluAsn: 4.174 ± 0.053	1.924GluPro: 1.924 ± 0.037	3.883GluGln: 3.883 ± 0.062	3.836GluArg: 3.836 ± 0.053	3.438GluSer: 3.438 ± 0.048	3.803GluThr: 3.803 ± 0.049	3.984GluVal: 3.984 ± 0.05	0.756GluTrp: 0.756 ± 0.021	3.295GluTyr: 3.295 ± 0.049	0.001GluXaa: 0.001 ± 0.001
Phe	3.055PheAla: 3.055 ± 0.048	0.903PheCys: 0.903 ± 0.026	2.54PheAsp: 2.54 ± 0.039	2.694PheGlu: 2.694 ± 0.041	1.909PhePhe: 1.909 ± 0.043	2.804PheGly: 2.804 ± 0.043	0.972PheHis: 0.972 ± 0.026	2.834PheIle: 2.834 ± 0.045	1.854PheLys: 1.854 ± 0.036	4.017PheLeu: 4.017 ± 0.057	1.201PheMet: 1.201 ± 0.026	1.43PheAsn: 1.43 ± 0.03	1.293PhePro: 1.293 ± 0.027	1.566PheGln: 1.566 ± 0.033	1.956PheArg: 1.956 ± 0.031	2.954PheSer: 2.954 ± 0.051	2.236PheThr: 2.236 ± 0.04	2.752PheVal: 2.752 ± 0.045	0.489PheTrp: 0.489 ± 0.017	1.998PheTyr: 1.998 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.329GlyAla: 4.329 ± 0.066	1.241GlyCys: 1.241 ± 0.026	3.115GlyAsp: 3.115 ± 0.045	4.111GlyGlu: 4.111 ± 0.051	2.929GlyPhe: 2.929 ± 0.042	4.061GlyGly: 4.061 ± 0.064	1.182GlyHis: 1.182 ± 0.031	5.778GlyIle: 5.778 ± 0.064	5.264GlyLys: 5.264 ± 0.056	5.168GlyLeu: 5.168 ± 0.055	2.552GlyMet: 2.552 ± 0.039	3.188GlyAsn: 3.188 ± 0.049	1.024GlyPro: 1.024 ± 0.041	2.215GlyGln: 2.215 ± 0.034	3.296GlyArg: 3.296 ± 0.057	3.959GlySer: 3.959 ± 0.058	3.927GlyThr: 3.927 ± 0.061	4.053GlyVal: 4.053 ± 0.053	0.714GlyTrp: 0.714 ± 0.022	3.265GlyTyr: 3.265 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.396HisAla: 1.396 ± 0.031	0.33HisCys: 0.33 ± 0.014	0.994HisAsp: 0.994 ± 0.027	1.19HisGlu: 1.19 ± 0.026	0.883HisPhe: 0.883 ± 0.024	1.304HisGly: 1.304 ± 0.027	0.448HisHis: 0.448 ± 0.016	1.588HisIle: 1.588 ± 0.027	1.109HisLys: 1.109 ± 0.028	1.643HisLeu: 1.643 ± 0.036	0.656HisMet: 0.656 ± 0.02	0.798HisAsn: 0.798 ± 0.025	0.869HisPro: 0.869 ± 0.024	0.619HisGln: 0.619 ± 0.02	0.904HisArg: 0.904 ± 0.024	1.056HisSer: 1.056 ± 0.025	1.177HisThr: 1.177 ± 0.03	1.154HisVal: 1.154 ± 0.025	0.183HisTrp: 0.183 ± 0.011	0.853HisTyr: 0.853 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.319IleAla: 5.319 ± 0.056	1.489IleCys: 1.489 ± 0.029	4.004IleAsp: 4.004 ± 0.045	4.716IleGlu: 4.716 ± 0.06	2.97IlePhe: 2.97 ± 0.05	4.808IleGly: 4.808 ± 0.056	1.489IleHis: 1.489 ± 0.029	4.644IleIle: 4.644 ± 0.073	4.06IleLys: 4.06 ± 0.057	6.827IleLeu: 6.827 ± 0.08	1.948IleMet: 1.948 ± 0.032	2.86IleAsn: 2.86 ± 0.044	2.863IlePro: 2.863 ± 0.043	2.43IleGln: 2.43 ± 0.037	4.411IleArg: 4.411 ± 0.051	4.9IleSer: 4.9 ± 0.073	3.987IleThr: 3.987 ± 0.06	4.602IleVal: 4.602 ± 0.053	0.693IleTrp: 0.693 ± 0.019	3.017IleTyr: 3.017 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	4.75LysAla: 4.75 ± 0.048	0.851LysCys: 0.851 ± 0.021	3.888LysAsp: 3.888 ± 0.044	6.615LysGlu: 6.615 ± 0.079	1.882LysPhe: 1.882 ± 0.031	3.801LysGly: 3.801 ± 0.046	1.16LysHis: 1.16 ± 0.027	5.098LysIle: 5.098 ± 0.057	6.535LysLys: 6.535 ± 0.076	5.451LysLeu: 5.451 ± 0.061	2.196LysMet: 2.196 ± 0.036	3.997LysAsn: 3.997 ± 0.049	2.048LysPro: 2.048 ± 0.034	3.023LysGln: 3.023 ± 0.047	3.474LysArg: 3.474 ± 0.05	3.563LysSer: 3.563 ± 0.048	3.81LysThr: 3.81 ± 0.049	3.808LysVal: 3.808 ± 0.051	0.64LysTrp: 0.64 ± 0.018	3.178LysTyr: 3.178 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	6.597LeuAla: 6.597 ± 0.066	1.703LeuCys: 1.703 ± 0.032	4.79LeuAsp: 4.79 ± 0.053	6.399LeuGlu: 6.399 ± 0.07	3.927LeuPhe: 3.927 ± 0.056	4.983LeuGly: 4.983 ± 0.061	1.847LeuHis: 1.847 ± 0.035	5.584LeuIle: 5.584 ± 0.062	6.341LeuLys: 6.341 ± 0.068	8.914LeuLeu: 8.914 ± 0.09	2.559LeuMet: 2.559 ± 0.037	3.899LeuAsn: 3.899 ± 0.048	3.356LeuPro: 3.356 ± 0.048	3.273LeuGln: 3.273 ± 0.048	4.014LeuArg: 4.014 ± 0.051	6.266LeuSer: 6.266 ± 0.072	4.92LeuThr: 4.92 ± 0.061	4.868LeuVal: 4.868 ± 0.054	0.833LeuTrp: 0.833 ± 0.024	3.663LeuTyr: 3.663 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.362MetAla: 2.362 ± 0.042	0.408MetCys: 0.408 ± 0.014	1.975MetAsp: 1.975 ± 0.039	2.884MetGlu: 2.884 ± 0.045	1.044MetPhe: 1.044 ± 0.023	1.916MetGly: 1.916 ± 0.033	0.577MetHis: 0.577 ± 0.019	2.22MetIle: 2.22 ± 0.033	2.457MetLys: 2.457 ± 0.036	2.901MetLeu: 2.901 ± 0.047	0.956MetMet: 0.956 ± 0.027	1.599MetAsn: 1.599 ± 0.029	1.156MetPro: 1.156 ± 0.028	1.367MetGln: 1.367 ± 0.032	1.537MetArg: 1.537 ± 0.035	1.747MetSer: 1.747 ± 0.03	1.65MetThr: 1.65 ± 0.031	1.77MetVal: 1.77 ± 0.032	0.243MetTrp: 0.243 ± 0.012	1.041MetTyr: 1.041 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.284AsnAla: 3.284 ± 0.046	0.663AsnCys: 0.663 ± 0.019	2.173AsnAsp: 2.173 ± 0.038	2.963AsnGlu: 2.963 ± 0.048	1.642AsnPhe: 1.642 ± 0.032	3.566AsnGly: 3.566 ± 0.063	0.915AsnHis: 0.915 ± 0.024	3.657AsnIle: 3.657 ± 0.049	2.681AsnLys: 2.681 ± 0.042	3.799AsnLeu: 3.799 ± 0.05	1.466AsnMet: 1.466 ± 0.03	1.933AsnAsn: 1.933 ± 0.046	1.808AsnPro: 1.808 ± 0.037	1.612AsnGln: 1.612 ± 0.037	2.406AsnArg: 2.406 ± 0.038	2.384AsnSer: 2.384 ± 0.039	2.301AsnThr: 2.301 ± 0.037	2.726AsnVal: 2.726 ± 0.045	0.405AsnTrp: 0.405 ± 0.014	1.991AsnTyr: 1.991 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	2.282ProAla: 2.282 ± 0.045	0.467ProCys: 0.467 ± 0.016	2.479ProAsp: 2.479 ± 0.041	2.905ProGlu: 2.905 ± 0.045	1.441ProPhe: 1.441 ± 0.033	1.864ProGly: 1.864 ± 0.037	0.578ProHis: 0.578 ± 0.018	1.907ProIle: 1.907 ± 0.038	1.882ProLys: 1.882 ± 0.029	2.576ProLeu: 2.576 ± 0.037	0.839ProMet: 0.839 ± 0.021	1.161ProAsn: 1.161 ± 0.029	0.766ProPro: 0.766 ± 0.023	1.367ProGln: 1.367 ± 0.032	0.889ProArg: 0.889 ± 0.023	1.661ProSer: 1.661 ± 0.031	1.268ProThr: 1.268 ± 0.036	2.83ProVal: 2.83 ± 0.043	0.319ProTrp: 0.319 ± 0.015	1.461ProTyr: 1.461 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	2.945GlnAla: 2.945 ± 0.048	0.441GlnCys: 0.441 ± 0.015	1.867GlnAsp: 1.867 ± 0.032	3.351GlnGlu: 3.351 ± 0.052	1.349GlnPhe: 1.349 ± 0.025	2.001GlnGly: 2.001 ± 0.037	0.573GlnHis: 0.573 ± 0.018	2.985GlnIle: 2.985 ± 0.042	3.203GlnLys: 3.203 ± 0.049	3.336GlnLeu: 3.336 ± 0.041	1.488GlnMet: 1.488 ± 0.03	1.876GlnAsn: 1.876 ± 0.034	1.097GlnPro: 1.097 ± 0.025	1.674GlnGln: 1.674 ± 0.039	1.672GlnArg: 1.672 ± 0.035	1.975GlnSer: 1.975 ± 0.04	2.121GlnThr: 2.121 ± 0.036	2.164GlnVal: 2.164 ± 0.036	0.363GlnTrp: 0.363 ± 0.016	1.607GlnTyr: 1.607 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.777ArgAla: 2.777 ± 0.04	0.716ArgCys: 0.716 ± 0.019	2.301ArgAsp: 2.301 ± 0.032	3.883ArgGlu: 3.883 ± 0.057	2.191ArgPhe: 2.191 ± 0.035	2.336ArgGly: 2.336 ± 0.035	0.978ArgHis: 0.978 ± 0.028	3.884ArgIle: 3.884 ± 0.053	4.114ArgLys: 4.114 ± 0.053	4.573ArgLeu: 4.573 ± 0.053	1.978ArgMet: 1.978 ± 0.036	2.346ArgAsn: 2.346 ± 0.036	1.378ArgPro: 1.378 ± 0.031	2.375ArgGln: 2.375 ± 0.037	2.674ArgArg: 2.674 ± 0.048	2.501ArgSer: 2.501 ± 0.038	2.55ArgThr: 2.55 ± 0.038	2.509ArgVal: 2.509 ± 0.037	0.479ArgTrp: 0.479 ± 0.015	2.309ArgTyr: 2.309 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.265SerAla: 4.265 ± 0.06	0.925SerCys: 0.925 ± 0.022	3.524SerAsp: 3.524 ± 0.049	4.013SerGlu: 4.013 ± 0.046	2.669SerPhe: 2.669 ± 0.044	4.939SerGly: 4.939 ± 0.071	1.094SerHis: 1.094 ± 0.027	4.089SerIle: 4.089 ± 0.061	3.457SerLys: 3.457 ± 0.043	4.911SerLeu: 4.911 ± 0.052	1.875SerMet: 1.875 ± 0.032	2.381SerAsn: 2.381 ± 0.048	1.573SerPro: 1.573 ± 0.028	1.96SerGln: 1.96 ± 0.038	2.735SerArg: 2.735 ± 0.046	3.335SerSer: 3.335 ± 0.063	2.491SerThr: 2.491 ± 0.039	4.436SerVal: 4.436 ± 0.052	0.551SerTrp: 0.551 ± 0.015	2.578SerTyr: 2.578 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.553ThrAla: 4.553 ± 0.064	0.677ThrCys: 0.677 ± 0.021	3.435ThrAsp: 3.435 ± 0.053	3.861ThrGlu: 3.861 ± 0.05	2.0ThrPhe: 2.0 ± 0.034	4.405ThrGly: 4.405 ± 0.07	0.861ThrHis: 0.861 ± 0.023	3.765ThrIle: 3.765 ± 0.053	3.061ThrLys: 3.061 ± 0.041	4.437ThrLeu: 4.437 ± 0.055	1.411ThrMet: 1.411 ± 0.028	2.07ThrAsn: 2.07 ± 0.036	1.929ThrPro: 1.929 ± 0.038	1.746ThrGln: 1.746 ± 0.033	1.831ThrArg: 1.831 ± 0.034	2.642ThrSer: 2.642 ± 0.046	2.432ThrThr: 2.432 ± 0.044	4.222ThrVal: 4.222 ± 0.058	0.51ThrTrp: 0.51 ± 0.017	2.125ThrTyr: 2.125 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	4.031ValAla: 4.031 ± 0.059	1.335ValCys: 1.335 ± 0.028	3.375ValAsp: 3.375 ± 0.052	4.065ValGlu: 4.065 ± 0.055	3.033ValPhe: 3.033 ± 0.045	3.384ValGly: 3.384 ± 0.054	1.191ValHis: 1.191 ± 0.025	4.762ValIle: 4.762 ± 0.053	4.297ValLys: 4.297 ± 0.055	6.206ValLeu: 6.206 ± 0.067	1.995ValMet: 1.995 ± 0.033	2.78ValAsn: 2.78 ± 0.033	2.272ValPro: 2.272 ± 0.037	2.095ValGln: 2.095 ± 0.036	3.525ValArg: 3.525 ± 0.041	4.525ValSer: 4.525 ± 0.052	3.697ValThr: 3.697 ± 0.063	4.013ValVal: 4.013 ± 0.059	0.688ValTrp: 0.688 ± 0.019	2.758ValTyr: 2.758 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.546TrpAla: 0.546 ± 0.018	0.171TrpCys: 0.171 ± 0.011	0.596TrpAsp: 0.596 ± 0.022	0.751TrpGlu: 0.751 ± 0.02	0.426TrpPhe: 0.426 ± 0.015	0.621TrpGly: 0.621 ± 0.017	0.208TrpHis: 0.208 ± 0.012	0.703TrpIle: 0.703 ± 0.023	0.848TrpLys: 0.848 ± 0.025	0.945TrpLeu: 0.945 ± 0.026	0.368TrpMet: 0.368 ± 0.013	0.601TrpAsn: 0.601 ± 0.019	0.183TrpPro: 0.183 ± 0.01	0.455TrpGln: 0.455 ± 0.017	0.391TrpArg: 0.391 ± 0.015	0.536TrpSer: 0.536 ± 0.019	0.484TrpThr: 0.484 ± 0.017	0.508TrpVal: 0.508 ± 0.016	0.113TrpTrp: 0.113 ± 0.008	0.443TrpTyr: 0.443 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.075TyrAla: 3.075 ± 0.041	0.767TyrCys: 0.767 ± 0.021	2.665TyrAsp: 2.665 ± 0.044	3.394TyrGlu: 3.394 ± 0.05	1.998TyrPhe: 1.998 ± 0.033	3.077TyrGly: 3.077 ± 0.041	1.045TyrHis: 1.045 ± 0.022	2.906TyrIle: 2.906 ± 0.045	2.588TyrLys: 2.588 ± 0.04	4.045TyrLeu: 4.045 ± 0.055	1.248TyrMet: 1.248 ± 0.025	1.876TyrAsn: 1.876 ± 0.036	1.428TyrPro: 1.428 ± 0.032	1.695TyrGln: 1.695 ± 0.032	2.525TyrArg: 2.525 ± 0.043	2.331TyrSer: 2.331 ± 0.039	2.372TyrThr: 2.372 ± 0.04	2.698TyrVal: 2.698 ± 0.039	0.435TyrTrp: 0.435 ± 0.017	2.067TyrTyr: 2.067 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6154 proteins (1750650 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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