Geobacillus virus E3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3UZA9|A0A0H3UZA9_9CAUD Uncharacterized protein OS=Geobacillus virus E3 OX=1572712 GN=E3_011 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84WNGFNFNDD10 pKa = 4.32EE11 pKa = 4.3NQCNCPEE18 pKa = 4.14CRR20 pKa = 11.84GVTDD24 pKa = 3.9INHH27 pKa = 5.86MEE29 pKa = 3.86IDD31 pKa = 4.22VYY33 pKa = 10.97IDD35 pKa = 4.12DD36 pKa = 5.33PNDD39 pKa = 3.67IINDD43 pKa = 3.32YY44 pKa = 11.26FEE46 pKa = 4.39IVKK49 pKa = 10.02NAQSEE54 pKa = 4.55EE55 pKa = 4.04EE56 pKa = 4.55LYY58 pKa = 10.91DD59 pKa = 3.7ILVEE63 pKa = 4.57FYY65 pKa = 10.92NAAVDD70 pKa = 3.68NTYY73 pKa = 10.83KK74 pKa = 10.45QVLLDD79 pKa = 4.48EE80 pKa = 5.05INEE83 pKa = 4.24KK84 pKa = 10.79VSMLNNVQYY93 pKa = 11.36GDD95 pKa = 4.16DD96 pKa = 3.82EE97 pKa = 4.42EE98 pKa = 4.49

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3UZ25|A0A0H3UZ25_9CAUD Uncharacterized protein OS=Geobacillus virus E3 OX=1572712 GN=E3_0192 PE=4 SV=1
MM1 pKa = 7.02VYY3 pKa = 9.62KK4 pKa = 10.16WKK6 pKa = 10.96NSFHH10 pKa = 6.69KK11 pKa = 10.42ASKK14 pKa = 9.08NAEE17 pKa = 4.34HH18 pKa = 6.68FRR20 pKa = 11.84MALSYY25 pKa = 9.99TLEE28 pKa = 4.02NMKK31 pKa = 10.31EE32 pKa = 4.05FYY34 pKa = 10.85NIGWYY39 pKa = 10.14CRR41 pKa = 11.84MVKK44 pKa = 10.09RR45 pKa = 11.84YY46 pKa = 7.28QLKK49 pKa = 10.2RR50 pKa = 11.84SRR52 pKa = 11.84KK53 pKa = 9.19NLFNCC58 pKa = 5.02

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

40012

48

1913

199.1

23.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.514 ± 0.245

1.005 ± 0.096

6.228 ± 0.166

8.837 ± 0.228

4.131 ± 0.14

4.824 ± 0.173

1.729 ± 0.083

8.33 ± 0.197

10.672 ± 0.221

8.742 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.085

6.606 ± 0.158

2.437 ± 0.09

3.619 ± 0.202

3.864 ± 0.101

4.934 ± 0.149

4.831 ± 0.168

5.691 ± 0.172

1.165 ± 0.076

5.308 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski