Amino acid dipepetide frequency for Geobacillus virus E3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.974AlaAla: 1.974 ± 0.349
0.35AlaCys: 0.35 ± 0.087
2.699AlaAsp: 2.699 ± 0.248
3.699AlaGlu: 3.699 ± 0.368
1.999AlaPhe: 1.999 ± 0.215
1.974AlaGly: 1.974 ± 0.32
0.3AlaHis: 0.3 ± 0.064
4.449AlaIle: 4.449 ± 0.419
5.074AlaLys: 5.074 ± 0.407
4.299AlaLeu: 4.299 ± 0.389
1.275AlaMet: 1.275 ± 0.224
3.174AlaAsn: 3.174 ± 0.339
0.85AlaPro: 0.85 ± 0.16
1.35AlaGln: 1.35 ± 0.373
1.375AlaArg: 1.375 ± 0.225
2.124AlaSer: 2.124 ± 0.254
2.649AlaThr: 2.649 ± 0.307
2.699AlaVal: 2.699 ± 0.313
0.675AlaTrp: 0.675 ± 0.124
2.149AlaTyr: 2.149 ± 0.264
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.107
0.1CysCys: 0.1 ± 0.051
0.6CysAsp: 0.6 ± 0.121
0.825CysGlu: 0.825 ± 0.145
0.4CysPhe: 0.4 ± 0.094
0.875CysGly: 0.875 ± 0.183
0.2CysHis: 0.2 ± 0.066
0.65CysIle: 0.65 ± 0.127
0.85CysLys: 0.85 ± 0.14
1.25CysLeu: 1.25 ± 0.152
0.175CysMet: 0.175 ± 0.074
0.6CysAsn: 0.6 ± 0.137
0.475CysPro: 0.475 ± 0.157
0.15CysGln: 0.15 ± 0.06
0.425CysArg: 0.425 ± 0.097
0.7CysSer: 0.7 ± 0.118
0.325CysThr: 0.325 ± 0.082
0.425CysVal: 0.425 ± 0.096
0.075CysTrp: 0.075 ± 0.045
0.5CysTyr: 0.5 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
2.774AspAla: 2.774 ± 0.259
0.55AspCys: 0.55 ± 0.118
4.274AspAsp: 4.274 ± 0.367
6.123AspGlu: 6.123 ± 0.382
3.199AspPhe: 3.199 ± 0.314
3.549AspGly: 3.549 ± 0.371
1.075AspHis: 1.075 ± 0.159
5.174AspIle: 5.174 ± 0.478
7.373AspLys: 7.373 ± 0.48
4.999AspLeu: 4.999 ± 0.345
1.575AspMet: 1.575 ± 0.211
3.474AspAsn: 3.474 ± 0.263
1.25AspPro: 1.25 ± 0.182
1.65AspGln: 1.65 ± 0.192
2.024AspArg: 2.024 ± 0.262
2.524AspSer: 2.524 ± 0.303
2.574AspThr: 2.574 ± 0.252
4.049AspVal: 4.049 ± 0.401
0.625AspTrp: 0.625 ± 0.115
3.449AspTyr: 3.449 ± 0.369
0.0AspXaa: 0.0 ± 0.0
Glu
3.749GluAla: 3.749 ± 0.431
0.85GluCys: 0.85 ± 0.162
4.574GluAsp: 4.574 ± 0.328
7.498GluGlu: 7.498 ± 0.531
4.024GluPhe: 4.024 ± 0.341
3.124GluGly: 3.124 ± 0.288
1.25GluHis: 1.25 ± 0.164
8.673GluIle: 8.673 ± 0.517
9.147GluLys: 9.147 ± 0.517
9.922GluLeu: 9.922 ± 0.644
2.799GluMet: 2.799 ± 0.323
5.174GluAsn: 5.174 ± 0.275
1.55GluPro: 1.55 ± 0.204
4.074GluGln: 4.074 ± 0.364
3.674GluArg: 3.674 ± 0.348
3.324GluSer: 3.324 ± 0.297
3.774GluThr: 3.774 ± 0.324
5.299GluVal: 5.299 ± 0.357
1.25GluTrp: 1.25 ± 0.187
4.649GluTyr: 4.649 ± 0.376
0.0GluXaa: 0.0 ± 0.0
Phe
1.375PheAla: 1.375 ± 0.158
0.575PheCys: 0.575 ± 0.136
3.074PheAsp: 3.074 ± 0.303
3.424PheGlu: 3.424 ± 0.248
1.5PhePhe: 1.5 ± 0.243
2.274PheGly: 2.274 ± 0.29
0.85PheHis: 0.85 ± 0.167
3.149PheIle: 3.149 ± 0.266
3.824PheLys: 3.824 ± 0.329
3.499PheLeu: 3.499 ± 0.295
1.025PheMet: 1.025 ± 0.138
2.949PheAsn: 2.949 ± 0.257
1.4PhePro: 1.4 ± 0.183
0.875PheGln: 0.875 ± 0.16
1.6PheArg: 1.6 ± 0.231
2.499PheSer: 2.499 ± 0.212
1.949PheThr: 1.949 ± 0.243
2.824PheVal: 2.824 ± 0.273
0.35PheTrp: 0.35 ± 0.096
2.299PheTyr: 2.299 ± 0.276
0.0PheXaa: 0.0 ± 0.0
Gly
1.575GlyAla: 1.575 ± 0.224
0.45GlyCys: 0.45 ± 0.109
3.224GlyAsp: 3.224 ± 0.296
3.824GlyGlu: 3.824 ± 0.36
2.299GlyPhe: 2.299 ± 0.221
2.649GlyGly: 2.649 ± 0.292
0.9GlyHis: 0.9 ± 0.141
4.224GlyIle: 4.224 ± 0.343
5.548GlyLys: 5.548 ± 0.396
4.174GlyLeu: 4.174 ± 0.296
1.575GlyMet: 1.575 ± 0.205
2.699GlyAsn: 2.699 ± 0.271
0.025GlyPro: 0.025 ± 0.025
1.45GlyGln: 1.45 ± 0.21
1.999GlyArg: 1.999 ± 0.232
2.549GlySer: 2.549 ± 0.279
2.649GlyThr: 2.649 ± 0.33
3.174GlyVal: 3.174 ± 0.336
0.55GlyTrp: 0.55 ± 0.129
2.699GlyTyr: 2.699 ± 0.239
0.0GlyXaa: 0.0 ± 0.0
His
0.6HisAla: 0.6 ± 0.118
0.325HisCys: 0.325 ± 0.101
1.15HisAsp: 1.15 ± 0.161
1.425HisGlu: 1.425 ± 0.189
0.725HisPhe: 0.725 ± 0.157
0.775HisGly: 0.775 ± 0.125
0.325HisHis: 0.325 ± 0.092
1.45HisIle: 1.45 ± 0.213
1.849HisLys: 1.849 ± 0.223
1.525HisLeu: 1.525 ± 0.19
0.425HisMet: 0.425 ± 0.107
1.275HisAsn: 1.275 ± 0.191
0.45HisPro: 0.45 ± 0.111
0.35HisGln: 0.35 ± 0.109
0.625HisArg: 0.625 ± 0.121
0.875HisSer: 0.875 ± 0.175
0.775HisThr: 0.775 ± 0.15
1.125HisVal: 1.125 ± 0.18
0.2HisTrp: 0.2 ± 0.066
1.05HisTyr: 1.05 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
3.799IleAla: 3.799 ± 0.337
0.95IleCys: 0.95 ± 0.149
6.948IleAsp: 6.948 ± 0.446
8.723IleGlu: 8.723 ± 0.433
2.149IlePhe: 2.149 ± 0.228
3.349IleGly: 3.349 ± 0.375
1.525IleHis: 1.525 ± 0.235
5.773IleIle: 5.773 ± 0.479
10.022IleLys: 10.022 ± 0.463
6.598IleLeu: 6.598 ± 0.436
1.45IleMet: 1.45 ± 0.174
6.348IleAsn: 6.348 ± 0.432
2.824IlePro: 2.824 ± 0.264
2.974IleGln: 2.974 ± 0.293
3.274IleArg: 3.274 ± 0.276
3.549IleSer: 3.549 ± 0.377
3.749IleThr: 3.749 ± 0.313
4.724IleVal: 4.724 ± 0.435
0.65IleTrp: 0.65 ± 0.119
3.899IleTyr: 3.899 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
4.749LysAla: 4.749 ± 0.36
1.0LysCys: 1.0 ± 0.218
6.098LysAsp: 6.098 ± 0.431
11.097LysGlu: 11.097 ± 0.612
4.174LysPhe: 4.174 ± 0.33
4.624LysGly: 4.624 ± 0.436
2.024LysHis: 2.024 ± 0.224
8.498LysIle: 8.498 ± 0.385
9.897LysLys: 9.897 ± 0.617
9.597LysLeu: 9.597 ± 0.488
3.349LysMet: 3.349 ± 0.3
6.448LysAsn: 6.448 ± 0.556
2.699LysPro: 2.699 ± 0.337
5.199LysGln: 5.199 ± 0.477
4.299LysArg: 4.299 ± 0.378
5.548LysSer: 5.548 ± 0.38
4.399LysThr: 4.399 ± 0.286
5.548LysVal: 5.548 ± 0.373
1.275LysTrp: 1.275 ± 0.187
6.198LysTyr: 6.198 ± 0.44
0.0LysXaa: 0.0 ± 0.0
Leu
4.374LeuAla: 4.374 ± 0.58
0.825LeuCys: 0.825 ± 0.17
4.924LeuAsp: 4.924 ± 0.333
6.598LeuGlu: 6.598 ± 0.401
3.049LeuPhe: 3.049 ± 0.282
3.724LeuGly: 3.724 ± 0.395
1.6LeuHis: 1.6 ± 0.182
7.548LeuIle: 7.548 ± 0.406
10.622LeuLys: 10.622 ± 0.476
7.323LeuLeu: 7.323 ± 0.454
2.274LeuMet: 2.274 ± 0.269
6.773LeuAsn: 6.773 ± 0.394
2.224LeuPro: 2.224 ± 0.237
3.124LeuGln: 3.124 ± 0.388
3.699LeuArg: 3.699 ± 0.338
4.874LeuSer: 4.874 ± 0.365
4.624LeuThr: 4.624 ± 0.338
4.074LeuVal: 4.074 ± 0.436
0.975LeuTrp: 0.975 ± 0.145
4.199LeuTyr: 4.199 ± 0.299
0.0LeuXaa: 0.0 ± 0.0
Met
1.125MetAla: 1.125 ± 0.157
0.2MetCys: 0.2 ± 0.071
1.425MetAsp: 1.425 ± 0.185
1.575MetGlu: 1.575 ± 0.204
1.1MetPhe: 1.1 ± 0.184
1.375MetGly: 1.375 ± 0.225
0.375MetHis: 0.375 ± 0.099
1.924MetIle: 1.924 ± 0.213
3.249MetLys: 3.249 ± 0.326
1.824MetLeu: 1.824 ± 0.19
0.625MetMet: 0.625 ± 0.109
2.224MetAsn: 2.224 ± 0.241
0.9MetPro: 0.9 ± 0.146
1.1MetGln: 1.1 ± 0.171
1.25MetArg: 1.25 ± 0.17
1.35MetSer: 1.35 ± 0.22
1.6MetThr: 1.6 ± 0.179
0.925MetVal: 0.925 ± 0.15
0.325MetTrp: 0.325 ± 0.087
0.875MetTyr: 0.875 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
2.774AsnAla: 2.774 ± 0.265
0.775AsnCys: 0.775 ± 0.147
3.599AsnAsp: 3.599 ± 0.357
7.073AsnGlu: 7.073 ± 0.512
2.674AsnPhe: 2.674 ± 0.272
3.999AsnGly: 3.999 ± 0.288
1.075AsnHis: 1.075 ± 0.162
5.099AsnIle: 5.099 ± 0.396
7.848AsnLys: 7.848 ± 0.468
4.949AsnLeu: 4.949 ± 0.35
1.475AsnMet: 1.475 ± 0.207
4.299AsnAsn: 4.299 ± 0.362
2.074AsnPro: 2.074 ± 0.217
2.424AsnGln: 2.424 ± 0.317
2.174AsnArg: 2.174 ± 0.223
3.349AsnSer: 3.349 ± 0.276
3.149AsnThr: 3.149 ± 0.306
3.374AsnVal: 3.374 ± 0.308
0.625AsnTrp: 0.625 ± 0.127
3.249AsnTyr: 3.249 ± 0.273
0.0AsnXaa: 0.0 ± 0.0
Pro
0.8ProAla: 0.8 ± 0.18
0.375ProCys: 0.375 ± 0.096
1.775ProAsp: 1.775 ± 0.223
1.974ProGlu: 1.974 ± 0.23
1.45ProPhe: 1.45 ± 0.187
0.225ProGly: 0.225 ± 0.074
0.55ProHis: 0.55 ± 0.137
2.199ProIle: 2.199 ± 0.288
2.124ProLys: 2.124 ± 0.264
1.849ProLeu: 1.849 ± 0.213
0.6ProMet: 0.6 ± 0.113
1.974ProAsn: 1.974 ± 0.286
0.725ProPro: 0.725 ± 0.146
0.75ProGln: 0.75 ± 0.13
0.75ProArg: 0.75 ± 0.129
1.65ProSer: 1.65 ± 0.181
1.475ProThr: 1.475 ± 0.232
1.35ProVal: 1.35 ± 0.184
0.1ProTrp: 0.1 ± 0.046
1.675ProTyr: 1.675 ± 0.206
0.0ProXaa: 0.0 ± 0.0
Gln
2.224GlnAla: 2.224 ± 0.373
0.25GlnCys: 0.25 ± 0.078
1.25GlnAsp: 1.25 ± 0.155
3.399GlnGlu: 3.399 ± 0.424
1.375GlnPhe: 1.375 ± 0.17
1.575GlnGly: 1.575 ± 0.218
0.425GlnHis: 0.425 ± 0.091
3.574GlnIle: 3.574 ± 0.398
3.124GlnLys: 3.124 ± 0.406
3.799GlnLeu: 3.799 ± 0.374
0.975GlnMet: 0.975 ± 0.15
2.474GlnAsn: 2.474 ± 0.275
0.55GlnPro: 0.55 ± 0.118
1.625GlnGln: 1.625 ± 0.393
1.75GlnArg: 1.75 ± 0.243
1.675GlnSer: 1.675 ± 0.202
1.65GlnThr: 1.65 ± 0.22
1.75GlnVal: 1.75 ± 0.215
0.45GlnTrp: 0.45 ± 0.104
2.299GlnTyr: 2.299 ± 0.271
0.0GlnXaa: 0.0 ± 0.0
Arg
2.149ArgAla: 2.149 ± 0.207
0.35ArgCys: 0.35 ± 0.094
2.549ArgAsp: 2.549 ± 0.244
3.374ArgGlu: 3.374 ± 0.284
1.7ArgPhe: 1.7 ± 0.233
1.874ArgGly: 1.874 ± 0.242
0.625ArgHis: 0.625 ± 0.15
2.949ArgIle: 2.949 ± 0.311
4.199ArgLys: 4.199 ± 0.479
3.224ArgLeu: 3.224 ± 0.348
0.925ArgMet: 0.925 ± 0.153
2.299ArgAsn: 2.299 ± 0.308
0.875ArgPro: 0.875 ± 0.141
1.625ArgGln: 1.625 ± 0.251
1.575ArgArg: 1.575 ± 0.211
1.849ArgSer: 1.849 ± 0.248
1.7ArgThr: 1.7 ± 0.175
2.074ArgVal: 2.074 ± 0.333
0.7ArgTrp: 0.7 ± 0.13
2.024ArgTyr: 2.024 ± 0.26
0.0ArgXaa: 0.0 ± 0.0
Ser
1.924SerAla: 1.924 ± 0.225
0.375SerCys: 0.375 ± 0.083
2.949SerAsp: 2.949 ± 0.315
3.799SerGlu: 3.799 ± 0.401
2.174SerPhe: 2.174 ± 0.197
2.699SerGly: 2.699 ± 0.324
0.875SerHis: 0.875 ± 0.17
4.274SerIle: 4.274 ± 0.393
5.224SerLys: 5.224 ± 0.365
4.249SerLeu: 4.249 ± 0.447
1.1SerMet: 1.1 ± 0.163
3.249SerAsn: 3.249 ± 0.309
1.625SerPro: 1.625 ± 0.231
1.625SerGln: 1.625 ± 0.23
1.899SerArg: 1.899 ± 0.217
3.199SerSer: 3.199 ± 0.477
2.149SerThr: 2.149 ± 0.23
2.874SerVal: 2.874 ± 0.247
0.6SerTrp: 0.6 ± 0.126
2.474SerTyr: 2.474 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
2.624ThrAla: 2.624 ± 0.368
0.5ThrCys: 0.5 ± 0.143
2.649ThrAsp: 2.649 ± 0.276
3.424ThrGlu: 3.424 ± 0.328
2.024ThrPhe: 2.024 ± 0.217
3.274ThrGly: 3.274 ± 0.242
0.75ThrHis: 0.75 ± 0.15
3.924ThrIle: 3.924 ± 0.323
4.274ThrLys: 4.274 ± 0.287
4.274ThrLeu: 4.274 ± 0.338
1.075ThrMet: 1.075 ± 0.16
2.749ThrAsn: 2.749 ± 0.275
1.425ThrPro: 1.425 ± 0.216
1.675ThrGln: 1.675 ± 0.212
1.5ThrArg: 1.5 ± 0.17
2.349ThrSer: 2.349 ± 0.245
2.624ThrThr: 2.624 ± 0.33
3.699ThrVal: 3.699 ± 0.34
0.575ThrTrp: 0.575 ± 0.126
2.924ThrTyr: 2.924 ± 0.275
0.0ThrXaa: 0.0 ± 0.0
Val
3.149ValAla: 3.149 ± 0.321
0.55ValCys: 0.55 ± 0.103
4.049ValAsp: 4.049 ± 0.329
4.924ValGlu: 4.924 ± 0.273
2.249ValPhe: 2.249 ± 0.228
2.724ValGly: 2.724 ± 0.219
1.125ValHis: 1.125 ± 0.214
4.499ValIle: 4.499 ± 0.34
6.023ValLys: 6.023 ± 0.48
5.149ValLeu: 5.149 ± 0.38
1.375ValMet: 1.375 ± 0.184
3.299ValAsn: 3.299 ± 0.258
1.05ValPro: 1.05 ± 0.159
1.7ValGln: 1.7 ± 0.213
1.824ValArg: 1.824 ± 0.209
2.374ValSer: 2.374 ± 0.258
3.199ValThr: 3.199 ± 0.311
3.874ValVal: 3.874 ± 0.375
0.85ValTrp: 0.85 ± 0.18
2.924ValTyr: 2.924 ± 0.318
0.0ValXaa: 0.0 ± 0.0
Trp
0.525TrpAla: 0.525 ± 0.13
0.1TrpCys: 0.1 ± 0.049
0.675TrpAsp: 0.675 ± 0.126
1.025TrpGlu: 1.025 ± 0.163
0.25TrpPhe: 0.25 ± 0.077
0.425TrpGly: 0.425 ± 0.115
0.275TrpHis: 0.275 ± 0.088
1.25TrpIle: 1.25 ± 0.169
1.125TrpLys: 1.125 ± 0.185
0.9TrpLeu: 0.9 ± 0.147
0.325TrpMet: 0.325 ± 0.099
1.025TrpAsn: 1.025 ± 0.177
0.0TrpPro: 0.0 ± 0.0
0.55TrpGln: 0.55 ± 0.119
0.625TrpArg: 0.625 ± 0.128
0.65TrpSer: 0.65 ± 0.117
0.6TrpThr: 0.6 ± 0.094
0.45TrpVal: 0.45 ± 0.119
0.1TrpTrp: 0.1 ± 0.045
0.775TrpTyr: 0.775 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.424TyrAla: 2.424 ± 0.254
0.6TyrCys: 0.6 ± 0.125
3.799TyrAsp: 3.799 ± 0.345
4.574TyrGlu: 4.574 ± 0.321
2.799TyrPhe: 2.799 ± 0.257
3.149TyrGly: 3.149 ± 0.336
1.175TyrHis: 1.175 ± 0.189
4.024TyrIle: 4.024 ± 0.313
5.324TyrLys: 5.324 ± 0.362
4.024TyrLeu: 4.024 ± 0.326
0.925TyrMet: 0.925 ± 0.179
3.549TyrAsn: 3.549 ± 0.296
1.4TyrPro: 1.4 ± 0.26
1.924TyrGln: 1.924 ± 0.259
2.299TyrArg: 2.299 ± 0.244
2.324TyrSer: 2.324 ± 0.231
2.699TyrThr: 2.699 ± 0.259
2.599TyrVal: 2.599 ± 0.268
0.7TyrTrp: 0.7 ± 0.126
2.774TyrTyr: 2.774 ± 0.321
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 201 proteins (40012 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski