Hymenobacter sp. APR13

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; unclassified Hymenobacter

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076HY11|A0A076HY11_9BACT Uncharacterized protein OS=Hymenobacter sp. APR13 OX=1356852 GN=N008_16230 PE=4 SV=1
MM1 pKa = 7.45NGFTINCDD9 pKa = 2.48SWYY12 pKa = 10.81VDD14 pKa = 3.78LFAFDD19 pKa = 4.41RR20 pKa = 11.84DD21 pKa = 3.48EE22 pKa = 4.72GLNDD26 pKa = 3.97TDD28 pKa = 3.6WLADD32 pKa = 3.72DD33 pKa = 5.25SYY35 pKa = 11.12PAAVPLPITGLEE47 pKa = 4.53DD48 pKa = 2.94IQAIYY53 pKa = 10.49EE54 pKa = 4.12NYY56 pKa = 9.17AEE58 pKa = 4.26KK59 pKa = 10.49WEE61 pKa = 4.39DD62 pKa = 3.4AAGEE66 pKa = 4.09EE67 pKa = 4.56AAHH70 pKa = 6.84DD71 pKa = 3.96CAALILLRR79 pKa = 11.84VQEE82 pKa = 4.74LFNAAKK88 pKa = 10.14GVAAQQLKK96 pKa = 8.91WATLPIYY103 pKa = 8.53VTSHH107 pKa = 6.68DD108 pKa = 4.07AYY110 pKa = 10.22IEE112 pKa = 3.94LLYY115 pKa = 10.68RR116 pKa = 11.84AA117 pKa = 4.72

Molecular weight: 13.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076HUB9|A0A076HUB9_9BACT Uncharacterized protein OS=Hymenobacter sp. APR13 OX=1356852 GN=N008_14530 PE=4 SV=1
MM1 pKa = 7.37GVGGPQHH8 pKa = 5.45ITLRR12 pKa = 11.84QQSRR16 pKa = 11.84QRR18 pKa = 11.84GGGGAVAQLVPTHH31 pKa = 6.23HH32 pKa = 6.74QMAQPRR38 pKa = 11.84RR39 pKa = 11.84QPQPRR44 pKa = 11.84QRR46 pKa = 11.84PAVRR50 pKa = 11.84RR51 pKa = 11.84GFQQ54 pKa = 2.8

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4229

0

4229

1377571

29

2608

325.7

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.821 ± 0.053

0.694 ± 0.011

4.897 ± 0.029

5.25 ± 0.039

4.025 ± 0.023

7.639 ± 0.042

2.063 ± 0.021

4.119 ± 0.041

3.733 ± 0.047

11.277 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.882 ± 0.021

3.579 ± 0.037

5.341 ± 0.033

4.859 ± 0.035

6.368 ± 0.041

5.352 ± 0.03

6.299 ± 0.052

7.154 ± 0.032

1.231 ± 0.017

3.418 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski