Amino acid dipepetide frequency for Hymenobacter sp. APR13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.835AlaAla: 14.835 ± 0.163
0.802AlaCys: 0.802 ± 0.026
5.884AlaAsp: 5.884 ± 0.07
5.91AlaGlu: 5.91 ± 0.07
3.697AlaPhe: 3.697 ± 0.051
9.419AlaGly: 9.419 ± 0.098
1.996AlaHis: 1.996 ± 0.037
3.807AlaIle: 3.807 ± 0.056
3.381AlaLys: 3.381 ± 0.066
11.506AlaLeu: 11.506 ± 0.107
1.848AlaMet: 1.848 ± 0.037
3.206AlaAsn: 3.206 ± 0.063
5.69AlaPro: 5.69 ± 0.096
5.034AlaGln: 5.034 ± 0.062
6.544AlaArg: 6.544 ± 0.085
5.458AlaSer: 5.458 ± 0.07
7.3AlaThr: 7.3 ± 0.114
7.454AlaVal: 7.454 ± 0.082
1.264AlaTrp: 1.264 ± 0.034
3.177AlaTyr: 3.177 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.624CysAla: 0.624 ± 0.024
0.102CysCys: 0.102 ± 0.01
0.319CysAsp: 0.319 ± 0.015
0.321CysGlu: 0.321 ± 0.018
0.287CysPhe: 0.287 ± 0.015
0.632CysGly: 0.632 ± 0.022
0.219CysHis: 0.219 ± 0.015
0.308CysIle: 0.308 ± 0.016
0.179CysLys: 0.179 ± 0.011
0.74CysLeu: 0.74 ± 0.026
0.112CysMet: 0.112 ± 0.009
0.25CysAsn: 0.25 ± 0.014
0.421CysPro: 0.421 ± 0.023
0.335CysGln: 0.335 ± 0.016
0.437CysArg: 0.437 ± 0.018
0.409CysSer: 0.409 ± 0.019
0.421CysThr: 0.421 ± 0.02
0.474CysVal: 0.474 ± 0.018
0.09CysTrp: 0.09 ± 0.008
0.263CysTyr: 0.263 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
5.189AspAla: 5.189 ± 0.072
0.33AspCys: 0.33 ± 0.017
2.33AspAsp: 2.33 ± 0.044
2.981AspGlu: 2.981 ± 0.048
2.481AspPhe: 2.481 ± 0.039
3.826AspGly: 3.826 ± 0.075
0.825AspHis: 0.825 ± 0.025
2.25AspIle: 2.25 ± 0.04
2.229AspLys: 2.229 ± 0.05
5.039AspLeu: 5.039 ± 0.077
0.948AspMet: 0.948 ± 0.027
1.853AspAsn: 1.853 ± 0.042
2.348AspPro: 2.348 ± 0.039
1.962AspGln: 1.962 ± 0.035
2.4AspArg: 2.4 ± 0.043
2.631AspSer: 2.631 ± 0.053
2.764AspThr: 2.764 ± 0.052
3.664AspVal: 3.664 ± 0.048
0.736AspTrp: 0.736 ± 0.025
2.178AspTyr: 2.178 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
5.97GluAla: 5.97 ± 0.082
0.276GluCys: 0.276 ± 0.016
2.192GluAsp: 2.192 ± 0.044
2.952GluGlu: 2.952 ± 0.051
2.132GluPhe: 2.132 ± 0.037
3.34GluGly: 3.34 ± 0.041
1.117GluHis: 1.117 ± 0.033
2.45GluIle: 2.45 ± 0.038
2.643GluLys: 2.643 ± 0.059
6.395GluLeu: 6.395 ± 0.084
1.259GluMet: 1.259 ± 0.032
1.854GluAsn: 1.854 ± 0.043
2.329GluPro: 2.329 ± 0.05
3.087GluGln: 3.087 ± 0.056
3.15GluArg: 3.15 ± 0.057
2.171GluSer: 2.171 ± 0.042
2.879GluThr: 2.879 ± 0.05
3.929GluVal: 3.929 ± 0.063
0.641GluTrp: 0.641 ± 0.021
1.731GluTyr: 1.731 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.661PheAla: 3.661 ± 0.051
0.335PheCys: 0.335 ± 0.018
2.349PheAsp: 2.349 ± 0.044
2.21PheGlu: 2.21 ± 0.046
1.787PhePhe: 1.787 ± 0.037
3.365PheGly: 3.365 ± 0.051
0.722PheHis: 0.722 ± 0.024
1.636PheIle: 1.636 ± 0.041
1.292PheLys: 1.292 ± 0.034
3.928PheLeu: 3.928 ± 0.059
0.74PheMet: 0.74 ± 0.027
1.635PheAsn: 1.635 ± 0.04
1.767PhePro: 1.767 ± 0.032
1.625PheGln: 1.625 ± 0.037
2.795PheArg: 2.795 ± 0.037
2.679PheSer: 2.679 ± 0.047
2.685PheThr: 2.685 ± 0.052
2.913PheVal: 2.913 ± 0.046
0.569PheTrp: 0.569 ± 0.021
1.56PheTyr: 1.56 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
6.913GlyAla: 6.913 ± 0.089
0.661GlyCys: 0.661 ± 0.026
2.939GlyAsp: 2.939 ± 0.065
3.529GlyGlu: 3.529 ± 0.057
3.389GlyPhe: 3.389 ± 0.061
6.072GlyGly: 6.072 ± 0.096
1.582GlyHis: 1.582 ± 0.034
3.734GlyIle: 3.734 ± 0.062
3.28GlyLys: 3.28 ± 0.063
8.577GlyLeu: 8.577 ± 0.093
1.561GlyMet: 1.561 ± 0.04
2.668GlyAsn: 2.668 ± 0.061
3.117GlyPro: 3.117 ± 0.058
4.029GlyGln: 4.029 ± 0.065
5.135GlyArg: 5.135 ± 0.071
4.596GlySer: 4.596 ± 0.069
5.429GlyThr: 5.429 ± 0.112
5.184GlyVal: 5.184 ± 0.058
1.1GlyTrp: 1.1 ± 0.03
2.895GlyTyr: 2.895 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
1.834HisAla: 1.834 ± 0.044
0.192HisCys: 0.192 ± 0.012
1.125HisAsp: 1.125 ± 0.029
1.083HisGlu: 1.083 ± 0.026
1.044HisPhe: 1.044 ± 0.032
1.55HisGly: 1.55 ± 0.042
0.621HisHis: 0.621 ± 0.026
0.931HisIle: 0.931 ± 0.029
0.612HisLys: 0.612 ± 0.02
2.4HisLeu: 2.4 ± 0.044
0.36HisMet: 0.36 ± 0.016
0.722HisAsn: 0.722 ± 0.023
1.312HisPro: 1.312 ± 0.03
0.968HisGln: 0.968 ± 0.026
1.305HisArg: 1.305 ± 0.035
0.979HisSer: 0.979 ± 0.028
1.229HisThr: 1.229 ± 0.035
1.206HisVal: 1.206 ± 0.033
0.295HisTrp: 0.295 ± 0.016
0.863HisTyr: 0.863 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
3.652IleAla: 3.652 ± 0.055
0.378IleCys: 0.378 ± 0.018
2.306IleAsp: 2.306 ± 0.05
2.411IleGlu: 2.411 ± 0.049
1.565IlePhe: 1.565 ± 0.035
3.547IleGly: 3.547 ± 0.059
0.722IleHis: 0.722 ± 0.024
2.136IleIle: 2.136 ± 0.054
1.669IleLys: 1.669 ± 0.046
3.673IleLeu: 3.673 ± 0.06
0.749IleMet: 0.749 ± 0.026
1.731IleAsn: 1.731 ± 0.041
2.017IlePro: 2.017 ± 0.042
1.567IleGln: 1.567 ± 0.037
2.889IleArg: 2.889 ± 0.045
2.777IleSer: 2.777 ± 0.043
2.787IleThr: 2.787 ± 0.052
2.888IleVal: 2.888 ± 0.056
0.435IleTrp: 0.435 ± 0.018
1.29IleTyr: 1.29 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.885LysAla: 3.885 ± 0.073
0.176LysCys: 0.176 ± 0.012
1.907LysAsp: 1.907 ± 0.045
2.106LysGlu: 2.106 ± 0.049
1.244LysPhe: 1.244 ± 0.032
2.623LysGly: 2.623 ± 0.051
0.769LysHis: 0.769 ± 0.025
1.681LysIle: 1.681 ± 0.037
2.148LysLys: 2.148 ± 0.055
3.984LysLeu: 3.984 ± 0.066
1.034LysMet: 1.034 ± 0.033
1.478LysAsn: 1.478 ± 0.038
2.154LysPro: 2.154 ± 0.045
1.696LysGln: 1.696 ± 0.043
1.979LysArg: 1.979 ± 0.041
1.845LysSer: 1.845 ± 0.04
2.321LysThr: 2.321 ± 0.046
2.673LysVal: 2.673 ± 0.054
0.386LysTrp: 0.386 ± 0.017
1.246LysTyr: 1.246 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
12.384LeuAla: 12.384 ± 0.115
0.771LeuCys: 0.771 ± 0.021
5.629LeuAsp: 5.629 ± 0.084
5.273LeuGlu: 5.273 ± 0.071
3.958LeuPhe: 3.958 ± 0.068
8.016LeuGly: 8.016 ± 0.09
2.502LeuHis: 2.502 ± 0.048
3.956LeuIle: 3.956 ± 0.064
3.834LeuLys: 3.834 ± 0.064
14.645LeuLeu: 14.645 ± 0.191
2.022LeuMet: 2.022 ± 0.042
4.075LeuAsn: 4.075 ± 0.052
6.914LeuPro: 6.914 ± 0.092
4.929LeuGln: 4.929 ± 0.072
8.22LeuArg: 8.22 ± 0.087
6.424LeuSer: 6.424 ± 0.076
7.42LeuThr: 7.42 ± 0.086
7.425LeuVal: 7.425 ± 0.089
1.164LeuTrp: 1.164 ± 0.034
3.209LeuTyr: 3.209 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.031MetAla: 2.031 ± 0.04
0.101MetCys: 0.101 ± 0.008
0.795MetAsp: 0.795 ± 0.024
0.933MetGlu: 0.933 ± 0.028
0.571MetPhe: 0.571 ± 0.023
1.344MetGly: 1.344 ± 0.031
0.412MetHis: 0.412 ± 0.019
0.69MetIle: 0.69 ± 0.025
1.038MetLys: 1.038 ± 0.031
2.271MetLeu: 2.271 ± 0.045
0.473MetMet: 0.473 ± 0.019
0.729MetAsn: 0.729 ± 0.022
1.285MetPro: 1.285 ± 0.038
0.961MetGln: 0.961 ± 0.029
1.266MetArg: 1.266 ± 0.03
1.108MetSer: 1.108 ± 0.03
1.041MetThr: 1.041 ± 0.029
1.19MetVal: 1.19 ± 0.032
0.154MetTrp: 0.154 ± 0.01
0.425MetTyr: 0.425 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.27AsnAla: 3.27 ± 0.057
0.274AsnCys: 0.274 ± 0.014
1.726AsnAsp: 1.726 ± 0.044
1.67AsnGlu: 1.67 ± 0.035
1.536AsnPhe: 1.536 ± 0.036
3.069AsnGly: 3.069 ± 0.054
0.622AsnHis: 0.622 ± 0.023
1.644AsnIle: 1.644 ± 0.037
1.259AsnLys: 1.259 ± 0.036
3.762AsnLeu: 3.762 ± 0.057
0.645AsnMet: 0.645 ± 0.023
1.529AsnAsn: 1.529 ± 0.059
2.473AsnPro: 2.473 ± 0.05
1.611AsnGln: 1.611 ± 0.039
2.117AsnArg: 2.117 ± 0.042
2.019AsnSer: 2.019 ± 0.049
2.239AsnThr: 2.239 ± 0.052
2.491AsnVal: 2.491 ± 0.051
0.483AsnTrp: 0.483 ± 0.018
1.348AsnTyr: 1.348 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
8.103ProAla: 8.103 ± 0.123
0.272ProCys: 0.272 ± 0.015
3.484ProAsp: 3.484 ± 0.052
3.645ProGlu: 3.645 ± 0.06
1.886ProPhe: 1.886 ± 0.04
4.328ProGly: 4.328 ± 0.061
1.032ProHis: 1.032 ± 0.03
1.905ProIle: 1.905 ± 0.036
1.626ProLys: 1.626 ± 0.039
5.326ProLeu: 5.326 ± 0.073
0.822ProMet: 0.822 ± 0.026
1.928ProAsn: 1.928 ± 0.041
2.143ProPro: 2.143 ± 0.052
2.173ProGln: 2.173 ± 0.044
2.605ProArg: 2.605 ± 0.045
2.287ProSer: 2.287 ± 0.041
3.415ProThr: 3.415 ± 0.052
4.284ProVal: 4.284 ± 0.059
0.573ProTrp: 0.573 ± 0.021
1.572ProTyr: 1.572 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
5.243GlnAla: 5.243 ± 0.074
0.21GlnCys: 0.21 ± 0.012
2.097GlnAsp: 2.097 ± 0.038
2.56GlnGlu: 2.56 ± 0.051
1.809GlnPhe: 1.809 ± 0.037
2.95GlnGly: 2.95 ± 0.056
1.273GlnHis: 1.273 ± 0.035
1.798GlnIle: 1.798 ± 0.041
1.755GlnLys: 1.755 ± 0.044
6.274GlnLeu: 6.274 ± 0.092
0.945GlnMet: 0.945 ± 0.028
1.676GlnAsn: 1.676 ± 0.035
3.21GlnPro: 3.21 ± 0.057
3.586GlnGln: 3.586 ± 0.07
3.197GlnArg: 3.197 ± 0.05
1.929GlnSer: 1.929 ± 0.04
2.6GlnThr: 2.6 ± 0.046
3.417GlnVal: 3.417 ± 0.052
0.552GlnTrp: 0.552 ± 0.023
1.511GlnTyr: 1.511 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
5.658ArgAla: 5.658 ± 0.069
0.36ArgCys: 0.36 ± 0.016
2.629ArgAsp: 2.629 ± 0.045
3.42ArgGlu: 3.42 ± 0.059
2.761ArgPhe: 2.761 ± 0.048
3.719ArgGly: 3.719 ± 0.053
1.649ArgHis: 1.649 ± 0.033
2.912ArgIle: 2.912 ± 0.048
2.282ArgLys: 2.282 ± 0.054
7.562ArgLeu: 7.562 ± 0.083
1.295ArgMet: 1.295 ± 0.035
2.285ArgAsn: 2.285 ± 0.041
3.503ArgPro: 3.503 ± 0.054
4.116ArgGln: 4.116 ± 0.064
4.885ArgArg: 4.885 ± 0.073
2.798ArgSer: 2.798 ± 0.047
3.823ArgThr: 3.823 ± 0.055
4.459ArgVal: 4.459 ± 0.057
0.91ArgTrp: 0.91 ± 0.028
2.658ArgTyr: 2.658 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
5.492SerAla: 5.492 ± 0.07
0.462SerCys: 0.462 ± 0.02
2.354SerAsp: 2.354 ± 0.042
2.446SerGlu: 2.446 ± 0.045
2.647SerPhe: 2.647 ± 0.044
4.78SerGly: 4.78 ± 0.083
0.976SerHis: 0.976 ± 0.027
2.43SerIle: 2.43 ± 0.047
1.923SerLys: 1.923 ± 0.041
5.65SerLeu: 5.65 ± 0.077
1.008SerMet: 1.008 ± 0.029
1.843SerAsn: 1.843 ± 0.051
2.691SerPro: 2.691 ± 0.044
2.128SerGln: 2.128 ± 0.044
3.251SerArg: 3.251 ± 0.052
3.235SerSer: 3.235 ± 0.066
3.456SerThr: 3.456 ± 0.071
3.942SerVal: 3.942 ± 0.059
0.735SerTrp: 0.735 ± 0.021
2.072SerTyr: 2.072 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
6.964ThrAla: 6.964 ± 0.112
0.32ThrCys: 0.32 ± 0.015
3.403ThrAsp: 3.403 ± 0.053
3.133ThrGlu: 3.133 ± 0.047
2.499ThrPhe: 2.499 ± 0.052
5.4ThrGly: 5.4 ± 0.095
1.198ThrHis: 1.198 ± 0.031
2.738ThrIle: 2.738 ± 0.049
1.882ThrLys: 1.882 ± 0.04
6.928ThrLeu: 6.928 ± 0.079
0.934ThrMet: 0.934 ± 0.024
2.12ThrAsn: 2.12 ± 0.052
4.084ThrPro: 4.084 ± 0.059
2.491ThrGln: 2.491 ± 0.044
3.355ThrArg: 3.355 ± 0.05
3.429ThrSer: 3.429 ± 0.069
4.335ThrThr: 4.335 ± 0.094
4.859ThrVal: 4.859 ± 0.074
0.73ThrTrp: 0.73 ± 0.026
2.19ThrTyr: 2.19 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
8.114ValAla: 8.114 ± 0.091
0.536ValCys: 0.536 ± 0.019
3.084ValAsp: 3.084 ± 0.054
3.642ValGlu: 3.642 ± 0.058
2.838ValPhe: 2.838 ± 0.048
5.098ValGly: 5.098 ± 0.072
1.237ValHis: 1.237 ± 0.033
2.62ValIle: 2.62 ± 0.052
2.624ValLys: 2.624 ± 0.048
8.471ValLeu: 8.471 ± 0.085
1.283ValMet: 1.283 ± 0.035
2.382ValAsn: 2.382 ± 0.049
3.837ValPro: 3.837 ± 0.05
3.559ValGln: 3.559 ± 0.053
4.861ValArg: 4.861 ± 0.065
4.183ValSer: 4.183 ± 0.062
4.154ValThr: 4.154 ± 0.074
6.006ValVal: 6.006 ± 0.074
0.828ValTrp: 0.828 ± 0.026
2.181ValTyr: 2.181 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.085TrpAla: 1.085 ± 0.028
0.099TrpCys: 0.099 ± 0.008
0.528TrpAsp: 0.528 ± 0.023
0.581TrpGlu: 0.581 ± 0.022
0.5TrpPhe: 0.5 ± 0.017
0.78TrpGly: 0.78 ± 0.025
0.34TrpHis: 0.34 ± 0.016
0.368TrpIle: 0.368 ± 0.015
0.467TrpLys: 0.467 ± 0.019
1.86TrpLeu: 1.86 ± 0.047
0.262TrpMet: 0.262 ± 0.014
0.473TrpAsn: 0.473 ± 0.02
0.544TrpPro: 0.544 ± 0.025
0.899TrpGln: 0.899 ± 0.032
0.869TrpArg: 0.869 ± 0.028
0.603TrpSer: 0.603 ± 0.02
0.571TrpThr: 0.571 ± 0.021
0.845TrpVal: 0.845 ± 0.03
0.224TrpTrp: 0.224 ± 0.013
0.407TrpTyr: 0.407 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.306TyrAla: 3.306 ± 0.049
0.286TyrCys: 0.286 ± 0.014
1.884TyrAsp: 1.884 ± 0.037
1.693TyrGlu: 1.693 ± 0.033
1.62TyrPhe: 1.62 ± 0.034
2.532TyrGly: 2.532 ± 0.045
0.819TyrHis: 0.819 ± 0.028
1.192TyrIle: 1.192 ± 0.028
1.212TyrLys: 1.212 ± 0.036
3.778TyrLeu: 3.778 ± 0.054
0.518TyrMet: 0.518 ± 0.02
1.351TyrAsn: 1.351 ± 0.035
1.569TyrPro: 1.569 ± 0.038
1.84TyrGln: 1.84 ± 0.035
2.423TyrArg: 2.423 ± 0.047
1.96TyrSer: 1.96 ± 0.038
2.123TyrThr: 2.123 ± 0.04
2.238TyrVal: 2.238 ± 0.04
0.436TyrTrp: 0.436 ± 0.018
1.397TyrTyr: 1.397 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4229 proteins (1377571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski