Amino acid dipepetide frequency for Klebsiella phage vB_KpnM_FZ14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.719AlaAla: 7.719 ± 1.181
0.684AlaCys: 0.684 ± 0.368
5.179AlaAsp: 5.179 ± 0.709
5.277AlaGlu: 5.277 ± 0.851
2.736AlaPhe: 2.736 ± 0.539
5.765AlaGly: 5.765 ± 0.804
1.173AlaHis: 1.173 ± 0.365
4.202AlaIle: 4.202 ± 0.714
5.277AlaLys: 5.277 ± 0.788
6.742AlaLeu: 6.742 ± 0.762
2.247AlaMet: 2.247 ± 0.404
3.127AlaAsn: 3.127 ± 0.518
2.443AlaPro: 2.443 ± 0.475
4.886AlaGln: 4.886 ± 0.81
4.593AlaArg: 4.593 ± 0.803
5.765AlaSer: 5.765 ± 0.901
4.397AlaThr: 4.397 ± 0.702
5.765AlaVal: 5.765 ± 0.815
0.977AlaTrp: 0.977 ± 0.323
2.345AlaTyr: 2.345 ± 0.406
0.0AlaXaa: 0.0 ± 0.0
Cys
1.27CysAla: 1.27 ± 0.374
0.0CysCys: 0.0 ± 0.0
1.075CysAsp: 1.075 ± 0.334
1.173CysGlu: 1.173 ± 0.302
0.098CysPhe: 0.098 ± 0.091
0.684CysGly: 0.684 ± 0.256
0.293CysHis: 0.293 ± 0.171
1.075CysIle: 1.075 ± 0.406
0.879CysLys: 0.879 ± 0.399
0.977CysLeu: 0.977 ± 0.33
0.098CysMet: 0.098 ± 0.091
0.586CysAsn: 0.586 ± 0.248
1.27CysPro: 1.27 ± 0.502
0.391CysGln: 0.391 ± 0.154
0.879CysArg: 0.879 ± 0.28
1.173CysSer: 1.173 ± 0.301
1.075CysThr: 1.075 ± 0.322
0.879CysVal: 0.879 ± 0.406
0.098CysTrp: 0.098 ± 0.103
0.391CysTyr: 0.391 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
5.667AspAla: 5.667 ± 0.827
0.782AspCys: 0.782 ± 0.317
3.322AspAsp: 3.322 ± 0.651
3.518AspGlu: 3.518 ± 0.715
2.638AspPhe: 2.638 ± 0.574
6.742AspGly: 6.742 ± 0.959
0.782AspHis: 0.782 ± 0.274
4.788AspIle: 4.788 ± 0.592
2.638AspLys: 2.638 ± 0.393
4.397AspLeu: 4.397 ± 0.722
2.052AspMet: 2.052 ± 0.486
3.518AspAsn: 3.518 ± 0.573
2.345AspPro: 2.345 ± 0.426
1.759AspGln: 1.759 ± 0.407
2.931AspArg: 2.931 ± 0.71
3.42AspSer: 3.42 ± 0.542
4.006AspThr: 4.006 ± 0.621
5.277AspVal: 5.277 ± 0.726
1.466AspTrp: 1.466 ± 0.343
2.247AspTyr: 2.247 ± 0.439
0.0AspXaa: 0.0 ± 0.0
Glu
3.811GluAla: 3.811 ± 0.676
0.782GluCys: 0.782 ± 0.326
2.931GluAsp: 2.931 ± 0.558
3.518GluGlu: 3.518 ± 0.507
2.247GluPhe: 2.247 ± 0.505
3.225GluGly: 3.225 ± 0.462
1.563GluHis: 1.563 ± 0.36
3.225GluIle: 3.225 ± 0.552
3.713GluLys: 3.713 ± 0.822
4.886GluLeu: 4.886 ± 0.639
1.563GluMet: 1.563 ± 0.57
2.541GluAsn: 2.541 ± 0.58
0.977GluPro: 0.977 ± 0.276
3.811GluGln: 3.811 ± 0.628
3.029GluArg: 3.029 ± 0.619
3.225GluSer: 3.225 ± 0.585
3.029GluThr: 3.029 ± 0.548
3.225GluVal: 3.225 ± 0.492
1.075GluTrp: 1.075 ± 0.391
2.931GluTyr: 2.931 ± 0.45
0.0GluXaa: 0.0 ± 0.0
Phe
2.345PheAla: 2.345 ± 0.576
0.586PheCys: 0.586 ± 0.255
3.225PheAsp: 3.225 ± 0.479
2.443PheGlu: 2.443 ± 0.395
0.977PhePhe: 0.977 ± 0.331
2.834PheGly: 2.834 ± 0.432
0.391PheHis: 0.391 ± 0.203
2.931PheIle: 2.931 ± 0.547
2.052PheLys: 2.052 ± 0.413
1.857PheLeu: 1.857 ± 0.432
0.684PheMet: 0.684 ± 0.224
2.541PheAsn: 2.541 ± 0.421
1.27PhePro: 1.27 ± 0.324
1.27PheGln: 1.27 ± 0.372
2.15PheArg: 2.15 ± 0.487
2.247PheSer: 2.247 ± 0.592
2.736PheThr: 2.736 ± 0.511
2.443PheVal: 2.443 ± 0.528
0.489PheTrp: 0.489 ± 0.184
1.173PheTyr: 1.173 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
4.593GlyAla: 4.593 ± 0.902
1.661GlyCys: 1.661 ± 0.61
5.081GlyAsp: 5.081 ± 0.625
3.811GlyGlu: 3.811 ± 0.505
2.638GlyPhe: 2.638 ± 0.471
6.058GlyGly: 6.058 ± 0.976
1.173GlyHis: 1.173 ± 0.557
4.69GlyIle: 4.69 ± 0.819
4.397GlyLys: 4.397 ± 0.698
5.277GlyLeu: 5.277 ± 0.738
1.954GlyMet: 1.954 ± 0.413
3.127GlyAsn: 3.127 ± 0.606
1.661GlyPro: 1.661 ± 0.418
3.322GlyGln: 3.322 ± 0.412
4.006GlyArg: 4.006 ± 0.562
3.713GlySer: 3.713 ± 0.612
5.374GlyThr: 5.374 ± 1.178
6.058GlyVal: 6.058 ± 0.773
0.977GlyTrp: 0.977 ± 0.31
4.299GlyTyr: 4.299 ± 0.457
0.0GlyXaa: 0.0 ± 0.0
His
1.173HisAla: 1.173 ± 0.387
0.782HisCys: 0.782 ± 0.249
1.27HisAsp: 1.27 ± 0.35
0.684HisGlu: 0.684 ± 0.252
0.879HisPhe: 0.879 ± 0.269
2.638HisGly: 2.638 ± 0.836
1.075HisHis: 1.075 ± 0.373
1.368HisIle: 1.368 ± 0.468
1.075HisLys: 1.075 ± 0.33
1.27HisLeu: 1.27 ± 0.286
0.391HisMet: 0.391 ± 0.2
0.977HisAsn: 0.977 ± 0.299
0.293HisPro: 0.293 ± 0.156
0.293HisGln: 0.293 ± 0.153
1.075HisArg: 1.075 ± 0.364
1.661HisSer: 1.661 ± 0.356
0.586HisThr: 0.586 ± 0.206
1.368HisVal: 1.368 ± 0.351
0.293HisTrp: 0.293 ± 0.164
0.977HisTyr: 0.977 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
4.593IleAla: 4.593 ± 0.711
1.27IleCys: 1.27 ± 0.349
4.104IleAsp: 4.104 ± 0.496
3.811IleGlu: 3.811 ± 0.625
1.466IlePhe: 1.466 ± 0.465
3.322IleGly: 3.322 ± 0.45
1.075IleHis: 1.075 ± 0.34
3.127IleIle: 3.127 ± 0.639
4.593IleLys: 4.593 ± 0.717
2.834IleLeu: 2.834 ± 0.389
2.15IleMet: 2.15 ± 0.391
2.736IleAsn: 2.736 ± 0.649
3.225IlePro: 3.225 ± 0.416
1.759IleGln: 1.759 ± 0.458
3.811IleArg: 3.811 ± 0.637
2.443IleSer: 2.443 ± 0.601
4.104IleThr: 4.104 ± 0.496
4.983IleVal: 4.983 ± 0.594
0.586IleTrp: 0.586 ± 0.249
1.173IleTyr: 1.173 ± 0.32
0.0IleXaa: 0.0 ± 0.0
Lys
5.961LysAla: 5.961 ± 0.955
0.977LysCys: 0.977 ± 0.324
3.811LysAsp: 3.811 ± 0.585
2.443LysGlu: 2.443 ± 0.793
2.931LysPhe: 2.931 ± 0.366
3.615LysGly: 3.615 ± 0.556
1.27LysHis: 1.27 ± 0.338
3.322LysIle: 3.322 ± 0.6
2.541LysLys: 2.541 ± 0.442
5.081LysLeu: 5.081 ± 0.808
1.857LysMet: 1.857 ± 0.42
2.247LysAsn: 2.247 ± 0.547
2.15LysPro: 2.15 ± 0.45
2.834LysGln: 2.834 ± 0.57
4.104LysArg: 4.104 ± 0.519
4.104LysSer: 4.104 ± 0.657
2.736LysThr: 2.736 ± 0.457
3.322LysVal: 3.322 ± 0.728
0.782LysTrp: 0.782 ± 0.277
1.368LysTyr: 1.368 ± 0.434
0.0LysXaa: 0.0 ± 0.0
Leu
6.058LeuAla: 6.058 ± 0.814
0.782LeuCys: 0.782 ± 0.313
6.449LeuAsp: 6.449 ± 0.701
4.886LeuGlu: 4.886 ± 0.77
2.15LeuPhe: 2.15 ± 0.444
4.886LeuGly: 4.886 ± 0.544
1.661LeuHis: 1.661 ± 0.385
3.909LeuIle: 3.909 ± 0.666
4.397LeuLys: 4.397 ± 0.893
4.886LeuLeu: 4.886 ± 0.675
2.052LeuMet: 2.052 ± 0.533
4.104LeuAsn: 4.104 ± 0.673
2.736LeuPro: 2.736 ± 0.283
2.443LeuGln: 2.443 ± 0.335
4.104LeuArg: 4.104 ± 0.55
5.179LeuSer: 5.179 ± 0.571
5.277LeuThr: 5.277 ± 0.775
4.495LeuVal: 4.495 ± 0.709
0.879LeuTrp: 0.879 ± 0.32
2.834LeuTyr: 2.834 ± 0.424
0.0LeuXaa: 0.0 ± 0.0
Met
2.443MetAla: 2.443 ± 0.43
0.684MetCys: 0.684 ± 0.299
1.661MetAsp: 1.661 ± 0.392
1.075MetGlu: 1.075 ± 0.294
1.466MetPhe: 1.466 ± 0.352
1.368MetGly: 1.368 ± 0.298
0.391MetHis: 0.391 ± 0.145
1.173MetIle: 1.173 ± 0.344
0.782MetLys: 0.782 ± 0.291
1.954MetLeu: 1.954 ± 0.422
0.684MetMet: 0.684 ± 0.237
1.759MetAsn: 1.759 ± 0.494
1.173MetPro: 1.173 ± 0.3
1.075MetGln: 1.075 ± 0.307
3.127MetArg: 3.127 ± 0.645
1.661MetSer: 1.661 ± 0.463
2.541MetThr: 2.541 ± 0.452
1.466MetVal: 1.466 ± 0.447
0.391MetTrp: 0.391 ± 0.204
0.684MetTyr: 0.684 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
4.104AsnAla: 4.104 ± 0.644
0.879AsnCys: 0.879 ± 0.316
2.638AsnAsp: 2.638 ± 0.503
1.466AsnGlu: 1.466 ± 0.368
1.759AsnPhe: 1.759 ± 0.454
4.886AsnGly: 4.886 ± 0.891
1.368AsnHis: 1.368 ± 0.332
2.443AsnIle: 2.443 ± 0.551
2.736AsnLys: 2.736 ± 0.575
2.834AsnLeu: 2.834 ± 0.576
1.27AsnMet: 1.27 ± 0.429
3.322AsnAsn: 3.322 ± 0.902
2.931AsnPro: 2.931 ± 0.646
1.368AsnGln: 1.368 ± 0.362
2.15AsnArg: 2.15 ± 0.581
3.909AsnSer: 3.909 ± 0.813
2.931AsnThr: 2.931 ± 0.628
3.811AsnVal: 3.811 ± 0.441
0.879AsnTrp: 0.879 ± 0.304
1.759AsnTyr: 1.759 ± 0.407
0.0AsnXaa: 0.0 ± 0.0
Pro
3.811ProAla: 3.811 ± 0.493
0.293ProCys: 0.293 ± 0.156
2.541ProAsp: 2.541 ± 0.331
2.15ProGlu: 2.15 ± 0.443
1.466ProPhe: 1.466 ± 0.331
3.518ProGly: 3.518 ± 0.683
0.586ProHis: 0.586 ± 0.206
1.857ProIle: 1.857 ± 0.439
2.052ProLys: 2.052 ± 0.477
3.42ProLeu: 3.42 ± 0.445
0.782ProMet: 0.782 ± 0.245
2.247ProAsn: 2.247 ± 0.337
1.563ProPro: 1.563 ± 0.333
1.563ProGln: 1.563 ± 0.298
1.857ProArg: 1.857 ± 0.306
2.345ProSer: 2.345 ± 0.383
1.857ProThr: 1.857 ± 0.43
3.713ProVal: 3.713 ± 0.624
0.684ProTrp: 0.684 ± 0.23
1.27ProTyr: 1.27 ± 0.265
0.0ProXaa: 0.0 ± 0.0
Gln
2.834GlnAla: 2.834 ± 0.613
0.489GlnCys: 0.489 ± 0.197
1.759GlnAsp: 1.759 ± 0.368
2.541GlnGlu: 2.541 ± 0.436
1.857GlnPhe: 1.857 ± 0.435
2.541GlnGly: 2.541 ± 0.505
0.586GlnHis: 0.586 ± 0.199
2.736GlnIle: 2.736 ± 0.638
2.052GlnLys: 2.052 ± 0.566
4.788GlnLeu: 4.788 ± 0.752
1.563GlnMet: 1.563 ± 0.446
1.857GlnAsn: 1.857 ± 0.513
0.977GlnPro: 0.977 ± 0.254
2.834GlnGln: 2.834 ± 0.497
2.345GlnArg: 2.345 ± 0.539
3.42GlnSer: 3.42 ± 0.576
3.713GlnThr: 3.713 ± 0.632
2.541GlnVal: 2.541 ± 0.452
0.684GlnTrp: 0.684 ± 0.278
1.466GlnTyr: 1.466 ± 0.321
0.0GlnXaa: 0.0 ± 0.0
Arg
4.495ArgAla: 4.495 ± 0.583
0.586ArgCys: 0.586 ± 0.207
3.029ArgAsp: 3.029 ± 0.652
3.713ArgGlu: 3.713 ± 0.71
1.466ArgPhe: 1.466 ± 0.403
2.638ArgGly: 2.638 ± 0.49
1.173ArgHis: 1.173 ± 0.338
3.42ArgIle: 3.42 ± 0.549
2.834ArgLys: 2.834 ± 0.535
4.886ArgLeu: 4.886 ± 0.673
1.075ArgMet: 1.075 ± 0.293
2.638ArgAsn: 2.638 ± 0.343
2.638ArgPro: 2.638 ± 0.49
3.029ArgGln: 3.029 ± 0.684
2.541ArgArg: 2.541 ± 0.453
3.127ArgSer: 3.127 ± 0.413
2.443ArgThr: 2.443 ± 0.43
4.299ArgVal: 4.299 ± 0.677
1.563ArgTrp: 1.563 ± 0.386
2.345ArgTyr: 2.345 ± 0.505
0.0ArgXaa: 0.0 ± 0.0
Ser
5.667SerAla: 5.667 ± 1.111
0.391SerCys: 0.391 ± 0.203
3.811SerAsp: 3.811 ± 0.59
2.736SerGlu: 2.736 ± 0.511
2.541SerPhe: 2.541 ± 0.549
5.374SerGly: 5.374 ± 0.589
1.466SerHis: 1.466 ± 0.523
3.225SerIle: 3.225 ± 0.527
4.495SerLys: 4.495 ± 0.582
4.593SerLeu: 4.593 ± 0.577
2.15SerMet: 2.15 ± 0.525
2.736SerAsn: 2.736 ± 0.542
2.247SerPro: 2.247 ± 0.514
3.42SerGln: 3.42 ± 0.495
2.736SerArg: 2.736 ± 0.594
4.593SerSer: 4.593 ± 1.128
4.299SerThr: 4.299 ± 0.804
3.322SerVal: 3.322 ± 0.508
0.586SerTrp: 0.586 ± 0.232
2.345SerTyr: 2.345 ± 0.443
0.0SerXaa: 0.0 ± 0.0
Thr
4.886ThrAla: 4.886 ± 0.705
1.173ThrCys: 1.173 ± 0.354
3.713ThrAsp: 3.713 ± 0.637
3.909ThrGlu: 3.909 ± 0.642
2.541ThrPhe: 2.541 ± 0.571
6.058ThrGly: 6.058 ± 0.798
1.954ThrHis: 1.954 ± 0.559
4.104ThrIle: 4.104 ± 0.534
3.225ThrLys: 3.225 ± 0.623
4.495ThrLeu: 4.495 ± 0.554
1.27ThrMet: 1.27 ± 0.324
2.443ThrAsn: 2.443 ± 0.494
4.495ThrPro: 4.495 ± 0.603
2.247ThrGln: 2.247 ± 0.441
2.541ThrArg: 2.541 ± 0.387
4.397ThrSer: 4.397 ± 0.66
4.886ThrThr: 4.886 ± 0.915
5.765ThrVal: 5.765 ± 1.045
1.173ThrTrp: 1.173 ± 0.424
1.954ThrTyr: 1.954 ± 0.42
0.0ThrXaa: 0.0 ± 0.0
Val
5.961ValAla: 5.961 ± 0.956
0.586ValCys: 0.586 ± 0.184
5.179ValAsp: 5.179 ± 0.844
4.299ValGlu: 4.299 ± 0.728
3.029ValPhe: 3.029 ± 0.634
3.811ValGly: 3.811 ± 0.59
1.466ValHis: 1.466 ± 0.472
3.42ValIle: 3.42 ± 0.597
4.006ValLys: 4.006 ± 0.527
4.397ValLeu: 4.397 ± 0.548
2.443ValMet: 2.443 ± 0.44
4.006ValAsn: 4.006 ± 0.534
3.322ValPro: 3.322 ± 0.581
3.029ValGln: 3.029 ± 0.517
3.42ValArg: 3.42 ± 0.564
3.322ValSer: 3.322 ± 0.476
7.915ValThr: 7.915 ± 0.953
3.225ValVal: 3.225 ± 0.522
0.879ValTrp: 0.879 ± 0.297
2.638ValTyr: 2.638 ± 0.429
0.0ValXaa: 0.0 ± 0.0
Trp
1.075TrpAla: 1.075 ± 0.309
0.195TrpCys: 0.195 ± 0.121
0.977TrpAsp: 0.977 ± 0.213
0.195TrpGlu: 0.195 ± 0.127
0.684TrpPhe: 0.684 ± 0.231
0.977TrpGly: 0.977 ± 0.321
0.293TrpHis: 0.293 ± 0.156
1.075TrpIle: 1.075 ± 0.379
1.27TrpLys: 1.27 ± 0.412
1.661TrpLeu: 1.661 ± 0.344
0.098TrpMet: 0.098 ± 0.098
0.684TrpAsn: 0.684 ± 0.241
0.684TrpPro: 0.684 ± 0.277
0.782TrpGln: 0.782 ± 0.314
0.684TrpArg: 0.684 ± 0.256
0.586TrpSer: 0.586 ± 0.25
0.977TrpThr: 0.977 ± 0.314
1.368TrpVal: 1.368 ± 0.362
0.0TrpTrp: 0.0 ± 0.0
0.684TrpTyr: 0.684 ± 0.284
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.834TyrAla: 2.834 ± 0.329
0.782TyrCys: 0.782 ± 0.3
2.541TyrAsp: 2.541 ± 0.617
1.661TyrGlu: 1.661 ± 0.453
1.075TyrPhe: 1.075 ± 0.306
2.931TyrGly: 2.931 ± 0.517
0.391TyrHis: 0.391 ± 0.215
1.075TyrIle: 1.075 ± 0.279
2.443TyrLys: 2.443 ± 0.392
2.931TyrLeu: 2.931 ± 0.543
1.075TyrMet: 1.075 ± 0.28
2.247TyrAsn: 2.247 ± 0.491
1.173TyrPro: 1.173 ± 0.416
1.466TyrGln: 1.466 ± 0.29
1.857TyrArg: 1.857 ± 0.486
2.443TyrSer: 2.443 ± 0.426
2.443TyrThr: 2.443 ± 0.377
3.029TyrVal: 3.029 ± 0.481
0.489TyrTrp: 0.489 ± 0.213
1.075TyrTyr: 1.075 ± 0.309
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 35 proteins (10235 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski