Tortoise microvirus 5

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W5M6|A0A4P8W5M6_9VIRU Uncharacterized protein OS=Tortoise microvirus 5 OX=2583154 PE=4 SV=1
MM1 pKa = 6.9KK2 pKa = 9.93TNVYY6 pKa = 10.43SIIDD10 pKa = 3.53SAYY13 pKa = 9.38GRR15 pKa = 11.84IQGVVVAPNSSLAIRR30 pKa = 11.84DD31 pKa = 3.63VAPMLKK37 pKa = 9.63RR38 pKa = 11.84VAPHH42 pKa = 6.49FEE44 pKa = 3.4EE45 pKa = 5.9DD46 pKa = 3.83MYY48 pKa = 11.12IEE50 pKa = 4.83CIGYY54 pKa = 10.24YY55 pKa = 10.42DD56 pKa = 5.74DD57 pKa = 5.96DD58 pKa = 5.67LNFHH62 pKa = 6.11ATSPEE67 pKa = 4.18VYY69 pKa = 9.28PWLADD74 pKa = 3.65NPEE77 pKa = 4.25MPMKK81 pKa = 10.17TLTGEE86 pKa = 3.92EE87 pKa = 4.01AVII90 pKa = 4.28

Molecular weight:
10.13 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P8W5R7|A0A4P8W5R7_9VIRU Uncharacterized protein OS=Tortoise microvirus 5 OX=2583154 PE=4 SV=1
MM1 pKa = 7.15LQHH4 pKa = 6.8HH5 pKa = 6.04SHH7 pKa = 7.28LIKK10 pKa = 10.69GVFSMRR16 pKa = 11.84KK17 pKa = 7.7TITTSISIPKK27 pKa = 9.35DD28 pKa = 3.65LKK30 pKa = 10.94EE31 pKa = 3.75KK32 pKa = 10.01WIDD35 pKa = 3.35KK36 pKa = 9.65MSISISYY43 pKa = 7.55FTSCAMKK50 pKa = 10.44SVLRR54 pKa = 11.84NPALLEE60 pKa = 4.24TILFQNSLKK69 pKa = 10.68EE70 pKa = 3.95FF71 pKa = 4.03

Molecular weight:
8.21 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1659

71

627

237.0

26.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.691 ± 0.705

1.206 ± 0.469

6.57 ± 1.132

4.159 ± 0.505

4.882 ± 0.612

6.45 ± 0.809

1.567 ± 0.445

5.726 ± 0.48

5.184 ± 0.903

8.74 ± 0.873

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.389

5.063 ± 0.404

4.28 ± 0.82

3.918 ± 1.086

5.726 ± 0.877

10.488 ± 0.838

5.546 ± 0.674

5.003 ± 0.507

1.206 ± 0.287

5.003 ± 0.739

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski