Gymnopus luxurians FD-317 M1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Omphalotaceae; Gymnopus; Gymnopus luxurians

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D0CGC0|A0A0D0CGC0_9AGAR Unplaced genomic scaffold GYMLUscaffold_22 whole genome shotgun sequence (Fragment) OS=Gymnopus luxurians FD-317 M1 OX=944289 GN=GYMLUDRAFT_166192 PE=3 SV=1
MM1 pKa = 7.21ATHH4 pKa = 6.5PHH6 pKa = 6.61VMMFLAGSAVLARR19 pKa = 11.84NAQSNSDD26 pKa = 3.62QLLSLSFEE34 pKa = 4.18LWLQVLDD41 pKa = 4.79NEE43 pKa = 4.61VLNDD47 pKa = 3.38NAIDD51 pKa = 3.99FFYY54 pKa = 11.16TNIDD58 pKa = 4.57DD59 pKa = 4.61ILDD62 pKa = 3.62SSTAYY67 pKa = 9.43DD68 pKa = 3.54TVSSVNAHH76 pKa = 5.94VDD78 pKa = 3.84DD79 pKa = 4.67MSLDD83 pKa = 3.55TAMEE87 pKa = 4.02MSNTGMDD94 pKa = 4.2SLWLFINPLMIEE106 pKa = 4.22TSSNLAQLANAYY118 pKa = 8.74TEE120 pKa = 5.02LDD122 pKa = 3.75LFQAPLEE129 pKa = 4.38AAGDD133 pKa = 4.15FYY135 pKa = 11.58FSFVPYY141 pKa = 9.6WIHH144 pKa = 7.14LRR146 pKa = 11.84VLLEE150 pKa = 3.78AEE152 pKa = 4.23IPVAAGVSSAPQATKK167 pKa = 10.82

Molecular weight: 18.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D0CAZ8|A0A0D0CAZ8_9AGAR Unplaced genomic scaffold GYMLUscaffold_30 whole genome shotgun sequence OS=Gymnopus luxurians FD-317 M1 OX=944289 GN=GYMLUDRAFT_44096 PE=4 SV=1
MM1 pKa = 7.75TYY3 pKa = 10.19RR4 pKa = 11.84PSSLNVQQPHH14 pKa = 5.62TSAARR19 pKa = 11.84PLTVPVHH26 pKa = 6.2RR27 pKa = 11.84PSIFSFSLFFHH38 pKa = 5.08QHH40 pKa = 4.5EE41 pKa = 4.95SEE43 pKa = 4.37LACLPSKK50 pKa = 10.42IRR52 pKa = 11.84EE53 pKa = 4.03RR54 pKa = 11.84WPSHH58 pKa = 4.61ARR60 pKa = 11.84PRR62 pKa = 11.84QRR64 pKa = 11.84LCLDD68 pKa = 4.48LARR71 pKa = 11.84KK72 pKa = 9.37ICGSTLRR79 pKa = 11.84CDD81 pKa = 3.45EE82 pKa = 4.66ACAQRR87 pKa = 11.84ATKK90 pKa = 10.56LSTVPARR97 pKa = 11.84FLNSYY102 pKa = 7.27TSRR105 pKa = 11.84DD106 pKa = 3.59YY107 pKa = 11.66NRR109 pKa = 11.84TKK111 pKa = 10.39SEE113 pKa = 3.74RR114 pKa = 11.84RR115 pKa = 11.84EE116 pKa = 3.87VLSFVPQNPGEE127 pKa = 4.04RR128 pKa = 11.84LNRR131 pKa = 11.84IQAGWQNLEE140 pKa = 4.23YY141 pKa = 10.79NNSPFLSLRR150 pKa = 11.84HH151 pKa = 5.42SFGMDD156 pKa = 2.62RR157 pKa = 11.84KK158 pKa = 9.96LDD160 pKa = 3.6RR161 pKa = 11.84SRR163 pKa = 11.84CPFGMMNRR171 pKa = 11.84SWSAEE176 pKa = 3.8EE177 pKa = 3.71PSFTFRR183 pKa = 11.84QNMM186 pKa = 3.33

Molecular weight: 21.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14578

10

14588

4980570

49

3064

341.4

37.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.667 ± 0.021

1.419 ± 0.01

5.312 ± 0.012

5.724 ± 0.024

4.086 ± 0.014

6.453 ± 0.026

2.569 ± 0.01

5.392 ± 0.018

4.583 ± 0.017

9.481 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.106 ± 0.008

4.001 ± 0.014

6.018 ± 0.025

3.873 ± 0.015

5.324 ± 0.023

9.416 ± 0.032

6.017 ± 0.018

6.236 ± 0.017

1.53 ± 0.009

2.795 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski