Bacillus aerolatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I1FPK2|A0A6I1FPK2_9BACI Class II aldolase/adducin family protein OS=Bacillus aerolatus OX=2653354 GN=F9802_11530 PE=4 SV=1
MM1 pKa = 7.62QSQNLQGAQEE11 pKa = 4.14LLCINAEE18 pKa = 4.12KK19 pKa = 10.78VYY21 pKa = 10.98DD22 pKa = 3.76WVILQNTVNQNVLAGALGLPAGFNPCDD49 pKa = 3.53PSVTNLTTRR58 pKa = 11.84CFLVDD63 pKa = 3.79EE64 pKa = 5.73DD65 pKa = 5.11GDD67 pKa = 4.13PLPVNAEE74 pKa = 4.08VEE76 pKa = 4.22VEE78 pKa = 4.2EE79 pKa = 4.48IEE81 pKa = 4.18EE82 pKa = 4.37RR83 pKa = 11.84EE84 pKa = 4.1DD85 pKa = 3.19RR86 pKa = 11.84TFVIDD91 pKa = 3.75GALVTLQRR99 pKa = 11.84VTFLKK104 pKa = 9.46PLCVRR109 pKa = 11.84VEE111 pKa = 3.88ISGVAGTTPFVGVTDD126 pKa = 5.1PIGIEE131 pKa = 3.72IPEE134 pKa = 4.45SVFLCAPEE142 pKa = 4.13GTRR145 pKa = 11.84LVVRR149 pKa = 11.84LTDD152 pKa = 3.12VDD154 pKa = 3.68CSVRR158 pKa = 11.84INCTGTDD165 pKa = 3.82FTSIDD170 pKa = 3.26IALNLCQSIQTVADD184 pKa = 3.43VTLEE188 pKa = 4.07LEE190 pKa = 4.58ADD192 pKa = 3.47FCEE195 pKa = 4.52PRR197 pKa = 11.84DD198 pKa = 3.94ILIEE202 pKa = 3.92QCPAPVIPPQCPVLFPGIPGEE223 pKa = 4.12

Molecular weight: 24.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I1FN28|A0A6I1FN28_9BACI ATP-binding cassette domain-containing protein OS=Bacillus aerolatus OX=2653354 GN=F9802_00505 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.02NGRR28 pKa = 11.84RR29 pKa = 11.84ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3659

0

3659

1091206

25

1529

298.2

33.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.972 ± 0.045

0.735 ± 0.013

4.794 ± 0.03

7.779 ± 0.055

4.544 ± 0.034

7.135 ± 0.04

2.079 ± 0.019

7.406 ± 0.039

6.849 ± 0.043

9.761 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.02

3.98 ± 0.025

3.76 ± 0.023

3.723 ± 0.028

4.138 ± 0.031

5.891 ± 0.026

5.295 ± 0.027

7.102 ± 0.032

0.999 ± 0.016

3.232 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski