Amino acid dipepetide frequency for Bacillus aerolatus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.449AlaAla: 8.449 ± 0.117	0.696AlaCys: 0.696 ± 0.025	3.969AlaAsp: 3.969 ± 0.064	5.818AlaGlu: 5.818 ± 0.08	3.691AlaPhe: 3.691 ± 0.061	6.72AlaGly: 6.72 ± 0.1	1.385AlaHis: 1.385 ± 0.034	6.044AlaIle: 6.044 ± 0.075	5.021AlaLys: 5.021 ± 0.071	7.774AlaLeu: 7.774 ± 0.088	2.154AlaMet: 2.154 ± 0.043	2.816AlaAsn: 2.816 ± 0.056	2.41AlaPro: 2.41 ± 0.061	2.394AlaGln: 2.394 ± 0.046	3.008AlaArg: 3.008 ± 0.051	4.498AlaSer: 4.498 ± 0.06	3.264AlaThr: 3.264 ± 0.059	6.664AlaVal: 6.664 ± 0.085	0.64AlaTrp: 0.64 ± 0.028	2.306AlaTyr: 2.306 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.511CysAla: 0.511 ± 0.024	0.088CysCys: 0.088 ± 0.009	0.38CysAsp: 0.38 ± 0.021	0.438CysGlu: 0.438 ± 0.021	0.342CysPhe: 0.342 ± 0.021	0.732CysGly: 0.732 ± 0.03	0.193CysHis: 0.193 ± 0.013	0.487CysIle: 0.487 ± 0.018	0.351CysLys: 0.351 ± 0.02	0.697CysLeu: 0.697 ± 0.028	0.184CysMet: 0.184 ± 0.011	0.267CysAsn: 0.267 ± 0.018	0.384CysPro: 0.384 ± 0.021	0.288CysGln: 0.288 ± 0.016	0.346CysArg: 0.346 ± 0.021	0.499CysSer: 0.499 ± 0.022	0.423CysThr: 0.423 ± 0.021	0.406CysVal: 0.406 ± 0.019	0.074CysTrp: 0.074 ± 0.009	0.26CysTyr: 0.26 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.269AspAla: 3.269 ± 0.058	0.393AspCys: 0.393 ± 0.019	2.095AspAsp: 2.095 ± 0.047	4.279AspGlu: 4.279 ± 0.077	2.32AspPhe: 2.32 ± 0.045	3.186AspGly: 3.186 ± 0.053	1.179AspHis: 1.179 ± 0.032	3.929AspIle: 3.929 ± 0.067	2.799AspLys: 2.799 ± 0.056	4.745AspLeu: 4.745 ± 0.07	1.365AspMet: 1.365 ± 0.034	1.526AspAsn: 1.526 ± 0.038	1.995AspPro: 1.995 ± 0.039	1.831AspGln: 1.831 ± 0.049	2.171AspArg: 2.171 ± 0.043	2.418AspSer: 2.418 ± 0.047	2.227AspThr: 2.227 ± 0.045	3.65AspVal: 3.65 ± 0.07	0.606AspTrp: 0.606 ± 0.026	1.96AspTyr: 1.96 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.439GluAla: 6.439 ± 0.087	0.385GluCys: 0.385 ± 0.021	3.459GluAsp: 3.459 ± 0.056	8.048GluGlu: 8.048 ± 0.124	2.463GluPhe: 2.463 ± 0.052	4.682GluGly: 4.682 ± 0.073	1.45GluHis: 1.45 ± 0.039	5.045GluIle: 5.045 ± 0.073	7.379GluLys: 7.379 ± 0.105	7.363GluLeu: 7.363 ± 0.084	2.385GluMet: 2.385 ± 0.052	3.327GluAsn: 3.327 ± 0.06	2.057GluPro: 2.057 ± 0.047	3.569GluGln: 3.569 ± 0.056	3.83GluArg: 3.83 ± 0.063	3.556GluSer: 3.556 ± 0.056	4.156GluThr: 4.156 ± 0.067	5.262GluVal: 5.262 ± 0.073	0.895GluTrp: 0.895 ± 0.031	2.038GluTyr: 2.038 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.361PheAla: 3.361 ± 0.059	0.343PheCys: 0.343 ± 0.018	2.206PheAsp: 2.206 ± 0.04	2.752PheGlu: 2.752 ± 0.046	2.546PhePhe: 2.546 ± 0.062	3.43PheGly: 3.43 ± 0.06	1.025PheHis: 1.025 ± 0.033	3.748PheIle: 3.748 ± 0.072	2.367PheLys: 2.367 ± 0.051	4.644PheLeu: 4.644 ± 0.081	1.188PheMet: 1.188 ± 0.04	1.749PheAsn: 1.749 ± 0.041	1.732PhePro: 1.732 ± 0.039	1.577PheGln: 1.577 ± 0.04	1.532PheArg: 1.532 ± 0.038	3.347PheSer: 3.347 ± 0.057	2.661PheThr: 2.661 ± 0.053	3.166PheVal: 3.166 ± 0.052	0.444PheTrp: 0.444 ± 0.023	1.624PheTyr: 1.624 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	5.657GlyAla: 5.657 ± 0.098	0.653GlyCys: 0.653 ± 0.024	3.073GlyAsp: 3.073 ± 0.052	4.869GlyGlu: 4.869 ± 0.079	3.497GlyPhe: 3.497 ± 0.068	5.265GlyGly: 5.265 ± 0.089	1.418GlyHis: 1.418 ± 0.039	5.697GlyIle: 5.697 ± 0.074	5.257GlyLys: 5.257 ± 0.064	6.981GlyLeu: 6.981 ± 0.095	2.291GlyMet: 2.291 ± 0.048	2.46GlyAsn: 2.46 ± 0.049	1.891GlyPro: 1.891 ± 0.037	2.348GlyGln: 2.348 ± 0.05	3.013GlyArg: 3.013 ± 0.056	4.129GlySer: 4.129 ± 0.057	4.205GlyThr: 4.205 ± 0.058	5.242GlyVal: 5.242 ± 0.076	0.839GlyTrp: 0.839 ± 0.026	2.564GlyTyr: 2.564 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.375HisAla: 1.375 ± 0.035	0.205HisCys: 0.205 ± 0.014	0.956HisAsp: 0.956 ± 0.03	1.457HisGlu: 1.457 ± 0.036	1.13HisPhe: 1.13 ± 0.036	1.476HisGly: 1.476 ± 0.041	0.651HisHis: 0.651 ± 0.027	1.414HisIle: 1.414 ± 0.036	0.953HisLys: 0.953 ± 0.027	2.198HisLeu: 2.198 ± 0.039	0.537HisMet: 0.537 ± 0.023	0.701HisAsn: 0.701 ± 0.026	1.257HisPro: 1.257 ± 0.037	0.765HisGln: 0.765 ± 0.025	0.894HisArg: 0.894 ± 0.028	1.315HisSer: 1.315 ± 0.035	1.084HisThr: 1.084 ± 0.035	1.437HisVal: 1.437 ± 0.04	0.204HisTrp: 0.204 ± 0.015	0.778HisTyr: 0.778 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.147IleAla: 6.147 ± 0.084	0.628IleCys: 0.628 ± 0.024	4.035IleAsp: 4.035 ± 0.06	5.534IleGlu: 5.534 ± 0.078	3.143IlePhe: 3.143 ± 0.058	5.973IleGly: 5.973 ± 0.077	1.676IleHis: 1.676 ± 0.04	5.488IleIle: 5.488 ± 0.083	4.064IleLys: 4.064 ± 0.062	6.463IleLeu: 6.463 ± 0.09	1.724IleMet: 1.724 ± 0.041	3.009IleAsn: 3.009 ± 0.062	3.174IlePro: 3.174 ± 0.052	2.885IleGln: 2.885 ± 0.051	3.109IleArg: 3.109 ± 0.053	4.676IleSer: 4.676 ± 0.071	4.011IleThr: 4.011 ± 0.061	5.518IleVal: 5.518 ± 0.066	0.591IleTrp: 0.591 ± 0.023	2.212IleTyr: 2.212 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	5.258LysAla: 5.258 ± 0.072	0.289LysCys: 0.289 ± 0.017	3.613LysAsp: 3.613 ± 0.053	7.417LysGlu: 7.417 ± 0.102	1.841LysPhe: 1.841 ± 0.043	4.406LysGly: 4.406 ± 0.068	1.224LysHis: 1.224 ± 0.034	4.262LysIle: 4.262 ± 0.07	6.121LysLys: 6.121 ± 0.091	5.58LysLeu: 5.58 ± 0.079	2.253LysMet: 2.253 ± 0.045	3.099LysAsn: 3.099 ± 0.053	2.23LysPro: 2.23 ± 0.047	3.199LysGln: 3.199 ± 0.066	3.337LysArg: 3.337 ± 0.062	3.279LysSer: 3.279 ± 0.062	3.541LysThr: 3.541 ± 0.056	4.727LysVal: 4.727 ± 0.063	0.839LysTrp: 0.839 ± 0.032	1.968LysTyr: 1.968 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	8.323LeuAla: 8.323 ± 0.092	0.641LeuCys: 0.641 ± 0.027	4.509LeuAsp: 4.509 ± 0.072	6.491LeuGlu: 6.491 ± 0.085	4.945LeuPhe: 4.945 ± 0.075	6.386LeuGly: 6.386 ± 0.089	1.993LeuHis: 1.993 ± 0.048	7.177LeuIle: 7.177 ± 0.098	6.53LeuLys: 6.53 ± 0.084	10.248LeuLeu: 10.248 ± 0.13	2.552LeuMet: 2.552 ± 0.05	4.034LeuAsn: 4.034 ± 0.062	4.177LeuPro: 4.177 ± 0.062	3.779LeuGln: 3.779 ± 0.061	3.415LeuArg: 3.415 ± 0.056	6.836LeuSer: 6.836 ± 0.088	5.655LeuThr: 5.655 ± 0.073	6.025LeuVal: 6.025 ± 0.081	0.818LeuTrp: 0.818 ± 0.027	3.076LeuTyr: 3.076 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.378MetAla: 2.378 ± 0.048	0.163MetCys: 0.163 ± 0.015	1.416MetAsp: 1.416 ± 0.035	2.135MetGlu: 2.135 ± 0.043	1.03MetPhe: 1.03 ± 0.037	1.779MetGly: 1.779 ± 0.045	0.425MetHis: 0.425 ± 0.021	2.187MetIle: 2.187 ± 0.038	2.615MetLys: 2.615 ± 0.047	2.518MetLeu: 2.518 ± 0.055	0.89MetMet: 0.89 ± 0.033	1.574MetAsn: 1.574 ± 0.037	1.103MetPro: 1.103 ± 0.034	0.938MetGln: 0.938 ± 0.03	1.06MetArg: 1.06 ± 0.029	1.643MetSer: 1.643 ± 0.034	1.783MetThr: 1.783 ± 0.039	1.698MetVal: 1.698 ± 0.043	0.196MetTrp: 0.196 ± 0.013	0.724MetTyr: 0.724 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.482AsnAla: 2.482 ± 0.054	0.344AsnCys: 0.344 ± 0.021	1.978AsnAsp: 1.978 ± 0.046	3.404AsnGlu: 3.404 ± 0.059	1.467AsnPhe: 1.467 ± 0.035	3.103AsnGly: 3.103 ± 0.064	0.9AsnHis: 0.9 ± 0.031	3.125AsnIle: 3.125 ± 0.061	2.782AsnLys: 2.782 ± 0.061	3.42AsnLeu: 3.42 ± 0.058	1.208AsnMet: 1.208 ± 0.035	1.716AsnAsn: 1.716 ± 0.049	1.902AsnPro: 1.902 ± 0.042	1.49AsnGln: 1.49 ± 0.049	1.871AsnArg: 1.871 ± 0.043	2.09AsnSer: 2.09 ± 0.045	1.869AsnThr: 1.869 ± 0.042	2.823AsnVal: 2.823 ± 0.061	0.496AsnTrp: 0.496 ± 0.02	1.332AsnTyr: 1.332 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.074ProAla: 3.074 ± 0.059	0.236ProCys: 0.236 ± 0.015	2.094ProAsp: 2.094 ± 0.049	3.232ProGlu: 3.232 ± 0.06	2.086ProPhe: 2.086 ± 0.048	2.652ProGly: 2.652 ± 0.054	0.884ProHis: 0.884 ± 0.033	2.555ProIle: 2.555 ± 0.055	2.037ProLys: 2.037 ± 0.046	3.888ProLeu: 3.888 ± 0.058	0.854ProMet: 0.854 ± 0.029	1.483ProAsn: 1.483 ± 0.041	1.207ProPro: 1.207 ± 0.033	1.151ProGln: 1.151 ± 0.031	1.139ProArg: 1.139 ± 0.033	2.274ProSer: 2.274 ± 0.044	1.727ProThr: 1.727 ± 0.039	3.259ProVal: 3.259 ± 0.062	0.367ProTrp: 0.367 ± 0.021	1.399ProTyr: 1.399 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	3.103GlnAla: 3.103 ± 0.049	0.214GlnCys: 0.214 ± 0.014	1.424GlnAsp: 1.424 ± 0.038	2.961GlnGlu: 2.961 ± 0.054	1.7GlnPhe: 1.7 ± 0.036	2.156GlnGly: 2.156 ± 0.048	0.767GlnHis: 0.767 ± 0.026	2.395GlnIle: 2.395 ± 0.054	2.669GlnLys: 2.669 ± 0.05	4.132GlnLeu: 4.132 ± 0.073	1.2GlnMet: 1.2 ± 0.034	1.314GlnAsn: 1.314 ± 0.038	1.458GlnPro: 1.458 ± 0.04	1.954GlnGln: 1.954 ± 0.05	1.566GlnArg: 1.566 ± 0.038	2.204GlnSer: 2.204 ± 0.054	2.062GlnThr: 2.062 ± 0.042	2.381GlnVal: 2.381 ± 0.05	0.394GlnTrp: 0.394 ± 0.019	1.177GlnTyr: 1.177 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.788ArgAla: 2.788 ± 0.06	0.28ArgCys: 0.28 ± 0.02	1.874ArgAsp: 1.874 ± 0.05	3.473ArgGlu: 3.473 ± 0.064	1.981ArgPhe: 1.981 ± 0.044	2.568ArgGly: 2.568 ± 0.052	0.927ArgHis: 0.927 ± 0.03	2.941ArgIle: 2.941 ± 0.053	3.159ArgLys: 3.159 ± 0.058	4.381ArgLeu: 4.381 ± 0.072	1.296ArgMet: 1.296 ± 0.038	1.628ArgAsn: 1.628 ± 0.042	1.499ArgPro: 1.499 ± 0.04	1.694ArgGln: 1.694 ± 0.039	2.005ArgArg: 2.005 ± 0.052	2.286ArgSer: 2.286 ± 0.042	2.176ArgThr: 2.176 ± 0.05	2.592ArgVal: 2.592 ± 0.054	0.398ArgTrp: 0.398 ± 0.019	1.428ArgTyr: 1.428 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	4.336SerAla: 4.336 ± 0.072	0.42SerCys: 0.42 ± 0.019	2.529SerAsp: 2.529 ± 0.055	3.854SerGlu: 3.854 ± 0.063	3.299SerPhe: 3.299 ± 0.057	4.83SerGly: 4.83 ± 0.069	1.249SerHis: 1.249 ± 0.035	4.565SerIle: 4.565 ± 0.064	3.508SerLys: 3.508 ± 0.063	6.237SerLeu: 6.237 ± 0.086	1.706SerMet: 1.706 ± 0.041	2.13SerAsn: 2.13 ± 0.045	2.281SerPro: 2.281 ± 0.049	1.9SerGln: 1.9 ± 0.043	2.525SerArg: 2.525 ± 0.048	3.842SerSer: 3.842 ± 0.062	2.921SerThr: 2.921 ± 0.055	4.059SerVal: 4.059 ± 0.061	0.632SerTrp: 0.632 ± 0.026	2.089SerTyr: 2.089 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	4.896ThrAla: 4.896 ± 0.071	0.384ThrCys: 0.384 ± 0.021	2.646ThrAsp: 2.646 ± 0.052	3.612ThrGlu: 3.612 ± 0.057	2.56ThrPhe: 2.56 ± 0.05	4.42ThrGly: 4.42 ± 0.063	1.011ThrHis: 1.011 ± 0.029	4.158ThrIle: 4.158 ± 0.066	3.321ThrLys: 3.321 ± 0.056	4.873ThrLeu: 4.873 ± 0.067	1.335ThrMet: 1.335 ± 0.034	2.144ThrAsn: 2.144 ± 0.046	2.315ThrPro: 2.315 ± 0.05	1.291ThrGln: 1.291 ± 0.038	1.858ThrArg: 1.858 ± 0.042	2.888ThrSer: 2.888 ± 0.051	2.755ThrThr: 2.755 ± 0.056	4.373ThrVal: 4.373 ± 0.072	0.448ThrTrp: 0.448 ± 0.02	1.663ThrTyr: 1.663 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	5.036ValAla: 5.036 ± 0.072	0.593ValCys: 0.593 ± 0.025	3.522ValAsp: 3.522 ± 0.059	4.871ValGlu: 4.871 ± 0.08	3.288ValPhe: 3.288 ± 0.063	4.477ValGly: 4.477 ± 0.064	1.474ValHis: 1.474 ± 0.038	5.902ValIle: 5.902 ± 0.085	4.831ValLys: 4.831 ± 0.07	7.064ValLeu: 7.064 ± 0.084	1.956ValMet: 1.956 ± 0.048	3.078ValAsn: 3.078 ± 0.058	2.889ValPro: 2.889 ± 0.05	2.582ValGln: 2.582 ± 0.054	2.793ValArg: 2.793 ± 0.054	4.596ValSer: 4.596 ± 0.064	4.155ValThr: 4.155 ± 0.067	5.054ValVal: 5.054 ± 0.085	0.641ValTrp: 0.641 ± 0.026	2.218ValTyr: 2.218 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.676TrpAla: 0.676 ± 0.025	0.088TrpCys: 0.088 ± 0.01	0.453TrpAsp: 0.453 ± 0.021	0.65TrpGlu: 0.65 ± 0.025	0.455TrpPhe: 0.455 ± 0.021	0.688TrpGly: 0.688 ± 0.03	0.211TrpHis: 0.211 ± 0.014	0.768TrpIle: 0.768 ± 0.029	0.782TrpLys: 0.782 ± 0.03	1.212TrpLeu: 1.212 ± 0.035	0.363TrpMet: 0.363 ± 0.016	0.473TrpAsn: 0.473 ± 0.023	0.307TrpPro: 0.307 ± 0.016	0.4TrpGln: 0.4 ± 0.022	0.395TrpArg: 0.395 ± 0.015	0.54TrpSer: 0.54 ± 0.023	0.54TrpThr: 0.54 ± 0.025	0.592TrpVal: 0.592 ± 0.022	0.118TrpTrp: 0.118 ± 0.009	0.283TrpTyr: 0.283 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.165TyrAla: 2.165 ± 0.043	0.311TyrCys: 0.311 ± 0.015	1.711TyrAsp: 1.711 ± 0.039	2.493TyrGlu: 2.493 ± 0.048	1.658TyrPhe: 1.658 ± 0.039	2.422TyrGly: 2.422 ± 0.049	0.747TyrHis: 0.747 ± 0.027	2.176TyrIle: 2.176 ± 0.048	1.938TyrLys: 1.938 ± 0.04	3.191TyrLeu: 3.191 ± 0.061	0.818TyrMet: 0.818 ± 0.028	1.272TyrAsn: 1.272 ± 0.032	1.33TyrPro: 1.33 ± 0.036	1.195TyrGln: 1.195 ± 0.034	1.507TyrArg: 1.507 ± 0.037	1.995TyrSer: 1.995 ± 0.049	1.733TyrThr: 1.733 ± 0.042	2.09TyrVal: 2.09 ± 0.044	0.35TyrTrp: 0.35 ± 0.019	1.224TyrTyr: 1.224 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3659 proteins (1091206 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---