Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Acidovorax; Acidovorax citrulli

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1TUF9|A1TUF9_ACIAC Endolytic peptidoglycan transglycosylase RlpA OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=rlpA PE=3 SV=1
MM1 pKa = 7.25SAVAEE6 pKa = 4.41NIQTEE11 pKa = 4.49MPAPIVFTDD20 pKa = 3.68SAAAKK25 pKa = 10.02VADD28 pKa = 5.45LIAEE32 pKa = 4.31EE33 pKa = 4.82GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.81EE60 pKa = 4.64ITNEE64 pKa = 4.77DD65 pKa = 4.2DD66 pKa = 3.25TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.32QYY85 pKa = 11.5LVGAEE90 pKa = 3.79IDD92 pKa = 3.91YY93 pKa = 11.36KK94 pKa = 11.19EE95 pKa = 4.67DD96 pKa = 3.23LQGAQFVIKK105 pKa = 10.4NPNATTTCGCGSSFSVV121 pKa = 3.54

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1TPI5|A1TPI5_ACIAC Uncharacterized protein OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=Aave_2295 PE=4 SV=1
MM1 pKa = 7.56GPGPLRR7 pKa = 11.84SRR9 pKa = 11.84AQRR12 pKa = 11.84QARR15 pKa = 11.84HH16 pKa = 5.85AARR19 pKa = 11.84HH20 pKa = 4.71LQRR23 pKa = 11.84PGPHH27 pKa = 6.64AFRR30 pKa = 11.84RR31 pKa = 11.84SARR34 pKa = 11.84GWFRR38 pKa = 11.84LHH40 pKa = 6.98RR41 pKa = 11.84PRR43 pKa = 11.84PHH45 pKa = 7.12GGRR48 pKa = 11.84QRR50 pKa = 11.84GAGGQHH56 pKa = 6.39PAARR60 pKa = 11.84GHH62 pKa = 6.66PWTGGRR68 pKa = 11.84AGRR71 pKa = 11.84PGGVRR76 pKa = 11.84GRR78 pKa = 11.84QGRR81 pKa = 11.84EE82 pKa = 3.7GEE84 pKa = 3.88AGGWRR89 pKa = 11.84RR90 pKa = 11.84HH91 pKa = 5.24GLQEE95 pKa = 3.89FNRR98 pKa = 11.84WRR100 pKa = 11.84AAPVRR105 pKa = 11.84VGPPVV110 pKa = 2.97

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4602

0

4602

1557338

37

3401

338.4

36.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.853 ± 0.046

0.95 ± 0.012

5.193 ± 0.023

5.242 ± 0.032

3.258 ± 0.024

8.787 ± 0.036

2.357 ± 0.019

3.891 ± 0.023

2.672 ± 0.034

10.341 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.015

2.242 ± 0.02

5.814 ± 0.033

4.079 ± 0.024

7.681 ± 0.034

5.197 ± 0.027

5.011 ± 0.028

7.433 ± 0.028

1.526 ± 0.015

2.113 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski