Amino acid dipepetide frequency for Synechococcus phage metaG-MbCM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.122AlaAla: 6.122 ± 0.534
0.513AlaCys: 0.513 ± 0.112
4.221AlaAsp: 4.221 ± 0.346
3.746AlaGlu: 3.746 ± 0.394
2.681AlaPhe: 2.681 ± 0.187
6.503AlaGly: 6.503 ± 0.427
0.799AlaHis: 0.799 ± 0.127
4.278AlaIle: 4.278 ± 0.285
4.107AlaLys: 4.107 ± 0.39
4.392AlaLeu: 4.392 ± 0.303
1.578AlaMet: 1.578 ± 0.205
3.803AlaAsn: 3.803 ± 0.315
2.358AlaPro: 2.358 ± 0.191
2.434AlaGln: 2.434 ± 0.215
2.833AlaArg: 2.833 ± 0.274
5.514AlaSer: 5.514 ± 0.379
6.331AlaThr: 6.331 ± 0.476
4.601AlaVal: 4.601 ± 0.32
0.665AlaTrp: 0.665 ± 0.108
2.567AlaTyr: 2.567 ± 0.213
0.0AlaXaa: 0.0 ± 0.0
Cys
0.742CysAla: 0.742 ± 0.124
0.19CysCys: 0.19 ± 0.074
0.513CysAsp: 0.513 ± 0.106
0.608CysGlu: 0.608 ± 0.126
0.475CysPhe: 0.475 ± 0.099
0.361CysGly: 0.361 ± 0.076
0.228CysHis: 0.228 ± 0.065
0.399CysIle: 0.399 ± 0.098
0.551CysLys: 0.551 ± 0.096
0.799CysLeu: 0.799 ± 0.13
0.323CysMet: 0.323 ± 0.096
0.513CysAsn: 0.513 ± 0.132
0.342CysPro: 0.342 ± 0.091
0.437CysGln: 0.437 ± 0.095
0.38CysArg: 0.38 ± 0.096
0.723CysSer: 0.723 ± 0.105
0.703CysThr: 0.703 ± 0.119
0.418CysVal: 0.418 ± 0.09
0.114CysTrp: 0.114 ± 0.059
0.304CysTyr: 0.304 ± 0.072
0.0CysXaa: 0.0 ± 0.0
Asp
4.677AspAla: 4.677 ± 0.287
0.646AspCys: 0.646 ± 0.125
4.544AspAsp: 4.544 ± 0.331
3.441AspGlu: 3.441 ± 0.32
2.795AspPhe: 2.795 ± 0.229
5.913AspGly: 5.913 ± 0.354
0.989AspHis: 0.989 ± 0.151
3.689AspIle: 3.689 ± 0.278
3.042AspLys: 3.042 ± 0.266
4.886AspLeu: 4.886 ± 0.301
1.635AspMet: 1.635 ± 0.206
3.822AspAsn: 3.822 ± 0.343
3.194AspPro: 3.194 ± 0.287
2.263AspGln: 2.263 ± 0.225
2.548AspArg: 2.548 ± 0.23
4.164AspSer: 4.164 ± 0.286
4.753AspThr: 4.753 ± 0.36
4.316AspVal: 4.316 ± 0.246
0.856AspTrp: 0.856 ± 0.142
3.308AspTyr: 3.308 ± 0.271
0.0AspXaa: 0.0 ± 0.0
Glu
3.384GluAla: 3.384 ± 0.276
0.665GluCys: 0.665 ± 0.143
3.651GluAsp: 3.651 ± 0.306
4.601GluGlu: 4.601 ± 0.54
2.833GluPhe: 2.833 ± 0.248
3.593GluGly: 3.593 ± 0.311
0.951GluHis: 0.951 ± 0.16
3.765GluIle: 3.765 ± 0.327
3.593GluLys: 3.593 ± 0.444
4.677GluLeu: 4.677 ± 0.351
1.635GluMet: 1.635 ± 0.215
2.7GluAsn: 2.7 ± 0.257
1.616GluPro: 1.616 ± 0.185
2.225GluGln: 2.225 ± 0.212
2.225GluArg: 2.225 ± 0.226
3.403GluSer: 3.403 ± 0.302
3.727GluThr: 3.727 ± 0.276
4.468GluVal: 4.468 ± 0.331
0.799GluTrp: 0.799 ± 0.138
2.757GluTyr: 2.757 ± 0.277
0.0GluXaa: 0.0 ± 0.0
Phe
2.491PheAla: 2.491 ± 0.238
0.285PheCys: 0.285 ± 0.081
3.86PheAsp: 3.86 ± 0.247
2.738PheGlu: 2.738 ± 0.212
1.749PhePhe: 1.749 ± 0.179
3.004PheGly: 3.004 ± 0.234
0.684PheHis: 0.684 ± 0.105
2.339PheIle: 2.339 ± 0.234
2.491PheLys: 2.491 ± 0.242
2.89PheLeu: 2.89 ± 0.23
1.141PheMet: 1.141 ± 0.155
2.757PheAsn: 2.757 ± 0.214
1.692PhePro: 1.692 ± 0.202
1.578PheGln: 1.578 ± 0.17
1.502PheArg: 1.502 ± 0.161
3.289PheSer: 3.289 ± 0.245
3.822PheThr: 3.822 ± 0.34
2.681PheVal: 2.681 ± 0.247
0.38PheTrp: 0.38 ± 0.073
1.597PheTyr: 1.597 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
5.856GlyAla: 5.856 ± 0.427
0.799GlyCys: 0.799 ± 0.15
4.943GlyAsp: 4.943 ± 0.345
3.803GlyGlu: 3.803 ± 0.26
3.194GlyPhe: 3.194 ± 0.256
7.776GlyGly: 7.776 ± 0.612
1.236GlyHis: 1.236 ± 0.179
4.563GlyIle: 4.563 ± 0.237
3.879GlyLys: 3.879 ± 0.34
4.696GlyLeu: 4.696 ± 0.31
1.654GlyMet: 1.654 ± 0.214
4.487GlyAsn: 4.487 ± 0.354
2.034GlyPro: 2.034 ± 0.206
2.871GlyGln: 2.871 ± 0.265
2.662GlyArg: 2.662 ± 0.237
7.377GlySer: 7.377 ± 0.53
7.51GlyThr: 7.51 ± 0.69
4.924GlyVal: 4.924 ± 0.32
0.951GlyTrp: 0.951 ± 0.141
3.574GlyTyr: 3.574 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
0.646HisAla: 0.646 ± 0.134
0.247HisCys: 0.247 ± 0.066
1.027HisAsp: 1.027 ± 0.162
0.875HisGlu: 0.875 ± 0.137
0.723HisPhe: 0.723 ± 0.132
0.97HisGly: 0.97 ± 0.128
0.342HisHis: 0.342 ± 0.097
1.008HisIle: 1.008 ± 0.157
0.97HisLys: 0.97 ± 0.151
1.084HisLeu: 1.084 ± 0.137
0.399HisMet: 0.399 ± 0.084
0.723HisAsn: 0.723 ± 0.135
0.856HisPro: 0.856 ± 0.155
0.646HisGln: 0.646 ± 0.127
0.627HisArg: 0.627 ± 0.122
1.008HisSer: 1.008 ± 0.137
1.16HisThr: 1.16 ± 0.17
0.97HisVal: 0.97 ± 0.142
0.228HisTrp: 0.228 ± 0.068
0.875HisTyr: 0.875 ± 0.137
0.0HisXaa: 0.0 ± 0.0
Ile
4.031IleAla: 4.031 ± 0.298
0.57IleCys: 0.57 ± 0.092
4.563IleAsp: 4.563 ± 0.313
3.498IleGlu: 3.498 ± 0.244
2.51IlePhe: 2.51 ± 0.25
3.955IleGly: 3.955 ± 0.305
0.761IleHis: 0.761 ± 0.118
3.27IleIle: 3.27 ± 0.266
3.708IleLys: 3.708 ± 0.305
3.879IleLeu: 3.879 ± 0.27
1.027IleMet: 1.027 ± 0.152
4.221IleAsn: 4.221 ± 0.331
2.852IlePro: 2.852 ± 0.256
2.624IleGln: 2.624 ± 0.245
2.015IleArg: 2.015 ± 0.171
4.715IleSer: 4.715 ± 0.327
5.59IleThr: 5.59 ± 0.613
3.727IleVal: 3.727 ± 0.287
0.703IleTrp: 0.703 ± 0.111
1.73IleTyr: 1.73 ± 0.209
0.0IleXaa: 0.0 ± 0.0
Lys
3.555LysAla: 3.555 ± 0.37
0.608LysCys: 0.608 ± 0.113
3.365LysAsp: 3.365 ± 0.273
3.841LysGlu: 3.841 ± 0.461
2.51LysPhe: 2.51 ± 0.226
3.555LysGly: 3.555 ± 0.41
0.799LysHis: 0.799 ± 0.135
4.145LysIle: 4.145 ± 0.365
4.563LysLys: 4.563 ± 0.527
4.582LysLeu: 4.582 ± 0.367
1.54LysMet: 1.54 ± 0.232
2.7LysAsn: 2.7 ± 0.262
1.863LysPro: 1.863 ± 0.23
2.339LysGln: 2.339 ± 0.29
2.529LysArg: 2.529 ± 0.269
3.822LysSer: 3.822 ± 0.314
3.803LysThr: 3.803 ± 0.283
4.411LysVal: 4.411 ± 0.302
0.761LysTrp: 0.761 ± 0.127
2.757LysTyr: 2.757 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
4.62LeuAla: 4.62 ± 0.329
0.742LeuCys: 0.742 ± 0.139
5.343LeuAsp: 5.343 ± 0.331
4.259LeuGlu: 4.259 ± 0.327
2.795LeuPhe: 2.795 ± 0.275
4.506LeuGly: 4.506 ± 0.301
1.16LeuHis: 1.16 ± 0.161
3.403LeuIle: 3.403 ± 0.249
4.715LeuLys: 4.715 ± 0.428
5.0LeuLeu: 5.0 ± 0.343
1.578LeuMet: 1.578 ± 0.193
4.753LeuAsn: 4.753 ± 0.292
3.365LeuPro: 3.365 ± 0.266
2.814LeuGln: 2.814 ± 0.22
3.289LeuArg: 3.289 ± 0.257
5.096LeuSer: 5.096 ± 0.308
5.78LeuThr: 5.78 ± 0.384
4.449LeuVal: 4.449 ± 0.237
0.589LeuTrp: 0.589 ± 0.116
3.46LeuTyr: 3.46 ± 0.286
0.0LeuXaa: 0.0 ± 0.0
Met
1.597MetAla: 1.597 ± 0.22
0.209MetCys: 0.209 ± 0.07
1.293MetAsp: 1.293 ± 0.19
1.141MetGlu: 1.141 ± 0.142
0.875MetPhe: 0.875 ± 0.147
1.312MetGly: 1.312 ± 0.205
0.532MetHis: 0.532 ± 0.115
1.312MetIle: 1.312 ± 0.179
1.806MetLys: 1.806 ± 0.259
1.768MetLeu: 1.768 ± 0.196
0.494MetMet: 0.494 ± 0.103
1.445MetAsn: 1.445 ± 0.163
0.646MetPro: 0.646 ± 0.111
0.78MetGln: 0.78 ± 0.135
1.179MetArg: 1.179 ± 0.161
1.844MetSer: 1.844 ± 0.226
1.597MetThr: 1.597 ± 0.184
1.217MetVal: 1.217 ± 0.144
0.247MetTrp: 0.247 ± 0.08
0.761MetTyr: 0.761 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
4.107AsnAla: 4.107 ± 0.293
0.475AsnCys: 0.475 ± 0.085
3.061AsnAsp: 3.061 ± 0.226
2.833AsnGlu: 2.833 ± 0.226
2.662AsnPhe: 2.662 ± 0.203
4.715AsnGly: 4.715 ± 0.466
1.046AsnHis: 1.046 ± 0.156
3.689AsnIle: 3.689 ± 0.308
2.871AsnLys: 2.871 ± 0.291
4.791AsnLeu: 4.791 ± 0.358
0.894AsnMet: 0.894 ± 0.15
3.498AsnAsn: 3.498 ± 0.282
3.194AsnPro: 3.194 ± 0.306
2.091AsnGln: 2.091 ± 0.191
2.434AsnArg: 2.434 ± 0.206
3.955AsnSer: 3.955 ± 0.273
4.354AsnThr: 4.354 ± 0.359
4.715AsnVal: 4.715 ± 0.327
0.665AsnTrp: 0.665 ± 0.14
2.586AsnTyr: 2.586 ± 0.245
0.0AsnXaa: 0.0 ± 0.0
Pro
2.244ProAla: 2.244 ± 0.22
0.285ProCys: 0.285 ± 0.078
2.434ProAsp: 2.434 ± 0.225
2.605ProGlu: 2.605 ± 0.265
1.806ProPhe: 1.806 ± 0.185
3.156ProGly: 3.156 ± 0.26
0.665ProHis: 0.665 ± 0.102
2.225ProIle: 2.225 ± 0.213
1.92ProLys: 1.92 ± 0.26
2.358ProLeu: 2.358 ± 0.197
0.761ProMet: 0.761 ± 0.164
2.415ProAsn: 2.415 ± 0.223
1.844ProPro: 1.844 ± 0.26
1.236ProGln: 1.236 ± 0.184
1.673ProArg: 1.673 ± 0.224
3.175ProSer: 3.175 ± 0.232
3.004ProThr: 3.004 ± 0.261
2.719ProVal: 2.719 ± 0.211
0.551ProTrp: 0.551 ± 0.096
1.54ProTyr: 1.54 ± 0.161
0.0ProXaa: 0.0 ± 0.0
Gln
2.491GlnAla: 2.491 ± 0.222
0.285GlnCys: 0.285 ± 0.068
1.901GlnAsp: 1.901 ± 0.195
2.586GlnGlu: 2.586 ± 0.257
1.654GlnPhe: 1.654 ± 0.189
2.681GlnGly: 2.681 ± 0.204
0.646GlnHis: 0.646 ± 0.11
2.814GlnIle: 2.814 ± 0.265
2.434GlnLys: 2.434 ± 0.262
3.137GlnLeu: 3.137 ± 0.232
0.97GlnMet: 0.97 ± 0.162
2.32GlnAsn: 2.32 ± 0.223
1.274GlnPro: 1.274 ± 0.141
1.464GlnGln: 1.464 ± 0.176
1.597GlnArg: 1.597 ± 0.147
2.263GlnSer: 2.263 ± 0.221
2.529GlnThr: 2.529 ± 0.222
2.757GlnVal: 2.757 ± 0.209
0.38GlnTrp: 0.38 ± 0.09
1.749GlnTyr: 1.749 ± 0.168
0.0GlnXaa: 0.0 ± 0.0
Arg
2.529ArgAla: 2.529 ± 0.231
0.361ArgCys: 0.361 ± 0.079
2.358ArgAsp: 2.358 ± 0.218
2.301ArgGlu: 2.301 ± 0.263
1.977ArgPhe: 1.977 ± 0.19
2.985ArgGly: 2.985 ± 0.277
0.57ArgHis: 0.57 ± 0.107
2.852ArgIle: 2.852 ± 0.262
2.301ArgLys: 2.301 ± 0.243
3.289ArgLeu: 3.289 ± 0.285
1.046ArgMet: 1.046 ± 0.157
2.034ArgAsn: 2.034 ± 0.184
1.293ArgPro: 1.293 ± 0.182
1.388ArgGln: 1.388 ± 0.159
1.882ArgArg: 1.882 ± 0.236
2.377ArgSer: 2.377 ± 0.223
2.225ArgThr: 2.225 ± 0.256
2.947ArgVal: 2.947 ± 0.242
0.342ArgTrp: 0.342 ± 0.084
1.939ArgTyr: 1.939 ± 0.215
0.0ArgXaa: 0.0 ± 0.0
Ser
5.932SerAla: 5.932 ± 0.437
0.361SerCys: 0.361 ± 0.089
4.373SerAsp: 4.373 ± 0.369
3.479SerGlu: 3.479 ± 0.255
3.46SerPhe: 3.46 ± 0.244
7.738SerGly: 7.738 ± 0.547
1.065SerHis: 1.065 ± 0.16
4.81SerIle: 4.81 ± 0.422
3.555SerLys: 3.555 ± 0.282
4.905SerLeu: 4.905 ± 0.247
1.654SerMet: 1.654 ± 0.222
4.145SerAsn: 4.145 ± 0.354
2.529SerPro: 2.529 ± 0.239
2.662SerGln: 2.662 ± 0.219
2.11SerArg: 2.11 ± 0.211
6.103SerSer: 6.103 ± 0.56
5.951SerThr: 5.951 ± 0.535
5.019SerVal: 5.019 ± 0.347
0.532SerTrp: 0.532 ± 0.099
2.738SerTyr: 2.738 ± 0.226
0.0SerXaa: 0.0 ± 0.0
Thr
6.769ThrAla: 6.769 ± 0.546
0.589ThrCys: 0.589 ± 0.101
4.24ThrAsp: 4.24 ± 0.353
3.955ThrGlu: 3.955 ± 0.298
3.765ThrPhe: 3.765 ± 0.341
7.377ThrGly: 7.377 ± 0.649
1.008ThrHis: 1.008 ± 0.122
4.734ThrIle: 4.734 ± 0.426
3.841ThrLys: 3.841 ± 0.268
5.989ThrLeu: 5.989 ± 0.502
1.141ThrMet: 1.141 ± 0.161
4.506ThrAsn: 4.506 ± 0.426
3.137ThrPro: 3.137 ± 0.279
3.27ThrGln: 3.27 ± 0.248
2.586ThrArg: 2.586 ± 0.277
5.856ThrSer: 5.856 ± 0.581
6.978ThrThr: 6.978 ± 0.677
6.008ThrVal: 6.008 ± 0.513
0.799ThrTrp: 0.799 ± 0.123
3.213ThrTyr: 3.213 ± 0.247
0.0ThrXaa: 0.0 ± 0.0
Val
4.962ValAla: 4.962 ± 0.325
0.513ValCys: 0.513 ± 0.091
5.343ValAsp: 5.343 ± 0.411
4.278ValGlu: 4.278 ± 0.291
2.51ValPhe: 2.51 ± 0.252
5.343ValGly: 5.343 ± 0.315
0.837ValHis: 0.837 ± 0.149
3.555ValIle: 3.555 ± 0.271
4.24ValLys: 4.24 ± 0.373
4.525ValLeu: 4.525 ± 0.335
1.331ValMet: 1.331 ± 0.18
4.202ValAsn: 4.202 ± 0.307
2.776ValPro: 2.776 ± 0.203
2.643ValGln: 2.643 ± 0.201
2.567ValArg: 2.567 ± 0.185
5.267ValSer: 5.267 ± 0.379
6.027ValThr: 6.027 ± 0.517
4.772ValVal: 4.772 ± 0.408
0.646ValTrp: 0.646 ± 0.123
2.548ValTyr: 2.548 ± 0.226
0.0ValXaa: 0.0 ± 0.0
Trp
0.723TrpAla: 0.723 ± 0.13
0.114TrpCys: 0.114 ± 0.056
0.703TrpAsp: 0.703 ± 0.14
0.494TrpGlu: 0.494 ± 0.1
0.513TrpPhe: 0.513 ± 0.12
0.875TrpGly: 0.875 ± 0.145
0.323TrpHis: 0.323 ± 0.089
0.513TrpIle: 0.513 ± 0.094
0.875TrpLys: 0.875 ± 0.143
0.742TrpLeu: 0.742 ± 0.116
0.304TrpMet: 0.304 ± 0.073
0.837TrpAsn: 0.837 ± 0.133
0.114TrpPro: 0.114 ± 0.041
0.418TrpGln: 0.418 ± 0.099
0.247TrpArg: 0.247 ± 0.072
0.799TrpSer: 0.799 ± 0.128
0.78TrpThr: 0.78 ± 0.121
0.761TrpVal: 0.761 ± 0.155
0.133TrpTrp: 0.133 ± 0.057
0.513TrpTyr: 0.513 ± 0.095
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.567TyrAla: 2.567 ± 0.227
0.665TyrCys: 0.665 ± 0.12
3.746TyrAsp: 3.746 ± 0.256
2.187TyrGlu: 2.187 ± 0.229
1.483TyrPhe: 1.483 ± 0.143
2.472TyrGly: 2.472 ± 0.211
0.742TyrHis: 0.742 ± 0.148
2.586TyrIle: 2.586 ± 0.22
2.586TyrLys: 2.586 ± 0.288
3.327TyrLeu: 3.327 ± 0.289
0.932TyrMet: 0.932 ± 0.144
2.757TyrAsn: 2.757 ± 0.234
1.635TyrPro: 1.635 ± 0.187
1.749TyrGln: 1.749 ± 0.199
2.11TyrArg: 2.11 ± 0.237
2.377TyrSer: 2.377 ± 0.197
3.137TyrThr: 3.137 ± 0.3
3.004TyrVal: 3.004 ± 0.21
0.437TyrTrp: 0.437 ± 0.116
1.939TyrTyr: 1.939 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 234 proteins (52596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski