Amino acid dipepetide frequency for Clostridium gasigenes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.433AlaAla: 3.433 ± 0.077	0.674AlaCys: 0.674 ± 0.025	2.487AlaAsp: 2.487 ± 0.053	3.289AlaGlu: 3.289 ± 0.06	2.405AlaPhe: 2.405 ± 0.045	3.719AlaGly: 3.719 ± 0.071	0.773AlaHis: 0.773 ± 0.029	5.997AlaIle: 5.997 ± 0.076	4.668AlaLys: 4.668 ± 0.07	5.655AlaLeu: 5.655 ± 0.079	1.642AlaMet: 1.642 ± 0.041	2.693AlaAsn: 2.693 ± 0.05	1.351AlaPro: 1.351 ± 0.039	1.327AlaGln: 1.327 ± 0.038	1.715AlaArg: 1.715 ± 0.044	3.314AlaSer: 3.314 ± 0.062	3.085AlaThr: 3.085 ± 0.071	3.631AlaVal: 3.631 ± 0.063	0.302AlaTrp: 0.302 ± 0.016	2.015AlaTyr: 2.015 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.545CysAla: 0.545 ± 0.025	0.202CysCys: 0.202 ± 0.019	0.662CysAsp: 0.662 ± 0.026	0.849CysGlu: 0.849 ± 0.029	0.475CysPhe: 0.475 ± 0.022	1.106CysGly: 1.106 ± 0.038	0.203CysHis: 0.203 ± 0.015	1.205CysIle: 1.205 ± 0.029	0.98CysLys: 0.98 ± 0.03	0.827CysLeu: 0.827 ± 0.028	0.271CysMet: 0.271 ± 0.016	0.789CysAsn: 0.789 ± 0.027	0.427CysPro: 0.427 ± 0.024	0.201CysGln: 0.201 ± 0.014	0.358CysArg: 0.358 ± 0.021	0.747CysSer: 0.747 ± 0.025	0.602CysThr: 0.602 ± 0.025	0.647CysVal: 0.647 ± 0.024	0.057CysTrp: 0.057 ± 0.007	0.462CysTyr: 0.462 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	2.546AspAla: 2.546 ± 0.051	0.598AspCys: 0.598 ± 0.025	2.57AspAsp: 2.57 ± 0.048	4.619AspGlu: 4.619 ± 0.062	2.709AspPhe: 2.709 ± 0.049	3.542AspGly: 3.542 ± 0.064	0.545AspHis: 0.545 ± 0.022	6.26AspIle: 6.26 ± 0.082	5.45AspLys: 5.45 ± 0.082	4.956AspLeu: 4.956 ± 0.076	1.453AspMet: 1.453 ± 0.038	3.427AspAsn: 3.427 ± 0.056	1.217AspPro: 1.217 ± 0.038	0.698AspGln: 0.698 ± 0.027	1.753AspArg: 1.753 ± 0.044	3.094AspSer: 3.094 ± 0.059	2.554AspThr: 2.554 ± 0.051	3.52AspVal: 3.52 ± 0.058	0.336AspTrp: 0.336 ± 0.019	2.579AspTyr: 2.579 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	4.117GluAla: 4.117 ± 0.071	0.745GluCys: 0.745 ± 0.028	4.443GluAsp: 4.443 ± 0.07	7.313GluGlu: 7.313 ± 0.1	3.187GluPhe: 3.187 ± 0.055	4.238GluGly: 4.238 ± 0.075	0.811GluHis: 0.811 ± 0.025	7.729GluIle: 7.729 ± 0.096	7.691GluLys: 7.691 ± 0.103	6.998GluLeu: 6.998 ± 0.086	2.014GluMet: 2.014 ± 0.044	5.539GluAsn: 5.539 ± 0.074	1.323GluPro: 1.323 ± 0.036	1.611GluGln: 1.611 ± 0.043	2.409GluArg: 2.409 ± 0.049	3.868GluSer: 3.868 ± 0.06	3.142GluThr: 3.142 ± 0.058	5.469GluVal: 5.469 ± 0.082	0.481GluTrp: 0.481 ± 0.021	3.281GluTyr: 3.281 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	2.248PheAla: 2.248 ± 0.044	0.457PheCys: 0.457 ± 0.018	2.406PheAsp: 2.406 ± 0.047	2.774PheGlu: 2.774 ± 0.051	1.82PhePhe: 1.82 ± 0.046	2.893PheGly: 2.893 ± 0.058	0.51PheHis: 0.51 ± 0.022	4.902PheIle: 4.902 ± 0.087	4.078PheLys: 4.078 ± 0.07	3.708PheLeu: 3.708 ± 0.068	1.205PheMet: 1.205 ± 0.028	3.145PheAsn: 3.145 ± 0.06	1.031PhePro: 1.031 ± 0.03	0.852PheGln: 0.852 ± 0.028	1.218PheArg: 1.218 ± 0.037	3.002PheSer: 3.002 ± 0.057	2.33PheThr: 2.33 ± 0.048	2.474PheVal: 2.474 ± 0.042	0.317PheTrp: 0.317 ± 0.019	1.693PheTyr: 1.693 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	3.987GlyAla: 3.987 ± 0.09	0.9GlyCys: 0.9 ± 0.03	3.382GlyAsp: 3.382 ± 0.065	4.666GlyGlu: 4.666 ± 0.063	3.133GlyPhe: 3.133 ± 0.06	4.383GlyGly: 4.383 ± 0.088	0.914GlyHis: 0.914 ± 0.03	6.984GlyIle: 6.984 ± 0.091	5.531GlyLys: 5.531 ± 0.072	5.781GlyLeu: 5.781 ± 0.085	1.823GlyMet: 1.823 ± 0.064	3.456GlyAsn: 3.456 ± 0.074	1.276GlyPro: 1.276 ± 0.045	1.485GlyGln: 1.485 ± 0.04	2.177GlyArg: 2.177 ± 0.047	3.745GlySer: 3.745 ± 0.064	3.574GlyThr: 3.574 ± 0.07	4.832GlyVal: 4.832 ± 0.072	0.471GlyTrp: 0.471 ± 0.021	3.003GlyTyr: 3.003 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	0.566HisAla: 0.566 ± 0.024	0.212HisCys: 0.212 ± 0.014	0.573HisAsp: 0.573 ± 0.025	0.774HisGlu: 0.774 ± 0.026	0.562HisPhe: 0.562 ± 0.023	0.942HisGly: 0.942 ± 0.031	0.273HisHis: 0.273 ± 0.017	1.215HisIle: 1.215 ± 0.034	1.052HisLys: 1.052 ± 0.032	1.088HisLeu: 1.088 ± 0.031	0.341HisMet: 0.341 ± 0.019	0.788HisAsn: 0.788 ± 0.03	0.524HisPro: 0.524 ± 0.023	0.267HisGln: 0.267 ± 0.015	0.482HisArg: 0.482 ± 0.022	0.823HisSer: 0.823 ± 0.027	0.656HisThr: 0.656 ± 0.025	0.679HisVal: 0.679 ± 0.024	0.091HisTrp: 0.091 ± 0.008	0.509HisTyr: 0.509 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.823IleAla: 5.823 ± 0.086	1.235IleCys: 1.235 ± 0.038	6.056IleAsp: 6.056 ± 0.078	7.719IleGlu: 7.719 ± 0.099	4.329IlePhe: 4.329 ± 0.085	6.538IleGly: 6.538 ± 0.088	1.157IleHis: 1.157 ± 0.033	10.558IleIle: 10.558 ± 0.141	9.43IleLys: 9.43 ± 0.111	9.196IleLeu: 9.196 ± 0.112	2.492IleMet: 2.492 ± 0.046	6.963IleAsn: 6.963 ± 0.09	3.194IlePro: 3.194 ± 0.053	2.184IleGln: 2.184 ± 0.041	3.073IleArg: 3.073 ± 0.048	7.189IleSer: 7.189 ± 0.098	5.392IleThr: 5.392 ± 0.077	6.56IleVal: 6.56 ± 0.09	0.594IleTrp: 0.594 ± 0.023	3.798IleTyr: 3.798 ± 0.066	0.0IleXaa: 0.0 ± 0.0
Lys	4.805LysAla: 4.805 ± 0.07	0.913LysCys: 0.913 ± 0.026	5.94LysAsp: 5.94 ± 0.086	9.162LysGlu: 9.162 ± 0.105	3.274LysPhe: 3.274 ± 0.051	5.337LysGly: 5.337 ± 0.081	1.019LysHis: 1.019 ± 0.033	8.819LysIle: 8.819 ± 0.096	8.337LysLys: 8.337 ± 0.107	7.781LysLeu: 7.781 ± 0.083	2.389LysMet: 2.389 ± 0.047	6.429LysAsn: 6.429 ± 0.092	2.03LysPro: 2.03 ± 0.043	2.004LysGln: 2.004 ± 0.048	2.918LysArg: 2.918 ± 0.057	5.538LysSer: 5.538 ± 0.081	4.198LysThr: 4.198 ± 0.055	6.204LysVal: 6.204 ± 0.089	0.607LysTrp: 0.607 ± 0.024	4.269LysTyr: 4.269 ± 0.07	0.001LysXaa: 0.001 ± 0.001
Leu	5.135LeuAla: 5.135 ± 0.08	1.045LeuCys: 1.045 ± 0.028	5.163LeuAsp: 5.163 ± 0.074	6.723LeuGlu: 6.723 ± 0.096	3.534LeuPhe: 3.534 ± 0.071	6.096LeuGly: 6.096 ± 0.08	1.057LeuHis: 1.057 ± 0.035	8.76LeuIle: 8.76 ± 0.095	8.53LeuLys: 8.53 ± 0.103	7.854LeuLeu: 7.854 ± 0.106	2.335LeuMet: 2.335 ± 0.046	5.926LeuAsn: 5.926 ± 0.072	2.533LeuPro: 2.533 ± 0.052	1.983LeuGln: 1.983 ± 0.042	3.012LeuArg: 3.012 ± 0.056	6.474LeuSer: 6.474 ± 0.084	4.6LeuThr: 4.6 ± 0.065	5.713LeuVal: 5.713 ± 0.07	0.608LeuTrp: 0.608 ± 0.024	3.163LeuTyr: 3.163 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	1.694MetAla: 1.694 ± 0.047	0.267MetCys: 0.267 ± 0.017	1.515MetAsp: 1.515 ± 0.036	1.915MetGlu: 1.915 ± 0.044	1.108MetPhe: 1.108 ± 0.033	1.906MetGly: 1.906 ± 0.046	0.315MetHis: 0.315 ± 0.016	2.422MetIle: 2.422 ± 0.046	2.575MetLys: 2.575 ± 0.05	2.24MetLeu: 2.24 ± 0.05	0.707MetMet: 0.707 ± 0.024	1.658MetAsn: 1.658 ± 0.039	0.921MetPro: 0.921 ± 0.028	0.714MetGln: 0.714 ± 0.043	0.85MetArg: 0.85 ± 0.026	1.633MetSer: 1.633 ± 0.036	1.162MetThr: 1.162 ± 0.036	1.653MetVal: 1.653 ± 0.046	0.171MetTrp: 0.171 ± 0.012	0.899MetTyr: 0.899 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.736AsnAla: 2.736 ± 0.053	0.744AsnCys: 0.744 ± 0.034	3.005AsnAsp: 3.005 ± 0.046	4.793AsnGlu: 4.793 ± 0.079	2.559AsnPhe: 2.559 ± 0.059	3.951AsnGly: 3.951 ± 0.063	0.742AsnHis: 0.742 ± 0.028	7.605AsnIle: 7.605 ± 0.096	6.842AsnLys: 6.842 ± 0.092	5.739AsnLeu: 5.739 ± 0.076	1.762AsnMet: 1.762 ± 0.041	4.926AsnAsn: 4.926 ± 0.093	2.066AsnPro: 2.066 ± 0.043	1.357AsnGln: 1.357 ± 0.035	2.042AsnArg: 2.042 ± 0.047	4.08AsnSer: 4.08 ± 0.066	3.116AsnThr: 3.116 ± 0.053	3.507AsnVal: 3.507 ± 0.062	0.446AsnTrp: 0.446 ± 0.023	2.698AsnTyr: 2.698 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	1.326ProAla: 1.326 ± 0.038	0.287ProCys: 0.287 ± 0.016	1.233ProAsp: 1.233 ± 0.04	1.974ProGlu: 1.974 ± 0.045	1.332ProPhe: 1.332 ± 0.036	1.617ProGly: 1.617 ± 0.036	0.438ProHis: 0.438 ± 0.022	2.735ProIle: 2.735 ± 0.047	2.156ProLys: 2.156 ± 0.045	2.471ProLeu: 2.471 ± 0.05	0.74ProMet: 0.74 ± 0.027	1.566ProAsn: 1.566 ± 0.04	0.49ProPro: 0.49 ± 0.023	0.707ProGln: 0.707 ± 0.029	0.759ProArg: 0.759 ± 0.032	1.686ProSer: 1.686 ± 0.034	1.451ProThr: 1.451 ± 0.043	1.862ProVal: 1.862 ± 0.045	0.239ProTrp: 0.239 ± 0.017	1.151ProTyr: 1.151 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	1.249GlnAla: 1.249 ± 0.036	0.249GlnCys: 0.249 ± 0.015	1.065GlnAsp: 1.065 ± 0.031	1.603GlnGlu: 1.603 ± 0.04	0.891GlnPhe: 0.891 ± 0.028	1.626GlnGly: 1.626 ± 0.048	0.274GlnHis: 0.274 ± 0.017	2.004GlnIle: 2.004 ± 0.043	1.827GlnLys: 1.827 ± 0.045	1.882GlnLeu: 1.882 ± 0.041	0.595GlnMet: 0.595 ± 0.025	1.341GlnAsn: 1.341 ± 0.032	0.503GlnPro: 0.503 ± 0.021	0.55GlnGln: 0.55 ± 0.025	0.881GlnArg: 0.881 ± 0.03	1.358GlnSer: 1.358 ± 0.036	1.012GlnThr: 1.012 ± 0.03	1.452GlnVal: 1.452 ± 0.038	0.219GlnTrp: 0.219 ± 0.014	0.942GlnTyr: 0.942 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	1.748ArgAla: 1.748 ± 0.044	0.4ArgCys: 0.4 ± 0.018	1.913ArgAsp: 1.913 ± 0.042	2.868ArgGlu: 2.868 ± 0.059	1.441ArgPhe: 1.441 ± 0.037	2.136ArgGly: 2.136 ± 0.047	0.39ArgHis: 0.39 ± 0.021	3.042ArgIle: 3.042 ± 0.053	2.749ArgLys: 2.749 ± 0.051	2.756ArgLeu: 2.756 ± 0.051	0.851ArgMet: 0.851 ± 0.031	2.016ArgAsn: 2.016 ± 0.046	0.734ArgPro: 0.734 ± 0.029	0.742ArgGln: 0.742 ± 0.021	1.296ArgArg: 1.296 ± 0.039	1.629ArgSer: 1.629 ± 0.037	1.47ArgThr: 1.47 ± 0.04	2.249ArgVal: 2.249 ± 0.051	0.24ArgTrp: 0.24 ± 0.016	1.358ArgTyr: 1.358 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.054SerAla: 3.054 ± 0.054	0.699SerCys: 0.699 ± 0.026	3.061SerAsp: 3.061 ± 0.051	4.27SerGlu: 4.27 ± 0.067	2.993SerPhe: 2.993 ± 0.05	4.219SerGly: 4.219 ± 0.069	0.88SerHis: 0.88 ± 0.024	6.823SerIle: 6.823 ± 0.091	6.125SerLys: 6.125 ± 0.076	6.066SerLeu: 6.066 ± 0.083	1.683SerMet: 1.683 ± 0.041	3.891SerAsn: 3.891 ± 0.07	1.552SerPro: 1.552 ± 0.037	1.494SerGln: 1.494 ± 0.039	2.044SerArg: 2.044 ± 0.051	4.229SerSer: 4.229 ± 0.078	3.239SerThr: 3.239 ± 0.06	3.76SerVal: 3.76 ± 0.059	0.441SerTrp: 0.441 ± 0.021	2.639SerTyr: 2.639 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	2.829ThrAla: 2.829 ± 0.061	0.531ThrCys: 0.531 ± 0.022	2.423ThrAsp: 2.423 ± 0.043	3.123ThrGlu: 3.123 ± 0.052	2.316ThrPhe: 2.316 ± 0.048	3.776ThrGly: 3.776 ± 0.105	0.728ThrHis: 0.728 ± 0.025	5.138ThrIle: 5.138 ± 0.081	3.91ThrLys: 3.91 ± 0.064	5.015ThrLeu: 5.015 ± 0.07	1.207ThrMet: 1.207 ± 0.031	2.905ThrAsn: 2.905 ± 0.059	1.835ThrPro: 1.835 ± 0.044	1.121ThrGln: 1.121 ± 0.034	1.547ThrArg: 1.547 ± 0.037	3.266ThrSer: 3.266 ± 0.054	2.871ThrThr: 2.871 ± 0.068	3.451ThrVal: 3.451 ± 0.061	0.357ThrTrp: 0.357 ± 0.021	1.888ThrTyr: 1.888 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	4.095ValAla: 4.095 ± 0.074	0.81ValCys: 0.81 ± 0.024	3.837ValAsp: 3.837 ± 0.061	4.573ValGlu: 4.573 ± 0.069	2.797ValPhe: 2.797 ± 0.051	4.369ValGly: 4.369 ± 0.069	0.778ValHis: 0.778 ± 0.025	6.549ValIle: 6.549 ± 0.08	5.549ValLys: 5.549 ± 0.079	5.879ValLeu: 5.879 ± 0.075	1.638ValMet: 1.638 ± 0.034	3.899ValAsn: 3.899 ± 0.061	1.874ValPro: 1.874 ± 0.049	1.318ValGln: 1.318 ± 0.038	1.894ValArg: 1.894 ± 0.043	4.228ValSer: 4.228 ± 0.069	3.434ValThr: 3.434 ± 0.067	4.649ValVal: 4.649 ± 0.074	0.416ValTrp: 0.416 ± 0.019	2.33ValTyr: 2.33 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.381TrpAla: 0.381 ± 0.019	0.092TrpCys: 0.092 ± 0.008	0.427TrpAsp: 0.427 ± 0.022	0.425TrpGlu: 0.425 ± 0.02	0.319TrpPhe: 0.319 ± 0.018	0.516TrpGly: 0.516 ± 0.024	0.112TrpHis: 0.112 ± 0.01	0.693TrpIle: 0.693 ± 0.027	0.499TrpLys: 0.499 ± 0.023	0.563TrpLeu: 0.563 ± 0.024	0.175TrpMet: 0.175 ± 0.014	0.484TrpAsn: 0.484 ± 0.021	0.155TrpPro: 0.155 ± 0.012	0.2TrpGln: 0.2 ± 0.014	0.222TrpArg: 0.222 ± 0.013	0.413TrpSer: 0.413 ± 0.02	0.283TrpThr: 0.283 ± 0.015	0.447TrpVal: 0.447 ± 0.02	0.073TrpTrp: 0.073 ± 0.009	0.273TrpTyr: 0.273 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.856TyrAla: 1.856 ± 0.045	0.554TyrCys: 0.554 ± 0.027	2.267TyrAsp: 2.267 ± 0.055	2.974TyrGlu: 2.974 ± 0.056	1.881TyrPhe: 1.881 ± 0.05	2.593TyrGly: 2.593 ± 0.05	0.501TyrHis: 0.501 ± 0.022	4.038TyrIle: 4.038 ± 0.064	3.995TyrLys: 3.995 ± 0.074	3.789TyrLeu: 3.789 ± 0.061	1.001TyrMet: 1.001 ± 0.029	2.871TyrAsn: 2.871 ± 0.061	1.185TyrPro: 1.185 ± 0.034	0.705TyrGln: 0.705 ± 0.025	1.38TyrArg: 1.38 ± 0.041	2.848TyrSer: 2.848 ± 0.05	2.066TyrThr: 2.066 ± 0.042	2.159TyrVal: 2.159 ± 0.043	0.288TyrTrp: 0.288 ± 0.018	1.858TyrTyr: 1.858 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.01

Statistics based on 3849 proteins (1144006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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