Gossypium mustelinum (Cotton)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91033 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D2ZIG6|A0A5D2ZIG6_GOSMU Uncharacterized protein OS=Gossypium mustelinum OX=34275 GN=E1A91_A05G378100v1 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 5.33NEE4 pKa = 4.14GAKK7 pKa = 9.98QSSKK11 pKa = 10.19MLPFTQLDD19 pKa = 4.6LIDD22 pKa = 4.74SDD24 pKa = 4.58LAVALALQEE33 pKa = 4.04QEE35 pKa = 4.59RR36 pKa = 11.84VFSLLEE42 pKa = 3.81TVEE45 pKa = 3.98RR46 pKa = 11.84DD47 pKa = 3.76GYY49 pKa = 11.56DD50 pKa = 3.22NDD52 pKa = 3.85VDD54 pKa = 4.31DD55 pKa = 6.15DD56 pKa = 4.81SDD58 pKa = 4.31VFHH61 pKa = 7.38DD62 pKa = 3.78QNNNTSYY69 pKa = 11.14EE70 pKa = 4.08YY71 pKa = 10.48IEE73 pKa = 5.53DD74 pKa = 3.94GGNLEE79 pKa = 4.03FLNRR83 pKa = 11.84QDD85 pKa = 4.95SNDD88 pKa = 3.9SEE90 pKa = 5.4DD91 pKa = 5.94DD92 pKa = 3.64YY93 pKa = 12.04DD94 pKa = 6.26NDD96 pKa = 5.86DD97 pKa = 5.79DD98 pKa = 6.36DD99 pKa = 6.87DD100 pKa = 6.06NDD102 pKa = 4.86DD103 pKa = 4.2FEE105 pKa = 7.22HH106 pKa = 7.86DD107 pKa = 3.61IDD109 pKa = 6.17LDD111 pKa = 3.89DD112 pKa = 4.7FSYY115 pKa = 11.59EE116 pKa = 3.79EE117 pKa = 5.0LIALGEE123 pKa = 4.23LIGIEE128 pKa = 3.99RR129 pKa = 11.84RR130 pKa = 11.84GLSEE134 pKa = 4.93NEE136 pKa = 3.67ISSCLVPVKK145 pKa = 10.24FQSIEE150 pKa = 3.94HH151 pKa = 6.15EE152 pKa = 4.21NEE154 pKa = 2.98IDD156 pKa = 3.44RR157 pKa = 11.84CVICQVEE164 pKa = 4.15YY165 pKa = 11.38GEE167 pKa = 4.32NEE169 pKa = 4.11EE170 pKa = 4.88GLVALPNCKK179 pKa = 9.75HH180 pKa = 6.78PYY182 pKa = 10.06HH183 pKa = 7.16LDD185 pKa = 4.27CISKK189 pKa = 8.55WLQMKK194 pKa = 9.25KK195 pKa = 10.16LCPICGTEE203 pKa = 3.77ISSSIHH209 pKa = 4.17QCC211 pKa = 3.23

Molecular weight: 24.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D2VAM8|A0A5D2VAM8_GOSMU Uncharacterized protein OS=Gossypium mustelinum OX=34275 GN=E1A91_D04G061700v1 PE=4 SV=1
MM1 pKa = 7.33VNDD4 pKa = 3.16RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 8.31RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.66RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84KK26 pKa = 8.4RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84IRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84IRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84TEE44 pKa = 3.44KK45 pKa = 10.82

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73065

17968

91033

37160271

29

7144

408.2

45.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.571 ± 0.008

1.915 ± 0.004

5.241 ± 0.006

6.474 ± 0.014

4.26 ± 0.007

6.461 ± 0.008

2.376 ± 0.004

5.347 ± 0.006

6.256 ± 0.009

9.779 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.438 ± 0.003

4.623 ± 0.006

4.931 ± 0.009

3.769 ± 0.006

5.177 ± 0.007

9.086 ± 0.009

4.878 ± 0.005

6.383 ± 0.007

1.265 ± 0.003

2.77 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski