Amino acid dipepetide frequency for Spirosoma endophyticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.448AlaAla: 6.448 ± 0.069	0.679AlaCys: 0.679 ± 0.015	5.026AlaAsp: 5.026 ± 0.048	4.395AlaGlu: 4.395 ± 0.053	3.566AlaPhe: 3.566 ± 0.04	6.202AlaGly: 6.202 ± 0.059	1.405AlaHis: 1.405 ± 0.027	5.138AlaIle: 5.138 ± 0.047	4.058AlaLys: 4.058 ± 0.05	8.013AlaLeu: 8.013 ± 0.062	1.868AlaMet: 1.868 ± 0.032	3.874AlaAsn: 3.874 ± 0.046	2.983AlaPro: 2.983 ± 0.038	3.813AlaGln: 3.813 ± 0.041	3.58AlaArg: 3.58 ± 0.044	5.068AlaSer: 5.068 ± 0.052	5.436AlaThr: 5.436 ± 0.062	5.411AlaVal: 5.411 ± 0.05	0.968AlaTrp: 0.968 ± 0.024	3.068AlaTyr: 3.068 ± 0.031	0.001AlaXaa: 0.001 ± 0.001
Cys	0.486CysAla: 0.486 ± 0.015	0.1CysCys: 0.1 ± 0.007	0.325CysAsp: 0.325 ± 0.012	0.328CysGlu: 0.328 ± 0.014	0.362CysPhe: 0.362 ± 0.012	0.531CysGly: 0.531 ± 0.014	0.209CysHis: 0.209 ± 0.01	0.452CysIle: 0.452 ± 0.014	0.285CysLys: 0.285 ± 0.013	0.862CysLeu: 0.862 ± 0.02	0.147CysMet: 0.147 ± 0.008	0.276CysAsn: 0.276 ± 0.011	0.335CysPro: 0.335 ± 0.011	0.383CysGln: 0.383 ± 0.014	0.37CysArg: 0.37 ± 0.013	0.484CysSer: 0.484 ± 0.016	0.384CysThr: 0.384 ± 0.011	0.454CysVal: 0.454 ± 0.014	0.114CysTrp: 0.114 ± 0.007	0.285CysTyr: 0.285 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	4.278AspAla: 4.278 ± 0.041	0.341AspCys: 0.341 ± 0.011	2.627AspAsp: 2.627 ± 0.041	3.238AspGlu: 3.238 ± 0.041	2.629AspPhe: 2.629 ± 0.038	4.015AspGly: 4.015 ± 0.052	0.977AspHis: 0.977 ± 0.019	3.042AspIle: 3.042 ± 0.038	2.941AspLys: 2.941 ± 0.037	5.17AspLeu: 5.17 ± 0.056	1.098AspMet: 1.098 ± 0.025	2.318AspAsn: 2.318 ± 0.032	2.506AspPro: 2.506 ± 0.031	2.38AspGln: 2.38 ± 0.035	2.835AspArg: 2.835 ± 0.044	2.998AspSer: 2.998 ± 0.042	2.782AspThr: 2.782 ± 0.037	3.814AspVal: 3.814 ± 0.038	0.899AspTrp: 0.899 ± 0.02	2.376AspTyr: 2.376 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	4.324GluAla: 4.324 ± 0.047	0.265GluCys: 0.265 ± 0.011	2.146GluAsp: 2.146 ± 0.032	2.886GluGlu: 2.886 ± 0.04	2.089GluPhe: 2.089 ± 0.029	3.167GluGly: 3.167 ± 0.038	1.001GluHis: 1.001 ± 0.023	3.04GluIle: 3.04 ± 0.039	3.347GluLys: 3.347 ± 0.044	5.427GluLeu: 5.427 ± 0.051	1.218GluMet: 1.218 ± 0.024	2.328GluAsn: 2.328 ± 0.032	1.98GluPro: 1.98 ± 0.035	2.618GluGln: 2.618 ± 0.038	3.11GluArg: 3.11 ± 0.043	2.901GluSer: 2.901 ± 0.035	3.195GluThr: 3.195 ± 0.038	3.396GluVal: 3.396 ± 0.038	0.676GluTrp: 0.676 ± 0.017	1.711GluTyr: 1.711 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.34PheAla: 3.34 ± 0.033	0.426PheCys: 0.426 ± 0.015	2.8PheAsp: 2.8 ± 0.034	2.137PheGlu: 2.137 ± 0.029	2.157PhePhe: 2.157 ± 0.031	3.452PheGly: 3.452 ± 0.039	0.764PheHis: 0.764 ± 0.018	2.625PheIle: 2.625 ± 0.037	2.014PheLys: 2.014 ± 0.03	4.293PheLeu: 4.293 ± 0.048	0.998PheMet: 0.998 ± 0.019	2.113PheAsn: 2.113 ± 0.029	1.848PhePro: 1.848 ± 0.027	1.654PheGln: 1.654 ± 0.028	2.378PheArg: 2.378 ± 0.034	3.363PheSer: 3.363 ± 0.039	3.07PheThr: 3.07 ± 0.039	2.966PheVal: 2.966 ± 0.037	0.656PheTrp: 0.656 ± 0.017	1.755PheTyr: 1.755 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	4.984GlyAla: 4.984 ± 0.051	0.625GlyCys: 0.625 ± 0.02	3.362GlyAsp: 3.362 ± 0.041	3.266GlyGlu: 3.266 ± 0.034	3.455GlyPhe: 3.455 ± 0.035	5.348GlyGly: 5.348 ± 0.065	1.369GlyHis: 1.369 ± 0.029	4.678GlyIle: 4.678 ± 0.045	4.447GlyLys: 4.447 ± 0.044	7.361GlyLeu: 7.361 ± 0.062	1.654GlyMet: 1.654 ± 0.03	3.247GlyAsn: 3.247 ± 0.053	2.212GlyPro: 2.212 ± 0.038	3.362GlyGln: 3.362 ± 0.038	3.509GlyArg: 3.509 ± 0.04	4.616GlySer: 4.616 ± 0.061	4.565GlyThr: 4.565 ± 0.067	4.839GlyVal: 4.839 ± 0.047	1.164GlyTrp: 1.164 ± 0.025	3.067GlyTyr: 3.067 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	1.217HisAla: 1.217 ± 0.028	0.17HisCys: 0.17 ± 0.008	0.889HisAsp: 0.889 ± 0.021	0.985HisGlu: 0.985 ± 0.021	1.023HisPhe: 1.023 ± 0.023	1.14HisGly: 1.14 ± 0.027	0.501HisHis: 0.501 ± 0.014	1.204HisIle: 1.204 ± 0.024	0.842HisLys: 0.842 ± 0.017	1.918HisLeu: 1.918 ± 0.032	0.35HisMet: 0.35 ± 0.012	0.767HisAsn: 0.767 ± 0.016	1.18HisPro: 1.18 ± 0.022	0.974HisGln: 0.974 ± 0.021	1.03HisArg: 1.03 ± 0.022	1.075HisSer: 1.075 ± 0.019	1.095HisThr: 1.095 ± 0.02	1.092HisVal: 1.092 ± 0.02	0.333HisTrp: 0.333 ± 0.012	0.897HisTyr: 0.897 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.043IleAla: 5.043 ± 0.053	0.529IleCys: 0.529 ± 0.015	3.732IleAsp: 3.732 ± 0.037	3.344IleGlu: 3.344 ± 0.043	2.195IlePhe: 2.195 ± 0.036	4.748IleGly: 4.748 ± 0.043	1.165IleHis: 1.165 ± 0.021	3.374IleIle: 3.374 ± 0.05	3.01IleLys: 3.01 ± 0.04	5.189IleLeu: 5.189 ± 0.055	1.024IleMet: 1.024 ± 0.021	3.193IleAsn: 3.193 ± 0.042	2.955IlePro: 2.955 ± 0.032	2.507IleGln: 2.507 ± 0.036	3.526IleArg: 3.526 ± 0.038	4.051IleSer: 4.051 ± 0.04	3.969IleThr: 3.969 ± 0.046	3.843IleVal: 3.843 ± 0.043	0.739IleTrp: 0.739 ± 0.019	2.082IleTyr: 2.082 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	4.757LysAla: 4.757 ± 0.05	0.202LysCys: 0.202 ± 0.009	2.655LysAsp: 2.655 ± 0.035	2.957LysGlu: 2.957 ± 0.045	1.727LysPhe: 1.727 ± 0.027	3.638LysGly: 3.638 ± 0.042	0.935LysHis: 0.935 ± 0.018	2.995LysIle: 2.995 ± 0.038	3.291LysLys: 3.291 ± 0.045	5.112LysLeu: 5.112 ± 0.055	1.322LysMet: 1.322 ± 0.024	2.589LysAsn: 2.589 ± 0.037	2.919LysPro: 2.919 ± 0.035	2.478LysGln: 2.478 ± 0.033	2.758LysArg: 2.758 ± 0.029	3.034LysSer: 3.034 ± 0.042	3.595LysThr: 3.595 ± 0.041	3.282LysVal: 3.282 ± 0.036	0.614LysTrp: 0.614 ± 0.015	1.776LysTyr: 1.776 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	8.697LeuAla: 8.697 ± 0.072	0.763LeuCys: 0.763 ± 0.02	5.005LeuAsp: 5.005 ± 0.048	4.474LeuGlu: 4.474 ± 0.046	4.658LeuPhe: 4.658 ± 0.055	6.416LeuGly: 6.416 ± 0.053	1.843LeuHis: 1.843 ± 0.028	6.289LeuIle: 6.289 ± 0.06	5.404LeuLys: 5.404 ± 0.05	10.974LeuLeu: 10.974 ± 0.098	2.224LeuMet: 2.224 ± 0.033	4.815LeuAsn: 4.815 ± 0.051	5.118LeuPro: 5.118 ± 0.053	3.794LeuGln: 3.794 ± 0.046	5.059LeuArg: 5.059 ± 0.046	7.089LeuSer: 7.089 ± 0.053	7.482LeuThr: 7.482 ± 0.065	6.584LeuVal: 6.584 ± 0.057	1.094LeuTrp: 1.094 ± 0.025	3.41LeuTyr: 3.41 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.093MetAla: 2.093 ± 0.03	0.108MetCys: 0.108 ± 0.007	1.103MetAsp: 1.103 ± 0.021	1.03MetGlu: 1.03 ± 0.021	0.627MetPhe: 0.627 ± 0.016	1.513MetGly: 1.513 ± 0.029	0.451MetHis: 0.451 ± 0.014	1.202MetIle: 1.202 ± 0.025	1.576MetLys: 1.576 ± 0.03	2.11MetLeu: 2.11 ± 0.032	0.521MetMet: 0.521 ± 0.016	1.227MetAsn: 1.227 ± 0.022	1.161MetPro: 1.161 ± 0.024	0.921MetGln: 0.921 ± 0.02	1.104MetArg: 1.104 ± 0.024	1.347MetSer: 1.347 ± 0.025	1.385MetThr: 1.385 ± 0.025	1.295MetVal: 1.295 ± 0.026	0.188MetTrp: 0.188 ± 0.009	0.596MetTyr: 0.596 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.628AsnAla: 3.628 ± 0.047	0.334AsnCys: 0.334 ± 0.018	2.398AsnAsp: 2.398 ± 0.032	2.381AsnGlu: 2.381 ± 0.033	1.996AsnPhe: 1.996 ± 0.031	3.676AsnGly: 3.676 ± 0.045	0.875AsnHis: 0.875 ± 0.022	2.458AsnIle: 2.458 ± 0.037	2.215AsnLys: 2.215 ± 0.033	4.448AsnLeu: 4.448 ± 0.044	0.922AsnMet: 0.922 ± 0.019	2.246AsnAsn: 2.246 ± 0.043	2.848AsnPro: 2.848 ± 0.04	2.467AsnGln: 2.467 ± 0.037	2.702AsnArg: 2.702 ± 0.034	2.76AsnSer: 2.76 ± 0.041	2.901AsnThr: 2.901 ± 0.042	3.292AsnVal: 3.292 ± 0.039	0.716AsnTrp: 0.716 ± 0.016	2.038AsnTyr: 2.038 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	4.169ProAla: 4.169 ± 0.051	0.211ProCys: 0.211 ± 0.01	3.267ProAsp: 3.267 ± 0.045	2.795ProGlu: 2.795 ± 0.034	2.102ProPhe: 2.102 ± 0.029	3.262ProGly: 3.262 ± 0.041	0.795ProHis: 0.795 ± 0.02	2.853ProIle: 2.853 ± 0.036	2.207ProLys: 2.207 ± 0.029	4.082ProLeu: 4.082 ± 0.04	0.972ProMet: 0.972 ± 0.021	2.301ProAsn: 2.301 ± 0.035	1.507ProPro: 1.507 ± 0.033	1.633ProGln: 1.633 ± 0.025	1.604ProArg: 1.604 ± 0.027	2.737ProSer: 2.737 ± 0.039	3.318ProThr: 3.318 ± 0.039	3.661ProVal: 3.661 ± 0.042	0.477ProTrp: 0.477 ± 0.016	1.657ProTyr: 1.657 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.932GlnAla: 3.932 ± 0.042	0.229GlnCys: 0.229 ± 0.009	1.74GlnAsp: 1.74 ± 0.025	2.173GlnGlu: 2.173 ± 0.033	1.978GlnPhe: 1.978 ± 0.031	2.556GlnGly: 2.556 ± 0.034	0.901GlnHis: 0.901 ± 0.021	2.561GlnIle: 2.561 ± 0.03	2.492GlnLys: 2.492 ± 0.035	5.039GlnLeu: 5.039 ± 0.051	0.972GlnMet: 0.972 ± 0.021	1.927GlnAsn: 1.927 ± 0.028	2.428GlnPro: 2.428 ± 0.029	2.648GlnGln: 2.648 ± 0.04	2.449GlnArg: 2.449 ± 0.035	2.685GlnSer: 2.685 ± 0.036	3.099GlnThr: 3.099 ± 0.04	2.866GlnVal: 2.866 ± 0.036	0.565GlnTrp: 0.565 ± 0.014	1.65GlnTyr: 1.65 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	3.52ArgAla: 3.52 ± 0.039	0.308ArgCys: 0.308 ± 0.011	2.459ArgAsp: 2.459 ± 0.037	2.623ArgGlu: 2.623 ± 0.037	2.512ArgPhe: 2.512 ± 0.034	2.715ArgGly: 2.715 ± 0.037	0.981ArgHis: 0.981 ± 0.02	3.505ArgIle: 3.505 ± 0.043	2.761ArgLys: 2.761 ± 0.037	5.525ArgLeu: 5.525 ± 0.049	1.269ArgMet: 1.269 ± 0.021	2.479ArgAsn: 2.479 ± 0.037	2.258ArgPro: 2.258 ± 0.029	2.841ArgGln: 2.841 ± 0.04	2.611ArgArg: 2.611 ± 0.037	3.072ArgSer: 3.072 ± 0.041	3.022ArgThr: 3.022 ± 0.039	3.377ArgVal: 3.377 ± 0.038	0.786ArgTrp: 0.786 ± 0.019	2.253ArgTyr: 2.253 ± 0.031	0.001ArgXaa: 0.001 ± 0.001
Ser	4.993SerAla: 4.993 ± 0.056	0.503SerCys: 0.503 ± 0.015	3.3SerAsp: 3.3 ± 0.04	2.887SerGlu: 2.887 ± 0.038	3.252SerPhe: 3.252 ± 0.038	4.973SerGly: 4.973 ± 0.055	1.113SerHis: 1.113 ± 0.025	3.973SerIle: 3.973 ± 0.042	2.782SerLys: 2.782 ± 0.034	6.994SerLeu: 6.994 ± 0.061	1.283SerMet: 1.283 ± 0.026	2.605SerAsn: 2.605 ± 0.039	2.992SerPro: 2.992 ± 0.039	2.565SerGln: 2.565 ± 0.038	3.049SerArg: 3.049 ± 0.037	4.185SerSer: 4.185 ± 0.051	4.137SerThr: 4.137 ± 0.049	4.623SerVal: 4.623 ± 0.048	0.838SerTrp: 0.838 ± 0.021	2.542SerTyr: 2.542 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	5.333ThrAla: 5.333 ± 0.057	0.38ThrCys: 0.38 ± 0.013	3.919ThrAsp: 3.919 ± 0.04	2.914ThrGlu: 2.914 ± 0.039	2.994ThrPhe: 2.994 ± 0.035	5.285ThrGly: 5.285 ± 0.065	1.17ThrHis: 1.17 ± 0.023	4.159ThrIle: 4.159 ± 0.045	2.965ThrLys: 2.965 ± 0.039	6.711ThrLeu: 6.711 ± 0.06	1.139ThrMet: 1.139 ± 0.022	3.258ThrAsn: 3.258 ± 0.048	3.316ThrPro: 3.316 ± 0.04	2.53ThrGln: 2.53 ± 0.035	2.698ThrArg: 2.698 ± 0.036	4.043ThrSer: 4.043 ± 0.051	4.527ThrThr: 4.527 ± 0.063	4.585ThrVal: 4.585 ± 0.05	0.745ThrTrp: 0.745 ± 0.018	2.793ThrTyr: 2.793 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	5.919ValAla: 5.919 ± 0.055	0.567ValCys: 0.567 ± 0.015	3.744ValAsp: 3.744 ± 0.043	3.418ValGlu: 3.418 ± 0.04	2.923ValPhe: 2.923 ± 0.039	4.85ValGly: 4.85 ± 0.049	1.139ValHis: 1.139 ± 0.024	4.045ValIle: 4.045 ± 0.046	3.44ValLys: 3.44 ± 0.041	6.616ValLeu: 6.616 ± 0.058	1.523ValMet: 1.523 ± 0.028	3.261ValAsn: 3.261 ± 0.04	2.915ValPro: 2.915 ± 0.04	2.577ValGln: 2.577 ± 0.035	3.535ValArg: 3.535 ± 0.041	4.846ValSer: 4.846 ± 0.051	4.345ValThr: 4.345 ± 0.05	5.084ValVal: 5.084 ± 0.052	0.859ValTrp: 0.859 ± 0.019	2.406ValTyr: 2.406 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	0.961TrpAla: 0.961 ± 0.022	0.095TrpCys: 0.095 ± 0.006	0.618TrpAsp: 0.618 ± 0.017	0.636TrpGlu: 0.636 ± 0.017	0.63TrpPhe: 0.63 ± 0.014	0.895TrpGly: 0.895 ± 0.021	0.31TrpHis: 0.31 ± 0.012	0.711TrpIle: 0.711 ± 0.017	0.678TrpLys: 0.678 ± 0.018	1.581TrpLeu: 1.581 ± 0.029	0.352TrpMet: 0.352 ± 0.014	0.627TrpAsn: 0.627 ± 0.018	0.448TrpPro: 0.448 ± 0.014	0.788TrpGln: 0.788 ± 0.02	0.708TrpArg: 0.708 ± 0.017	0.783TrpSer: 0.783 ± 0.018	0.758TrpThr: 0.758 ± 0.019	0.909TrpVal: 0.909 ± 0.023	0.241TrpTrp: 0.241 ± 0.01	0.488TrpTyr: 0.488 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.878TyrAla: 2.878 ± 0.035	0.338TyrCys: 0.338 ± 0.012	2.15TyrAsp: 2.15 ± 0.031	1.956TyrGlu: 1.956 ± 0.025	1.934TyrPhe: 1.934 ± 0.026	2.75TyrGly: 2.75 ± 0.036	0.78TyrHis: 0.78 ± 0.018	1.913TyrIle: 1.913 ± 0.027	1.879TyrLys: 1.879 ± 0.028	3.764TyrLeu: 3.764 ± 0.041	0.701TyrMet: 0.701 ± 0.017	1.85TyrAsn: 1.85 ± 0.033	1.7TyrPro: 1.7 ± 0.025	1.954TyrGln: 1.954 ± 0.03	2.264TyrArg: 2.264 ± 0.033	2.454TyrSer: 2.454 ± 0.033	2.398TyrThr: 2.398 ± 0.037	2.641TyrVal: 2.641 ± 0.034	0.548TyrTrp: 0.548 ± 0.014	1.68TyrTyr: 1.68 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.003

Statistics based on 7570 proteins (2451505 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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