Amino acid dipepetide frequency for Brachybacterium alimentarium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.418AlaAla: 18.418 ± 0.196
0.719AlaCys: 0.719 ± 0.024
8.511AlaAsp: 8.511 ± 0.095
8.28AlaGlu: 8.28 ± 0.1
3.312AlaPhe: 3.312 ± 0.06
11.662AlaGly: 11.662 ± 0.122
2.719AlaHis: 2.719 ± 0.044
5.031AlaIle: 5.031 ± 0.082
2.179AlaLys: 2.179 ± 0.047
14.017AlaLeu: 14.017 ± 0.138
2.837AlaMet: 2.837 ± 0.056
1.757AlaAsn: 1.757 ± 0.039
7.02AlaPro: 7.02 ± 0.103
4.121AlaGln: 4.121 ± 0.059
9.27AlaArg: 9.27 ± 0.105
7.045AlaSer: 7.045 ± 0.081
6.914AlaThr: 6.914 ± 0.084
10.642AlaVal: 10.642 ± 0.096
1.757AlaTrp: 1.757 ± 0.042
2.137AlaTyr: 2.137 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.694CysAla: 0.694 ± 0.025
0.051CysCys: 0.051 ± 0.006
0.339CysAsp: 0.339 ± 0.017
0.307CysGlu: 0.307 ± 0.016
0.165CysPhe: 0.165 ± 0.011
0.674CysGly: 0.674 ± 0.024
0.152CysHis: 0.152 ± 0.012
0.18CysIle: 0.18 ± 0.011
0.05CysLys: 0.05 ± 0.007
0.507CysLeu: 0.507 ± 0.021
0.078CysMet: 0.078 ± 0.007
0.096CysAsn: 0.096 ± 0.01
0.338CysPro: 0.338 ± 0.02
0.131CysGln: 0.131 ± 0.009
0.375CysArg: 0.375 ± 0.019
0.328CysSer: 0.328 ± 0.016
0.363CysThr: 0.363 ± 0.016
0.422CysVal: 0.422 ± 0.021
0.083CysTrp: 0.083 ± 0.008
0.117CysTyr: 0.117 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.57AspAla: 8.57 ± 0.102
0.295AspCys: 0.295 ± 0.015
4.245AspAsp: 4.245 ± 0.071
4.578AspGlu: 4.578 ± 0.06
1.589AspPhe: 1.589 ± 0.041
6.676AspGly: 6.676 ± 0.104
1.564AspHis: 1.564 ± 0.036
2.223AspIle: 2.223 ± 0.045
0.876AspLys: 0.876 ± 0.03
7.287AspLeu: 7.287 ± 0.087
0.966AspMet: 0.966 ± 0.029
0.809AspAsn: 0.809 ± 0.026
4.97AspPro: 4.97 ± 0.071
1.848AspGln: 1.848 ± 0.041
4.443AspArg: 4.443 ± 0.069
3.121AspSer: 3.121 ± 0.054
2.968AspThr: 2.968 ± 0.052
5.285AspVal: 5.285 ± 0.071
0.92AspTrp: 0.92 ± 0.029
1.135AspTyr: 1.135 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
7.306GluAla: 7.306 ± 0.086
0.258GluCys: 0.258 ± 0.014
4.31GluAsp: 4.31 ± 0.069
4.072GluGlu: 4.072 ± 0.063
1.574GluPhe: 1.574 ± 0.041
4.799GluGly: 4.799 ± 0.074
1.934GluHis: 1.934 ± 0.041
2.978GluIle: 2.978 ± 0.05
1.459GluLys: 1.459 ± 0.04
6.666GluLeu: 6.666 ± 0.088
1.113GluMet: 1.113 ± 0.032
1.411GluAsn: 1.411 ± 0.037
3.092GluPro: 3.092 ± 0.051
2.897GluGln: 2.897 ± 0.055
5.118GluArg: 5.118 ± 0.07
3.146GluSer: 3.146 ± 0.053
3.21GluThr: 3.21 ± 0.057
4.999GluVal: 4.999 ± 0.079
0.7GluTrp: 0.7 ± 0.023
1.073GluTyr: 1.073 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.563PheAla: 3.563 ± 0.052
0.214PheCys: 0.214 ± 0.013
2.029PheAsp: 2.029 ± 0.046
1.622PheGlu: 1.622 ± 0.037
0.994PhePhe: 0.994 ± 0.035
2.888PheGly: 2.888 ± 0.054
0.616PheHis: 0.616 ± 0.022
0.937PheIle: 0.937 ± 0.033
0.358PheLys: 0.358 ± 0.017
2.909PheLeu: 2.909 ± 0.055
0.503PheMet: 0.503 ± 0.02
0.514PheAsn: 0.514 ± 0.022
1.357PhePro: 1.357 ± 0.036
0.726PheGln: 0.726 ± 0.026
1.664PheArg: 1.664 ± 0.039
1.828PheSer: 1.828 ± 0.04
1.996PheThr: 1.996 ± 0.044
2.437PheVal: 2.437 ± 0.047
0.448PheTrp: 0.448 ± 0.02
0.61PheTyr: 0.61 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
11.115GlyAla: 11.115 ± 0.116
0.567GlyCys: 0.567 ± 0.022
5.129GlyAsp: 5.129 ± 0.071
5.561GlyGlu: 5.561 ± 0.07
2.811GlyPhe: 2.811 ± 0.051
7.979GlyGly: 7.979 ± 0.114
2.019GlyHis: 2.019 ± 0.041
4.506GlyIle: 4.506 ± 0.069
1.919GlyLys: 1.919 ± 0.042
8.847GlyLeu: 8.847 ± 0.079
2.115GlyMet: 2.115 ± 0.046
1.527GlyAsn: 1.527 ± 0.042
4.513GlyPro: 4.513 ± 0.059
2.584GlyGln: 2.584 ± 0.041
6.582GlyArg: 6.582 ± 0.08
6.027GlySer: 6.027 ± 0.088
6.19GlyThr: 6.19 ± 0.075
7.128GlyVal: 7.128 ± 0.081
1.487GlyTrp: 1.487 ± 0.035
2.032GlyTyr: 2.032 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.488HisAla: 2.488 ± 0.049
0.126HisCys: 0.126 ± 0.011
1.688HisAsp: 1.688 ± 0.043
1.501HisGlu: 1.501 ± 0.033
0.531HisPhe: 0.531 ± 0.02
2.349HisGly: 2.349 ± 0.043
0.699HisHis: 0.699 ± 0.026
0.621HisIle: 0.621 ± 0.022
0.261HisLys: 0.261 ± 0.014
2.639HisLeu: 2.639 ± 0.049
0.294HisMet: 0.294 ± 0.015
0.325HisAsn: 0.325 ± 0.017
1.629HisPro: 1.629 ± 0.035
0.677HisGln: 0.677 ± 0.022
2.17HisArg: 2.17 ± 0.047
1.132HisSer: 1.132 ± 0.034
1.073HisThr: 1.073 ± 0.027
1.789HisVal: 1.789 ± 0.043
0.331HisTrp: 0.331 ± 0.016
0.406HisTyr: 0.406 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
6.355IleAla: 6.355 ± 0.084
0.254IleCys: 0.254 ± 0.015
3.324IleAsp: 3.324 ± 0.064
2.574IleGlu: 2.574 ± 0.052
0.992IlePhe: 0.992 ± 0.033
4.481IleGly: 4.481 ± 0.058
0.799IleHis: 0.799 ± 0.024
1.669IleIle: 1.669 ± 0.042
0.577IleLys: 0.577 ± 0.025
3.576IleLeu: 3.576 ± 0.058
0.674IleMet: 0.674 ± 0.027
0.743IleAsn: 0.743 ± 0.029
2.375IlePro: 2.375 ± 0.045
0.885IleGln: 0.885 ± 0.03
2.383IleArg: 2.383 ± 0.048
2.617IleSer: 2.617 ± 0.047
2.842IleThr: 2.842 ± 0.059
3.818IleVal: 3.818 ± 0.057
0.516IleTrp: 0.516 ± 0.019
0.69IleTyr: 0.69 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
2.0LysAla: 2.0 ± 0.045
0.062LysCys: 0.062 ± 0.007
1.099LysAsp: 1.099 ± 0.03
0.976LysGlu: 0.976 ± 0.033
0.408LysPhe: 0.408 ± 0.019
1.463LysGly: 1.463 ± 0.042
0.435LysHis: 0.435 ± 0.02
0.85LysIle: 0.85 ± 0.028
0.681LysLys: 0.681 ± 0.028
1.525LysLeu: 1.525 ± 0.039
0.334LysMet: 0.334 ± 0.018
0.481LysAsn: 0.481 ± 0.022
0.892LysPro: 0.892 ± 0.036
0.604LysGln: 0.604 ± 0.025
1.198LysArg: 1.198 ± 0.031
0.983LysSer: 0.983 ± 0.034
1.019LysThr: 1.019 ± 0.03
1.416LysVal: 1.416 ± 0.037
0.187LysTrp: 0.187 ± 0.011
0.366LysTyr: 0.366 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
14.274LeuAla: 14.274 ± 0.142
0.584LeuCys: 0.584 ± 0.02
7.024LeuAsp: 7.024 ± 0.078
6.339LeuGlu: 6.339 ± 0.077
2.768LeuPhe: 2.768 ± 0.051
9.457LeuGly: 9.457 ± 0.098
2.325LeuHis: 2.325 ± 0.041
4.23LeuIle: 4.23 ± 0.074
1.38LeuLys: 1.38 ± 0.037
11.449LeuLeu: 11.449 ± 0.129
1.969LeuMet: 1.969 ± 0.039
1.67LeuAsn: 1.67 ± 0.035
6.006LeuPro: 6.006 ± 0.072
2.718LeuGln: 2.718 ± 0.05
7.909LeuArg: 7.909 ± 0.091
6.064LeuSer: 6.064 ± 0.069
6.474LeuThr: 6.474 ± 0.072
9.134LeuVal: 9.134 ± 0.089
1.27LeuTrp: 1.27 ± 0.033
1.608LeuTyr: 1.608 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
2.178MetAla: 2.178 ± 0.041
0.092MetCys: 0.092 ± 0.009
1.039MetAsp: 1.039 ± 0.028
0.802MetGlu: 0.802 ± 0.026
0.603MetPhe: 0.603 ± 0.022
1.462MetGly: 1.462 ± 0.038
0.432MetHis: 0.432 ± 0.019
1.058MetIle: 1.058 ± 0.031
0.371MetLys: 0.371 ± 0.017
2.238MetLeu: 2.238 ± 0.044
0.443MetMet: 0.443 ± 0.019
0.501MetAsn: 0.501 ± 0.022
1.265MetPro: 1.265 ± 0.034
0.509MetGln: 0.509 ± 0.023
1.449MetArg: 1.449 ± 0.034
1.479MetSer: 1.479 ± 0.034
1.807MetThr: 1.807 ± 0.039
1.587MetVal: 1.587 ± 0.036
0.198MetTrp: 0.198 ± 0.013
0.328MetTyr: 0.328 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.04AsnAla: 2.04 ± 0.048
0.092AsnCys: 0.092 ± 0.009
0.944AsnAsp: 0.944 ± 0.033
0.778AsnGlu: 0.778 ± 0.025
0.545AsnPhe: 0.545 ± 0.025
1.569AsnGly: 1.569 ± 0.041
0.366AsnHis: 0.366 ± 0.017
0.776AsnIle: 0.776 ± 0.026
0.284AsnLys: 0.284 ± 0.015
1.709AsnLeu: 1.709 ± 0.041
0.31AsnMet: 0.31 ± 0.016
0.385AsnAsn: 0.385 ± 0.022
1.366AsnPro: 1.366 ± 0.031
0.499AsnGln: 0.499 ± 0.02
1.138AsnArg: 1.138 ± 0.028
0.988AsnSer: 0.988 ± 0.032
1.052AsnThr: 1.052 ± 0.029
1.361AsnVal: 1.361 ± 0.039
0.266AsnTrp: 0.266 ± 0.015
0.439AsnTyr: 0.439 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.421ProAla: 7.421 ± 0.093
0.232ProCys: 0.232 ± 0.016
4.046ProAsp: 4.046 ± 0.058
4.561ProGlu: 4.561 ± 0.066
1.693ProPhe: 1.693 ± 0.037
5.44ProGly: 5.44 ± 0.084
1.327ProHis: 1.327 ± 0.036
1.876ProIle: 1.876 ± 0.042
0.858ProLys: 0.858 ± 0.028
5.271ProLeu: 5.271 ± 0.074
1.043ProMet: 1.043 ± 0.033
0.816ProAsn: 0.816 ± 0.026
2.473ProPro: 2.473 ± 0.055
1.716ProGln: 1.716 ± 0.042
3.739ProArg: 3.739 ± 0.06
3.762ProSer: 3.762 ± 0.057
3.517ProThr: 3.517 ± 0.055
4.734ProVal: 4.734 ± 0.064
0.882ProTrp: 0.882 ± 0.027
1.049ProTyr: 1.049 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.496GlnAla: 3.496 ± 0.063
0.139GlnCys: 0.139 ± 0.013
1.787GlnAsp: 1.787 ± 0.046
1.932GlnGlu: 1.932 ± 0.046
0.846GlnPhe: 0.846 ± 0.025
2.353GlnGly: 2.353 ± 0.052
0.749GlnHis: 0.749 ± 0.025
1.475GlnIle: 1.475 ± 0.034
0.674GlnLys: 0.674 ± 0.025
3.212GlnLeu: 3.212 ± 0.053
0.606GlnMet: 0.606 ± 0.021
0.599GlnAsn: 0.599 ± 0.019
1.393GlnPro: 1.393 ± 0.037
1.4GlnGln: 1.4 ± 0.039
2.499GlnArg: 2.499 ± 0.058
1.509GlnSer: 1.509 ± 0.038
1.389GlnThr: 1.389 ± 0.037
2.647GlnVal: 2.647 ± 0.047
0.493GlnTrp: 0.493 ± 0.02
0.614GlnTyr: 0.614 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
8.869ArgAla: 8.869 ± 0.107
0.383ArgCys: 0.383 ± 0.018
4.284ArgAsp: 4.284 ± 0.059
4.872ArgGlu: 4.872 ± 0.075
2.207ArgPhe: 2.207 ± 0.044
5.581ArgGly: 5.581 ± 0.073
1.759ArgHis: 1.759 ± 0.042
3.562ArgIle: 3.562 ± 0.053
1.223ArgLys: 1.223 ± 0.039
7.252ArgLeu: 7.252 ± 0.095
1.685ArgMet: 1.685 ± 0.037
1.159ArgAsn: 1.159 ± 0.03
4.198ArgPro: 4.198 ± 0.067
2.008ArgGln: 2.008 ± 0.046
7.401ArgArg: 7.401 ± 0.108
4.835ArgSer: 4.835 ± 0.071
5.084ArgThr: 5.084 ± 0.066
5.14ArgVal: 5.14 ± 0.066
1.199ArgTrp: 1.199 ± 0.031
1.428ArgTyr: 1.428 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
7.855SerAla: 7.855 ± 0.093
0.316SerCys: 0.316 ± 0.016
3.375SerAsp: 3.375 ± 0.053
3.054SerGlu: 3.054 ± 0.054
1.878SerPhe: 1.878 ± 0.044
6.066SerGly: 6.066 ± 0.071
1.116SerHis: 1.116 ± 0.029
2.372SerIle: 2.372 ± 0.045
0.966SerLys: 0.966 ± 0.035
5.727SerLeu: 5.727 ± 0.077
1.419SerMet: 1.419 ± 0.036
0.9SerAsn: 0.9 ± 0.027
3.435SerPro: 3.435 ± 0.055
1.605SerGln: 1.605 ± 0.037
4.408SerArg: 4.408 ± 0.064
4.143SerSer: 4.143 ± 0.066
4.22SerThr: 4.22 ± 0.058
4.673SerVal: 4.673 ± 0.054
1.002SerTrp: 1.002 ± 0.028
1.122SerTyr: 1.122 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
8.209ThrAla: 8.209 ± 0.093
0.31ThrCys: 0.31 ± 0.017
3.731ThrAsp: 3.731 ± 0.056
3.441ThrGlu: 3.441 ± 0.052
1.804ThrPhe: 1.804 ± 0.041
6.08ThrGly: 6.08 ± 0.074
1.183ThrHis: 1.183 ± 0.031
2.57ThrIle: 2.57 ± 0.045
0.968ThrLys: 0.968 ± 0.034
6.102ThrLeu: 6.102 ± 0.075
1.158ThrMet: 1.158 ± 0.03
0.986ThrAsn: 0.986 ± 0.031
3.704ThrPro: 3.704 ± 0.058
1.468ThrGln: 1.468 ± 0.035
3.82ThrArg: 3.82 ± 0.055
3.948ThrSer: 3.948 ± 0.051
4.064ThrThr: 4.064 ± 0.064
5.679ThrVal: 5.679 ± 0.069
0.974ThrTrp: 0.974 ± 0.029
1.194ThrTyr: 1.194 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
9.778ValAla: 9.778 ± 0.091
0.525ValCys: 0.525 ± 0.021
5.381ValAsp: 5.381 ± 0.076
4.924ValGlu: 4.924 ± 0.071
2.379ValPhe: 2.379 ± 0.047
6.851ValGly: 6.851 ± 0.087
1.883ValHis: 1.883 ± 0.038
3.932ValIle: 3.932 ± 0.062
1.321ValLys: 1.321 ± 0.035
10.19ValLeu: 10.19 ± 0.104
1.643ValMet: 1.643 ± 0.039
1.46ValAsn: 1.46 ± 0.036
4.795ValPro: 4.795 ± 0.057
2.317ValGln: 2.317 ± 0.045
5.941ValArg: 5.941 ± 0.079
4.675ValSer: 4.675 ± 0.061
5.19ValThr: 5.19 ± 0.062
7.89ValVal: 7.89 ± 0.096
1.006ValTrp: 1.006 ± 0.029
1.287ValTyr: 1.287 ± 0.033
0.001ValXaa: 0.001 ± 0.001
Trp
1.563TrpAla: 1.563 ± 0.037
0.096TrpCys: 0.096 ± 0.008
0.842TrpAsp: 0.842 ± 0.024
0.804TrpGlu: 0.804 ± 0.023
0.525TrpPhe: 0.525 ± 0.021
1.03TrpGly: 1.03 ± 0.028
0.337TrpHis: 0.337 ± 0.016
0.696TrpIle: 0.696 ± 0.026
0.308TrpLys: 0.308 ± 0.017
1.468TrpLeu: 1.468 ± 0.037
0.35TrpMet: 0.35 ± 0.018
0.408TrpAsn: 0.408 ± 0.02
0.684TrpPro: 0.684 ± 0.028
0.55TrpGln: 0.55 ± 0.023
1.128TrpArg: 1.128 ± 0.031
0.901TrpSer: 0.901 ± 0.028
0.968TrpThr: 0.968 ± 0.028
1.091TrpVal: 1.091 ± 0.029
0.387TrpTrp: 0.387 ± 0.022
0.287TrpTyr: 0.287 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.157TyrAla: 2.157 ± 0.048
0.135TyrCys: 0.135 ± 0.01
1.241TyrAsp: 1.241 ± 0.031
1.137TyrGlu: 1.137 ± 0.032
0.591TyrPhe: 0.591 ± 0.024
1.775TyrGly: 1.775 ± 0.039
0.313TyrHis: 0.313 ± 0.015
0.604TyrIle: 0.604 ± 0.02
0.265TyrLys: 0.265 ± 0.015
2.063TyrLeu: 2.063 ± 0.046
0.299TyrMet: 0.299 ± 0.017
0.361TyrAsn: 0.361 ± 0.018
0.949TyrPro: 0.949 ± 0.031
0.548TyrGln: 0.548 ± 0.021
1.495TyrArg: 1.495 ± 0.037
1.121TyrSer: 1.121 ± 0.034
1.053TyrThr: 1.053 ± 0.028
1.497TyrVal: 1.497 ± 0.034
0.316TyrTrp: 0.316 ± 0.016
0.456TyrTyr: 0.456 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.001XaaGln: 0.001 ± 0.001
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.001XaaXaa: 0.001 ± 0.001
Statistics based on 3588 proteins (1233535 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski