Ananas comosus (Pineapple) (Ananas ananas)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Bromeliaceae; Bromelioideae; Ananas

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23408 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A199VZN6|A0A199VZN6_ANACO Metaxin OS=Ananas comosus OX=4615 GN=ACMD2_17726 PE=3 SV=1
MM1 pKa = 7.7ACLDD5 pKa = 3.74CMIVLEE11 pKa = 4.86APQEE15 pKa = 4.27NYY17 pKa = 9.98SVRR20 pKa = 11.84QISAYY25 pKa = 8.37GLRR28 pKa = 11.84WVLSFLLVEE37 pKa = 4.62AMAHH41 pKa = 5.32FFYY44 pKa = 11.23YY45 pKa = 10.4NAFAISGVWWQLSPFEE61 pKa = 4.66IFLIGYY67 pKa = 8.33GLEE70 pKa = 4.26TTTEE74 pKa = 3.96EE75 pKa = 4.32TGTGEE80 pKa = 4.09TTFPEE85 pKa = 4.88DD86 pKa = 3.25GAEE89 pKa = 4.08EE90 pKa = 4.26TGTGSGVTGTGSGDD104 pKa = 3.57DD105 pKa = 3.83GAEE108 pKa = 3.96EE109 pKa = 4.37TGTGSGVTGTGSGDD123 pKa = 3.57DD124 pKa = 3.83GAEE127 pKa = 3.91EE128 pKa = 4.42TGTDD132 pKa = 3.39DD133 pKa = 4.89ADD135 pKa = 4.7AIFADD140 pKa = 4.38VSPLRR145 pKa = 11.84SSTSPGPQEE154 pKa = 3.48IALRR158 pKa = 11.84TIKK161 pKa = 9.96MEE163 pKa = 3.99SSQEE167 pKa = 3.81FLHH170 pKa = 7.06AA171 pKa = 4.91

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A199W8J8|A0A199W8J8_ANACO Putative transcriptional regulator RABBIT EARS (Fragment) OS=Ananas comosus OX=4615 GN=ACMD2_14472 PE=4 SV=1
VV1 pKa = 6.35KK2 pKa = 10.22RR3 pKa = 11.84VRR5 pKa = 11.84SRR7 pKa = 11.84KK8 pKa = 9.4GKK10 pKa = 9.61LRR12 pKa = 11.84SIGPPPKK19 pKa = 9.07KK20 pKa = 8.85TSFSSFVVRR29 pKa = 11.84PPHH32 pKa = 6.0LAIVV36 pKa = 3.86

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23192

216

23408

9336427

8

4290

398.9

44.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.28 ± 0.02

1.817 ± 0.008

5.188 ± 0.011

6.455 ± 0.017

4.0 ± 0.011

6.966 ± 0.014

2.321 ± 0.007

4.897 ± 0.011

5.337 ± 0.016

9.944 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.259 ± 0.006

3.886 ± 0.013

5.384 ± 0.015

3.272 ± 0.012

6.164 ± 0.016

8.785 ± 0.016

4.589 ± 0.01

6.479 ± 0.012

1.285 ± 0.006

2.691 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski