Phlebia centrifuga

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Meruliaceae; Phlebia

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2R6S5A0|A0A2R6S5A0_9APHY Uncharacterized protein OS=Phlebia centrifuga OX=98765 GN=PHLCEN_2v674 PE=4 SV=1
MM1 pKa = 6.85STEE4 pKa = 4.14TTSLFSLARR13 pKa = 11.84NKK15 pKa = 10.16LQSVVGGGVKK25 pKa = 10.27DD26 pKa = 3.34SCSLHH31 pKa = 6.14RR32 pKa = 11.84WVLLKK37 pKa = 10.98NSIIRR42 pKa = 11.84SHH44 pKa = 7.15PPLAAHH50 pKa = 7.34DD51 pKa = 4.12ATDD54 pKa = 3.5EE55 pKa = 4.18ADD57 pKa = 3.35ADD59 pKa = 3.62SVYY62 pKa = 10.63RR63 pKa = 11.84HH64 pKa = 5.92EE65 pKa = 4.62EE66 pKa = 4.09EE67 pKa = 4.24EE68 pKa = 4.2SLEE71 pKa = 4.05EE72 pKa = 4.54DD73 pKa = 3.67EE74 pKa = 5.6QDD76 pKa = 2.92TFMFPDD82 pKa = 3.68PHH84 pKa = 7.52YY85 pKa = 10.13LLPDD89 pKa = 3.48EE90 pKa = 4.98TPAAGIDD97 pKa = 3.36EE98 pKa = 4.41RR99 pKa = 11.84QWLDD103 pKa = 3.16SLLEE107 pKa = 4.12GLEE110 pKa = 4.3DD111 pKa = 6.16DD112 pKa = 5.49EE113 pKa = 7.72DD114 pKa = 5.35DD115 pKa = 6.05DD116 pKa = 4.85YY117 pKa = 12.3SKK119 pKa = 11.74LDD121 pKa = 3.7GQLSALPTLATDD133 pKa = 4.88DD134 pKa = 3.76VAEE137 pKa = 4.61PLSPLYY143 pKa = 11.14SPMSSSDD150 pKa = 3.76DD151 pKa = 3.9LADD154 pKa = 3.35QSNYY158 pKa = 9.4FSYY161 pKa = 10.42PMPYY165 pKa = 9.65PPPHH169 pKa = 7.11PPLIPSWFEE178 pKa = 3.42LQAPSDD184 pKa = 4.03SALTSPVILCDD195 pKa = 3.64NPLPYY200 pKa = 10.49YY201 pKa = 10.78AVDD204 pKa = 4.12DD205 pKa = 5.09LEE207 pKa = 4.96DD208 pKa = 4.56LPVPDD213 pKa = 5.89AIEE216 pKa = 4.42DD217 pKa = 3.94TSDD220 pKa = 4.1DD221 pKa = 3.89EE222 pKa = 5.66SDD224 pKa = 3.84APSTPSATSTSSLAPINSSSISTPPGRR251 pKa = 11.84TRR253 pKa = 11.84FLSDD257 pKa = 3.05PQVYY261 pKa = 10.34VDD263 pKa = 3.9TDD265 pKa = 3.51DD266 pKa = 3.36SHH268 pKa = 7.36FYY270 pKa = 10.54PFEE273 pKa = 5.14LDD275 pKa = 3.53PLPFPGPYY283 pKa = 9.43HH284 pKa = 6.95AGVV287 pKa = 3.25

Molecular weight:
31.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2R6NYK5|A0A2R6NYK5_9APHY Uncharacterized protein OS=Phlebia centrifuga OX=98765 GN=PHLCEN_2v6923 PE=4 SV=1
MM1 pKa = 7.49AVIAKK6 pKa = 9.61IALAATGLARR16 pKa = 11.84SSKK19 pKa = 9.99PGRR22 pKa = 11.84ILNRR26 pKa = 11.84VVNQIARR33 pKa = 11.84MGVLVRR39 pKa = 11.84VLTWPKK45 pKa = 9.43YY46 pKa = 8.21PRR48 pKa = 11.84SGRR51 pKa = 11.84PLSRR55 pKa = 11.84LKK57 pKa = 10.93AA58 pKa = 3.68

Molecular weight:
6.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13628

0

13628

5081813

49

3910

372.9

41.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.38 ± 0.02

1.163 ± 0.008

5.789 ± 0.014

6.184 ± 0.022

3.598 ± 0.014

6.389 ± 0.02

2.532 ± 0.011

4.857 ± 0.015

4.612 ± 0.02

9.113 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.009

3.421 ± 0.012

6.565 ± 0.024

3.788 ± 0.014

6.209 ± 0.018

8.851 ± 0.029

6.044 ± 0.013

6.359 ± 0.018

1.403 ± 0.007

2.6 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski