Equine encephalosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6CFI5|S6CFI5_9REOV VP2 protein OS=Equine encephalosis virus OX=201490 GN=VP2 PE=4 SV=1
MM1 pKa = 7.73DD2 pKa = 6.41AIAARR7 pKa = 11.84ALSVVRR13 pKa = 11.84ACVTAQDD20 pKa = 4.38PRR22 pKa = 11.84ATMAPQVLEE31 pKa = 3.84ILGLGINRR39 pKa = 11.84YY40 pKa = 8.24NAITGSRR47 pKa = 11.84VTMRR51 pKa = 11.84PGTVPEE57 pKa = 4.3RR58 pKa = 11.84NDD60 pKa = 3.29MMFMCIDD67 pKa = 4.0MVCAALNIQLGNVSPDD83 pKa = 3.26YY84 pKa = 9.14TQKK87 pKa = 11.27LEE89 pKa = 4.45TIGVLATSEE98 pKa = 3.76IPYY101 pKa = 9.69TDD103 pKa = 3.43EE104 pKa = 5.07AINAIVRR111 pKa = 11.84VTGEE115 pKa = 4.03TQTWGPVDD123 pKa = 4.11SPMPPYY129 pKa = 10.39LGAVAVEE136 pKa = 4.3APGHH140 pKa = 5.29YY141 pKa = 10.07FMPLGRR147 pKa = 11.84QTHH150 pKa = 5.12SAYY153 pKa = 9.56TDD155 pKa = 3.43SNTIQVSLTPNSAAQVNGAMTPQGVEE181 pKa = 3.81LVQLFFVWRR190 pKa = 11.84PFAMYY195 pKa = 9.57TSQAGAAVAQPPGVTLSLGGVNMPEE220 pKa = 3.64GRR222 pKa = 11.84ILIWDD227 pKa = 4.16GVAPVVVQNPGNAPAMAQIEE247 pKa = 4.73VVRR250 pKa = 11.84FTQLLHH256 pKa = 7.54SYY258 pKa = 7.59EE259 pKa = 4.46TVPEE263 pKa = 4.09TRR265 pKa = 11.84EE266 pKa = 3.76NMARR270 pKa = 11.84CYY272 pKa = 11.01SFLSVTWHH280 pKa = 5.58TLRR283 pKa = 11.84SAILRR288 pKa = 11.84SLGMLPVHH296 pKa = 6.37QSAYY300 pKa = 9.69PPTNRR305 pKa = 11.84YY306 pKa = 8.91EE307 pKa = 4.0YY308 pKa = 10.77LAYY311 pKa = 10.96LLIAALADD319 pKa = 3.88AYY321 pKa = 10.83DD322 pKa = 3.81ALRR325 pKa = 11.84PNFDD329 pKa = 4.04MFNQPNVPLQPTRR342 pKa = 11.84AQIAALYY349 pKa = 9.69RR350 pKa = 3.85

Molecular weight: 38.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6CGJ8|S6CGJ8_9REOV Outer capsid protein VP5 OS=Equine encephalosis virus OX=201490 GN=VP5 PE=3 SV=1
MM1 pKa = 7.13YY2 pKa = 9.04PVLSRR7 pKa = 11.84AVVGNPEE14 pKa = 3.6EE15 pKa = 4.24RR16 pKa = 11.84ALMVYY21 pKa = 10.09PPTAPMPPVTTWDD34 pKa = 3.43NLKK37 pKa = 9.91IDD39 pKa = 4.4SVDD42 pKa = 3.35GMKK45 pKa = 10.74DD46 pKa = 3.01LALNILDD53 pKa = 4.36KK54 pKa = 11.23NITSTTGADD63 pKa = 3.23EE64 pKa = 4.07CDD66 pKa = 3.51KK67 pKa = 11.19RR68 pKa = 11.84EE69 pKa = 3.95KK70 pKa = 11.46AMFASVAEE78 pKa = 4.39SAADD82 pKa = 3.41SPMVRR87 pKa = 11.84TIKK90 pKa = 9.83IQIYY94 pKa = 10.44NRR96 pKa = 11.84VLDD99 pKa = 3.92DD100 pKa = 3.46MEE102 pKa = 4.8RR103 pKa = 11.84EE104 pKa = 3.8KK105 pKa = 10.98RR106 pKa = 11.84KK107 pKa = 9.64CEE109 pKa = 3.58KK110 pKa = 9.93RR111 pKa = 11.84RR112 pKa = 11.84AMLRR116 pKa = 11.84FVSNAFITLMLMSTFLMAMMQTPPITQYY144 pKa = 10.69VEE146 pKa = 4.19KK147 pKa = 10.28ACNGTGGTEE156 pKa = 4.17TNDD159 pKa = 3.3PCGLMRR165 pKa = 11.84WSGAVQFLMLIMSGFLYY182 pKa = 9.47MCKK185 pKa = 9.67RR186 pKa = 11.84WITTLSTNADD196 pKa = 4.29RR197 pKa = 11.84ISKK200 pKa = 10.51NILKK204 pKa = 10.14RR205 pKa = 11.84RR206 pKa = 11.84AYY208 pKa = 9.69IDD210 pKa = 3.39AARR213 pKa = 11.84SNPNATVLTVTGGNTGDD230 pKa = 4.18LPYY233 pKa = 10.86QFGDD237 pKa = 3.51TAHH240 pKa = 6.87

Molecular weight: 26.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6257

240

1307

625.7

70.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.249 ± 0.633

1.406 ± 0.252

5.849 ± 0.21

6.856 ± 0.404

3.964 ± 0.333

6.601 ± 0.666

2.317 ± 0.287

6.505 ± 0.331

5.258 ± 0.604

8.471 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.516 ± 0.209

4.235 ± 0.137

4.475 ± 0.376

3.5 ± 0.284

7.032 ± 0.215

6.585 ± 0.515

5.514 ± 0.317

7.08 ± 0.271

1.167 ± 0.242

3.42 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski