Amino acid dipepetide frequency for Novibacillus thermophilus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.196AlaAla: 7.196 ± 0.11	0.749AlaCys: 0.749 ± 0.034	4.28AlaAsp: 4.28 ± 0.069	5.666AlaGlu: 5.666 ± 0.081	3.553AlaPhe: 3.553 ± 0.061	5.95AlaGly: 5.95 ± 0.1	1.677AlaHis: 1.677 ± 0.04	5.099AlaIle: 5.099 ± 0.075	4.041AlaLys: 4.041 ± 0.061	8.514AlaLeu: 8.514 ± 0.117	2.189AlaMet: 2.189 ± 0.053	2.486AlaAsn: 2.486 ± 0.058	2.421AlaPro: 2.421 ± 0.054	2.634AlaGln: 2.634 ± 0.056	4.053AlaArg: 4.053 ± 0.065	4.216AlaSer: 4.216 ± 0.066	3.701AlaThr: 3.701 ± 0.065	7.325AlaVal: 7.325 ± 0.114	0.885AlaTrp: 0.885 ± 0.032	2.546AlaTyr: 2.546 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.576CysAla: 0.576 ± 0.027	0.1CysCys: 0.1 ± 0.012	0.478CysAsp: 0.478 ± 0.025	0.492CysGlu: 0.492 ± 0.022	0.321CysPhe: 0.321 ± 0.02	0.775CysGly: 0.775 ± 0.028	0.246CysHis: 0.246 ± 0.016	0.459CysIle: 0.459 ± 0.024	0.249CysLys: 0.249 ± 0.016	0.798CysLeu: 0.798 ± 0.031	0.168CysMet: 0.168 ± 0.013	0.214CysAsn: 0.214 ± 0.014	0.404CysPro: 0.404 ± 0.022	0.281CysGln: 0.281 ± 0.022	0.506CysArg: 0.506 ± 0.023	0.487CysSer: 0.487 ± 0.023	0.44CysThr: 0.44 ± 0.02	0.579CysVal: 0.579 ± 0.027	0.101CysTrp: 0.101 ± 0.009	0.26CysTyr: 0.26 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.981AspAla: 3.981 ± 0.065	0.427AspCys: 0.427 ± 0.021	3.056AspAsp: 3.056 ± 0.063	4.393AspGlu: 4.393 ± 0.077	2.094AspPhe: 2.094 ± 0.045	4.265AspGly: 4.265 ± 0.082	1.252AspHis: 1.252 ± 0.035	3.77AspIle: 3.77 ± 0.066	2.378AspLys: 2.378 ± 0.052	4.958AspLeu: 4.958 ± 0.07	1.35AspMet: 1.35 ± 0.034	1.651AspAsn: 1.651 ± 0.044	2.509AspPro: 2.509 ± 0.054	1.722AspGln: 1.722 ± 0.042	3.45AspArg: 3.45 ± 0.061	2.432AspSer: 2.432 ± 0.057	2.638AspThr: 2.638 ± 0.056	5.371AspVal: 5.371 ± 0.085	0.818AspTrp: 0.818 ± 0.032	1.937AspTyr: 1.937 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	6.339GluAla: 6.339 ± 0.102	0.404GluCys: 0.404 ± 0.021	3.791GluAsp: 3.791 ± 0.071	6.265GluGlu: 6.265 ± 0.116	1.92GluPhe: 1.92 ± 0.044	4.745GluGly: 4.745 ± 0.075	1.782GluHis: 1.782 ± 0.04	4.084GluIle: 4.084 ± 0.071	4.963GluLys: 4.963 ± 0.074	6.711GluLeu: 6.711 ± 0.086	2.091GluMet: 2.091 ± 0.042	2.634GluAsn: 2.634 ± 0.058	2.452GluPro: 2.452 ± 0.046	3.632GluGln: 3.632 ± 0.068	5.449GluArg: 5.449 ± 0.086	3.161GluSer: 3.161 ± 0.054	4.095GluThr: 4.095 ± 0.075	4.907GluVal: 4.907 ± 0.076	1.085GluTrp: 1.085 ± 0.038	1.898GluTyr: 1.898 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.078PheAla: 3.078 ± 0.063	0.366PheCys: 0.366 ± 0.018	2.372PheAsp: 2.372 ± 0.056	2.258PheGlu: 2.258 ± 0.046	2.034PhePhe: 2.034 ± 0.054	3.203PheGly: 3.203 ± 0.06	1.024PheHis: 1.024 ± 0.035	2.628PheIle: 2.628 ± 0.062	1.732PheLys: 1.732 ± 0.046	4.132PheLeu: 4.132 ± 0.08	0.969PheMet: 0.969 ± 0.03	1.4PheAsn: 1.4 ± 0.038	1.728PhePro: 1.728 ± 0.047	1.421PheGln: 1.421 ± 0.045	2.224PheArg: 2.224 ± 0.045	2.683PheSer: 2.683 ± 0.056	2.348PheThr: 2.348 ± 0.049	3.571PheVal: 3.571 ± 0.061	0.528PheTrp: 0.528 ± 0.028	1.415PheTyr: 1.415 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	5.437GlyAla: 5.437 ± 0.096	0.711GlyCys: 0.711 ± 0.031	3.839GlyAsp: 3.839 ± 0.074	5.284GlyGlu: 5.284 ± 0.076	3.153GlyPhe: 3.153 ± 0.068	5.332GlyGly: 5.332 ± 0.082	1.698GlyHis: 1.698 ± 0.045	5.405GlyIle: 5.405 ± 0.09	4.367GlyLys: 4.367 ± 0.073	7.014GlyLeu: 7.014 ± 0.101	2.305GlyMet: 2.305 ± 0.053	2.478GlyAsn: 2.478 ± 0.056	2.162GlyPro: 2.162 ± 0.052	2.415GlyGln: 2.415 ± 0.049	3.848GlyArg: 3.848 ± 0.073	3.911GlySer: 3.911 ± 0.064	4.431GlyThr: 4.431 ± 0.069	6.03GlyVal: 6.03 ± 0.088	1.117GlyTrp: 1.117 ± 0.039	2.683GlyTyr: 2.683 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.64HisAla: 1.64 ± 0.044	0.251HisCys: 0.251 ± 0.017	1.236HisAsp: 1.236 ± 0.036	1.357HisGlu: 1.357 ± 0.04	1.167HisPhe: 1.167 ± 0.035	1.568HisGly: 1.568 ± 0.042	0.713HisHis: 0.713 ± 0.033	1.576HisIle: 1.576 ± 0.041	0.93HisLys: 0.93 ± 0.033	2.501HisLeu: 2.501 ± 0.05	0.631HisMet: 0.631 ± 0.026	0.787HisAsn: 0.787 ± 0.03	1.506HisPro: 1.506 ± 0.04	0.762HisGln: 0.762 ± 0.026	1.361HisArg: 1.361 ± 0.037	1.144HisSer: 1.144 ± 0.033	1.18HisThr: 1.18 ± 0.035	2.038HisVal: 2.038 ± 0.051	0.314HisTrp: 0.314 ± 0.019	0.953HisTyr: 0.953 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.506IleAla: 5.506 ± 0.083	0.521IleCys: 0.521 ± 0.028	3.536IleAsp: 3.536 ± 0.066	4.338IleGlu: 4.338 ± 0.076	2.257IlePhe: 2.257 ± 0.059	5.331IleGly: 5.331 ± 0.087	1.492IleHis: 1.492 ± 0.043	3.08IleIle: 3.08 ± 0.069	2.619IleLys: 2.619 ± 0.057	5.445IleLeu: 5.445 ± 0.085	1.326IleMet: 1.326 ± 0.041	1.917IleAsn: 1.917 ± 0.053	3.298IlePro: 3.298 ± 0.067	2.423IleGln: 2.423 ± 0.047	3.691IleArg: 3.691 ± 0.065	3.482IleSer: 3.482 ± 0.074	3.094IleThr: 3.094 ± 0.062	5.717IleVal: 5.717 ± 0.077	0.625IleTrp: 0.625 ± 0.027	1.729IleTyr: 1.729 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.106LysAla: 4.106 ± 0.064	0.28LysCys: 0.28 ± 0.018	2.743LysAsp: 2.743 ± 0.062	4.429LysGlu: 4.429 ± 0.08	1.272LysPhe: 1.272 ± 0.037	3.835LysGly: 3.835 ± 0.067	1.175LysHis: 1.175 ± 0.033	2.888LysIle: 2.888 ± 0.061	3.515LysLys: 3.515 ± 0.065	4.478LysLeu: 4.478 ± 0.066	1.475LysMet: 1.475 ± 0.041	1.794LysAsn: 1.794 ± 0.048	2.138LysPro: 2.138 ± 0.055	2.498LysGln: 2.498 ± 0.06	4.074LysArg: 4.074 ± 0.063	2.568LysSer: 2.568 ± 0.053	2.863LysThr: 2.863 ± 0.053	4.122LysVal: 4.122 ± 0.063	0.815LysTrp: 0.815 ± 0.032	1.459LysTyr: 1.459 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	8.283LeuAla: 8.283 ± 0.11	0.8LeuCys: 0.8 ± 0.032	5.24LeuAsp: 5.24 ± 0.072	6.451LeuGlu: 6.451 ± 0.092	4.508LeuPhe: 4.508 ± 0.093	6.704LeuGly: 6.704 ± 0.104	2.113LeuHis: 2.113 ± 0.046	6.064LeuIle: 6.064 ± 0.098	5.65LeuLys: 5.65 ± 0.087	10.037LeuLeu: 10.037 ± 0.133	2.481LeuMet: 2.481 ± 0.052	3.723LeuAsn: 3.723 ± 0.066	4.396LeuPro: 4.396 ± 0.07	3.52LeuGln: 3.52 ± 0.069	4.877LeuArg: 4.877 ± 0.078	6.647LeuSer: 6.647 ± 0.091	5.838LeuThr: 5.838 ± 0.071	6.971LeuVal: 6.971 ± 0.086	0.98LeuTrp: 0.98 ± 0.04	3.066LeuTyr: 3.066 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.416MetAla: 2.416 ± 0.058	0.194MetCys: 0.194 ± 0.014	1.411MetAsp: 1.411 ± 0.038	1.815MetGlu: 1.815 ± 0.047	0.958MetPhe: 0.958 ± 0.033	1.906MetGly: 1.906 ± 0.045	0.497MetHis: 0.497 ± 0.022	1.584MetIle: 1.584 ± 0.046	1.653MetLys: 1.653 ± 0.043	2.393MetLeu: 2.393 ± 0.054	0.818MetMet: 0.818 ± 0.033	1.118MetAsn: 1.118 ± 0.031	0.999MetPro: 0.999 ± 0.032	0.888MetGln: 0.888 ± 0.032	1.584MetArg: 1.584 ± 0.039	1.633MetSer: 1.633 ± 0.037	1.704MetThr: 1.704 ± 0.04	1.874MetVal: 1.874 ± 0.045	0.291MetTrp: 0.291 ± 0.018	0.692MetTyr: 0.692 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.544AsnAla: 2.544 ± 0.054	0.239AsnCys: 0.239 ± 0.016	1.636AsnAsp: 1.636 ± 0.039	2.324AsnGlu: 2.324 ± 0.055	1.156AsnPhe: 1.156 ± 0.037	2.878AsnGly: 2.878 ± 0.062	0.679AsnHis: 0.679 ± 0.026	2.325AsnIle: 2.325 ± 0.057	1.52AsnLys: 1.52 ± 0.047	2.967AsnLeu: 2.967 ± 0.058	0.945AsnMet: 0.945 ± 0.031	1.096AsnAsn: 1.096 ± 0.042	1.88AsnPro: 1.88 ± 0.047	1.199AsnGln: 1.199 ± 0.037	2.489AsnArg: 2.489 ± 0.052	1.545AsnSer: 1.545 ± 0.04	1.645AsnThr: 1.645 ± 0.041	3.243AsnVal: 3.243 ± 0.059	0.556AsnTrp: 0.556 ± 0.023	0.995AsnTyr: 0.995 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	2.723ProAla: 2.723 ± 0.055	0.272ProCys: 0.272 ± 0.018	2.832ProAsp: 2.832 ± 0.056	3.566ProGlu: 3.566 ± 0.065	2.093ProPhe: 2.093 ± 0.053	3.057ProGly: 3.057 ± 0.059	1.135ProHis: 1.135 ± 0.038	2.265ProIle: 2.265 ± 0.056	1.969ProLys: 1.969 ± 0.047	4.217ProLeu: 4.217 ± 0.064	0.907ProMet: 0.907 ± 0.032	1.451ProAsn: 1.451 ± 0.039	1.549ProPro: 1.549 ± 0.04	1.328ProGln: 1.328 ± 0.038	1.829ProArg: 1.829 ± 0.043	2.493ProSer: 2.493 ± 0.054	2.024ProThr: 2.024 ± 0.043	3.696ProVal: 3.696 ± 0.067	0.55ProTrp: 0.55 ± 0.023	1.568ProTyr: 1.568 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	3.223GlnAla: 3.223 ± 0.063	0.222GlnCys: 0.222 ± 0.015	1.647GlnAsp: 1.647 ± 0.043	2.532GlnGlu: 2.532 ± 0.063	1.537GlnPhe: 1.537 ± 0.044	2.28GlnGly: 2.28 ± 0.048	0.967GlnHis: 0.967 ± 0.036	1.995GlnIle: 1.995 ± 0.043	2.33GlnLys: 2.33 ± 0.058	4.245GlnLeu: 4.245 ± 0.077	1.11GlnMet: 1.11 ± 0.037	1.299GlnAsn: 1.299 ± 0.042	1.61GlnPro: 1.61 ± 0.042	1.884GlnGln: 1.884 ± 0.071	2.2GlnArg: 2.2 ± 0.059	2.109GlnSer: 2.109 ± 0.051	2.046GlnThr: 2.046 ± 0.049	2.447GlnVal: 2.447 ± 0.053	0.493GlnTrp: 0.493 ± 0.023	1.225GlnTyr: 1.225 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	3.88ArgAla: 3.88 ± 0.068	0.423ArgCys: 0.423 ± 0.02	3.293ArgAsp: 3.293 ± 0.062	5.001ArgGlu: 5.001 ± 0.078	2.378ArgPhe: 2.378 ± 0.049	3.529ArgGly: 3.529 ± 0.064	1.638ArgHis: 1.638 ± 0.046	3.606ArgIle: 3.606 ± 0.061	3.328ArgLys: 3.328 ± 0.061	6.042ArgLeu: 6.042 ± 0.086	1.664ArgMet: 1.664 ± 0.043	1.908ArgAsn: 1.908 ± 0.039	2.104ArgPro: 2.104 ± 0.046	2.739ArgGln: 2.739 ± 0.059	3.77ArgArg: 3.77 ± 0.076	2.984ArgSer: 2.984 ± 0.061	2.845ArgThr: 2.845 ± 0.054	4.459ArgVal: 4.459 ± 0.062	0.788ArgTrp: 0.788 ± 0.03	2.2ArgTyr: 2.2 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	3.998SerAla: 3.998 ± 0.066	0.413SerCys: 0.413 ± 0.019	2.977SerAsp: 2.977 ± 0.058	3.78SerGlu: 3.78 ± 0.066	2.611SerPhe: 2.611 ± 0.058	4.672SerGly: 4.672 ± 0.078	1.322SerHis: 1.322 ± 0.033	3.247SerIle: 3.247 ± 0.062	2.456SerLys: 2.456 ± 0.061	5.722SerLeu: 5.722 ± 0.078	1.406SerMet: 1.406 ± 0.039	1.603SerAsn: 1.603 ± 0.04	2.399SerPro: 2.399 ± 0.056	1.887SerGln: 1.887 ± 0.049	3.228SerArg: 3.228 ± 0.063	3.223SerSer: 3.223 ± 0.062	2.783SerThr: 2.783 ± 0.053	4.734SerVal: 4.734 ± 0.073	0.725SerTrp: 0.725 ± 0.027	1.739SerTyr: 1.739 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	4.531ThrAla: 4.531 ± 0.077	0.419ThrCys: 0.419 ± 0.022	2.855ThrAsp: 2.855 ± 0.061	3.498ThrGlu: 3.498 ± 0.067	2.647ThrPhe: 2.647 ± 0.055	4.625ThrGly: 4.625 ± 0.066	1.21ThrHis: 1.21 ± 0.034	3.372ThrIle: 3.372 ± 0.068	2.242ThrLys: 2.242 ± 0.051	5.686ThrLeu: 5.686 ± 0.065	1.302ThrMet: 1.302 ± 0.035	1.618ThrAsn: 1.618 ± 0.044	2.573ThrPro: 2.573 ± 0.046	1.421ThrGln: 1.421 ± 0.038	2.382ThrArg: 2.382 ± 0.048	2.944ThrSer: 2.944 ± 0.057	2.799ThrThr: 2.799 ± 0.072	5.571ThrVal: 5.571 ± 0.083	0.654ThrTrp: 0.654 ± 0.031	1.836ThrTyr: 1.836 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	6.39ValAla: 6.39 ± 0.089	0.752ValCys: 0.752 ± 0.029	4.568ValAsp: 4.568 ± 0.073	5.849ValGlu: 5.849 ± 0.078	3.419ValPhe: 3.419 ± 0.061	5.546ValGly: 5.546 ± 0.091	1.937ValHis: 1.937 ± 0.043	5.332ValIle: 5.332 ± 0.098	4.513ValLys: 4.513 ± 0.075	8.081ValLeu: 8.081 ± 0.114	2.147ValMet: 2.147 ± 0.051	3.032ValAsn: 3.032 ± 0.059	3.512ValPro: 3.512 ± 0.071	3.029ValGln: 3.029 ± 0.063	4.492ValArg: 4.492 ± 0.07	4.895ValSer: 4.895 ± 0.08	5.19ValThr: 5.19 ± 0.073	6.779ValVal: 6.779 ± 0.098	0.971ValTrp: 0.971 ± 0.034	2.638ValTyr: 2.638 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.838TrpAla: 0.838 ± 0.03	0.104TrpCys: 0.104 ± 0.011	0.699TrpAsp: 0.699 ± 0.027	0.885TrpGlu: 0.885 ± 0.032	0.589TrpPhe: 0.589 ± 0.025	0.824TrpGly: 0.824 ± 0.034	0.328TrpHis: 0.328 ± 0.02	0.827TrpIle: 0.827 ± 0.03	0.764TrpLys: 0.764 ± 0.028	1.432TrpLeu: 1.432 ± 0.045	0.432TrpMet: 0.432 ± 0.021	0.546TrpAsn: 0.546 ± 0.026	0.399TrpPro: 0.399 ± 0.02	0.596TrpGln: 0.596 ± 0.024	0.734TrpArg: 0.734 ± 0.029	0.714TrpSer: 0.714 ± 0.027	0.715TrpThr: 0.715 ± 0.029	0.922TrpVal: 0.922 ± 0.031	0.219TrpTrp: 0.219 ± 0.017	0.369TrpTyr: 0.369 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.498TyrAla: 2.498 ± 0.056	0.287TyrCys: 0.287 ± 0.017	1.962TyrAsp: 1.962 ± 0.05	2.223TyrGlu: 2.223 ± 0.056	1.448TyrPhe: 1.448 ± 0.038	2.599TyrGly: 2.599 ± 0.057	0.733TyrHis: 0.733 ± 0.024	1.823TyrIle: 1.823 ± 0.04	1.307TyrLys: 1.307 ± 0.042	2.974TyrLeu: 2.974 ± 0.065	0.712TyrMet: 0.712 ± 0.031	1.105TyrAsn: 1.105 ± 0.032	1.484TyrPro: 1.484 ± 0.039	1.122TyrGln: 1.122 ± 0.039	2.337TyrArg: 2.337 ± 0.051	1.654TyrSer: 1.654 ± 0.045	1.803TyrThr: 1.803 ± 0.046	2.719TyrVal: 2.719 ± 0.05	0.419TyrTrp: 0.419 ± 0.021	1.259TyrTyr: 1.259 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3169 proteins (951385 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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