Thioclava atlantica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thioclava

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3767 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085TTQ9|A0A085TTQ9_9RHOB Integrase family protein OS=Thioclava atlantica OX=1317124 GN=DW2_13935 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.41VLFATTALVLSAGVAAAEE21 pKa = 4.25VSVGGDD27 pKa = 2.67GRR29 pKa = 11.84MGVMYY34 pKa = 10.63NGSDD38 pKa = 2.94WNFNSRR44 pKa = 11.84IRR46 pKa = 11.84AKK48 pKa = 9.97FTMSGQTDD56 pKa = 3.39GGLEE60 pKa = 4.0FGGSVRR66 pKa = 11.84ADD68 pKa = 3.11QATNATSGTSASVYY82 pKa = 10.11ISGAFGKK89 pKa = 10.65VSMGDD94 pKa = 3.23VVGASEE100 pKa = 5.28ALFGDD105 pKa = 4.43LAEE108 pKa = 4.58VGYY111 pKa = 9.56TDD113 pKa = 5.8LDD115 pKa = 3.95GAGLGNISGLSVGASRR131 pKa = 11.84NNMGANEE138 pKa = 4.0IPYY141 pKa = 9.04LTGDD145 pKa = 3.12NGGSVVGSTANTGPNLLYY163 pKa = 10.3TGTFGAFSVAASASDD178 pKa = 3.36GKK180 pKa = 10.9AVSTSDD186 pKa = 3.29NQEE189 pKa = 3.72YY190 pKa = 10.9ALAAAYY196 pKa = 9.42TMGDD200 pKa = 3.51YY201 pKa = 10.73TIGAGYY207 pKa = 10.04EE208 pKa = 4.11IVDD211 pKa = 3.72VTTGQNWKK219 pKa = 9.78QGEE222 pKa = 4.39IVGTATFGNTAVKK235 pKa = 10.07AYY237 pKa = 10.07YY238 pKa = 10.73ADD240 pKa = 3.66GSGGNNLDD248 pKa = 3.14KK249 pKa = 10.88AYY251 pKa = 10.2GIGVSSVFGATTVNGYY267 pKa = 9.99VNYY270 pKa = 11.03AEE272 pKa = 4.78ATDD275 pKa = 4.09GTNGTAYY282 pKa = 10.44GIGAAYY288 pKa = 10.27DD289 pKa = 3.73LGGGASVVGGVADD302 pKa = 3.87SDD304 pKa = 3.68IAGTDD309 pKa = 3.35TQADD313 pKa = 3.89LGIKK317 pKa = 10.64FSFF320 pKa = 3.88

Molecular weight: 31.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085U0Z7|A0A085U0Z7_9RHOB Tryptophan synthase alpha chain OS=Thioclava atlantica OX=1317124 GN=trpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.5GGRR28 pKa = 11.84KK29 pKa = 8.99VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3767

0

3767

1170515

19

5975

310.7

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.706 ± 0.059

0.809 ± 0.012

5.672 ± 0.037

6.323 ± 0.045

3.645 ± 0.028

8.807 ± 0.046

2.046 ± 0.019

5.182 ± 0.031

3.267 ± 0.035

10.251 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.715 ± 0.02

2.377 ± 0.024

5.264 ± 0.034

3.062 ± 0.023

7.097 ± 0.053

5.043 ± 0.034

5.134 ± 0.037

7.076 ± 0.034

1.406 ± 0.017

2.118 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski