Amino acid dipepetide frequency for Gordonia phage GRU3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.065AlaAla: 23.065 ± 3.004
0.744AlaCys: 0.744 ± 0.356
8.557AlaAsp: 8.557 ± 1.171
5.766AlaGlu: 5.766 ± 0.788
4.092AlaPhe: 4.092 ± 0.922
8.929AlaGly: 8.929 ± 1.452
2.79AlaHis: 2.79 ± 0.654
8.929AlaIle: 8.929 ± 2.377
4.464AlaLys: 4.464 ± 0.93
10.975AlaLeu: 10.975 ± 1.375
4.278AlaMet: 4.278 ± 0.925
4.278AlaAsn: 4.278 ± 0.666
6.324AlaPro: 6.324 ± 1.836
4.65AlaGln: 4.65 ± 0.936
9.115AlaArg: 9.115 ± 1.403
5.952AlaSer: 5.952 ± 0.657
7.999AlaThr: 7.999 ± 0.789
13.579AlaVal: 13.579 ± 2.929
2.976AlaTrp: 2.976 ± 0.461
3.534AlaTyr: 3.534 ± 0.568
0.0AlaXaa: 0.0 ± 0.0
Cys
0.93CysAla: 0.93 ± 0.34
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.558CysGlu: 0.558 ± 0.354
0.186CysPhe: 0.186 ± 0.184
0.93CysGly: 0.93 ± 0.465
0.186CysHis: 0.186 ± 0.198
0.0CysIle: 0.0 ± 0.0
0.558CysLys: 0.558 ± 0.234
0.744CysLeu: 0.744 ± 0.44
0.0CysMet: 0.0 ± 0.0
0.186CysAsn: 0.186 ± 0.198
0.372CysPro: 0.372 ± 0.278
0.744CysGln: 0.744 ± 0.338
1.302CysArg: 1.302 ± 0.587
0.372CysSer: 0.372 ± 0.266
0.372CysThr: 0.372 ± 0.278
0.186CysVal: 0.186 ± 0.174
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.371AspAla: 8.371 ± 1.717
0.744AspCys: 0.744 ± 0.318
5.952AspAsp: 5.952 ± 1.359
2.976AspGlu: 2.976 ± 0.791
1.86AspPhe: 1.86 ± 0.531
5.58AspGly: 5.58 ± 0.755
1.116AspHis: 1.116 ± 0.449
2.046AspIle: 2.046 ± 0.681
1.488AspLys: 1.488 ± 0.485
5.208AspLeu: 5.208 ± 0.773
2.046AspMet: 2.046 ± 0.693
1.488AspAsn: 1.488 ± 0.477
4.092AspPro: 4.092 ± 0.813
1.86AspGln: 1.86 ± 0.808
5.952AspArg: 5.952 ± 0.938
2.604AspSer: 2.604 ± 0.74
3.162AspThr: 3.162 ± 0.995
4.092AspVal: 4.092 ± 1.32
1.488AspTrp: 1.488 ± 0.62
1.674AspTyr: 1.674 ± 0.589
0.0AspXaa: 0.0 ± 0.0
Glu
5.022GluAla: 5.022 ± 1.315
0.372GluCys: 0.372 ± 0.233
1.302GluAsp: 1.302 ± 0.53
0.186GluGlu: 0.186 ± 0.187
2.046GluPhe: 2.046 ± 0.692
3.162GluGly: 3.162 ± 1.105
1.674GluHis: 1.674 ± 0.45
2.418GluIle: 2.418 ± 0.605
0.558GluLys: 0.558 ± 0.463
4.65GluLeu: 4.65 ± 1.182
0.93GluMet: 0.93 ± 0.426
1.488GluAsn: 1.488 ± 0.416
2.604GluPro: 2.604 ± 0.746
1.674GluGln: 1.674 ± 0.613
2.79GluArg: 2.79 ± 0.879
2.232GluSer: 2.232 ± 0.618
3.162GluThr: 3.162 ± 1.137
2.976GluVal: 2.976 ± 0.611
0.558GluTrp: 0.558 ± 0.336
0.558GluTyr: 0.558 ± 0.296
0.0GluXaa: 0.0 ± 0.0
Phe
2.79PheAla: 2.79 ± 0.748
0.186PheCys: 0.186 ± 0.198
3.162PheAsp: 3.162 ± 0.617
0.93PheGlu: 0.93 ± 0.404
0.372PhePhe: 0.372 ± 0.227
4.092PheGly: 4.092 ± 0.907
0.372PheHis: 0.372 ± 0.249
1.116PheIle: 1.116 ± 0.411
0.744PheLys: 0.744 ± 0.336
1.488PheLeu: 1.488 ± 0.519
0.372PheMet: 0.372 ± 0.277
1.302PheAsn: 1.302 ± 0.533
0.744PhePro: 0.744 ± 0.372
1.116PheGln: 1.116 ± 0.397
1.674PheArg: 1.674 ± 0.497
1.674PheSer: 1.674 ± 0.577
1.86PheThr: 1.86 ± 0.441
2.604PheVal: 2.604 ± 0.89
0.93PheTrp: 0.93 ± 0.429
0.558PheTyr: 0.558 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
8.371GlyAla: 8.371 ± 1.491
0.372GlyCys: 0.372 ± 0.227
3.162GlyAsp: 3.162 ± 1.0
3.534GlyGlu: 3.534 ± 0.999
3.162GlyPhe: 3.162 ± 0.653
7.44GlyGly: 7.44 ± 1.156
1.674GlyHis: 1.674 ± 0.575
4.092GlyIle: 4.092 ± 1.184
3.906GlyLys: 3.906 ± 0.746
7.254GlyLeu: 7.254 ± 1.014
1.86GlyMet: 1.86 ± 0.431
2.976GlyAsn: 2.976 ± 0.613
4.65GlyPro: 4.65 ± 0.628
2.79GlyGln: 2.79 ± 0.66
6.138GlyArg: 6.138 ± 0.818
3.906GlySer: 3.906 ± 0.939
7.254GlyThr: 7.254 ± 1.46
6.882GlyVal: 6.882 ± 1.383
1.86GlyTrp: 1.86 ± 0.554
2.418GlyTyr: 2.418 ± 0.508
0.0GlyXaa: 0.0 ± 0.0
His
3.162HisAla: 3.162 ± 0.815
0.0HisCys: 0.0 ± 0.0
0.93HisAsp: 0.93 ± 0.348
0.0HisGlu: 0.0 ± 0.0
0.558HisPhe: 0.558 ± 0.302
1.488HisGly: 1.488 ± 0.784
0.0HisHis: 0.0 ± 0.0
0.558HisIle: 0.558 ± 0.46
0.372HisLys: 0.372 ± 0.289
2.79HisLeu: 2.79 ± 0.586
0.372HisMet: 0.372 ± 0.247
0.372HisAsn: 0.372 ± 0.267
1.116HisPro: 1.116 ± 0.366
0.186HisGln: 0.186 ± 0.183
1.674HisArg: 1.674 ± 0.442
0.744HisSer: 0.744 ± 0.476
2.046HisThr: 2.046 ± 0.618
1.116HisVal: 1.116 ± 0.441
0.186HisTrp: 0.186 ± 0.203
0.372HisTyr: 0.372 ± 0.273
0.0HisXaa: 0.0 ± 0.0
Ile
7.626IleAla: 7.626 ± 1.58
0.186IleCys: 0.186 ± 0.183
4.278IleAsp: 4.278 ± 0.704
3.348IleGlu: 3.348 ± 0.606
0.93IlePhe: 0.93 ± 0.424
4.836IleGly: 4.836 ± 1.258
0.744IleHis: 0.744 ± 0.444
3.162IleIle: 3.162 ± 0.779
1.86IleLys: 1.86 ± 0.741
3.162IleLeu: 3.162 ± 0.611
0.372IleMet: 0.372 ± 0.212
1.302IleAsn: 1.302 ± 0.467
4.092IlePro: 4.092 ± 0.807
1.302IleGln: 1.302 ± 0.603
2.79IleArg: 2.79 ± 0.634
2.604IleSer: 2.604 ± 0.681
3.906IleThr: 3.906 ± 1.013
2.79IleVal: 2.79 ± 0.781
1.116IleTrp: 1.116 ± 0.498
0.93IleTyr: 0.93 ± 0.405
0.0IleXaa: 0.0 ± 0.0
Lys
3.72LysAla: 3.72 ± 1.118
0.744LysCys: 0.744 ± 0.467
0.93LysAsp: 0.93 ± 0.37
1.488LysGlu: 1.488 ± 0.582
0.744LysPhe: 0.744 ± 0.349
2.604LysGly: 2.604 ± 0.435
0.744LysHis: 0.744 ± 0.298
0.558LysIle: 0.558 ± 0.385
0.93LysLys: 0.93 ± 0.412
2.604LysLeu: 2.604 ± 0.416
0.744LysMet: 0.744 ± 0.389
1.488LysAsn: 1.488 ± 0.67
1.302LysPro: 1.302 ± 0.51
0.558LysGln: 0.558 ± 0.269
1.86LysArg: 1.86 ± 0.613
1.116LysSer: 1.116 ± 0.554
2.79LysThr: 2.79 ± 0.836
2.418LysVal: 2.418 ± 0.451
0.372LysTrp: 0.372 ± 0.229
0.558LysTyr: 0.558 ± 0.335
0.0LysXaa: 0.0 ± 0.0
Leu
10.603LeuAla: 10.603 ± 1.995
0.744LeuCys: 0.744 ± 0.398
5.766LeuAsp: 5.766 ± 1.6
1.86LeuGlu: 1.86 ± 0.629
1.674LeuPhe: 1.674 ± 0.655
9.301LeuGly: 9.301 ± 1.577
1.116LeuHis: 1.116 ± 0.451
3.162LeuIle: 3.162 ± 1.026
1.302LeuLys: 1.302 ± 0.404
4.65LeuLeu: 4.65 ± 0.89
2.418LeuMet: 2.418 ± 0.479
2.976LeuAsn: 2.976 ± 0.821
4.65LeuPro: 4.65 ± 0.835
0.558LeuGln: 0.558 ± 0.267
3.534LeuArg: 3.534 ± 1.186
5.766LeuSer: 5.766 ± 0.953
5.766LeuThr: 5.766 ± 1.197
6.51LeuVal: 6.51 ± 0.984
1.674LeuTrp: 1.674 ± 0.523
1.302LeuTyr: 1.302 ± 0.559
0.0LeuXaa: 0.0 ± 0.0
Met
3.534MetAla: 3.534 ± 0.737
0.372MetCys: 0.372 ± 0.296
0.744MetAsp: 0.744 ± 0.348
0.186MetGlu: 0.186 ± 0.203
0.93MetPhe: 0.93 ± 0.45
0.93MetGly: 0.93 ± 0.384
0.372MetHis: 0.372 ± 0.248
1.116MetIle: 1.116 ± 0.543
0.744MetLys: 0.744 ± 0.337
1.674MetLeu: 1.674 ± 0.655
0.186MetMet: 0.186 ± 0.208
0.93MetAsn: 0.93 ± 0.397
1.674MetPro: 1.674 ± 0.617
0.186MetGln: 0.186 ± 0.152
1.116MetArg: 1.116 ± 0.537
3.534MetSer: 3.534 ± 0.663
2.046MetThr: 2.046 ± 0.667
0.93MetVal: 0.93 ± 0.433
0.372MetTrp: 0.372 ± 0.283
0.186MetTyr: 0.186 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
3.906AsnAla: 3.906 ± 0.799
0.186AsnCys: 0.186 ± 0.198
2.604AsnAsp: 2.604 ± 0.938
1.488AsnGlu: 1.488 ± 0.354
0.186AsnPhe: 0.186 ± 0.155
5.022AsnGly: 5.022 ± 1.365
0.558AsnHis: 0.558 ± 0.295
1.488AsnIle: 1.488 ± 0.586
1.302AsnLys: 1.302 ± 0.464
1.116AsnLeu: 1.116 ± 0.405
0.558AsnMet: 0.558 ± 0.309
1.302AsnAsn: 1.302 ± 0.576
2.418AsnPro: 2.418 ± 0.653
0.186AsnGln: 0.186 ± 0.152
2.604AsnArg: 2.604 ± 0.78
2.046AsnSer: 2.046 ± 0.548
1.86AsnThr: 1.86 ± 0.576
1.674AsnVal: 1.674 ± 0.566
0.558AsnTrp: 0.558 ± 0.353
0.93AsnTyr: 0.93 ± 0.445
0.0AsnXaa: 0.0 ± 0.0
Pro
14.137ProAla: 14.137 ± 2.696
0.186ProCys: 0.186 ± 0.184
5.022ProAsp: 5.022 ± 0.864
1.488ProGlu: 1.488 ± 0.572
1.302ProPhe: 1.302 ± 0.426
5.766ProGly: 5.766 ± 0.916
0.93ProHis: 0.93 ± 0.472
2.79ProIle: 2.79 ± 0.618
0.93ProLys: 0.93 ± 0.448
4.65ProLeu: 4.65 ± 1.172
1.116ProMet: 1.116 ± 0.453
1.302ProAsn: 1.302 ± 0.68
4.278ProPro: 4.278 ± 0.891
1.488ProGln: 1.488 ± 0.541
2.604ProArg: 2.604 ± 0.972
2.232ProSer: 2.232 ± 0.532
2.976ProThr: 2.976 ± 0.655
5.208ProVal: 5.208 ± 1.297
1.488ProTrp: 1.488 ± 0.534
1.674ProTyr: 1.674 ± 0.487
0.0ProXaa: 0.0 ± 0.0
Gln
2.976GlnAla: 2.976 ± 0.696
0.186GlnCys: 0.186 ± 0.174
0.558GlnAsp: 0.558 ± 0.346
0.93GlnGlu: 0.93 ± 0.334
0.93GlnPhe: 0.93 ± 0.447
1.302GlnGly: 1.302 ± 0.58
0.744GlnHis: 0.744 ± 0.387
2.046GlnIle: 2.046 ± 0.5
0.372GlnLys: 0.372 ± 0.305
3.534GlnLeu: 3.534 ± 0.833
0.93GlnMet: 0.93 ± 0.466
0.186GlnAsn: 0.186 ± 0.174
1.302GlnPro: 1.302 ± 0.358
0.558GlnGln: 0.558 ± 0.33
1.86GlnArg: 1.86 ± 0.433
1.86GlnSer: 1.86 ± 0.512
2.232GlnThr: 2.232 ± 0.635
2.232GlnVal: 2.232 ± 0.534
0.186GlnTrp: 0.186 ± 0.187
0.558GlnTyr: 0.558 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
8.185ArgAla: 8.185 ± 1.543
0.558ArgCys: 0.558 ± 0.453
3.906ArgAsp: 3.906 ± 0.794
3.534ArgGlu: 3.534 ± 1.118
1.488ArgPhe: 1.488 ± 0.448
5.022ArgGly: 5.022 ± 0.994
1.302ArgHis: 1.302 ± 0.602
3.162ArgIle: 3.162 ± 0.67
1.86ArgLys: 1.86 ± 0.663
6.138ArgLeu: 6.138 ± 1.024
1.488ArgMet: 1.488 ± 0.666
1.302ArgAsn: 1.302 ± 0.51
6.138ArgPro: 6.138 ± 1.764
1.116ArgGln: 1.116 ± 0.453
7.254ArgArg: 7.254 ± 1.661
4.092ArgSer: 4.092 ± 0.965
4.278ArgThr: 4.278 ± 0.934
5.952ArgVal: 5.952 ± 1.176
0.93ArgTrp: 0.93 ± 0.393
2.046ArgTyr: 2.046 ± 0.637
0.0ArgXaa: 0.0 ± 0.0
Ser
7.44SerAla: 7.44 ± 1.115
0.186SerCys: 0.186 ± 0.203
3.162SerAsp: 3.162 ± 0.859
2.418SerGlu: 2.418 ± 0.595
1.302SerPhe: 1.302 ± 0.361
3.534SerGly: 3.534 ± 0.845
1.488SerHis: 1.488 ± 0.461
3.72SerIle: 3.72 ± 0.716
1.674SerLys: 1.674 ± 0.536
3.162SerLeu: 3.162 ± 0.982
1.488SerMet: 1.488 ± 0.436
1.302SerAsn: 1.302 ± 0.455
3.534SerPro: 3.534 ± 0.704
1.302SerGln: 1.302 ± 0.516
3.906SerArg: 3.906 ± 0.855
4.65SerSer: 4.65 ± 1.221
4.092SerThr: 4.092 ± 0.968
4.65SerVal: 4.65 ± 0.713
0.93SerTrp: 0.93 ± 0.496
1.116SerTyr: 1.116 ± 0.479
0.0SerXaa: 0.0 ± 0.0
Thr
10.975ThrAla: 10.975 ± 0.915
0.0ThrCys: 0.0 ± 0.0
4.65ThrAsp: 4.65 ± 1.04
2.79ThrGlu: 2.79 ± 0.887
2.604ThrPhe: 2.604 ± 0.818
5.58ThrGly: 5.58 ± 0.882
0.372ThrHis: 0.372 ± 0.236
3.72ThrIle: 3.72 ± 1.166
1.674ThrLys: 1.674 ± 0.584
3.534ThrLeu: 3.534 ± 0.984
0.744ThrMet: 0.744 ± 0.251
2.418ThrAsn: 2.418 ± 0.648
5.022ThrPro: 5.022 ± 0.906
1.674ThrGln: 1.674 ± 0.436
5.208ThrArg: 5.208 ± 1.159
3.534ThrSer: 3.534 ± 1.048
4.836ThrThr: 4.836 ± 1.018
7.999ThrVal: 7.999 ± 1.296
2.79ThrTrp: 2.79 ± 0.872
0.93ThrTyr: 0.93 ± 0.463
0.0ThrXaa: 0.0 ± 0.0
Val
11.161ValAla: 11.161 ± 1.44
1.302ValCys: 1.302 ± 0.483
6.324ValAsp: 6.324 ± 0.982
4.65ValGlu: 4.65 ± 1.12
2.418ValPhe: 2.418 ± 0.645
5.208ValGly: 5.208 ± 1.281
0.744ValHis: 0.744 ± 0.433
5.58ValIle: 5.58 ± 1.358
2.604ValLys: 2.604 ± 0.498
5.208ValLeu: 5.208 ± 0.893
0.744ValMet: 0.744 ± 0.364
3.162ValAsn: 3.162 ± 0.978
4.65ValPro: 4.65 ± 0.759
2.232ValGln: 2.232 ± 0.849
4.836ValArg: 4.836 ± 1.084
3.72ValSer: 3.72 ± 0.744
6.696ValThr: 6.696 ± 1.168
7.626ValVal: 7.626 ± 1.41
2.046ValTrp: 2.046 ± 0.724
1.302ValTyr: 1.302 ± 0.474
0.0ValXaa: 0.0 ± 0.0
Trp
2.79TrpAla: 2.79 ± 0.693
0.0TrpCys: 0.0 ± 0.0
1.116TrpAsp: 1.116 ± 0.466
1.116TrpGlu: 1.116 ± 0.41
0.558TrpPhe: 0.558 ± 0.274
1.116TrpGly: 1.116 ± 0.659
0.372TrpHis: 0.372 ± 0.425
1.116TrpIle: 1.116 ± 0.394
0.744TrpLys: 0.744 ± 0.317
1.86TrpLeu: 1.86 ± 0.584
0.186TrpMet: 0.186 ± 0.174
1.674TrpAsn: 1.674 ± 0.913
1.302TrpPro: 1.302 ± 0.624
0.744TrpGln: 0.744 ± 0.324
1.488TrpArg: 1.488 ± 0.514
1.488TrpSer: 1.488 ± 0.448
1.86TrpThr: 1.86 ± 0.458
0.558TrpVal: 0.558 ± 0.339
0.0TrpTrp: 0.0 ± 0.0
1.116TrpTyr: 1.116 ± 0.545
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.418TyrAla: 2.418 ± 0.767
0.558TyrCys: 0.558 ± 0.275
1.674TyrAsp: 1.674 ± 0.579
1.116TyrGlu: 1.116 ± 0.433
0.744TyrPhe: 0.744 ± 0.265
1.86TyrGly: 1.86 ± 0.584
0.744TyrHis: 0.744 ± 0.32
0.558TyrIle: 0.558 ± 0.288
0.186TyrLys: 0.186 ± 0.198
0.93TyrLeu: 0.93 ± 0.364
0.744TyrMet: 0.744 ± 0.357
0.558TyrAsn: 0.558 ± 0.325
1.302TyrPro: 1.302 ± 0.544
0.558TyrGln: 0.558 ± 0.26
2.046TyrArg: 2.046 ± 0.678
0.93TyrSer: 0.93 ± 0.431
1.86TyrThr: 1.86 ± 0.65
2.232TyrVal: 2.232 ± 0.699
0.744TyrTrp: 0.744 ± 0.307
0.558TyrTyr: 0.558 ± 0.275
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 26 proteins (5377 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski