Amino acid dipepetide frequency for bacterium HR16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.925AlaAla: 7.925 ± 0.092	1.048AlaCys: 1.048 ± 0.036	4.537AlaAsp: 4.537 ± 0.067	6.523AlaGlu: 6.523 ± 0.101	3.494AlaPhe: 3.494 ± 0.066	7.525AlaGly: 7.525 ± 0.095	2.218AlaHis: 2.218 ± 0.05	5.262AlaIle: 5.262 ± 0.073	3.075AlaLys: 3.075 ± 0.057	10.391AlaLeu: 10.391 ± 0.143	2.48AlaMet: 2.48 ± 0.053	2.453AlaAsn: 2.453 ± 0.058	4.406AlaPro: 4.406 ± 0.061	4.977AlaGln: 4.977 ± 0.086	7.054AlaArg: 7.054 ± 0.096	4.748AlaSer: 4.748 ± 0.075	4.648AlaThr: 4.648 ± 0.076	8.216AlaVal: 8.216 ± 0.099	1.569AlaTrp: 1.569 ± 0.043	2.744AlaTyr: 2.744 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	1.028CysAla: 1.028 ± 0.041	0.153CysCys: 0.153 ± 0.012	0.583CysAsp: 0.583 ± 0.025	0.674CysGlu: 0.674 ± 0.026	0.397CysPhe: 0.397 ± 0.014	0.948CysGly: 0.948 ± 0.032	0.373CysHis: 0.373 ± 0.027	0.485CysIle: 0.485 ± 0.023	0.269CysLys: 0.269 ± 0.018	0.99CysLeu: 0.99 ± 0.032	0.209CysMet: 0.209 ± 0.014	0.279CysAsn: 0.279 ± 0.018	0.673CysPro: 0.673 ± 0.029	0.311CysGln: 0.311 ± 0.02	0.765CysArg: 0.765 ± 0.028	0.518CysSer: 0.518 ± 0.023	0.602CysThr: 0.602 ± 0.03	0.805CysVal: 0.805 ± 0.025	0.191CysTrp: 0.191 ± 0.014	0.336CysTyr: 0.336 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.854AspAla: 4.854 ± 0.077	0.441AspCys: 0.441 ± 0.024	2.131AspAsp: 2.131 ± 0.047	3.496AspGlu: 3.496 ± 0.059	1.911AspPhe: 1.911 ± 0.039	4.654AspGly: 4.654 ± 0.083	0.786AspHis: 0.786 ± 0.031	2.816AspIle: 2.816 ± 0.056	1.421AspLys: 1.421 ± 0.045	4.59AspLeu: 4.59 ± 0.066	1.085AspMet: 1.085 ± 0.034	1.368AspAsn: 1.368 ± 0.039	2.871AspPro: 2.871 ± 0.057	0.912AspGln: 0.912 ± 0.028	2.924AspArg: 2.924 ± 0.046	2.146AspSer: 2.146 ± 0.043	2.716AspThr: 2.716 ± 0.048	3.958AspVal: 3.958 ± 0.054	1.003AspTrp: 1.003 ± 0.036	1.769AspTyr: 1.769 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	6.535GluAla: 6.535 ± 0.111	0.601GluCys: 0.601 ± 0.025	2.686GluAsp: 2.686 ± 0.053	5.404GluGlu: 5.404 ± 0.095	1.928GluPhe: 1.928 ± 0.043	4.976GluGly: 4.976 ± 0.08	1.693GluHis: 1.693 ± 0.04	3.361GluIle: 3.361 ± 0.068	2.519GluLys: 2.519 ± 0.052	5.67GluLeu: 5.67 ± 0.087	1.906GluMet: 1.906 ± 0.047	1.714GluAsn: 1.714 ± 0.043	3.291GluPro: 3.291 ± 0.057	3.647GluGln: 3.647 ± 0.066	5.838GluArg: 5.838 ± 0.069	2.656GluSer: 2.656 ± 0.05	2.926GluThr: 2.926 ± 0.056	5.614GluVal: 5.614 ± 0.085	1.272GluTrp: 1.272 ± 0.038	1.937GluTyr: 1.937 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.773PheAla: 3.773 ± 0.064	0.465PheCys: 0.465 ± 0.02	1.936PheAsp: 1.936 ± 0.048	1.987PheGlu: 1.987 ± 0.043	1.392PhePhe: 1.392 ± 0.044	3.25PheGly: 3.25 ± 0.062	0.844PheHis: 0.844 ± 0.025	1.601PheIle: 1.601 ± 0.038	0.872PheLys: 0.872 ± 0.032	3.28PheLeu: 3.28 ± 0.061	0.636PheMet: 0.636 ± 0.021	0.979PheAsn: 0.979 ± 0.03	1.845PhePro: 1.845 ± 0.042	1.033PheGln: 1.033 ± 0.034	2.595PheArg: 2.595 ± 0.048	1.936PheSer: 1.936 ± 0.047	2.296PheThr: 2.296 ± 0.048	2.956PheVal: 2.956 ± 0.057	0.746PheTrp: 0.746 ± 0.036	1.171PheTyr: 1.171 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	6.876GlyAla: 6.876 ± 0.087	0.961GlyCys: 0.961 ± 0.031	3.998GlyAsp: 3.998 ± 0.069	5.412GlyGlu: 5.412 ± 0.072	2.957GlyPhe: 2.957 ± 0.062	6.407GlyGly: 6.407 ± 0.103	1.453GlyHis: 1.453 ± 0.041	4.469GlyIle: 4.469 ± 0.073	3.903GlyLys: 3.903 ± 0.076	6.361GlyLeu: 6.361 ± 0.091	2.215GlyMet: 2.215 ± 0.051	2.634GlyAsn: 2.634 ± 0.069	1.753GlyPro: 1.753 ± 0.043	2.623GlyGln: 2.623 ± 0.047	4.835GlyArg: 4.835 ± 0.075	4.133GlySer: 4.133 ± 0.069	4.696GlyThr: 4.696 ± 0.087	7.068GlyVal: 7.068 ± 0.09	1.819GlyTrp: 1.819 ± 0.048	2.933GlyTyr: 2.933 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.129HisAla: 2.129 ± 0.045	0.29HisCys: 0.29 ± 0.017	1.037HisAsp: 1.037 ± 0.032	1.359HisGlu: 1.359 ± 0.034	0.855HisPhe: 0.855 ± 0.029	1.602HisGly: 1.602 ± 0.041	0.75HisHis: 0.75 ± 0.031	1.309HisIle: 1.309 ± 0.033	0.531HisLys: 0.531 ± 0.023	2.519HisLeu: 2.519 ± 0.053	0.273HisMet: 0.273 ± 0.016	0.66HisAsn: 0.66 ± 0.026	1.846HisPro: 1.846 ± 0.042	0.762HisGln: 0.762 ± 0.027	1.906HisArg: 1.906 ± 0.044	1.025HisSer: 1.025 ± 0.034	1.313HisThr: 1.313 ± 0.031	1.635HisVal: 1.635 ± 0.044	0.498HisTrp: 0.498 ± 0.022	0.767HisTyr: 0.767 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.07IleAla: 6.07 ± 0.087	0.483IleCys: 0.483 ± 0.023	2.892IleAsp: 2.892 ± 0.055	3.608IleGlu: 3.608 ± 0.066	1.476IlePhe: 1.476 ± 0.032	4.169IleGly: 4.169 ± 0.063	1.056IleHis: 1.056 ± 0.03	2.305IleIle: 2.305 ± 0.045	1.176IleLys: 1.176 ± 0.036	4.791IleLeu: 4.791 ± 0.065	0.77IleMet: 0.77 ± 0.029	1.269IleAsn: 1.269 ± 0.035	3.042IlePro: 3.042 ± 0.05	1.652IleGln: 1.652 ± 0.043	3.718IleArg: 3.718 ± 0.054	2.446IleSer: 2.446 ± 0.05	2.883IleThr: 2.883 ± 0.059	4.059IleVal: 4.059 ± 0.068	0.785IleTrp: 0.785 ± 0.029	1.385IleTyr: 1.385 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	3.101LysAla: 3.101 ± 0.054	0.255LysCys: 0.255 ± 0.018	1.242LysAsp: 1.242 ± 0.036	2.26LysGlu: 2.26 ± 0.056	0.696LysPhe: 0.696 ± 0.026	2.578LysGly: 2.578 ± 0.049	0.798LysHis: 0.798 ± 0.027	1.408LysIle: 1.408 ± 0.04	1.0LysLys: 1.0 ± 0.036	2.814LysLeu: 2.814 ± 0.048	0.863LysMet: 0.863 ± 0.029	0.802LysAsn: 0.802 ± 0.026	2.323LysPro: 2.323 ± 0.056	1.697LysGln: 1.697 ± 0.045	2.842LysArg: 2.842 ± 0.051	1.333LysSer: 1.333 ± 0.032	1.546LysThr: 1.546 ± 0.039	2.821LysVal: 2.821 ± 0.057	0.511LysTrp: 0.511 ± 0.02	0.766LysTyr: 0.766 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	9.76LeuAla: 9.76 ± 0.134	1.207LeuCys: 1.207 ± 0.041	4.255LeuAsp: 4.255 ± 0.06	5.541LeuGlu: 5.541 ± 0.085	3.839LeuPhe: 3.839 ± 0.072	6.699LeuGly: 6.699 ± 0.092	2.509LeuHis: 2.509 ± 0.054	4.496LeuIle: 4.496 ± 0.064	3.268LeuLys: 3.268 ± 0.058	11.169LeuLeu: 11.169 ± 0.153	2.242LeuMet: 2.242 ± 0.047	2.793LeuAsn: 2.793 ± 0.052	6.316LeuPro: 6.316 ± 0.079	5.088LeuGln: 5.088 ± 0.071	8.76LeuArg: 8.76 ± 0.095	6.023LeuSer: 6.023 ± 0.083	5.211LeuThr: 5.211 ± 0.069	7.57LeuVal: 7.57 ± 0.085	1.967LeuTrp: 1.967 ± 0.062	2.827LeuTyr: 2.827 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.207MetAla: 2.207 ± 0.047	0.182MetCys: 0.182 ± 0.013	1.021MetAsp: 1.021 ± 0.032	1.573MetGlu: 1.573 ± 0.04	0.649MetPhe: 0.649 ± 0.027	1.777MetGly: 1.777 ± 0.043	0.521MetHis: 0.521 ± 0.025	0.895MetIle: 0.895 ± 0.026	0.936MetLys: 0.936 ± 0.032	2.472MetLeu: 2.472 ± 0.059	0.651MetMet: 0.651 ± 0.022	0.798MetAsn: 0.798 ± 0.028	1.434MetPro: 1.434 ± 0.036	1.067MetGln: 1.067 ± 0.035	1.973MetArg: 1.973 ± 0.04	1.271MetSer: 1.271 ± 0.034	1.166MetThr: 1.166 ± 0.035	1.837MetVal: 1.837 ± 0.042	0.339MetTrp: 0.339 ± 0.019	0.537MetTyr: 0.537 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.793AsnAla: 2.793 ± 0.053	0.285AsnCys: 0.285 ± 0.021	1.229AsnAsp: 1.229 ± 0.036	1.512AsnGlu: 1.512 ± 0.037	0.908AsnPhe: 0.908 ± 0.033	2.518AsnGly: 2.518 ± 0.065	0.532AsnHis: 0.532 ± 0.025	1.467AsnIle: 1.467 ± 0.039	0.632AsnLys: 0.632 ± 0.026	3.308AsnLeu: 3.308 ± 0.054	0.603AsnMet: 0.603 ± 0.023	0.861AsnAsn: 0.861 ± 0.04	2.288AsnPro: 2.288 ± 0.048	0.764AsnGln: 0.764 ± 0.028	2.139AsnArg: 2.139 ± 0.044	1.172AsnSer: 1.172 ± 0.038	1.619AsnThr: 1.619 ± 0.047	2.169AsnVal: 2.169 ± 0.051	0.531AsnTrp: 0.531 ± 0.026	0.87AsnTyr: 0.87 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	5.232ProAla: 5.232 ± 0.076	0.495ProCys: 0.495 ± 0.024	3.675ProAsp: 3.675 ± 0.068	4.37ProGlu: 4.37 ± 0.069	2.02ProPhe: 2.02 ± 0.044	4.06ProGly: 4.06 ± 0.076	1.238ProHis: 1.238 ± 0.034	2.297ProIle: 2.297 ± 0.048	1.453ProLys: 1.453 ± 0.043	5.152ProLeu: 5.152 ± 0.072	1.166ProMet: 1.166 ± 0.032	1.75ProAsn: 1.75 ± 0.042	3.563ProPro: 3.563 ± 0.067	2.255ProGln: 2.255 ± 0.049	3.292ProArg: 3.292 ± 0.05	2.954ProSer: 2.954 ± 0.046	2.599ProThr: 2.599 ± 0.051	4.829ProVal: 4.829 ± 0.072	0.721ProTrp: 0.721 ± 0.025	1.753ProTyr: 1.753 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.243GlnAla: 4.243 ± 0.074	0.387GlnCys: 0.387 ± 0.019	1.435GlnAsp: 1.435 ± 0.033	2.637GlnGlu: 2.637 ± 0.052	1.394GlnPhe: 1.394 ± 0.035	2.79GlnGly: 2.79 ± 0.048	1.056GlnHis: 1.056 ± 0.029	2.372GlnIle: 2.372 ± 0.047	1.64GlnLys: 1.64 ± 0.042	3.53GlnLeu: 3.53 ± 0.067	1.365GlnMet: 1.365 ± 0.036	1.146GlnAsn: 1.146 ± 0.033	2.633GlnPro: 2.633 ± 0.053	2.563GlnGln: 2.563 ± 0.062	3.532GlnArg: 3.532 ± 0.068	1.947GlnSer: 1.947 ± 0.044	2.177GlnThr: 2.177 ± 0.048	3.589GlnVal: 3.589 ± 0.062	0.825GlnTrp: 0.825 ± 0.03	1.155GlnTyr: 1.155 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	6.767ArgAla: 6.767 ± 0.111	0.812ArgCys: 0.812 ± 0.035	3.316ArgAsp: 3.316 ± 0.055	5.779ArgGlu: 5.779 ± 0.088	3.342ArgPhe: 3.342 ± 0.06	4.701ArgGly: 4.701 ± 0.068	1.644ArgHis: 1.644 ± 0.038	4.152ArgIle: 4.152 ± 0.066	2.587ArgLys: 2.587 ± 0.047	8.305ArgLeu: 8.305 ± 0.099	1.97ArgMet: 1.97 ± 0.04	2.071ArgAsn: 2.071 ± 0.044	2.985ArgPro: 2.985 ± 0.057	3.482ArgGln: 3.482 ± 0.063	6.152ArgArg: 6.152 ± 0.089	3.292ArgSer: 3.292 ± 0.051	3.742ArgThr: 3.742 ± 0.064	6.255ArgVal: 6.255 ± 0.079	1.83ArgTrp: 1.83 ± 0.044	2.963ArgTyr: 2.963 ± 0.058	0.0ArgXaa: 0.0 ± 0.0
Ser	5.087SerAla: 5.087 ± 0.071	0.481SerCys: 0.481 ± 0.024	2.435SerAsp: 2.435 ± 0.056	2.824SerGlu: 2.824 ± 0.057	1.654SerPhe: 1.654 ± 0.039	4.523SerGly: 4.523 ± 0.08	1.111SerHis: 1.111 ± 0.032	2.308SerIle: 2.308 ± 0.044	1.269SerLys: 1.269 ± 0.038	5.443SerLeu: 5.443 ± 0.078	0.992SerMet: 0.992 ± 0.031	1.308SerAsn: 1.308 ± 0.039	3.328SerPro: 3.328 ± 0.065	1.763SerGln: 1.763 ± 0.04	3.409SerArg: 3.409 ± 0.059	2.645SerSer: 2.645 ± 0.053	2.792SerThr: 2.792 ± 0.056	4.094SerVal: 4.094 ± 0.069	0.812SerTrp: 0.812 ± 0.025	1.398SerTyr: 1.398 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	5.067ThrAla: 5.067 ± 0.073	0.533ThrCys: 0.533 ± 0.025	2.712ThrAsp: 2.712 ± 0.051	2.872ThrGlu: 2.872 ± 0.052	1.966ThrPhe: 1.966 ± 0.05	4.832ThrGly: 4.832 ± 0.077	1.206ThrHis: 1.206 ± 0.036	2.708ThrIle: 2.708 ± 0.061	1.107ThrLys: 1.107 ± 0.039	6.196ThrLeu: 6.196 ± 0.087	1.046ThrMet: 1.046 ± 0.033	1.341ThrAsn: 1.341 ± 0.047	3.608ThrPro: 3.608 ± 0.058	1.828ThrGln: 1.828 ± 0.044	3.447ThrArg: 3.447 ± 0.052	2.561ThrSer: 2.561 ± 0.062	2.74ThrThr: 2.74 ± 0.073	4.841ThrVal: 4.841 ± 0.081	0.828ThrTrp: 0.828 ± 0.03	1.549ThrTyr: 1.549 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	7.653ValAla: 7.653 ± 0.096	0.919ValCys: 0.919 ± 0.032	4.05ValAsp: 4.05 ± 0.068	5.371ValGlu: 5.371 ± 0.08	2.845ValPhe: 2.845 ± 0.053	5.6ValGly: 5.6 ± 0.089	1.913ValHis: 1.913 ± 0.044	4.112ValIle: 4.112 ± 0.074	2.638ValLys: 2.638 ± 0.057	8.928ValLeu: 8.928 ± 0.101	1.786ValMet: 1.786 ± 0.041	2.375ValAsn: 2.375 ± 0.054	4.526ValPro: 4.526 ± 0.068	3.655ValGln: 3.655 ± 0.06	6.406ValArg: 6.406 ± 0.084	4.371ValSer: 4.371 ± 0.061	4.574ValThr: 4.574 ± 0.087	7.505ValVal: 7.505 ± 0.11	1.461ValTrp: 1.461 ± 0.041	2.553ValTyr: 2.553 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	1.395TrpAla: 1.395 ± 0.041	0.256TrpCys: 0.256 ± 0.017	0.898TrpAsp: 0.898 ± 0.03	1.064TrpGlu: 1.064 ± 0.032	0.718TrpPhe: 0.718 ± 0.028	1.354TrpGly: 1.354 ± 0.035	0.525TrpHis: 0.525 ± 0.024	0.825TrpIle: 0.825 ± 0.026	0.689TrpLys: 0.689 ± 0.025	2.333TrpLeu: 2.333 ± 0.059	0.522TrpMet: 0.522 ± 0.023	0.717TrpAsn: 0.717 ± 0.026	0.306TrpPro: 0.306 ± 0.018	1.231TrpGln: 1.231 ± 0.035	1.688TrpArg: 1.688 ± 0.048	1.024TrpSer: 1.024 ± 0.038	0.957TrpThr: 0.957 ± 0.033	1.317TrpVal: 1.317 ± 0.04	0.506TrpTrp: 0.506 ± 0.022	0.54TrpTyr: 0.54 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.795TyrAla: 2.795 ± 0.061	0.336TyrCys: 0.336 ± 0.018	1.782TyrAsp: 1.782 ± 0.045	1.904TyrGlu: 1.904 ± 0.044	1.152TyrPhe: 1.152 ± 0.036	2.544TyrGly: 2.544 ± 0.067	0.843TyrHis: 0.843 ± 0.027	1.388TyrIle: 1.388 ± 0.041	0.673TyrLys: 0.673 ± 0.026	3.298TyrLeu: 3.298 ± 0.056	0.525TyrMet: 0.525 ± 0.025	0.883TyrAsn: 0.883 ± 0.032	1.836TyrPro: 1.836 ± 0.04	1.107TyrGln: 1.107 ± 0.034	2.872TyrArg: 2.872 ± 0.055	1.474TyrSer: 1.474 ± 0.035	1.784TyrThr: 1.784 ± 0.048	2.107TyrVal: 2.107 ± 0.045	0.65TyrTrp: 0.65 ± 0.023	1.044TyrTyr: 1.044 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3294 proteins (1088270 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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