Pseudanabaena phage PA-SR01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3I067|A0A6M3I067_9CAUD Uncharacterized protein OS=Pseudanabaena phage PA-SR01 OX=2723892 PE=4 SV=1
MM1 pKa = 7.34SSSNRR6 pKa = 11.84FDD8 pKa = 3.73NLIGKK13 pKa = 7.36TILRR17 pKa = 11.84VEE19 pKa = 4.19EE20 pKa = 4.09QQDD23 pKa = 3.33KK24 pKa = 11.2CSTEE28 pKa = 4.46DD29 pKa = 3.65EE30 pKa = 4.93LIDD33 pKa = 6.09LIFTDD38 pKa = 4.52GTGLTIEE45 pKa = 4.43SCKK48 pKa = 10.32GCCGAGAGLEE58 pKa = 4.3FYY60 pKa = 10.29TYY62 pKa = 10.83INVEE66 pKa = 4.36TEE68 pKa = 4.28GEE70 pKa = 4.2TTLYY74 pKa = 10.82VII76 pKa = 5.34

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3I056|A0A6M3I056_9CAUD Terminase large subunit OS=Pseudanabaena phage PA-SR01 OX=2723892 PE=4 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84TKK5 pKa = 10.42KK6 pKa = 10.14HH7 pKa = 6.48RR8 pKa = 11.84DD9 pKa = 3.02LFFLRR14 pKa = 11.84FLHH17 pKa = 7.08RR18 pKa = 11.84IIKK21 pKa = 10.45AIGSPFTHH29 pKa = 6.5PHH31 pKa = 5.57AARR34 pKa = 11.84WNKK37 pKa = 8.57VRR39 pKa = 11.84CRR41 pKa = 11.84VSRR44 pKa = 11.84NYY46 pKa = 10.68GGSNWGDD53 pKa = 3.2NQYY56 pKa = 11.05VDD58 pKa = 5.34DD59 pKa = 5.36KK60 pKa = 11.92YY61 pKa = 11.85NDD63 pKa = 3.6LPYY66 pKa = 10.65KK67 pKa = 10.35RR68 pKa = 11.84AAKK71 pKa = 9.88RR72 pKa = 11.84SNRR75 pKa = 11.84RR76 pKa = 11.84EE77 pKa = 4.05ALQQAHH83 pKa = 5.3QQLEE87 pKa = 4.39EE88 pKa = 4.04YY89 pKa = 10.2RR90 pKa = 11.84DD91 pKa = 3.85EE92 pKa = 4.16II93 pKa = 5.57

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

165

0

165

41206

32

1918

249.7

27.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.538 ± 0.317

0.837 ± 0.086

5.259 ± 0.126

5.914 ± 0.259

4.385 ± 0.155

6.067 ± 0.165

1.361 ± 0.104

6.608 ± 0.14

6.8 ± 0.314

9.052 ± 0.177

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.31 ± 0.097

6.135 ± 0.191

3.667 ± 0.169

4.147 ± 0.17

4.32 ± 0.174

8.115 ± 0.281

7.264 ± 0.296

5.98 ± 0.151

1.041 ± 0.074

4.198 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski