Amino acid dipepetide frequency for Pseudanabaena phage PA-SR01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.771AlaAla: 6.771 ± 0.579
0.534AlaCys: 0.534 ± 0.15
3.592AlaAsp: 3.592 ± 0.281
4.563AlaGlu: 4.563 ± 0.433
2.645AlaPhe: 2.645 ± 0.345
4.563AlaGly: 4.563 ± 0.453
0.631AlaHis: 0.631 ± 0.143
5.606AlaIle: 5.606 ± 0.384
5.703AlaLys: 5.703 ± 0.538
6.48AlaLeu: 6.48 ± 0.404
0.898AlaMet: 0.898 ± 0.146
5.194AlaAsn: 5.194 ± 0.476
2.791AlaPro: 2.791 ± 0.295
3.228AlaGln: 3.228 ± 0.37
2.961AlaArg: 2.961 ± 0.273
6.019AlaSer: 6.019 ± 0.529
5.8AlaThr: 5.8 ± 0.514
4.271AlaVal: 4.271 ± 0.345
0.582AlaTrp: 0.582 ± 0.141
2.548AlaTyr: 2.548 ± 0.231
0.0AlaXaa: 0.0 ± 0.0
Cys
0.558CysAla: 0.558 ± 0.125
0.073CysCys: 0.073 ± 0.04
0.364CysAsp: 0.364 ± 0.123
0.558CysGlu: 0.558 ± 0.131
0.315CysPhe: 0.315 ± 0.086
0.534CysGly: 0.534 ± 0.14
0.218CysHis: 0.218 ± 0.094
0.485CysIle: 0.485 ± 0.115
0.68CysLys: 0.68 ± 0.176
0.849CysLeu: 0.849 ± 0.167
0.17CysMet: 0.17 ± 0.063
0.388CysAsn: 0.388 ± 0.112
0.582CysPro: 0.582 ± 0.169
0.267CysGln: 0.267 ± 0.089
0.437CysArg: 0.437 ± 0.124
0.582CysSer: 0.582 ± 0.151
0.34CysThr: 0.34 ± 0.087
0.291CysVal: 0.291 ± 0.093
0.121CysTrp: 0.121 ± 0.064
0.558CysTyr: 0.558 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
3.616AspAla: 3.616 ± 0.416
0.558AspCys: 0.558 ± 0.15
2.451AspAsp: 2.451 ± 0.268
3.325AspGlu: 3.325 ± 0.274
3.349AspPhe: 3.349 ± 0.257
3.203AspGly: 3.203 ± 0.244
0.315AspHis: 0.315 ± 0.096
4.417AspIle: 4.417 ± 0.323
3.155AspLys: 3.155 ± 0.346
5.169AspLeu: 5.169 ± 0.411
0.485AspMet: 0.485 ± 0.116
3.179AspAsn: 3.179 ± 0.263
1.675AspPro: 1.675 ± 0.217
1.213AspGln: 1.213 ± 0.175
1.675AspArg: 1.675 ± 0.229
3.907AspSer: 3.907 ± 0.267
3.592AspThr: 3.592 ± 0.295
3.446AspVal: 3.446 ± 0.325
1.189AspTrp: 1.189 ± 0.171
2.67AspTyr: 2.67 ± 0.253
0.0AspXaa: 0.0 ± 0.0
Glu
4.66GluAla: 4.66 ± 0.48
0.437GluCys: 0.437 ± 0.136
3.616GluAsp: 3.616 ± 0.383
4.101GluGlu: 4.101 ± 0.463
3.058GluPhe: 3.058 ± 0.351
3.131GluGly: 3.131 ± 0.298
0.752GluHis: 0.752 ± 0.166
4.077GluIle: 4.077 ± 0.276
4.83GluLys: 4.83 ± 0.443
6.043GluLeu: 6.043 ± 0.413
0.922GluMet: 0.922 ± 0.175
3.058GluAsn: 3.058 ± 0.299
1.602GluPro: 1.602 ± 0.22
2.306GluGln: 2.306 ± 0.27
2.888GluArg: 2.888 ± 0.339
3.932GluSer: 3.932 ± 0.324
3.301GluThr: 3.301 ± 0.316
3.325GluVal: 3.325 ± 0.282
0.534GluTrp: 0.534 ± 0.156
2.573GluTyr: 2.573 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
3.568PheAla: 3.568 ± 0.315
0.291PheCys: 0.291 ± 0.098
2.694PheAsp: 2.694 ± 0.262
2.427PheGlu: 2.427 ± 0.264
1.796PhePhe: 1.796 ± 0.231
3.325PheGly: 3.325 ± 0.317
0.485PheHis: 0.485 ± 0.099
2.281PheIle: 2.281 ± 0.206
2.257PheLys: 2.257 ± 0.214
3.47PheLeu: 3.47 ± 0.328
0.777PheMet: 0.777 ± 0.14
2.33PheAsn: 2.33 ± 0.364
2.014PhePro: 2.014 ± 0.209
1.165PheGln: 1.165 ± 0.195
1.966PheArg: 1.966 ± 0.221
4.805PheSer: 4.805 ± 0.479
3.495PheThr: 3.495 ± 0.346
2.718PheVal: 2.718 ± 0.246
0.34PheTrp: 0.34 ± 0.101
1.65PheTyr: 1.65 ± 0.258
0.0PheXaa: 0.0 ± 0.0
Gly
4.781GlyAla: 4.781 ± 0.532
0.485GlyCys: 0.485 ± 0.151
3.009GlyAsp: 3.009 ± 0.311
3.301GlyGlu: 3.301 ± 0.267
2.645GlyPhe: 2.645 ± 0.26
3.568GlyGly: 3.568 ± 0.337
0.631GlyHis: 0.631 ± 0.141
3.907GlyIle: 3.907 ± 0.34
4.975GlyLys: 4.975 ± 0.436
6.019GlyLeu: 6.019 ± 0.368
0.946GlyMet: 0.946 ± 0.192
4.15GlyAsn: 4.15 ± 0.324
0.0GlyPro: 0.0 ± 0.0
2.427GlyGln: 2.427 ± 0.241
3.131GlyArg: 3.131 ± 0.324
4.611GlySer: 4.611 ± 0.42
4.32GlyThr: 4.32 ± 0.343
4.441GlyVal: 4.441 ± 0.297
0.777GlyTrp: 0.777 ± 0.133
2.548GlyTyr: 2.548 ± 0.263
0.0GlyXaa: 0.0 ± 0.0
His
0.558HisAla: 0.558 ± 0.126
0.243HisCys: 0.243 ± 0.072
0.534HisAsp: 0.534 ± 0.106
0.655HisGlu: 0.655 ± 0.146
1.116HisPhe: 1.116 ± 0.199
0.582HisGly: 0.582 ± 0.229
0.267HisHis: 0.267 ± 0.092
0.946HisIle: 0.946 ± 0.137
1.044HisLys: 1.044 ± 0.182
1.432HisLeu: 1.432 ± 0.221
0.121HisMet: 0.121 ± 0.054
0.752HisAsn: 0.752 ± 0.163
0.752HisPro: 0.752 ± 0.131
0.68HisGln: 0.68 ± 0.151
0.655HisArg: 0.655 ± 0.128
1.092HisSer: 1.092 ± 0.184
0.898HisThr: 0.898 ± 0.17
0.51HisVal: 0.51 ± 0.131
0.243HisTrp: 0.243 ± 0.079
0.534HisTyr: 0.534 ± 0.129
0.0HisXaa: 0.0 ± 0.0
Ile
5.558IleAla: 5.558 ± 0.385
0.558IleCys: 0.558 ± 0.122
4.271IleAsp: 4.271 ± 0.437
4.417IleGlu: 4.417 ± 0.353
2.403IlePhe: 2.403 ± 0.219
4.344IleGly: 4.344 ± 0.391
1.044IleHis: 1.044 ± 0.209
3.422IleIle: 3.422 ± 0.232
4.368IleLys: 4.368 ± 0.426
5.121IleLeu: 5.121 ± 0.37
0.68IleMet: 0.68 ± 0.147
3.81IleAsn: 3.81 ± 0.319
3.228IlePro: 3.228 ± 0.285
2.354IleGln: 2.354 ± 0.235
2.475IleArg: 2.475 ± 0.296
5.97IleSer: 5.97 ± 0.432
5.169IleThr: 5.169 ± 0.467
3.64IleVal: 3.64 ± 0.245
0.413IleTrp: 0.413 ± 0.107
2.839IleTyr: 2.839 ± 0.265
0.0IleXaa: 0.0 ± 0.0
Lys
5.096LysAla: 5.096 ± 0.432
0.437LysCys: 0.437 ± 0.106
3.713LysAsp: 3.713 ± 0.356
4.296LysGlu: 4.296 ± 0.467
2.839LysPhe: 2.839 ± 0.325
3.81LysGly: 3.81 ± 0.32
1.116LysHis: 1.116 ± 0.232
4.83LysIle: 4.83 ± 0.419
4.708LysLys: 4.708 ± 0.511
6.48LysLeu: 6.48 ± 0.43
0.946LysMet: 0.946 ± 0.152
3.786LysAsn: 3.786 ± 0.411
2.597LysPro: 2.597 ± 0.283
3.082LysGln: 3.082 ± 0.297
3.228LysArg: 3.228 ± 0.353
4.708LysSer: 4.708 ± 0.47
4.199LysThr: 4.199 ± 0.33
4.199LysVal: 4.199 ± 0.311
0.825LysTrp: 0.825 ± 0.128
3.106LysTyr: 3.106 ± 0.338
0.0LysXaa: 0.0 ± 0.0
Leu
7.111LeuAla: 7.111 ± 0.456
0.582LeuCys: 0.582 ± 0.129
6.067LeuAsp: 6.067 ± 0.42
5.096LeuGlu: 5.096 ± 0.373
3.543LeuPhe: 3.543 ± 0.295
6.116LeuGly: 6.116 ± 0.43
1.359LeuHis: 1.359 ± 0.229
4.49LeuIle: 4.49 ± 0.341
5.825LeuLys: 5.825 ± 0.415
7.62LeuLeu: 7.62 ± 0.482
1.359LeuMet: 1.359 ± 0.229
5.873LeuAsn: 5.873 ± 0.455
4.247LeuPro: 4.247 ± 0.34
4.514LeuGln: 4.514 ± 0.381
4.101LeuArg: 4.101 ± 0.328
7.305LeuSer: 7.305 ± 0.452
6.868LeuThr: 6.868 ± 0.559
4.878LeuVal: 4.878 ± 0.363
0.461LeuTrp: 0.461 ± 0.098
3.106LeuTyr: 3.106 ± 0.319
0.0LeuXaa: 0.0 ± 0.0
Met
1.189MetAla: 1.189 ± 0.183
0.097MetCys: 0.097 ± 0.051
0.364MetAsp: 0.364 ± 0.089
0.558MetGlu: 0.558 ± 0.121
0.485MetPhe: 0.485 ± 0.095
0.631MetGly: 0.631 ± 0.118
0.194MetHis: 0.194 ± 0.07
0.607MetIle: 0.607 ± 0.126
1.189MetLys: 1.189 ± 0.173
1.068MetLeu: 1.068 ± 0.171
0.218MetMet: 0.218 ± 0.083
0.898MetAsn: 0.898 ± 0.121
0.558MetPro: 0.558 ± 0.149
0.777MetGln: 0.777 ± 0.175
0.582MetArg: 0.582 ± 0.108
1.48MetSer: 1.48 ± 0.244
1.286MetThr: 1.286 ± 0.187
0.413MetVal: 0.413 ± 0.086
0.073MetTrp: 0.073 ± 0.039
0.437MetTyr: 0.437 ± 0.097
0.0MetXaa: 0.0 ± 0.0
Asn
4.029AsnAla: 4.029 ± 0.361
0.777AsnCys: 0.777 ± 0.151
2.475AsnAsp: 2.475 ± 0.232
3.422AsnGlu: 3.422 ± 0.269
3.106AsnPhe: 3.106 ± 0.339
4.053AsnGly: 4.053 ± 0.36
0.752AsnHis: 0.752 ± 0.152
4.781AsnIle: 4.781 ± 0.508
3.616AsnLys: 3.616 ± 0.376
5.946AsnLeu: 5.946 ± 0.421
0.849AsnMet: 0.849 ± 0.153
3.373AsnAsn: 3.373 ± 0.299
2.864AsnPro: 2.864 ± 0.322
2.378AsnGln: 2.378 ± 0.337
2.354AsnArg: 2.354 ± 0.203
5.194AsnSer: 5.194 ± 0.504
4.66AsnThr: 4.66 ± 0.451
2.912AsnVal: 2.912 ± 0.297
0.874AsnTrp: 0.874 ± 0.134
2.937AsnTyr: 2.937 ± 0.255
0.0AsnXaa: 0.0 ± 0.0
Pro
2.281ProAla: 2.281 ± 0.268
0.315ProCys: 0.315 ± 0.085
2.5ProAsp: 2.5 ± 0.271
2.306ProGlu: 2.306 ± 0.23
1.286ProPhe: 1.286 ± 0.21
0.0ProGly: 0.0 ± 0.0
0.825ProHis: 0.825 ± 0.154
3.009ProIle: 3.009 ± 0.288
2.087ProLys: 2.087 ± 0.218
3.009ProLeu: 3.009 ± 0.265
0.413ProMet: 0.413 ± 0.095
2.937ProAsn: 2.937 ± 0.281
1.48ProPro: 1.48 ± 0.193
1.772ProGln: 1.772 ± 0.195
1.553ProArg: 1.553 ± 0.218
3.301ProSer: 3.301 ± 0.386
3.398ProThr: 3.398 ± 0.352
2.378ProVal: 2.378 ± 0.255
0.194ProTrp: 0.194 ± 0.064
1.626ProTyr: 1.626 ± 0.202
0.0ProXaa: 0.0 ± 0.0
Gln
3.228GlnAla: 3.228 ± 0.369
0.364GlnCys: 0.364 ± 0.097
1.844GlnAsp: 1.844 ± 0.179
2.427GlnGlu: 2.427 ± 0.242
1.577GlnPhe: 1.577 ± 0.205
1.772GlnGly: 1.772 ± 0.203
0.631GlnHis: 0.631 ± 0.134
2.961GlnIle: 2.961 ± 0.297
3.058GlnLys: 3.058 ± 0.333
4.247GlnLeu: 4.247 ± 0.352
0.582GlnMet: 0.582 ± 0.121
2.427GlnAsn: 2.427 ± 0.23
1.456GlnPro: 1.456 ± 0.19
2.524GlnGln: 2.524 ± 0.334
1.48GlnArg: 1.48 ± 0.219
2.961GlnSer: 2.961 ± 0.235
2.597GlnThr: 2.597 ± 0.252
2.961GlnVal: 2.961 ± 0.291
0.388GlnTrp: 0.388 ± 0.091
1.99GlnTyr: 1.99 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
2.937ArgAla: 2.937 ± 0.281
0.388ArgCys: 0.388 ± 0.114
2.014ArgAsp: 2.014 ± 0.252
2.718ArgGlu: 2.718 ± 0.326
2.014ArgPhe: 2.014 ± 0.229
2.33ArgGly: 2.33 ± 0.286
0.68ArgHis: 0.68 ± 0.138
3.058ArgIle: 3.058 ± 0.22
3.665ArgLys: 3.665 ± 0.44
4.514ArgLeu: 4.514 ± 0.352
0.364ArgMet: 0.364 ± 0.098
2.306ArgAsn: 2.306 ± 0.244
1.335ArgPro: 1.335 ± 0.174
2.136ArgGln: 2.136 ± 0.295
2.184ArgArg: 2.184 ± 0.29
2.742ArgSer: 2.742 ± 0.223
2.378ArgThr: 2.378 ± 0.238
3.155ArgVal: 3.155 ± 0.308
0.51ArgTrp: 0.51 ± 0.126
1.772ArgTyr: 1.772 ± 0.285
0.0ArgXaa: 0.0 ± 0.0
Ser
6.237SerAla: 6.237 ± 0.539
0.728SerCys: 0.728 ± 0.143
3.713SerAsp: 3.713 ± 0.267
4.393SerGlu: 4.393 ± 0.412
4.174SerPhe: 4.174 ± 0.331
6.189SerGly: 6.189 ± 0.47
0.995SerHis: 0.995 ± 0.176
5.679SerIle: 5.679 ± 0.453
4.854SerLys: 4.854 ± 0.426
7.038SerLeu: 7.038 ± 0.428
1.141SerMet: 1.141 ± 0.16
5.776SerAsn: 5.776 ± 0.597
2.742SerPro: 2.742 ± 0.262
2.937SerGln: 2.937 ± 0.279
3.009SerArg: 3.009 ± 0.28
6.674SerSer: 6.674 ± 0.882
5.509SerThr: 5.509 ± 0.535
5.266SerVal: 5.266 ± 0.461
0.777SerTrp: 0.777 ± 0.149
3.325SerTyr: 3.325 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
5.63ThrAla: 5.63 ± 0.4
0.51ThrCys: 0.51 ± 0.109
3.495ThrAsp: 3.495 ± 0.377
3.81ThrGlu: 3.81 ± 0.316
2.937ThrPhe: 2.937 ± 0.325
5.048ThrGly: 5.048 ± 0.441
0.922ThrHis: 0.922 ± 0.166
4.975ThrIle: 4.975 ± 0.455
4.417ThrLys: 4.417 ± 0.38
6.625ThrLeu: 6.625 ± 0.571
0.631ThrMet: 0.631 ± 0.129
4.708ThrAsn: 4.708 ± 0.489
3.155ThrPro: 3.155 ± 0.338
2.985ThrGln: 2.985 ± 0.249
2.621ThrArg: 2.621 ± 0.262
5.582ThrSer: 5.582 ± 0.577
6.334ThrThr: 6.334 ± 0.851
4.223ThrVal: 4.223 ± 0.31
0.898ThrTrp: 0.898 ± 0.161
3.106ThrTyr: 3.106 ± 0.32
0.0ThrXaa: 0.0 ± 0.0
Val
4.684ValAla: 4.684 ± 0.358
0.461ValCys: 0.461 ± 0.123
3.058ValAsp: 3.058 ± 0.271
3.81ValGlu: 3.81 ± 0.283
2.063ValPhe: 2.063 ± 0.261
4.296ValGly: 4.296 ± 0.461
1.068ValHis: 1.068 ± 0.248
3.349ValIle: 3.349 ± 0.312
3.956ValLys: 3.956 ± 0.323
4.587ValLeu: 4.587 ± 0.313
0.752ValMet: 0.752 ± 0.134
3.422ValAsn: 3.422 ± 0.316
2.014ValPro: 2.014 ± 0.22
2.524ValGln: 2.524 ± 0.224
3.009ValArg: 3.009 ± 0.331
5.339ValSer: 5.339 ± 0.403
4.611ValThr: 4.611 ± 0.369
3.883ValVal: 3.883 ± 0.294
0.68ValTrp: 0.68 ± 0.14
2.233ValTyr: 2.233 ± 0.256
0.0ValXaa: 0.0 ± 0.0
Trp
0.51TrpAla: 0.51 ± 0.124
0.146TrpCys: 0.146 ± 0.068
0.704TrpAsp: 0.704 ± 0.148
0.51TrpGlu: 0.51 ± 0.108
0.364TrpPhe: 0.364 ± 0.104
0.825TrpGly: 0.825 ± 0.139
0.17TrpHis: 0.17 ± 0.062
0.68TrpIle: 0.68 ± 0.145
0.631TrpLys: 0.631 ± 0.139
0.752TrpLeu: 0.752 ± 0.131
0.194TrpMet: 0.194 ± 0.061
0.51TrpAsn: 0.51 ± 0.105
0.0TrpPro: 0.0 ± 0.0
0.485TrpGln: 0.485 ± 0.112
0.752TrpArg: 0.752 ± 0.143
0.946TrpSer: 0.946 ± 0.149
0.849TrpThr: 0.849 ± 0.16
0.825TrpVal: 0.825 ± 0.146
0.073TrpTrp: 0.073 ± 0.042
0.485TrpTyr: 0.485 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.378TyrAla: 2.378 ± 0.294
0.388TyrCys: 0.388 ± 0.109
2.111TyrAsp: 2.111 ± 0.211
2.451TyrGlu: 2.451 ± 0.26
2.136TyrPhe: 2.136 ± 0.236
2.354TyrGly: 2.354 ± 0.243
0.558TyrHis: 0.558 ± 0.114
2.524TyrIle: 2.524 ± 0.233
2.985TyrLys: 2.985 ± 0.328
4.053TyrLeu: 4.053 ± 0.352
0.631TyrMet: 0.631 ± 0.116
2.475TyrAsn: 2.475 ± 0.232
1.577TyrPro: 1.577 ± 0.217
1.723TyrGln: 1.723 ± 0.199
2.136TyrArg: 2.136 ± 0.247
4.004TyrSer: 4.004 ± 0.407
3.034TyrThr: 3.034 ± 0.364
2.063TyrVal: 2.063 ± 0.247
0.461TyrTrp: 0.461 ± 0.101
1.942TyrTyr: 1.942 ± 0.305
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 165 proteins (41206 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski