Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) (Verticillium wilt)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Plectosphaerellaceae; Verticillium; Verticillium dahliae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10530 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2X2Y2|G2X2Y2_VERDV Uncharacterized protein OS=Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) OX=498257 GN=VDAG_04176 PE=4 SV=1
MM1 pKa = 7.12PQALRR6 pKa = 11.84VLPVALLAARR16 pKa = 11.84LPDD19 pKa = 3.8LSFLKK24 pKa = 10.59SLPGLVNEE32 pKa = 5.55AGPGDD37 pKa = 4.2PGAGAGTGPGTIIPDD52 pKa = 3.75AVNSIPGVIGGALGNGPASGIVPGLANALPGLVNDD87 pKa = 4.98TLAGGPGSGPGTLLPDD103 pKa = 3.65NVNNIPSVIGGALGNSTTSAGLIPGLIGSLPGIVNDD139 pKa = 4.97TIGGAAPGPLIPDD152 pKa = 4.24AVNNMPSIIGGAIGSVPGNGSAIVPGLLNDD182 pKa = 4.72LPGLVNGVFQNQTGAGTVLPDD203 pKa = 5.83AINTLPGVIGGALSGDD219 pKa = 3.88NPGSAIPTVIDD230 pKa = 3.95NLPGLINGSVGQTAPDD246 pKa = 3.18SGGAILPGPLNEE258 pKa = 4.55LPGIIGGAVNGSTPSPEE275 pKa = 3.84GVVPGLVNNLPAIVAGASGNQSGAGSILPGPLNEE309 pKa = 5.72LPGLLNSTLGAGGPVAGTLVPGIVNGLPDD338 pKa = 4.64LVNGALNGTNGGSSAGPGGLPGTLLPGGLNEE369 pKa = 5.2LPGNLTAGLPNGTTAGTLLPSIIGGLPGAVNSVVNGTAGTLIPGVVNQVPGIIKK423 pKa = 10.2GAVDD427 pKa = 3.85SGPNIGPLIPGIVGSFPAIVNGTVSGGDD455 pKa = 3.29QPQQDD460 pKa = 5.22LEE462 pKa = 4.31TLIPGIIEE470 pKa = 4.43TIPTCVEE477 pKa = 3.82GALGNVPNVNPADD490 pKa = 4.3IIPIVIGRR498 pKa = 11.84LPDD501 pKa = 4.5IISQLPNVTTPGQVLPGPLNQLPGIISGAISDD533 pKa = 4.12AAGVQPGVIAPGLVATLPSVLEE555 pKa = 3.98GLVNNLPNTGGAVRR569 pKa = 11.84PTIPGLSEE577 pKa = 4.22SLPGLCEE584 pKa = 3.99DD585 pKa = 4.76ALNSGSGALIPEE597 pKa = 4.55VVNTLPGVIGGAISNGQLPGSAISLPDD624 pKa = 3.78LVNNLPGLINEE635 pKa = 4.88ALNKK639 pKa = 9.77GQEE642 pKa = 3.98EE643 pKa = 5.19CIPGPVNNLPTTIGNVISNGSLPIPNLPISDD674 pKa = 4.65IINNLPEE681 pKa = 5.39LINGAIQTGAGTLIPDD697 pKa = 4.44RR698 pKa = 11.84LNNLPADD705 pKa = 3.53IGVSLNATVGLPVPEE720 pKa = 4.3ILANLPSIINNAVSSGNPVAIPEE743 pKa = 4.45VINNLPATIGTAVGGATNLIPQIIGNLSALVNEE776 pKa = 5.86AINSGASTVLPPVINNLPVTIGGALNGTTLPPAIISNLPALINGAIGNGSEE827 pKa = 4.36TAGGAATLLPEE838 pKa = 5.94AINTLPAVIGGSLGGLTGNAASLVPAIVANLPGLVTGALQSGAGTLVPPSVNNLPTTIGEE898 pKa = 4.4SLGNLTGNAGALVPEE913 pKa = 5.57IINNLPGLIAGGLVGGTGTLLPPAVNNLPAVIGGALGNVTGAAEE957 pKa = 4.11SLVPQIIANLPGLITGAIGSNSTTLIPDD985 pKa = 3.73VVNTLPVVIGGALTNSSIGFNPSIINNLPSLVNNAISGGNTTIIPPQVNNIPAVISGAIANGTTAGDD1052 pKa = 4.87LINNLIGNGTATAGGLLPILSGTLNGNVSIGGSSQGVLPGLIANVSVDD1100 pKa = 3.44ALGPNIPTLINGLIGTNGTLNAIAGGLLGNGTAGLTIPDD1139 pKa = 3.7ILGGIAAGTGNSSLPGLVGEE1159 pKa = 4.61ILGNGTGGGIIGGGGIGGAGAGLGVGVTVGGGLGAGGLLQNITTGLIGGGSGGASIPGVLPGIVAAGPRR1228 pKa = 11.84ANTTVPSVEE1237 pKa = 4.05VVLDD1241 pKa = 3.72NLGLSNLTASLPALVDD1257 pKa = 4.19SIIGSANLTGKK1268 pKa = 10.38NITGPAASLIDD1279 pKa = 3.81GLLGPGASAGSNVTLGPASGPGAGLIPNLLDD1310 pKa = 3.65NVATGNVTQIIPDD1323 pKa = 4.25LVNGLLGNGTANSGGLIPDD1342 pKa = 4.65LLQNASAGALPAVIPNIVGGLLGGATVGGAAGDD1375 pKa = 4.01LNNPGVGVNGSTNVNGTAGIGGILPTLVGSLNSGNLSNIVPGILTGLLGNGTASSGGLIPAVVVNINNGTTGANANANANATADD1459 pKa = 3.72VVNTGIIDD1467 pKa = 3.91GLLGAGSGGALLNTGGLLSGTRR1489 pKa = 11.84NLLTDD1494 pKa = 4.01SLNCGAIGIVCQGIQICRR1512 pKa = 11.84AGVCINSSGPSGTSTIPAAGTPTSPAAGISTTVVPGQGVVDD1553 pKa = 4.9LLNDD1557 pKa = 3.6SLNCGRR1563 pKa = 11.84LLNVCLFGQICSNGQCIVAGATTTAPPAAAQPTTTALPATTTVAGGNIINFLTDD1617 pKa = 3.23NLNCGRR1623 pKa = 11.84LLNVCLFGQVCRR1635 pKa = 11.84EE1636 pKa = 4.16GQCVSAAQATTAAIAPATTTIAVQVTTTAAGGQLTTVQVPTTVVAAAPTAAGADD1690 pKa = 3.45AAVGIVDD1697 pKa = 4.88HH1698 pKa = 7.07LNDD1701 pKa = 3.71SLNCGRR1707 pKa = 11.84LFNIYY1712 pKa = 10.05ILGQTCRR1719 pKa = 11.84NGVCVASGAAVPTTTAGTTAPTTTARR1745 pKa = 11.84ATTTAPVVGTTTTAAAAAPTTTVAAAPTTTAGGAFGVVDD1784 pKa = 5.21LLNDD1788 pKa = 3.76ALNCGRR1794 pKa = 11.84LLNICLFGQVCRR1806 pKa = 11.84SGVCVAAGAAVPTTTAPATTAPTTTLAATTAVVTTTLLTATRR1848 pKa = 11.84AATTTTVAGVPTTIAGTTTIAATITAAVAPTTTTGAATATTTGGTLAVSLDD1899 pKa = 3.59LHH1901 pKa = 5.92TDD1903 pKa = 3.29RR1904 pKa = 11.84LNCGRR1909 pKa = 11.84IGNICLGSQTCSSGLCISVGGAVVGGNTIATTATAATTTTTSTTAPAAAAPTTTAGTTTRR1969 pKa = 11.84AATTTTAAAPTTTAAAPTTTAAAPTTTGAQTNVAIAVSTTPTTAPAAVPTTTARR2023 pKa = 11.84VTTTVPTTPTVQAVPTTPTVQAVPTTPTVQAAPTTVRR2060 pKa = 11.84AATTTAAAPTTAPQAATTTQRR2081 pKa = 11.84AATTTAPSTGSSGSTGSSGSGASCGGFFNLIPCIFPLTCVNGRR2124 pKa = 11.84CQLLGVIGG2132 pKa = 3.74

Molecular weight: 201.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2XET4|G2XET4_VERDV Multiprotein-bridging factor 1 OS=Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) OX=498257 GN=VDAG_08669 PE=3 SV=1
MM1 pKa = 7.94PILRR5 pKa = 11.84SSQPRR10 pKa = 11.84RR11 pKa = 11.84TRR13 pKa = 11.84HH14 pKa = 4.15TTTKK18 pKa = 9.78TSRR21 pKa = 11.84SNGGLFSRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84QPARR35 pKa = 11.84TTHH38 pKa = 5.14TTTTTTTTHH47 pKa = 6.21RR48 pKa = 11.84QHH50 pKa = 7.12AGATAPRR57 pKa = 11.84GGFFSRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84GPVVHH70 pKa = 6.33HH71 pKa = 5.68QQRR74 pKa = 11.84KK75 pKa = 8.1PSMGDD80 pKa = 3.22KK81 pKa = 10.49ISGAMLRR88 pKa = 11.84LKK90 pKa = 10.8GSLTRR95 pKa = 11.84RR96 pKa = 11.84PGQKK100 pKa = 9.9AAGTRR105 pKa = 11.84RR106 pKa = 11.84MNGTDD111 pKa = 2.82GRR113 pKa = 11.84GSHH116 pKa = 5.61RR117 pKa = 11.84TRR119 pKa = 11.84RR120 pKa = 11.84WW121 pKa = 2.82

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10530

0

10530

4983248

29

7054

473.2

52.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.716 ± 0.024

1.215 ± 0.013

5.91 ± 0.02

5.95 ± 0.027

3.565 ± 0.015

7.196 ± 0.027

2.442 ± 0.011

4.412 ± 0.014

4.505 ± 0.024

8.706 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.01

3.4 ± 0.013

6.286 ± 0.025

3.997 ± 0.017

6.396 ± 0.022

7.892 ± 0.025

5.984 ± 0.018

6.263 ± 0.017

1.455 ± 0.009

2.536 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski