Rhizobacter sp. Root404

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Rhizobacter; unclassified Rhizobacter

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7AGQ4|A0A0Q7AGQ4_9BURK Guanylate cyclase domain-containing protein OS=Rhizobacter sp. Root404 OX=1736528 GN=ASC76_22885 PE=4 SV=1
MM1 pKa = 6.2TTEE4 pKa = 4.08AVALASYY11 pKa = 9.52SVKK14 pKa = 9.21ITSLPVLGDD23 pKa = 3.34LMYY26 pKa = 10.94FNGVSLVPVALNSTYY41 pKa = 10.43TYY43 pKa = 11.47ADD45 pKa = 3.81IIAGKK50 pKa = 9.47LQFVPDD56 pKa = 4.34ANQSGIDD63 pKa = 3.82GYY65 pKa = 11.43GAAGTGNKK73 pKa = 10.08LNDD76 pKa = 3.34YY77 pKa = 10.94ASFNFEE83 pKa = 4.28VIANGINSNPATVVIDD99 pKa = 3.69IAPVADD105 pKa = 4.7APTLSLTTASFLRR118 pKa = 11.84TSSLDD123 pKa = 3.23EE124 pKa = 4.06LTAMSGPNAWTRR136 pKa = 11.84YY137 pKa = 6.85ATDD140 pKa = 3.47MNGGAWRR147 pKa = 11.84TSNSGGTIEE156 pKa = 4.72VYY158 pKa = 11.14ADD160 pKa = 3.74GEE162 pKa = 4.33YY163 pKa = 10.63LQNGGSNRR171 pKa = 11.84VIEE174 pKa = 4.51LEE176 pKa = 4.32SNTGAAADD184 pKa = 5.07LYY186 pKa = 10.93TDD188 pKa = 3.72VATKK192 pKa = 10.47AGQVYY197 pKa = 10.23HH198 pKa = 6.93LSLDD202 pKa = 3.47FAEE205 pKa = 4.63RR206 pKa = 11.84LNNQSMASQGLIPAYY221 pKa = 9.02GTAQIDD227 pKa = 4.62VYY229 pKa = 10.45WGGVKK234 pKa = 9.93VATLNTDD241 pKa = 3.17TSTWTHH247 pKa = 6.38FEE249 pKa = 4.15LDD251 pKa = 3.46LAATTTGTTRR261 pKa = 11.84LTFQATDD268 pKa = 3.55SNSLGGVMDD277 pKa = 4.27NLSLQLSQNTTMRR290 pKa = 11.84NTTTQLPQPTIALVDD305 pKa = 3.68TDD307 pKa = 3.82GSEE310 pKa = 4.19TLALTVTGVPIGATISDD327 pKa = 3.89GTRR330 pKa = 11.84SFTATAGNQVATITGWTYY348 pKa = 11.19SALTVTPPAGYY359 pKa = 8.53TGSFTLNYY367 pKa = 7.78TATSTEE373 pKa = 3.93AVGGSTATTITPVTVNVIAPNTGPVATNDD402 pKa = 3.48TVTGIRR408 pKa = 11.84DD409 pKa = 3.67VVVHH413 pKa = 6.35GNVLTNDD420 pKa = 3.11TDD422 pKa = 4.31ADD424 pKa = 4.18GNTLYY429 pKa = 9.77VTGFTVNGTNYY440 pKa = 8.88ATYY443 pKa = 10.69DD444 pKa = 3.72SVGVTDD450 pKa = 3.64NTTDD454 pKa = 3.02NVTIAGVGYY463 pKa = 10.16FRR465 pKa = 11.84MEE467 pKa = 4.02ADD469 pKa = 3.08GSYY472 pKa = 10.5QFTPLAGYY480 pKa = 8.04TGTAPAITYY489 pKa = 7.16TTADD493 pKa = 3.17EE494 pKa = 4.57HH495 pKa = 7.06GGYY498 pKa = 7.91STATLTLTEE507 pKa = 4.42TTTGGIIGTSSAEE520 pKa = 4.27TLNGTAGADD529 pKa = 3.65VISASGGIDD538 pKa = 3.26TLNGLGGNDD547 pKa = 3.72VLDD550 pKa = 4.54GGAGADD556 pKa = 3.62VLVGGAGSDD565 pKa = 3.57VLYY568 pKa = 11.08GRR570 pKa = 11.84AGNDD574 pKa = 3.09TLTGGTGTGTDD585 pKa = 3.32TASNTFAWAFGDD597 pKa = 3.54QGTTSTLAVDD607 pKa = 4.91TITDD611 pKa = 3.89FNKK614 pKa = 10.34AAASSGGDD622 pKa = 3.43VLDD625 pKa = 5.63LRR627 pKa = 11.84DD628 pKa = 3.92LLIGEE633 pKa = 4.36YY634 pKa = 10.64HH635 pKa = 7.11NAFQPNGNLTDD646 pKa = 4.07FLHH649 pKa = 6.31FTTSAGTTTIEE660 pKa = 4.42VKK662 pKa = 10.31SHH664 pKa = 5.46GVGTSSADD672 pKa = 3.42EE673 pKa = 4.76KK674 pKa = 11.01IVLTGVDD681 pKa = 4.11LTNGGALNTDD691 pKa = 3.17QLIIQDD697 pKa = 4.66LLSKK701 pKa = 10.89GKK703 pKa = 10.45LVVDD707 pKa = 4.31

Molecular weight: 72.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7AKX4|A0A0Q7AKX4_9BURK Nicotinate phosphoribosyltransferase OS=Rhizobacter sp. Root404 OX=1736528 GN=ASC76_06030 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.71QASKK9 pKa = 9.04VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4707

0

4707

1567174

44

10585

332.9

35.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.683 ± 0.06

0.86 ± 0.012

5.454 ± 0.039

4.997 ± 0.037

3.507 ± 0.024

8.45 ± 0.048

2.196 ± 0.02

4.253 ± 0.023

3.014 ± 0.034

10.683 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.022

2.462 ± 0.026

5.348 ± 0.029

3.533 ± 0.02

7.205 ± 0.046

5.133 ± 0.03

5.407 ± 0.041

7.893 ± 0.029

1.456 ± 0.017

2.083 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski