Amino acid dipepetide frequency for Rhizobacter sp. Root404

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.418AlaAla: 20.418 ± 0.17	1.233AlaCys: 1.233 ± 0.03	7.131AlaAsp: 7.131 ± 0.08	6.676AlaGlu: 6.676 ± 0.082	4.345AlaPhe: 4.345 ± 0.053	11.288AlaGly: 11.288 ± 0.117	2.797AlaHis: 2.797 ± 0.048	5.554AlaIle: 5.554 ± 0.084	3.915AlaLys: 3.915 ± 0.067	16.116AlaLeu: 16.116 ± 0.121	3.571AlaMet: 3.571 ± 0.053	2.996AlaAsn: 2.996 ± 0.049	7.078AlaPro: 7.078 ± 0.09	5.631AlaGln: 5.631 ± 0.074	9.865AlaArg: 9.865 ± 0.111	7.041AlaSer: 7.041 ± 0.085	7.048AlaThr: 7.048 ± 0.083	9.601AlaVal: 9.601 ± 0.09	2.071AlaTrp: 2.071 ± 0.04	2.455AlaTyr: 2.455 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.137CysAla: 1.137 ± 0.033	0.096CysCys: 0.096 ± 0.008	0.542CysAsp: 0.542 ± 0.021	0.486CysGlu: 0.486 ± 0.021	0.281CysPhe: 0.281 ± 0.014	0.893CysGly: 0.893 ± 0.026	0.256CysHis: 0.256 ± 0.014	0.38CysIle: 0.38 ± 0.017	0.191CysLys: 0.191 ± 0.011	0.744CysLeu: 0.744 ± 0.023	0.193CysMet: 0.193 ± 0.011	0.205CysAsn: 0.205 ± 0.013	0.432CysPro: 0.432 ± 0.018	0.219CysGln: 0.219 ± 0.012	0.561CysArg: 0.561 ± 0.019	0.496CysSer: 0.496 ± 0.02	0.463CysThr: 0.463 ± 0.02	0.732CysVal: 0.732 ± 0.021	0.115CysTrp: 0.115 ± 0.01	0.175CysTyr: 0.175 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.396AspAla: 8.396 ± 0.089	0.445AspCys: 0.445 ± 0.019	3.129AspAsp: 3.129 ± 0.066	3.158AspGlu: 3.158 ± 0.052	1.932AspPhe: 1.932 ± 0.043	4.969AspGly: 4.969 ± 0.07	1.182AspHis: 1.182 ± 0.029	2.228AspIle: 2.228 ± 0.043	1.639AspLys: 1.639 ± 0.037	5.465AspLeu: 5.465 ± 0.067	1.111AspMet: 1.111 ± 0.027	1.14AspAsn: 1.14 ± 0.032	3.242AspPro: 3.242 ± 0.052	1.512AspGln: 1.512 ± 0.037	3.626AspArg: 3.626 ± 0.051	2.226AspSer: 2.226 ± 0.043	2.651AspThr: 2.651 ± 0.06	4.248AspVal: 4.248 ± 0.062	0.941AspTrp: 0.941 ± 0.026	1.302AspTyr: 1.302 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.553GluAla: 6.553 ± 0.073	0.376GluCys: 0.376 ± 0.017	1.935GluAsp: 1.935 ± 0.038	2.02GluGlu: 2.02 ± 0.042	1.798GluPhe: 1.798 ± 0.04	3.388GluGly: 3.388 ± 0.055	1.343GluHis: 1.343 ± 0.029	2.549GluIle: 2.549 ± 0.043	1.523GluLys: 1.523 ± 0.04	5.896GluLeu: 5.896 ± 0.074	1.251GluMet: 1.251 ± 0.03	1.126GluAsn: 1.126 ± 0.027	2.644GluPro: 2.644 ± 0.048	2.189GluGln: 2.189 ± 0.044	4.916GluArg: 4.916 ± 0.076	2.466GluSer: 2.466 ± 0.041	2.499GluThr: 2.499 ± 0.044	3.89GluVal: 3.89 ± 0.058	0.698GluTrp: 0.698 ± 0.023	0.909GluTyr: 0.909 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.634PheAla: 4.634 ± 0.055	0.344PheCys: 0.344 ± 0.015	2.782PheAsp: 2.782 ± 0.05	2.178PheGlu: 2.178 ± 0.037	1.311PhePhe: 1.311 ± 0.037	3.589PheGly: 3.589 ± 0.055	0.691PheHis: 0.691 ± 0.021	1.417PheIle: 1.417 ± 0.032	1.12PheLys: 1.12 ± 0.03	2.727PheLeu: 2.727 ± 0.048	0.78PheMet: 0.78 ± 0.023	1.079PheAsn: 1.079 ± 0.027	1.441PhePro: 1.441 ± 0.033	0.957PheGln: 0.957 ± 0.027	1.893PheArg: 1.893 ± 0.035	1.902PheSer: 1.902 ± 0.035	1.993PheThr: 1.993 ± 0.039	2.933PheVal: 2.933 ± 0.046	0.509PheTrp: 0.509 ± 0.018	0.79PheTyr: 0.79 ± 0.022	0.001PheXaa: 0.001 ± 0.001
Gly	10.615GlyAla: 10.615 ± 0.118	0.898GlyCys: 0.898 ± 0.024	4.252GlyAsp: 4.252 ± 0.091	4.263GlyGlu: 4.263 ± 0.057	3.288GlyPhe: 3.288 ± 0.046	7.249GlyGly: 7.249 ± 0.121	1.961GlyHis: 1.961 ± 0.036	3.889GlyIle: 3.889 ± 0.058	2.932GlyLys: 2.932 ± 0.059	8.973GlyLeu: 8.973 ± 0.084	2.164GlyMet: 2.164 ± 0.045	2.051GlyAsn: 2.051 ± 0.057	3.293GlyPro: 3.293 ± 0.05	2.956GlyGln: 2.956 ± 0.045	5.718GlyArg: 5.718 ± 0.072	4.643GlySer: 4.643 ± 0.077	4.787GlyThr: 4.787 ± 0.075	6.958GlyVal: 6.958 ± 0.08	1.503GlyTrp: 1.503 ± 0.033	2.108GlyTyr: 2.108 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	3.092HisAla: 3.092 ± 0.057	0.239HisCys: 0.239 ± 0.013	1.279HisAsp: 1.279 ± 0.029	1.116HisGlu: 1.116 ± 0.027	0.862HisPhe: 0.862 ± 0.022	2.153HisGly: 2.153 ± 0.037	0.623HisHis: 0.623 ± 0.023	0.813HisIle: 0.813 ± 0.023	0.491HisLys: 0.491 ± 0.016	2.256HisLeu: 2.256 ± 0.044	0.436HisMet: 0.436 ± 0.018	0.413HisAsn: 0.413 ± 0.015	1.483HisPro: 1.483 ± 0.037	0.655HisGln: 0.655 ± 0.02	1.566HisArg: 1.566 ± 0.035	0.913HisSer: 0.913 ± 0.024	0.96HisThr: 0.96 ± 0.025	1.626HisVal: 1.626 ± 0.031	0.415HisTrp: 0.415 ± 0.016	0.566HisTyr: 0.566 ± 0.021	0.001HisXaa: 0.001 ± 0.001
Ile	6.629IleAla: 6.629 ± 0.073	0.365IleCys: 0.365 ± 0.016	3.34IleAsp: 3.34 ± 0.059	3.079IleGlu: 3.079 ± 0.051	1.208IlePhe: 1.208 ± 0.03	4.571IleGly: 4.571 ± 0.071	0.838IleHis: 0.838 ± 0.025	1.281IleIle: 1.281 ± 0.038	1.311IleLys: 1.311 ± 0.037	2.882IleLeu: 2.882 ± 0.046	0.657IleMet: 0.657 ± 0.02	1.234IleAsn: 1.234 ± 0.033	1.85IlePro: 1.85 ± 0.039	1.078IleGln: 1.078 ± 0.026	2.526IleArg: 2.526 ± 0.041	2.129IleSer: 2.129 ± 0.037	2.393IleThr: 2.393 ± 0.051	3.872IleVal: 3.872 ± 0.054	0.423IleTrp: 0.423 ± 0.017	0.862IleTyr: 0.862 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	3.783LysAla: 3.783 ± 0.07	0.158LysCys: 0.158 ± 0.013	1.475LysAsp: 1.475 ± 0.037	1.297LysGlu: 1.297 ± 0.038	0.954LysPhe: 0.954 ± 0.027	2.271LysGly: 2.271 ± 0.041	0.634LysHis: 0.634 ± 0.02	1.315LysIle: 1.315 ± 0.032	1.269LysLys: 1.269 ± 0.048	3.297LysLeu: 3.297 ± 0.059	0.745LysMet: 0.745 ± 0.024	0.864LysAsn: 0.864 ± 0.023	1.924LysPro: 1.924 ± 0.038	1.072LysGln: 1.072 ± 0.03	2.217LysArg: 2.217 ± 0.039	1.628LysSer: 1.628 ± 0.038	1.785LysThr: 1.785 ± 0.036	2.489LysVal: 2.489 ± 0.044	0.34LysTrp: 0.34 ± 0.016	0.623LysTyr: 0.623 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	15.558LeuAla: 15.558 ± 0.12	0.919LeuCys: 0.919 ± 0.025	6.082LeuAsp: 6.082 ± 0.079	4.843LeuGlu: 4.843 ± 0.066	3.461LeuPhe: 3.461 ± 0.059	8.808LeuGly: 8.808 ± 0.091	2.285LeuHis: 2.285 ± 0.047	4.331LeuIle: 4.331 ± 0.064	3.486LeuLys: 3.486 ± 0.058	11.171LeuLeu: 11.171 ± 0.132	2.51LeuMet: 2.51 ± 0.038	2.702LeuAsn: 2.702 ± 0.041	5.983LeuPro: 5.983 ± 0.063	3.878LeuGln: 3.878 ± 0.061	8.197LeuArg: 8.197 ± 0.094	5.618LeuSer: 5.618 ± 0.069	5.634LeuThr: 5.634 ± 0.078	8.087LeuVal: 8.087 ± 0.082	1.291LeuTrp: 1.291 ± 0.031	1.984LeuTyr: 1.984 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.892MetAla: 2.892 ± 0.051	0.167MetCys: 0.167 ± 0.01	1.071MetAsp: 1.071 ± 0.027	0.904MetGlu: 0.904 ± 0.026	0.715MetPhe: 0.715 ± 0.023	1.716MetGly: 1.716 ± 0.037	0.514MetHis: 0.514 ± 0.016	0.983MetIle: 0.983 ± 0.025	0.981MetLys: 0.981 ± 0.028	2.672MetLeu: 2.672 ± 0.054	0.56MetMet: 0.56 ± 0.021	0.909MetAsn: 0.909 ± 0.024	1.476MetPro: 1.476 ± 0.036	0.957MetGln: 0.957 ± 0.024	1.805MetArg: 1.805 ± 0.037	1.554MetSer: 1.554 ± 0.032	1.707MetThr: 1.707 ± 0.039	1.679MetVal: 1.679 ± 0.033	0.202MetTrp: 0.202 ± 0.011	0.38MetTyr: 0.38 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.351AsnAla: 3.351 ± 0.055	0.243AsnCys: 0.243 ± 0.014	1.36AsnAsp: 1.36 ± 0.043	1.155AsnGlu: 1.155 ± 0.024	0.93AsnPhe: 0.93 ± 0.027	2.182AsnGly: 2.182 ± 0.053	0.443AsnHis: 0.443 ± 0.017	1.041AsnIle: 1.041 ± 0.03	0.71AsnLys: 0.71 ± 0.021	2.579AsnLeu: 2.579 ± 0.045	0.495AsnMet: 0.495 ± 0.02	0.657AsnAsn: 0.657 ± 0.027	1.697AsnPro: 1.697 ± 0.032	0.773AsnGln: 0.773 ± 0.024	1.593AsnArg: 1.593 ± 0.038	1.094AsnSer: 1.094 ± 0.033	1.336AsnThr: 1.336 ± 0.038	1.985AsnVal: 1.985 ± 0.035	0.371AsnTrp: 0.371 ± 0.017	0.629AsnTyr: 0.629 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.796ProAla: 7.796 ± 0.088	0.334ProCys: 0.334 ± 0.013	3.196ProAsp: 3.196 ± 0.046	2.997ProGlu: 2.997 ± 0.052	1.855ProPhe: 1.855 ± 0.035	4.67ProGly: 4.67 ± 0.062	1.086ProHis: 1.086 ± 0.028	2.007ProIle: 2.007 ± 0.042	1.588ProLys: 1.588 ± 0.034	5.326ProLeu: 5.326 ± 0.062	1.358ProMet: 1.358 ± 0.032	1.307ProAsn: 1.307 ± 0.031	2.962ProPro: 2.962 ± 0.061	1.894ProGln: 1.894 ± 0.036	3.222ProArg: 3.222 ± 0.057	2.709ProSer: 2.709 ± 0.044	2.922ProThr: 2.922 ± 0.042	4.389ProVal: 4.389 ± 0.051	0.763ProTrp: 0.763 ± 0.02	1.096ProTyr: 1.096 ± 0.027	0.001ProXaa: 0.001 ± 0.001
Gln	5.087GlnAla: 5.087 ± 0.073	0.255GlnCys: 0.255 ± 0.014	1.295GlnAsp: 1.295 ± 0.033	1.217GlnGlu: 1.217 ± 0.033	1.154GlnPhe: 1.154 ± 0.025	2.66GlnGly: 2.66 ± 0.038	0.793GlnHis: 0.793 ± 0.023	1.667GlnIle: 1.667 ± 0.035	0.88GlnLys: 0.88 ± 0.025	4.017GlnLeu: 4.017 ± 0.053	0.868GlnMet: 0.868 ± 0.022	0.711GlnAsn: 0.711 ± 0.024	2.028GlnPro: 2.028 ± 0.036	1.593GlnGln: 1.593 ± 0.038	3.414GlnArg: 3.414 ± 0.065	1.771GlnSer: 1.771 ± 0.034	1.822GlnThr: 1.822 ± 0.038	2.88GlnVal: 2.88 ± 0.046	0.545GlnTrp: 0.545 ± 0.017	0.669GlnTyr: 0.669 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	9.168ArgAla: 9.168 ± 0.093	0.592ArgCys: 0.592 ± 0.023	4.04ArgAsp: 4.04 ± 0.055	4.165ArgGlu: 4.165 ± 0.07	2.861ArgPhe: 2.861 ± 0.047	5.156ArgGly: 5.156 ± 0.068	1.902ArgHis: 1.902 ± 0.042	3.394ArgIle: 3.394 ± 0.044	1.797ArgLys: 1.797 ± 0.04	8.045ArgLeu: 8.045 ± 0.088	1.946ArgMet: 1.946 ± 0.038	1.629ArgAsn: 1.629 ± 0.031	3.469ArgPro: 3.469 ± 0.054	2.633ArgGln: 2.633 ± 0.046	5.581ArgArg: 5.581 ± 0.082	3.698ArgSer: 3.698 ± 0.053	3.448ArgThr: 3.448 ± 0.046	5.557ArgVal: 5.557 ± 0.069	1.269ArgTrp: 1.269 ± 0.031	1.699ArgTyr: 1.699 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.683SerAla: 6.683 ± 0.072	0.415SerCys: 0.415 ± 0.018	2.502SerAsp: 2.502 ± 0.05	2.238SerGlu: 2.238 ± 0.039	2.055SerPhe: 2.055 ± 0.036	5.153SerGly: 5.153 ± 0.075	1.067SerHis: 1.067 ± 0.025	2.381SerIle: 2.381 ± 0.048	1.427SerLys: 1.427 ± 0.036	5.309SerLeu: 5.309 ± 0.066	1.345SerMet: 1.345 ± 0.031	1.307SerAsn: 1.307 ± 0.034	2.752SerPro: 2.752 ± 0.038	1.536SerGln: 1.536 ± 0.035	3.371SerArg: 3.371 ± 0.05	2.76SerSer: 2.76 ± 0.055	3.078SerThr: 3.078 ± 0.067	4.104SerVal: 4.104 ± 0.064	0.689SerTrp: 0.689 ± 0.02	1.155SerTyr: 1.155 ± 0.029	0.001SerXaa: 0.001 ± 0.001
Thr	6.616ThrAla: 6.616 ± 0.084	0.428ThrCys: 0.428 ± 0.016	2.821ThrAsp: 2.821 ± 0.052	2.425ThrGlu: 2.425 ± 0.042	1.829ThrPhe: 1.829 ± 0.04	4.885ThrGly: 4.885 ± 0.086	1.182ThrHis: 1.182 ± 0.029	2.321ThrIle: 2.321 ± 0.049	1.355ThrLys: 1.355 ± 0.031	6.63ThrLeu: 6.63 ± 0.067	1.202ThrMet: 1.202 ± 0.031	1.282ThrAsn: 1.282 ± 0.032	3.661ThrPro: 3.661 ± 0.057	1.736ThrGln: 1.736 ± 0.032	3.583ThrArg: 3.583 ± 0.049	2.619ThrSer: 2.619 ± 0.05	3.163ThrThr: 3.163 ± 0.072	4.502ThrVal: 4.502 ± 0.084	0.739ThrTrp: 0.739 ± 0.026	1.089ThrTyr: 1.089 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	10.382ValAla: 10.382 ± 0.094	0.699ValCys: 0.699 ± 0.025	4.559ValAsp: 4.559 ± 0.061	4.186ValGlu: 4.186 ± 0.075	2.808ValPhe: 2.808 ± 0.048	6.017ValGly: 6.017 ± 0.076	1.607ValHis: 1.607 ± 0.034	3.576ValIle: 3.576 ± 0.055	2.471ValLys: 2.471 ± 0.046	8.449ValLeu: 8.449 ± 0.086	1.903ValMet: 1.903 ± 0.038	2.06ValAsn: 2.06 ± 0.047	4.263ValPro: 4.263 ± 0.062	2.704ValGln: 2.704 ± 0.043	5.393ValArg: 5.393 ± 0.069	4.145ValSer: 4.145 ± 0.061	4.405ValThr: 4.405 ± 0.064	6.718ValVal: 6.718 ± 0.085	1.05ValTrp: 1.05 ± 0.025	1.537ValTyr: 1.537 ± 0.035	0.001ValXaa: 0.001 ± 0.001
Trp	1.497TrpAla: 1.497 ± 0.03	0.177TrpCys: 0.177 ± 0.01	0.639TrpAsp: 0.639 ± 0.017	0.487TrpGlu: 0.487 ± 0.019	0.577TrpPhe: 0.577 ± 0.018	0.941TrpGly: 0.941 ± 0.026	0.362TrpHis: 0.362 ± 0.017	0.657TrpIle: 0.657 ± 0.022	0.436TrpLys: 0.436 ± 0.016	2.016TrpLeu: 2.016 ± 0.044	0.379TrpMet: 0.379 ± 0.017	0.421TrpAsn: 0.421 ± 0.017	0.796TrpPro: 0.796 ± 0.025	0.648TrpGln: 0.648 ± 0.019	1.309TrpArg: 1.309 ± 0.029	0.822TrpSer: 0.822 ± 0.024	0.794TrpThr: 0.794 ± 0.023	1.048TrpVal: 1.048 ± 0.028	0.289TrpTrp: 0.289 ± 0.015	0.269TrpTyr: 0.269 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.543TyrAla: 2.543 ± 0.041	0.216TyrCys: 0.216 ± 0.011	1.112TyrAsp: 1.112 ± 0.032	1.078TyrGlu: 1.078 ± 0.027	0.848TyrPhe: 0.848 ± 0.024	1.941TyrGly: 1.941 ± 0.042	0.391TyrHis: 0.391 ± 0.015	0.743TyrIle: 0.743 ± 0.02	0.619TyrLys: 0.619 ± 0.023	2.257TyrLeu: 2.257 ± 0.043	0.37TyrMet: 0.37 ± 0.016	0.53TyrAsn: 0.53 ± 0.02	1.004TyrPro: 1.004 ± 0.03	0.706TyrGln: 0.706 ± 0.021	1.693TyrArg: 1.693 ± 0.036	1.092TyrSer: 1.092 ± 0.026	1.179TyrThr: 1.179 ± 0.026	1.631TyrVal: 1.631 ± 0.031	0.341TyrTrp: 0.341 ± 0.017	0.532TyrTyr: 0.532 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4707 proteins (1567174 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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