Amino acid dipepetide frequency for Enterococcus phage vB_EfaS_IME197

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.837AlaAla: 3.837 ± 0.822
0.408AlaCys: 0.408 ± 0.184
3.511AlaAsp: 3.511 ± 0.51
4.572AlaGlu: 4.572 ± 0.639
2.204AlaPhe: 2.204 ± 0.421
3.511AlaGly: 3.511 ± 0.614
0.898AlaHis: 0.898 ± 0.257
6.042AlaIle: 6.042 ± 0.837
5.96AlaLys: 5.96 ± 0.631
4.98AlaLeu: 4.98 ± 0.683
2.041AlaMet: 2.041 ± 0.557
3.511AlaAsn: 3.511 ± 0.682
0.816AlaPro: 0.816 ± 0.228
2.286AlaGln: 2.286 ± 0.402
2.286AlaArg: 2.286 ± 0.42
3.674AlaSer: 3.674 ± 0.463
3.919AlaThr: 3.919 ± 0.627
4.082AlaVal: 4.082 ± 0.542
0.816AlaTrp: 0.816 ± 0.228
2.204AlaTyr: 2.204 ± 0.411
0.0AlaXaa: 0.0 ± 0.0
Cys
0.327CysAla: 0.327 ± 0.161
0.082CysCys: 0.082 ± 0.098
0.163CysAsp: 0.163 ± 0.115
0.49CysGlu: 0.49 ± 0.211
0.163CysPhe: 0.163 ± 0.112
0.572CysGly: 0.572 ± 0.263
0.163CysHis: 0.163 ± 0.129
0.98CysIle: 0.98 ± 0.311
0.898CysLys: 0.898 ± 0.304
0.327CysLeu: 0.327 ± 0.157
0.163CysMet: 0.163 ± 0.115
0.327CysAsn: 0.327 ± 0.157
0.082CysPro: 0.082 ± 0.081
0.245CysGln: 0.245 ± 0.137
0.245CysArg: 0.245 ± 0.144
0.49CysSer: 0.49 ± 0.176
0.082CysThr: 0.082 ± 0.072
0.327CysVal: 0.327 ± 0.151
0.163CysTrp: 0.163 ± 0.103
0.327CysTyr: 0.327 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
4.327AspAla: 4.327 ± 0.585
0.245AspCys: 0.245 ± 0.139
4.001AspAsp: 4.001 ± 0.562
5.552AspGlu: 5.552 ± 0.536
3.184AspPhe: 3.184 ± 0.371
3.429AspGly: 3.429 ± 0.535
0.898AspHis: 0.898 ± 0.312
4.817AspIle: 4.817 ± 0.792
5.552AspLys: 5.552 ± 0.713
4.246AspLeu: 4.246 ± 0.607
1.225AspMet: 1.225 ± 0.244
3.919AspAsn: 3.919 ± 0.494
1.388AspPro: 1.388 ± 0.331
1.715AspGln: 1.715 ± 0.308
2.041AspArg: 2.041 ± 0.358
3.429AspSer: 3.429 ± 0.594
3.756AspThr: 3.756 ± 0.577
4.001AspVal: 4.001 ± 0.493
0.49AspTrp: 0.49 ± 0.182
2.939AspTyr: 2.939 ± 0.444
0.0AspXaa: 0.0 ± 0.0
Glu
5.96GluAla: 5.96 ± 0.697
0.408GluCys: 0.408 ± 0.176
3.837GluAsp: 3.837 ± 0.718
5.96GluGlu: 5.96 ± 0.787
3.511GluPhe: 3.511 ± 0.525
3.103GluGly: 3.103 ± 0.572
1.551GluHis: 1.551 ± 0.263
6.205GluIle: 6.205 ± 0.763
7.92GluLys: 7.92 ± 0.682
7.838GluLeu: 7.838 ± 0.814
2.531GluMet: 2.531 ± 0.356
5.389GluAsn: 5.389 ± 0.723
1.388GluPro: 1.388 ± 0.314
3.429GluGln: 3.429 ± 0.633
3.184GluArg: 3.184 ± 0.488
3.184GluSer: 3.184 ± 0.4
4.001GluThr: 4.001 ± 0.545
4.654GluVal: 4.654 ± 0.589
0.898GluTrp: 0.898 ± 0.268
3.184GluTyr: 3.184 ± 0.466
0.0GluXaa: 0.0 ± 0.0
Phe
1.96PheAla: 1.96 ± 0.372
0.163PheCys: 0.163 ± 0.127
3.103PheAsp: 3.103 ± 0.444
3.429PheGlu: 3.429 ± 0.557
1.388PhePhe: 1.388 ± 0.309
2.613PheGly: 2.613 ± 0.476
0.408PheHis: 0.408 ± 0.145
3.021PheIle: 3.021 ± 0.609
4.164PheLys: 4.164 ± 0.602
2.531PheLeu: 2.531 ± 0.575
1.061PheMet: 1.061 ± 0.25
3.511PheAsn: 3.511 ± 0.542
0.898PhePro: 0.898 ± 0.275
1.47PheGln: 1.47 ± 0.462
1.306PheArg: 1.306 ± 0.3
3.021PheSer: 3.021 ± 0.441
2.368PheThr: 2.368 ± 0.386
1.96PheVal: 1.96 ± 0.405
0.327PheTrp: 0.327 ± 0.163
1.388PheTyr: 1.388 ± 0.371
0.0PheXaa: 0.0 ± 0.0
Gly
3.184GlyAla: 3.184 ± 0.565
0.408GlyCys: 0.408 ± 0.165
2.613GlyAsp: 2.613 ± 0.39
3.674GlyGlu: 3.674 ± 0.653
1.796GlyPhe: 1.796 ± 0.343
3.021GlyGly: 3.021 ± 0.937
0.816GlyHis: 0.816 ± 0.31
4.817GlyIle: 4.817 ± 0.742
5.389GlyLys: 5.389 ± 0.687
5.062GlyLeu: 5.062 ± 0.657
1.388GlyMet: 1.388 ± 0.272
3.347GlyAsn: 3.347 ± 0.562
0.898GlyPro: 0.898 ± 0.285
1.878GlyGln: 1.878 ± 0.438
2.123GlyArg: 2.123 ± 0.429
3.184GlySer: 3.184 ± 0.608
2.858GlyThr: 2.858 ± 0.454
4.246GlyVal: 4.246 ± 0.804
0.49GlyTrp: 0.49 ± 0.226
3.756GlyTyr: 3.756 ± 0.674
0.0GlyXaa: 0.0 ± 0.0
His
0.163HisAla: 0.163 ± 0.098
0.082HisCys: 0.082 ± 0.081
0.816HisAsp: 0.816 ± 0.218
0.735HisGlu: 0.735 ± 0.181
0.49HisPhe: 0.49 ± 0.195
0.735HisGly: 0.735 ± 0.247
0.572HisHis: 0.572 ± 0.201
1.061HisIle: 1.061 ± 0.324
0.408HisLys: 0.408 ± 0.216
0.98HisLeu: 0.98 ± 0.332
0.082HisMet: 0.082 ± 0.087
0.98HisAsn: 0.98 ± 0.332
0.163HisPro: 0.163 ± 0.111
0.572HisGln: 0.572 ± 0.203
0.327HisArg: 0.327 ± 0.189
1.306HisSer: 1.306 ± 0.375
1.388HisThr: 1.388 ± 0.413
0.98HisVal: 0.98 ± 0.307
0.245HisTrp: 0.245 ± 0.119
1.143HisTyr: 1.143 ± 0.301
0.0HisXaa: 0.0 ± 0.0
Ile
4.654IleAla: 4.654 ± 0.843
0.572IleCys: 0.572 ± 0.196
5.715IleAsp: 5.715 ± 0.706
6.695IleGlu: 6.695 ± 0.648
2.041IlePhe: 2.041 ± 0.467
3.184IleGly: 3.184 ± 0.475
0.735IleHis: 0.735 ± 0.203
4.001IleIle: 4.001 ± 0.638
6.858IleLys: 6.858 ± 0.599
4.899IleLeu: 4.899 ± 0.83
0.898IleMet: 0.898 ± 0.286
5.225IleAsn: 5.225 ± 0.649
3.429IlePro: 3.429 ± 0.541
3.756IleGln: 3.756 ± 0.487
2.776IleArg: 2.776 ± 0.522
5.715IleSer: 5.715 ± 0.666
5.389IleThr: 5.389 ± 0.736
3.674IleVal: 3.674 ± 0.609
0.735IleTrp: 0.735 ± 0.256
2.531IleTyr: 2.531 ± 0.486
0.0IleXaa: 0.0 ± 0.0
Lys
5.47LysAla: 5.47 ± 0.747
1.061LysCys: 1.061 ± 0.288
5.47LysAsp: 5.47 ± 0.645
7.92LysGlu: 7.92 ± 0.787
3.511LysPhe: 3.511 ± 0.441
4.899LysGly: 4.899 ± 0.705
0.98LysHis: 0.98 ± 0.286
6.205LysIle: 6.205 ± 0.965
10.287LysLys: 10.287 ± 1.338
6.532LysLeu: 6.532 ± 0.691
3.347LysMet: 3.347 ± 0.567
5.389LysAsn: 5.389 ± 0.62
2.368LysPro: 2.368 ± 0.386
4.491LysGln: 4.491 ± 0.632
3.837LysArg: 3.837 ± 0.572
5.715LysSer: 5.715 ± 0.573
6.858LysThr: 6.858 ± 0.677
5.062LysVal: 5.062 ± 0.571
0.735LysTrp: 0.735 ± 0.201
3.511LysTyr: 3.511 ± 0.55
0.0LysXaa: 0.0 ± 0.0
Leu
5.307LeuAla: 5.307 ± 0.666
0.408LeuCys: 0.408 ± 0.171
5.715LeuAsp: 5.715 ± 0.512
5.797LeuGlu: 5.797 ± 0.682
3.021LeuPhe: 3.021 ± 0.475
4.654LeuGly: 4.654 ± 0.508
0.735LeuHis: 0.735 ± 0.27
5.062LeuIle: 5.062 ± 0.776
8.328LeuLys: 8.328 ± 0.735
5.715LeuLeu: 5.715 ± 0.659
2.613LeuMet: 2.613 ± 0.515
6.123LeuAsn: 6.123 ± 0.656
2.613LeuPro: 2.613 ± 0.418
2.368LeuGln: 2.368 ± 0.36
3.103LeuArg: 3.103 ± 0.513
6.45LeuSer: 6.45 ± 0.838
4.001LeuThr: 4.001 ± 0.598
4.001LeuVal: 4.001 ± 0.507
0.735LeuTrp: 0.735 ± 0.193
2.613LeuTyr: 2.613 ± 0.416
0.0LeuXaa: 0.0 ± 0.0
Met
1.388MetAla: 1.388 ± 0.315
0.163MetCys: 0.163 ± 0.117
1.878MetAsp: 1.878 ± 0.398
1.47MetGlu: 1.47 ± 0.328
0.816MetPhe: 0.816 ± 0.265
1.225MetGly: 1.225 ± 0.28
0.327MetHis: 0.327 ± 0.149
2.204MetIle: 2.204 ± 0.378
2.531MetLys: 2.531 ± 0.515
2.286MetLeu: 2.286 ± 0.543
0.245MetMet: 0.245 ± 0.128
1.715MetAsn: 1.715 ± 0.376
0.898MetPro: 0.898 ± 0.271
1.47MetGln: 1.47 ± 0.365
1.225MetArg: 1.225 ± 0.293
1.796MetSer: 1.796 ± 0.37
1.47MetThr: 1.47 ± 0.407
1.143MetVal: 1.143 ± 0.273
0.327MetTrp: 0.327 ± 0.149
1.061MetTyr: 1.061 ± 0.292
0.0MetXaa: 0.0 ± 0.0
Asn
4.246AsnAla: 4.246 ± 0.601
0.572AsnCys: 0.572 ± 0.211
2.694AsnAsp: 2.694 ± 0.389
5.307AsnGlu: 5.307 ± 0.724
2.694AsnPhe: 2.694 ± 0.52
5.225AsnGly: 5.225 ± 0.51
1.143AsnHis: 1.143 ± 0.395
4.735AsnIle: 4.735 ± 0.711
5.879AsnLys: 5.879 ± 0.572
4.98AsnLeu: 4.98 ± 0.498
1.143AsnMet: 1.143 ± 0.261
3.592AsnAsn: 3.592 ± 0.622
2.449AsnPro: 2.449 ± 0.502
3.756AsnGln: 3.756 ± 0.726
2.858AsnArg: 2.858 ± 0.497
3.592AsnSer: 3.592 ± 0.747
3.837AsnThr: 3.837 ± 0.581
3.837AsnVal: 3.837 ± 0.565
0.572AsnTrp: 0.572 ± 0.199
2.368AsnTyr: 2.368 ± 0.419
0.0AsnXaa: 0.0 ± 0.0
Pro
1.388ProAla: 1.388 ± 0.331
0.082ProCys: 0.082 ± 0.081
1.715ProAsp: 1.715 ± 0.453
2.286ProGlu: 2.286 ± 0.439
1.715ProPhe: 1.715 ± 0.383
0.98ProGly: 0.98 ± 0.301
0.327ProHis: 0.327 ± 0.144
1.878ProIle: 1.878 ± 0.335
3.266ProLys: 3.266 ± 0.568
2.204ProLeu: 2.204 ± 0.399
0.98ProMet: 0.98 ± 0.26
2.123ProAsn: 2.123 ± 0.446
0.653ProPro: 0.653 ± 0.194
1.061ProGln: 1.061 ± 0.304
0.653ProArg: 0.653 ± 0.27
1.878ProSer: 1.878 ± 0.421
1.47ProThr: 1.47 ± 0.383
1.96ProVal: 1.96 ± 0.503
0.082ProTrp: 0.082 ± 0.085
1.388ProTyr: 1.388 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
3.184GlnAla: 3.184 ± 0.43
0.327GlnCys: 0.327 ± 0.155
1.551GlnAsp: 1.551 ± 0.375
3.674GlnGlu: 3.674 ± 0.472
1.551GlnPhe: 1.551 ± 0.35
1.633GlnGly: 1.633 ± 0.401
0.49GlnHis: 0.49 ± 0.171
2.368GlnIle: 2.368 ± 0.462
2.776GlnLys: 2.776 ± 0.552
4.001GlnLeu: 4.001 ± 0.556
1.061GlnMet: 1.061 ± 0.35
2.613GlnAsn: 2.613 ± 0.374
1.061GlnPro: 1.061 ± 0.388
2.531GlnGln: 2.531 ± 0.643
1.796GlnArg: 1.796 ± 0.318
3.511GlnSer: 3.511 ± 0.532
2.286GlnThr: 2.286 ± 0.404
1.878GlnVal: 1.878 ± 0.31
0.735GlnTrp: 0.735 ± 0.219
1.633GlnTyr: 1.633 ± 0.494
0.0GlnXaa: 0.0 ± 0.0
Arg
1.551ArgAla: 1.551 ± 0.362
0.082ArgCys: 0.082 ± 0.098
1.878ArgAsp: 1.878 ± 0.341
2.613ArgGlu: 2.613 ± 0.46
2.123ArgPhe: 2.123 ± 0.464
1.796ArgGly: 1.796 ± 0.441
0.49ArgHis: 0.49 ± 0.166
3.021ArgIle: 3.021 ± 0.483
3.756ArgLys: 3.756 ± 0.691
3.592ArgLeu: 3.592 ± 0.546
1.143ArgMet: 1.143 ± 0.25
2.858ArgAsn: 2.858 ± 0.55
0.49ArgPro: 0.49 ± 0.169
1.143ArgGln: 1.143 ± 0.329
2.041ArgArg: 2.041 ± 0.396
2.286ArgSer: 2.286 ± 0.476
2.613ArgThr: 2.613 ± 0.491
2.204ArgVal: 2.204 ± 0.37
0.245ArgTrp: 0.245 ± 0.19
1.796ArgTyr: 1.796 ± 0.423
0.0ArgXaa: 0.0 ± 0.0
Ser
3.429SerAla: 3.429 ± 0.523
0.653SerCys: 0.653 ± 0.222
4.164SerAsp: 4.164 ± 0.497
5.144SerGlu: 5.144 ± 0.811
2.776SerPhe: 2.776 ± 0.372
4.001SerGly: 4.001 ± 0.654
1.061SerHis: 1.061 ± 0.406
4.98SerIle: 4.98 ± 0.596
5.225SerLys: 5.225 ± 0.93
5.96SerLeu: 5.96 ± 0.798
2.041SerMet: 2.041 ± 0.436
3.592SerAsn: 3.592 ± 0.655
1.878SerPro: 1.878 ± 0.392
2.368SerGln: 2.368 ± 0.499
2.123SerArg: 2.123 ± 0.333
5.634SerSer: 5.634 ± 0.984
4.98SerThr: 4.98 ± 0.925
3.837SerVal: 3.837 ± 0.426
1.225SerTrp: 1.225 ± 0.283
2.531SerTyr: 2.531 ± 0.465
0.0SerXaa: 0.0 ± 0.0
Thr
4.409ThrAla: 4.409 ± 0.872
0.163ThrCys: 0.163 ± 0.11
4.246ThrAsp: 4.246 ± 0.66
4.654ThrGlu: 4.654 ± 0.705
2.858ThrPhe: 2.858 ± 0.467
4.082ThrGly: 4.082 ± 0.653
0.816ThrHis: 0.816 ± 0.286
4.654ThrIle: 4.654 ± 0.52
4.654ThrLys: 4.654 ± 0.64
5.715ThrLeu: 5.715 ± 0.692
1.388ThrMet: 1.388 ± 0.343
3.511ThrAsn: 3.511 ± 0.52
2.531ThrPro: 2.531 ± 0.576
2.123ThrGln: 2.123 ± 0.419
1.225ThrArg: 1.225 ± 0.265
4.409ThrSer: 4.409 ± 0.58
3.837ThrThr: 3.837 ± 0.6
4.327ThrVal: 4.327 ± 0.643
0.653ThrTrp: 0.653 ± 0.242
1.796ThrTyr: 1.796 ± 0.444
0.0ThrXaa: 0.0 ± 0.0
Val
4.001ValAla: 4.001 ± 0.561
0.572ValCys: 0.572 ± 0.207
5.062ValAsp: 5.062 ± 0.545
4.327ValGlu: 4.327 ± 0.6
1.715ValPhe: 1.715 ± 0.368
3.429ValGly: 3.429 ± 0.495
0.49ValHis: 0.49 ± 0.182
3.837ValIle: 3.837 ± 0.616
5.47ValLys: 5.47 ± 0.615
3.919ValLeu: 3.919 ± 0.577
0.98ValMet: 0.98 ± 0.239
4.491ValAsn: 4.491 ± 0.623
1.96ValPro: 1.96 ± 0.371
1.551ValGln: 1.551 ± 0.422
2.368ValArg: 2.368 ± 0.447
4.654ValSer: 4.654 ± 0.74
3.919ValThr: 3.919 ± 0.542
4.001ValVal: 4.001 ± 0.586
0.653ValTrp: 0.653 ± 0.217
2.204ValTyr: 2.204 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
0.816TrpAla: 0.816 ± 0.27
0.0TrpCys: 0.0 ± 0.0
0.245TrpAsp: 0.245 ± 0.141
0.98TrpGlu: 0.98 ± 0.236
0.408TrpPhe: 0.408 ± 0.141
0.653TrpGly: 0.653 ± 0.208
0.163TrpHis: 0.163 ± 0.122
1.143TrpIle: 1.143 ± 0.317
0.572TrpLys: 0.572 ± 0.228
0.816TrpLeu: 0.816 ± 0.225
0.327TrpMet: 0.327 ± 0.173
0.572TrpAsn: 0.572 ± 0.166
0.245TrpPro: 0.245 ± 0.15
0.572TrpGln: 0.572 ± 0.196
0.408TrpArg: 0.408 ± 0.224
0.735TrpSer: 0.735 ± 0.247
0.408TrpThr: 0.408 ± 0.153
0.98TrpVal: 0.98 ± 0.26
0.245TrpTrp: 0.245 ± 0.138
0.327TrpTyr: 0.327 ± 0.146
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.96TyrAla: 1.96 ± 0.397
0.163TyrCys: 0.163 ± 0.122
2.776TyrAsp: 2.776 ± 0.482
2.939TyrGlu: 2.939 ± 0.571
2.123TyrPhe: 2.123 ± 0.418
2.204TyrGly: 2.204 ± 0.568
0.163TyrHis: 0.163 ± 0.122
2.449TyrIle: 2.449 ± 0.408
3.756TyrLys: 3.756 ± 0.737
2.776TyrLeu: 2.776 ± 0.51
0.898TyrMet: 0.898 ± 0.25
2.694TyrAsn: 2.694 ± 0.401
1.878TyrPro: 1.878 ± 0.451
1.878TyrGln: 1.878 ± 0.443
1.796TyrArg: 1.796 ± 0.447
2.939TyrSer: 2.939 ± 0.44
2.613TyrThr: 2.613 ± 0.487
2.449TyrVal: 2.449 ± 0.452
0.245TyrTrp: 0.245 ± 0.141
1.633TyrTyr: 1.633 ± 0.448
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12249 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski