Halieaceae bacterium IMCC3088

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Aequoribacter

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3L401|F3L401_9GAMM GMP synthase [glutamine-hydrolyzing] OS=Halieaceae bacterium IMCC3088 OX=2518989 GN=guaA PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84AIFLIFVGLLHH13 pKa = 7.28AYY15 pKa = 9.09SANAQEE21 pKa = 4.59AFVLGEE27 pKa = 3.97GRR29 pKa = 11.84LGEE32 pKa = 4.48SVWASDD38 pKa = 5.06LDD40 pKa = 3.98GDD42 pKa = 5.73LIADD46 pKa = 4.5NKK48 pKa = 10.98DD49 pKa = 3.32QFPNAPIGNLTDD61 pKa = 3.46TDD63 pKa = 4.21ADD65 pKa = 3.99GAPNNCDD72 pKa = 3.16QACIDD77 pKa = 3.28SGMRR81 pKa = 11.84ADD83 pKa = 5.82ADD85 pKa = 3.9DD86 pKa = 5.85DD87 pKa = 4.37NDD89 pKa = 3.87GVADD93 pKa = 4.14SEE95 pKa = 5.03DD96 pKa = 4.19AFPLDD101 pKa = 3.35PAEE104 pKa = 4.35TVDD107 pKa = 3.68TDD109 pKa = 4.75GDD111 pKa = 4.2GTGNNADD118 pKa = 4.92LDD120 pKa = 4.41DD121 pKa = 6.16DD122 pKa = 4.98GDD124 pKa = 4.26GYY126 pKa = 11.64ADD128 pKa = 4.16NLDD131 pKa = 4.4AFPIDD136 pKa = 3.9PLEE139 pKa = 4.93HH140 pKa = 7.03SDD142 pKa = 4.91NDD144 pKa = 3.77DD145 pKa = 4.32DD146 pKa = 6.4GIGDD150 pKa = 3.98VADD153 pKa = 5.52DD154 pKa = 5.42DD155 pKa = 5.3DD156 pKa = 6.71DD157 pKa = 5.65NDD159 pKa = 5.4AITDD163 pKa = 3.59ADD165 pKa = 4.51EE166 pKa = 4.38ILLGRR171 pKa = 11.84SPTRR175 pKa = 11.84PEE177 pKa = 4.02YY178 pKa = 11.14VFSTDD183 pKa = 3.1NQSASICLLDD193 pKa = 3.68GEE195 pKa = 5.12RR196 pKa = 11.84IPRR199 pKa = 11.84CWGSNSYY206 pKa = 10.67GQIQPPTSTAFKK218 pKa = 10.28QISLGEE224 pKa = 3.92RR225 pKa = 11.84HH226 pKa = 6.03GCGITLSDD234 pKa = 4.56TVSCWGDD241 pKa = 3.19NAYY244 pKa = 10.21GQASPPEE251 pKa = 4.14NLDD254 pKa = 3.22KK255 pKa = 11.33VKK257 pKa = 10.7QLTSANRR264 pKa = 11.84YY265 pKa = 9.13SCALLEE271 pKa = 5.21DD272 pKa = 5.11DD273 pKa = 6.49SITCWGGKK281 pKa = 10.29YY282 pKa = 10.48YY283 pKa = 10.14MNPLTFSGAKK293 pKa = 9.82KK294 pKa = 9.9IVSGWDD300 pKa = 3.32HH301 pKa = 6.65SCVLHH306 pKa = 6.78SGGISCFGSTGSEE319 pKa = 4.03QIPSDD324 pKa = 3.6LSGSYY329 pKa = 10.44TDD331 pKa = 3.04VHH333 pKa = 6.34AANDD337 pKa = 4.33FVCSEE342 pKa = 4.83DD343 pKa = 3.61STGSTSCYY351 pKa = 10.47GIGKK355 pKa = 9.56DD356 pKa = 2.86IYY358 pKa = 11.08YY359 pKa = 10.63DD360 pKa = 3.54VTSEE364 pKa = 4.19TNINNLTVGSYY375 pKa = 10.36NYY377 pKa = 9.92CSHH380 pKa = 7.01EE381 pKa = 4.07YY382 pKa = 10.82GYY384 pKa = 11.72GLACGGSSSFDD395 pKa = 3.03ILTPPLLNARR405 pKa = 11.84IGTLALTTTMACVLTSTGVTCWGTGANDD433 pKa = 3.38APEE436 pKa = 4.06ILFDD440 pKa = 4.2NDD442 pKa = 2.74QDD444 pKa = 4.01GVNDD448 pKa = 3.96IEE450 pKa = 5.43DD451 pKa = 4.52AFPLDD456 pKa = 3.52AAEE459 pKa = 6.47SIDD462 pKa = 3.83TDD464 pKa = 3.56NDD466 pKa = 4.78GIGNNTDD473 pKa = 4.44LDD475 pKa = 4.43DD476 pKa = 6.4DD477 pKa = 4.27NDD479 pKa = 4.63GYY481 pKa = 11.62SDD483 pKa = 3.87AYY485 pKa = 10.01EE486 pKa = 4.28LEE488 pKa = 4.71KK489 pKa = 10.94GTDD492 pKa = 3.6PLDD495 pKa = 3.81ATDD498 pKa = 6.05FKK500 pKa = 11.17PLYY503 pKa = 9.99DD504 pKa = 4.35DD505 pKa = 4.83LNGIVYY511 pKa = 9.41HH512 pKa = 6.66WSQHH516 pKa = 4.51SLMNAAEE523 pKa = 4.02IHH525 pKa = 6.58RR526 pKa = 11.84ISDD529 pKa = 3.92AEE531 pKa = 4.39VKK533 pKa = 10.54SATNAQGRR541 pKa = 11.84YY542 pKa = 8.69RR543 pKa = 11.84FEE545 pKa = 4.27DD546 pKa = 3.91TPEE549 pKa = 3.9GNYY552 pKa = 9.93EE553 pKa = 4.1LSASQAVTDD562 pKa = 4.0ADD564 pKa = 3.49INRR567 pKa = 11.84TITSADD573 pKa = 3.05ALAALKK579 pKa = 10.3IAVGLNPNSDD589 pKa = 3.8PDD591 pKa = 3.94GDD593 pKa = 4.37GPLEE597 pKa = 4.15ALAVSPYY604 pKa = 10.64QLISADD610 pKa = 3.64MNQDD614 pKa = 2.96GRR616 pKa = 11.84VTSADD621 pKa = 2.99ALAILKK627 pKa = 9.75VAVGLSDD634 pKa = 4.17ALEE637 pKa = 4.42PSWKK641 pKa = 10.15LVADD645 pKa = 4.29AQSLWSTHH653 pKa = 4.97GDD655 pKa = 3.03KK656 pKa = 11.57SNVFDD661 pKa = 4.36ASKK664 pKa = 10.73AYY666 pKa = 10.82LLTYY670 pKa = 9.58PDD672 pKa = 3.34KK673 pKa = 11.38TEE675 pKa = 3.95VNFAAILLGDD685 pKa = 4.48VNPTWKK691 pKa = 9.4PQEE694 pKa = 4.08GTEE697 pKa = 4.4SLNHH701 pKa = 6.42DD702 pKa = 4.56HH703 pKa = 7.31FSMYY707 pKa = 10.76AKK709 pKa = 10.68ASGAPLSLWGIRR721 pKa = 11.84DD722 pKa = 3.41SDD724 pKa = 3.89EE725 pKa = 5.75DD726 pKa = 3.95GLSDD730 pKa = 3.99EE731 pKa = 4.66QEE733 pKa = 4.28EE734 pKa = 4.59TLGTSPLEE742 pKa = 4.41ADD744 pKa = 3.36TDD746 pKa = 4.02ADD748 pKa = 3.83GVNDD752 pKa = 3.74VDD754 pKa = 6.3DD755 pKa = 5.78LFPLDD760 pKa = 4.6PDD762 pKa = 3.8KK763 pKa = 11.62SDD765 pKa = 5.33DD766 pKa = 3.67IPVGAEE772 pKa = 3.86PEE774 pKa = 4.27PVLLASPMKK783 pKa = 10.66ANTSAITMVKK793 pKa = 9.8PVLLRR798 pKa = 11.84GDD800 pKa = 3.61MNDD803 pKa = 3.02WGVSDD808 pKa = 4.67TFDD811 pKa = 3.53EE812 pKa = 5.13LDD814 pKa = 3.98DD815 pKa = 3.91GTYY818 pKa = 9.97QLVMKK823 pKa = 7.21LTPGTYY829 pKa = 7.71TFKK832 pKa = 10.74VASANWSIMDD842 pKa = 4.95LGAQTEE848 pKa = 4.28THH850 pKa = 6.62RR851 pKa = 11.84LIKK854 pKa = 10.58PEE856 pKa = 4.02VPTLLSSEE864 pKa = 4.18PSLPYY869 pKa = 10.59VLVLINEE876 pKa = 4.21TTIVFKK882 pKa = 10.44LTTGEE887 pKa = 3.95HH888 pKa = 4.89KK889 pKa = 10.37KK890 pKa = 11.1YY891 pKa = 10.72YY892 pKa = 8.57ITVFNLPDD900 pKa = 3.65TYY902 pKa = 11.22DD903 pKa = 3.54GPP905 pKa = 4.24

Molecular weight: 96.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3L0U9|F3L0U9_9GAMM Uncharacterized protein OS=Halieaceae bacterium IMCC3088 OX=2518989 GN=IMCC3088_1041 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34SGRR28 pKa = 11.84QLINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2855

0

2855

911031

35

1596

319.1

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.274 ± 0.044

1.011 ± 0.016

5.905 ± 0.038

6.109 ± 0.039

3.829 ± 0.03

7.508 ± 0.038

2.197 ± 0.025

5.577 ± 0.036

3.84 ± 0.031

10.427 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.019

3.462 ± 0.026

4.354 ± 0.024

4.268 ± 0.029

5.598 ± 0.038

6.314 ± 0.038

5.381 ± 0.03

7.244 ± 0.04

1.37 ± 0.019

2.882 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski