Afipia sp. GAS231

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Afipia; unclassified Afipia

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0J3I5|A0A1H0J3I5_9BRAD Tripartite-type tricarboxylate transporter receptor component TctC OS=Afipia sp. GAS231 OX=1882747 GN=SAMN05444050_3993 PE=3 SV=1
MM1 pKa = 7.66ASDD4 pKa = 4.94LPAFPGFPSPPSPPSPPPGGGTPGLIIALPFLDD37 pKa = 4.74TPPSQATDD45 pKa = 3.22SNGAANAVAEE55 pKa = 4.48GAAVNTLVGITAHH68 pKa = 6.56SVDD71 pKa = 4.76AEE73 pKa = 4.45GQTVTYY79 pKa = 10.72SLGADD84 pKa = 3.27SSGGGFKK91 pKa = 9.65IDD93 pKa = 3.64PATGVVSVADD103 pKa = 3.39SGKK106 pKa = 10.0IDD108 pKa = 4.16FEE110 pKa = 4.44TAPGHH115 pKa = 6.83AYY117 pKa = 9.24TITVNSSDD125 pKa = 3.59GFISGSQNFTIAVTDD140 pKa = 3.68VAPTAPVDD148 pKa = 4.01SNAAANAVTEE158 pKa = 4.27GAAANTTVGVTAASTDD174 pKa = 3.35INGPAVTYY182 pKa = 10.87SLTADD187 pKa = 3.34TSGGGFKK194 pKa = 9.64IDD196 pKa = 3.69PVTGIVTVADD206 pKa = 3.54ATKK209 pKa = 9.84IDD211 pKa = 4.08YY212 pKa = 10.41EE213 pKa = 4.42SAPGHH218 pKa = 6.83AYY220 pKa = 8.76TVTAQASDD228 pKa = 3.56GTLTNSQTFTIAVNDD243 pKa = 3.95VPLSTPVDD251 pKa = 3.58TNAAANNVAEE261 pKa = 4.64GAANGTAAGITAFAFDD277 pKa = 4.73PNGPATTYY285 pKa = 11.16SLTGDD290 pKa = 3.45TSGGGFTINAVTGVVTVADD309 pKa = 4.09FTKK312 pKa = 9.95IDD314 pKa = 4.01YY315 pKa = 8.72EE316 pKa = 4.57SSGAGHH322 pKa = 7.07SYY324 pKa = 10.3TITVQANDD332 pKa = 3.64GLQTTSHH339 pKa = 6.38GFTIGVADD347 pKa = 4.13VAPSTPVDD355 pKa = 3.28SDD357 pKa = 3.52AAANSVLEE365 pKa = 4.23GAANGSTVGVTASSTDD381 pKa = 3.33VNGPAVTYY389 pKa = 10.68SLTGDD394 pKa = 3.4TSGGGFTINAATGVVTVADD413 pKa = 3.81STKK416 pKa = 9.88IDD418 pKa = 3.7YY419 pKa = 10.45EE420 pKa = 4.36SAPGHH425 pKa = 6.83AYY427 pKa = 8.76TVTAQASDD435 pKa = 3.56GTLTNSQTFTIAVANVPPSTPVDD458 pKa = 3.92SNAGANTVAEE468 pKa = 4.54GAAAGSTVGVTASSTDD484 pKa = 3.18INGPAVTYY492 pKa = 10.77SLTGDD497 pKa = 3.4TSGGGFTINAVTGVVTVADD516 pKa = 3.88PTKK519 pKa = 10.02IDD521 pKa = 3.55YY522 pKa = 10.47EE523 pKa = 4.44SAPGHH528 pKa = 6.83AYY530 pKa = 8.76TVTAQASDD538 pKa = 3.69GTASSSQIFIIAVTDD553 pKa = 3.62VAPSTPVDD561 pKa = 3.28SDD563 pKa = 3.52AAANSIAEE571 pKa = 4.37GPANGSTVGVTASSTDD587 pKa = 3.33VNGPAVTYY595 pKa = 10.68SLTGDD600 pKa = 3.4TSGGGFTINATTGVITVADD619 pKa = 3.82STKK622 pKa = 9.94IDD624 pKa = 3.96YY625 pKa = 9.1EE626 pKa = 4.49SSGAGHH632 pKa = 7.06SYY634 pKa = 9.88TVTATASDD642 pKa = 4.06GTQSNSQAFVIDD654 pKa = 3.78VTDD657 pKa = 3.72VAPSTPVDD665 pKa = 3.72SNAAANQVSQGAPVGTVVGLTASSTDD691 pKa = 3.33VNGPAVTYY699 pKa = 10.63SLIGDD704 pKa = 3.99TSGGGFTINAATGVVTVADD723 pKa = 3.94PSKK726 pKa = 11.02VIFNAGSPSFDD737 pKa = 3.2VRR739 pKa = 11.84VDD741 pKa = 3.48SSDD744 pKa = 3.42GTLHH748 pKa = 5.76NQQTFTINVLQNTPPVANDD767 pKa = 3.51DD768 pKa = 3.9SLAATEE774 pKa = 5.29AGGLNNSIAGSNPAGNVILGTGSAGAVQDD803 pKa = 4.3TDD805 pKa = 3.8AQDD808 pKa = 3.51PSNALTVVAVHH819 pKa = 6.59TGPEE823 pKa = 4.3GASTGTGTVNSATVGSHH840 pKa = 5.5GTLTLNSDD848 pKa = 3.22GSYY851 pKa = 10.19TYY853 pKa = 11.06LVNQTDD859 pKa = 3.68AQVQALHH866 pKa = 6.46TSAEE870 pKa = 4.36TITDD874 pKa = 3.45VFNYY878 pKa = 8.64TIQDD882 pKa = 3.41TGGLQDD888 pKa = 3.56TATLTVTIHH897 pKa = 6.44GADD900 pKa = 4.5DD901 pKa = 4.11LPQAVADD908 pKa = 3.96TGTMTEE914 pKa = 4.62DD915 pKa = 4.24DD916 pKa = 4.68APTSFNVIANDD927 pKa = 3.86TQDD930 pKa = 3.88PDD932 pKa = 3.49HH933 pKa = 6.6TASNTITLGPGGVTVTGPGGEE954 pKa = 4.34TFANTDD960 pKa = 2.98ATASIVANQIQITLNNAHH978 pKa = 6.42FQQLALGEE986 pKa = 4.55HH987 pKa = 5.99ATITAPYY994 pKa = 8.91TLTGDD999 pKa = 3.54AGEE1002 pKa = 4.22TSSANLVVTVNGVNDD1017 pKa = 3.47VPVAVDD1023 pKa = 3.32DD1024 pKa = 4.2TGTMTEE1030 pKa = 4.5DD1031 pKa = 3.39QSGHH1035 pKa = 6.32AFTVLANDD1043 pKa = 4.33TLDD1046 pKa = 4.38ADD1048 pKa = 4.0HH1049 pKa = 7.03GAPNNVTVNAATAVTNLSAPSGEE1072 pKa = 4.92GITASDD1078 pKa = 3.05ISVSVNGSNQVVVNLGANFQHH1099 pKa = 6.09MQDD1102 pKa = 4.04GQSSTFDD1109 pKa = 3.18VAYY1112 pKa = 8.94TLHH1115 pKa = 7.18GDD1117 pKa = 3.7QPGDD1121 pKa = 3.12TSTAEE1126 pKa = 3.77LHH1128 pKa = 5.29VTVTGANDD1136 pKa = 3.3APVAGNFTFNGVNSAIGNTDD1156 pKa = 3.56LVVNDD1161 pKa = 4.82PADD1164 pKa = 3.71TAQTATGPAKK1174 pKa = 9.98TISASLLSGASDD1186 pKa = 3.43VDD1188 pKa = 3.96GPGPLIVVAGTIATAHH1204 pKa = 6.32GGAVTMQSDD1213 pKa = 3.71GDD1215 pKa = 4.34FIYY1218 pKa = 10.36KK1219 pKa = 10.23PPVGYY1224 pKa = 8.84TGSDD1228 pKa = 3.13SFNYY1232 pKa = 9.35QVSDD1236 pKa = 3.68QNAGASGVGLGTGTVTINVASPHH1259 pKa = 4.38VWYY1262 pKa = 11.05VNADD1266 pKa = 3.29AATDD1270 pKa = 3.88GDD1272 pKa = 4.25GSSEE1276 pKa = 4.07NPFNSLSHH1284 pKa = 6.38FNGAGGVDD1292 pKa = 3.72GSGDD1296 pKa = 3.88TIVLEE1301 pKa = 4.46TASAHH1306 pKa = 4.28YY1307 pKa = 9.57TGGLTLEE1314 pKa = 4.48NNEE1317 pKa = 3.97QLISQSAGVTINGTTLLAASGANAVVDD1344 pKa = 3.79GGVILGSGNNIQGVDD1359 pKa = 4.38FGATSGFAVSGSSVGTVHH1377 pKa = 7.74IDD1379 pKa = 3.35DD1380 pKa = 4.76SKK1382 pKa = 11.82SGVINNASGGGISIGGSSNVLNVDD1406 pKa = 4.21LSSVTTGGGTNGIVINGASGSFHH1429 pKa = 6.71AHH1431 pKa = 6.58GGTISGASGSDD1442 pKa = 3.35VSLSSGTLNFTDD1454 pKa = 5.21DD1455 pKa = 4.49GSISDD1460 pKa = 3.73NSGSNVSVSSMTGGTQSFTGAISGNGSTGISLTSNTGSTIDD1501 pKa = 3.57FSGGINLSTGASNAFTATGGGTVNVTGTNHH1531 pKa = 6.39LTTTTGTALNINNTTIGSSNVTFHH1555 pKa = 7.67DD1556 pKa = 3.59ISANGAANGIVLNNTGTSGHH1576 pKa = 6.56LAVTGGGSSALGGDD1590 pKa = 3.52NSGGTIQNTTGDD1602 pKa = 4.55GISLTNTSGVTFTNIHH1618 pKa = 4.78VTNTSGDD1625 pKa = 4.13GINGTTVGGFTLANSTVDD1643 pKa = 3.46GANGGSAVNQGSIYY1657 pKa = 9.62FTGLTGTANVTNDD1670 pKa = 3.49VIKK1673 pKa = 10.71GGHH1676 pKa = 5.55QDD1678 pKa = 3.21NVSILTSSGTLDD1690 pKa = 3.47LTVSGSTIRR1699 pKa = 11.84DD1700 pKa = 3.21TDD1702 pKa = 3.14TGTNGNDD1709 pKa = 3.23NLFIEE1714 pKa = 5.06ANGTSTVTAHH1724 pKa = 5.45VQNNTFAATNGDD1736 pKa = 3.63HH1737 pKa = 6.43LQTVGDD1743 pKa = 4.88DD1744 pKa = 3.55SATLNIVATGNTLSGGGGVNALGEE1768 pKa = 4.86GITISGGHH1776 pKa = 6.06SSPSPSTEE1784 pKa = 3.2HH1785 pKa = 6.47VNFNISNNTMTGTIQGGAINVNEE1808 pKa = 4.38GAGSGTWQGQINNNTVGNPAITDD1831 pKa = 3.42SGAAQSSAIRR1841 pKa = 11.84AEE1843 pKa = 3.91NHH1845 pKa = 6.27SNGGALTVLIDD1856 pKa = 4.3GNNLSQFNGVGISLQDD1872 pKa = 3.87GEE1874 pKa = 5.09SSTSTATLNATVTNNVEE1891 pKa = 4.46SNPGSTFNGGPNYY1904 pKa = 10.7GLLLNVGVASGSSNVAHH1921 pKa = 7.16ANIHH1925 pKa = 6.86DD1926 pKa = 4.01NTLVASTSGGFGIRR1940 pKa = 11.84VRR1942 pKa = 11.84EE1943 pKa = 3.95RR1944 pKa = 11.84FGADD1948 pKa = 2.47IFLDD1952 pKa = 3.88NYY1954 pKa = 11.14AGGSTDD1960 pKa = 3.21QTAVANYY1967 pKa = 9.52LVAKK1971 pKa = 10.39NPGNTAVAAMSLGGGATQGGGFFNGTTPTPIVPLQLFAAGGVQASPTTPGEE2022 pKa = 4.15MNLTMSEE2029 pKa = 4.25LNVIVAAAIADD2040 pKa = 3.65WTAAGASAAQIDD2052 pKa = 4.23AMHH2055 pKa = 6.95AASFSIANLSDD2066 pKa = 3.26GVLGQEE2072 pKa = 4.16TPGAIQVSSNAAGNGWFVDD2091 pKa = 3.6ATPAGNSEE2099 pKa = 4.48FPHH2102 pKa = 6.52AANPADD2108 pKa = 4.19TDD2110 pKa = 4.49LLTDD2114 pKa = 4.57PSNAAAGHH2122 pKa = 5.78VDD2124 pKa = 3.8LLTVVMHH2131 pKa = 6.43EE2132 pKa = 4.46LGHH2135 pKa = 7.0IIGIDD2140 pKa = 3.71DD2141 pKa = 3.57TTAPADD2147 pKa = 3.6INDD2150 pKa = 3.83LMFMYY2155 pKa = 10.59LADD2158 pKa = 3.9GEE2160 pKa = 4.62RR2161 pKa = 11.84RR2162 pKa = 11.84LPTASDD2168 pKa = 3.34VGKK2171 pKa = 9.99ADD2173 pKa = 3.7PMSSFQAVDD2182 pKa = 3.65PLAPPPPAPASTTTVAAPAGSQTFDD2207 pKa = 3.61AGHH2210 pKa = 6.83GGQTLVATAGPDD2222 pKa = 3.16TFVFANVDD2230 pKa = 3.24VHH2232 pKa = 7.49APAPPPVTLIEE2243 pKa = 4.47NYY2245 pKa = 10.67SFAQGDD2251 pKa = 3.75KK2252 pKa = 10.6FDD2254 pKa = 4.82FSAITSQFHH2263 pKa = 6.67ASGFGDD2269 pKa = 3.69ALIVRR2274 pKa = 11.84AVEE2277 pKa = 3.86DD2278 pKa = 3.68AGGKK2282 pKa = 9.19FATLQVDD2289 pKa = 3.49TLDD2292 pKa = 3.66PHH2294 pKa = 7.07GLPGMANWVNVAQLDD2309 pKa = 4.13GAHH2312 pKa = 6.88AGDD2315 pKa = 4.22AVNVLIDD2322 pKa = 3.67GQAVHH2327 pKa = 7.15LAQIHH2332 pKa = 5.76VGLLAA2337 pKa = 4.61

Molecular weight: 229.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0G2K8|A0A1H0G2K8_9BRAD Uncharacterized protein OS=Afipia sp. GAS231 OX=1882747 GN=SAMN05444050_2993 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATTGGRR28 pKa = 11.84KK29 pKa = 9.06VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6976

0

6976

2166649

29

7515

310.6

33.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.362 ± 0.037

0.863 ± 0.01

5.483 ± 0.025

5.152 ± 0.032

3.855 ± 0.02

8.507 ± 0.047

2.016 ± 0.014

5.417 ± 0.022

3.794 ± 0.031

9.678 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.016

2.935 ± 0.035

5.249 ± 0.031

3.152 ± 0.014

6.647 ± 0.043

5.766 ± 0.032

5.546 ± 0.047

7.465 ± 0.024

1.333 ± 0.012

2.299 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski